AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple(Ananas comosus)

Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRKY genes is poorly understood. Here, we studied the pineapple WRKY gene, AcWRKY28, by generating AcWRKY28 over-expressing transgenic pineapple plants. Overexpression of AcWRKY28 enhanced the salt stress resistance in transgenic pineapple lines. Comparative transcriptome analysis of transgenic and wild-type pineapple plants showed that “plant-pathogen interaction” pathway genes, including 9calcium-dependent protein kinases (CPKs), were up-regulated in AcWRKY28 over-expressing plants. Furthermore, chromatin immunoprecipitation and yeast one-hybrid assays revealed AcCPK12, AcCPK3, AcCPK8, AcCPK1, and AcCPK15 as direct targets of AcWRKY28. Consistently, the study of AcCPK12 over-expressing Arabidopsis lines showed that AcCPK12 enhances salt, drought, and disease resistance. This study shows that AcWRKY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCPK genes.

Optimization of the Pretreatment of the Mixture of Cassava Peelings and Pineapple Fibers Using Response Surface Methodology and a Process Simulator for the Bioethanol Production

The increase in oil prices and greenhouse gas emissions has led to the search for substitutes for fossil fuels. In Cameroon, the abundance of lignocellulosic resources is inherent to agricultural activity. Production of bioethanol remains a challenge given the crystallinity of cellulose and the presence of the complex. The pretreatment aimed to solubilize the lignin fraction and to make cellulose more accessible to the hydrolytic enzymes, was done using the organosolv process. A mathematical modeling was performed to point out the effect of the temperature on the kinetics of the release of the reducing sugars during the pretreatment. Two mathematical model was used, SAEMAN’s model and Response surface methodology. The first show that the kinetic parameters of the hydrolysis of the cellulose and reducing sugar are: 0.05089 min-1, 5358.1461 J·mol-1, 1383.03691 min-1, 51577.6100 J·mol-1 respectively. The second model was used. Temperature is the factor having the most positive influence whereas, ethanol concentration is not an essential factor. To release the maximum, an organosolv pre-treatment of this sub-strate should be carried out at 209.08°C for 47.60 min with an ethanol-water ratio of 24.02%. Organosolv pre-treatment is an effective process for delignification of the lignocellulosic structure.

Effect of Ultrasound and Thermal Pasteurization on Physicochemical Properties and Antioxidant Activity of Juice Extracted from Ripe and Overripe Pineapple

It has become necessary to assess how food processing methods affect qualitative qualities due to the increased consumer awareness of the health benefits of various nutrients in food. In the current study, the effects of ultrasound treatment (37°C, 5 min), pasteurization (90°C, 5 min), and their combination on quality parameters, including pH, total soluble solids (TSSs), titratable acidity (TA), color values (L*, a*, b*), ascorbic acid (AA), total phenolic content (TPC), and antioxidant activity (DPPH), of pineapple juice from ripe and overripe pineapples were assessed. Color values (L*), ascorbic acid (AA), total phenolic content (TPC), and DPPH radical scavenging activity in all juice samples that were sonicated alone and in combination with pasteurization improved significantly (p and color value (a* and b*) decreased in comparison to the control. Whereas pH and acidity did not change significantly (p < 0.05). Pasteurization significantly (p , but sonicated samples significantly (p quality parameters and antioxidant activity, notably in ripe juice. Overall, pasteurization degraded these liquids but sonication, either alone or in combination with it, was advantageous for preserving their quality by retaining nutrients.

Extensive Pineapple Production Constraints and Land Suitability in the Centre Region of Cameroon

The low level of productivity observed in pineapple fields in Centre Came-roon must be sustainably reduced in order to increase producers’ income while using the same resources. The identification and control of production constraints are key steps in optimizing the use of limited resources. To this end, the FAO land assessment methodology following the Fuzzy-MCDM pro-tocol was used for the two pineapple production basins in the Centre, namely Awae and Bokito. It was found that the land in Awae Basin is moderately suitable S2sf with constraints imposed by texture, pH and base saturation. In the Bokito Basin, 25% of the land is suitable S1wf and 75% is moderately suitable S2wsf with constraints imposed by soil texture (27%), temporary soil water saturation (99%), pH, base saturation and exchangeable sodium. Constraint correction improves the land index (potential suitability) and re-mains limited by permanent constraints that cannot be corrected. Improve-ment of the technical itinerary through modification of plant densities, selec-tion of improved cultivars and balanced fertilization must be undertaken to optimize pineapple production in Centre Cameroon.

Epigenetic modification mechanisms of chloroplasts mutants in pineapple leaves during somatic regeneration

Somaclonal variation in tissue culture is a common phenomenon induced by various external or internal environmental conditions,resulting in heritable or non-heritable alterations in gene expression. One crucial mechanism involved in plant growth and development is epigenetic regulation. A highly dynamic epigenome can respond to environmental changes by regulating gene expression. DNA methylation is one of these epigenetic modifications that can alter gene expression in tissue-cultured pineapple plants. The underlying mechanism of such somaclonal variations in pineapple and the epigenetic regulation involvement in somaclonal variations has not been studied. This study performed DNA methylome and transcriptome sequencing of wild-type(WT) and mutant pineapple plants(WS, HW, and TW). We observed altered DNA methylation patterns in chlorophyll development in the mutants. Specifically, we noticed that the methylation levels in the CHG and CHH contexts were lower in the gene body regions compared to the upstream and downstream regions. We identified several thousand differentially methylated regions(DMRs) located at the gene body regions, some of which overlapped with the differentially expressed genes(DEGs). Functional enrichment analyses suggested that these genes were involved in chlorophyll metabolism. Thus, our results revealed that the transcriptional regulation of many chlorophyll metabolic essential genes could be regulated by DNA methylation caused by somaclonal variations and provided insights into epigenetic mechanisms underlying the regulation of chlorosis in pineapple plants.

