Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the...Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the development of the pharmaceutical industry,the medicinal value of P.oleracea is becoming increasingly prominent.It is commonly used in clinical practice for clearing heat and detoxifying,inhibiting bacteria,preventing and treating diseases such as hypertension,coronary heart disease,and cerebral infarction,indicating its broad application prospects.This study reviews the chemical components and pharmacological activities of P.oleracea in recent decades.展开更多
Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and ant...Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and antiviral agents now. It also decreases bloodsugar, enhances immunity and adjusts blood lipids. The anticancer and antifungal effects ofPortulaca oleracea L. were reported in several patents. But no active component has been found. Inthis study, the chemical compositions from Portulaca oleracea L. were investigated to provideevidence for the activity of Portulaca oleracea L. .展开更多
[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw mate...[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.展开更多
With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oler...With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.展开更多
Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at...Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at all adsorption experiments. Effects of contact time on adsorption capacity of Pb(II) onto Portulaca oleracea leaves were studied. The adsorption capacity increased as the contact time increased and reached equilibrium at one hour. Kinetic models including a first-order, pseudo-second-order and intra-particle diffusion equations were selected to follow the adsorption process. The process follows a pseudo-second-order kinetic and the intra-particle diffusion is the main step to interpret the mechanism of adsorption. Langmuir, Freundlich and Dubinin-Radush Kevich (D-R) isotherm models were applied to describe adsorption equilibrium data. Results proved that the Langmuir isotherm model gave an acceptable fit to the experimental data more than Freundlich isotherm model. Maximum adsorption capacities obtained were 192.3, 333.3 and 434 mg/g at 295, 303 and 310 K, respectively. According to D-R isotherm data, the adsorption process is classified as physical adsorption. Thermodynamically, the adsorption process is non spontaneous, endothermic and random in nature.展开更多
The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a...The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.展开更多
目的基于网络药理学及分子对接技术探讨马齿苋改善肝损伤的有效活性成分及分子机制。方法通过Traditional Chinese Medicine Systems Pharmacology(TCMSP)数据库检索马齿苋的活性化合物及相关靶点;GeneCards、On⁃line Mendelian Inherit...目的基于网络药理学及分子对接技术探讨马齿苋改善肝损伤的有效活性成分及分子机制。方法通过Traditional Chinese Medicine Systems Pharmacology(TCMSP)数据库检索马齿苋的活性化合物及相关靶点;GeneCards、On⁃line Mendelian Inheritance in Man(OMIM)数据库收集肝损伤的相关靶点;通过Venny 2.1.0网站筛选马齿苋与肝损伤的共表达靶点。运用Cytoscape 3.9.1软件和Search Tool for the Retrieval of Interacting Genes/Proteins数据库构建马齿苋活性成分与肝损伤共表达靶点的“药物-主要活性成分-共表达靶点-疾病”网络以及蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络图。通过the Database for Annotation,Visu⁃alization and Integrated Discovery数据库对共表达靶点进行基因本体论(gene ontology,GO)富集和京都基因与基因组百科全书(Kyoto encycloppedia genes and genomes,KEGG)通路注释分析,阐述马齿苋改善肝损伤的作用通路及分子作用机制。通过CB-Dock在线分子对接网站验证马齿苋有效活性成分与关键靶点之间的关系。结果从TCMSP数据库中筛选出10种马齿苋活性化合物及其对应的78个靶点,从GeneCards和OMIM数据库中筛选出1489个肝损伤的相关靶点,通过Venny 2.1.0网站找到54个马齿苋活性成分与肝损伤的共表达靶点。通过Cytoscape 3.9.1软件获得“药物-主要活性成分-共表达靶点-疾病”网络并找出马齿苋改善肝损伤的主要活性成分,在PPI网络图中得到关键靶点为TNF、IL6、IL1B、MMP9、CASP3、IL10、PPARG。GO富集分析结果显示马齿苋主要对转录、凋亡等生物过程起调节作用,通过KEGG通路分析筛选出与马齿苋改善肝损伤关系密切的通路为Non-alcoholic fatty liver disease通路,并且其重要的靶点有TGFB1,INSR,TNF,TNFRSF1A,IL1A,CASP7,IL6,MAPK8,CASP8,IL1B,CASP3,PPARG和PPARA。分子对接结果表明,马齿苋中主要活性成分β-胡萝卜素、槲皮素与核心靶点TNF、CASP3、PPARG稳定结合。结论马齿苋中的β-胡萝卜素、槲皮素通过与TNF、CASP3、PPARG结合调节Non-alcoholic fatty liver disease等信号通路发挥改善肝损伤的作用。展开更多
基金This work was financially supported by National Nature Science Foundation of China(81973284)Scientific Research Foundation of the Education Department of Liaoning Province(LJKZ0944).
文摘Portulaca oleracea(P.oleracea)is a traditional Chinese herbal that has the effects of soothing the liver and clearing collaterals,strengthening the spleen and stomach,moistening the intestines and detoxifying.With the development of the pharmaceutical industry,the medicinal value of P.oleracea is becoming increasingly prominent.It is commonly used in clinical practice for clearing heat and detoxifying,inhibiting bacteria,preventing and treating diseases such as hypertension,coronary heart disease,and cerebral infarction,indicating its broad application prospects.This study reviews the chemical components and pharmacological activities of P.oleracea in recent decades.
文摘Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and antiviral agents now. It also decreases bloodsugar, enhances immunity and adjusts blood lipids. The anticancer and antifungal effects ofPortulaca oleracea L. were reported in several patents. But no active component has been found. Inthis study, the chemical compositions from Portulaca oleracea L. were investigated to provideevidence for the activity of Portulaca oleracea L. .
文摘[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds.
文摘With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.
文摘Portulaca oleracea leaves are tested as an agriculture adsorbent material for reducing Pb(II) from aqueous solutions. The pH of solution, adsorbent dose, shaking speed and particles size of adsorbent were constants at all adsorption experiments. Effects of contact time on adsorption capacity of Pb(II) onto Portulaca oleracea leaves were studied. The adsorption capacity increased as the contact time increased and reached equilibrium at one hour. Kinetic models including a first-order, pseudo-second-order and intra-particle diffusion equations were selected to follow the adsorption process. The process follows a pseudo-second-order kinetic and the intra-particle diffusion is the main step to interpret the mechanism of adsorption. Langmuir, Freundlich and Dubinin-Radush Kevich (D-R) isotherm models were applied to describe adsorption equilibrium data. Results proved that the Langmuir isotherm model gave an acceptable fit to the experimental data more than Freundlich isotherm model. Maximum adsorption capacities obtained were 192.3, 333.3 and 434 mg/g at 295, 303 and 310 K, respectively. According to D-R isotherm data, the adsorption process is classified as physical adsorption. Thermodynamically, the adsorption process is non spontaneous, endothermic and random in nature.
文摘The aim of this study was to investigate the antihyperglycemic and antioxidant effects of Po （Portulaca oleracea） lyophilised aqueous extract in diabetic male Wistar rats. Diabetes was induced intraperitonially by a single injection of STZ （streptozotocin） （60 mg/kg bw （body weight））. Twenty diabetic rats, weighing 263 ± 5 g, were divided into two groups fed a casein diet supplemented or not with Po extract （1 g/kg bw）, for four weeks. Control group （n = 6） received 0.23-0.25 mL of citrate buffer and was fed a standard diet during the experiment. The study was carried out at Oran University, Algeria and the entire experiments lasted from September 2011 to July 2012. Blood was obtained from the abdominal aorta of rats after fasting overnight and standard methods were used for the extraction of spices, determination of glycemia, insulinemia, lipid peroxidation and antioxidant enzymes activities. Portulaca oleracea treated compared to untreated rats, glycemia and HbAIc values were respectively 2.8- and 1.7-fold lower. TBARS （thiobarbituric acid reactive substances） concentrations were reduced in RBC （red blood cells） （-54%） and plasma （-65%）. Moreover, in liver and kidney, TBARS values were respectively 1.8- and 2-fold lower. SOD （superoxide dismutase） and GSH-Px （glutathione peroxidase） activities were increased respectively by ＋38% and ＋85%, in liver. GSSG-Red （glutathione reductase） activity was 1.9-fold higher in kidney, while CAT （catalase） was improved in kidney （＋48%）. In RBCs, SOD, GSH-Px, GSSH-Red and CAT activities were increased by 31%, 42%, 56% and ＋50%, respectively. These data have cast a new light on the actions of Portulaca oleracea and its antioxidant potential benefits in preventing diabetes and its complications.
文摘目的基于网络药理学及分子对接技术探讨马齿苋改善肝损伤的有效活性成分及分子机制。方法通过Traditional Chinese Medicine Systems Pharmacology(TCMSP)数据库检索马齿苋的活性化合物及相关靶点;GeneCards、On⁃line Mendelian Inheritance in Man(OMIM)数据库收集肝损伤的相关靶点;通过Venny 2.1.0网站筛选马齿苋与肝损伤的共表达靶点。运用Cytoscape 3.9.1软件和Search Tool for the Retrieval of Interacting Genes/Proteins数据库构建马齿苋活性成分与肝损伤共表达靶点的“药物-主要活性成分-共表达靶点-疾病”网络以及蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络图。通过the Database for Annotation,Visu⁃alization and Integrated Discovery数据库对共表达靶点进行基因本体论(gene ontology,GO)富集和京都基因与基因组百科全书(Kyoto encycloppedia genes and genomes,KEGG)通路注释分析,阐述马齿苋改善肝损伤的作用通路及分子作用机制。通过CB-Dock在线分子对接网站验证马齿苋有效活性成分与关键靶点之间的关系。结果从TCMSP数据库中筛选出10种马齿苋活性化合物及其对应的78个靶点,从GeneCards和OMIM数据库中筛选出1489个肝损伤的相关靶点,通过Venny 2.1.0网站找到54个马齿苋活性成分与肝损伤的共表达靶点。通过Cytoscape 3.9.1软件获得“药物-主要活性成分-共表达靶点-疾病”网络并找出马齿苋改善肝损伤的主要活性成分,在PPI网络图中得到关键靶点为TNF、IL6、IL1B、MMP9、CASP3、IL10、PPARG。GO富集分析结果显示马齿苋主要对转录、凋亡等生物过程起调节作用,通过KEGG通路分析筛选出与马齿苋改善肝损伤关系密切的通路为Non-alcoholic fatty liver disease通路,并且其重要的靶点有TGFB1,INSR,TNF,TNFRSF1A,IL1A,CASP7,IL6,MAPK8,CASP8,IL1B,CASP3,PPARG和PPARA。分子对接结果表明,马齿苋中主要活性成分β-胡萝卜素、槲皮素与核心靶点TNF、CASP3、PPARG稳定结合。结论马齿苋中的β-胡萝卜素、槲皮素通过与TNF、CASP3、PPARG结合调节Non-alcoholic fatty liver disease等信号通路发挥改善肝损伤的作用。