Comparative Study of Biogas Production from Jackfruit Waste, Banana Peels, and Pineapple Peels Co-Digested with Cow Dung

Only 42% of Uganda’s population has access to electricity. The population continues to use firewood and charcoal as a source of energy, which leads to depletion of forests thus to climate change. The purpose of this study was to assess the potential of biogas production from jackfruit waste, banana peels, and pineapple peels when co-digested with cow dung as an alternative energy source. Substrates for each waste were co-digested with varying proportions (0%, 25%, and 50%) of cow dung using laboratory-scale 250 mL anaerobic digestors. The total biogas generation for jackfruit waste, banana peels, and pineapple peels after 30 days of anaerobic digestion was 82.3, 189, and 262 mL, respectively. When jack fruit waste, pineapple peels and banana peels were co-digested with 25% cow dung, the total amount of biogas produced increased by a factor of two and three, respectively. However, 50% of cow dung only significantly (p ≤ 0.05) improved for jack fruit waste by two folds. Therefore, the results indicated that jackfruit waste, banana and pineapple peels can be used for biogas production to augment energy supply. .

Farmers’ Practices for the Orchard’s Maintenance and Post-Harvest Treatment of Cocoa in Infiltrated Classified and Unclassified Zone of Méagui (South-West, Côte d’Ivoire)

An important part of Ivorian cocoa production comes from infiltrated classified forests. The objective of this study is to investigate orchard maintenance and post-harvest cocoa treatment practices used by cocoa farmers in infiltrated classified and unclassified areas of Méagui. To do this, 110 producers in two localities of the said zone were individually interviewed from February to April 2022, using a declarative questionnaire. It was found that cocoa orchards in the zone are aging. Nearly 68.55% of producers were heirs and 58.33% of those interviewed were under 46 years of age, with an average age of 43 years. Nearly 84.55% of the producers surveyed mainly use chemical insecticides to control the pests of greatest concern in these localities. Regarding the frequency of insecticide treatments, 11.82% of producers make 1 to 2 applications a year, 29.09% make 3 to 5 applications a year, and 43.64% make at least 6 applications a year. The active substances of the most used products belong to 99% of the Neonicotinoid and Pyrethroid families. Regarding cocoa harvesting and post-harvest operations, 93.64% of the producers carry out a single harvest/month;all do the shelling with mini machetes. Beans are fermented mainly in black plastic tarpaulins or in bags, for a period of 3 to 5 days (65.45%). Drying is done almost entirely (91.82%) on the black plastic tarp. This study shows that cocoa farmers in the areas surveyed do not respect the recommendations issued on phytosanitary practices and post-harvest treatment of cocoa. This non-compliance with the standards of good practices prescribed is particularly more accentuated in the locality located in infiltrated classified zone. It would, therefore, be judicious to revise or redefine the phytosanitary recommendations and carry out sensitization and training-follow-up programs for producers in this important cocoa production area of C?te d’Ivoire.

Influence of Harvest Periods on Cassava (Manihot esculenta Crantz) Agronomic Traits and Physiological Response to Post-Harvest Physiological Deterioration

Cassava (Manihot esculenta Crantz) is the third largest source of calories in tropical countries and the sixth most important food crop in the World. However, the short shelf life of its storage roots after harvest due to a rapid post-harvest physiological deterioration (PPD) makes the roots to be considered as a risky product to market. The objectives of this work were to investigate the influence of two harvest periods on cassava agronomic parameters and their physiological response to PPD. Three cassava cultivars 96/1414, I070593 and LMR were selected for the experiment and harvested at 10 and 12 months after planting (MAP). The response to PPD was assessed during storage at 0, 3, 8 and 15 days after harvest (DAH). Total proteins content, soluble sugars and starch, total polyphenols compounds, polyphenoloxidase and peroxidase activities were recorded during storage. Results showed large variation among the parameters at the two harvest periods across the cultivars. High number of tubers was recorded in all the cultivars at 12 MAP and a significant increase in storage roots length was observed in 96/1414 and LMR from 10 MAP to 12 MAP (25 ± 5.1 to 41.3 ± 5.9 and 22.6 ± 3.3 to 27.9 ± 4.8) respectively. A reduction of about 49% of tubers weight was observed in I070593 from 10 to 12 MAP while an increase of about 36% and 11% were recorded in LMR and 96/1414 respectively. Tubers from I070593 showed less susceptibility to PPD when harvested at 10 MAP compared to those from LMR and 96/1414 where less susceptibility to PPD were recorded at 12 MAP. An increase in soluble sugars content, total proteins content and peroxidase activity subsequently to a decrease in starch content were recorded during storage from 8 to 15 days after harvest especially at 10 MAP in I070593 and at 12 MAP in LMR and 96/1414. High content of total phenolic compounds and less activity of polyphenol oxidase were correlated to PPD susceptibility. This work opens a new insight issue of the consideration of the appropriate harvest time of the cultivars as a tool to better control the onset of postharvest physiological deterioration.

Farmers’ Practices for the Orchard’s Maintenance and Post-Harvest Treatment of Cocoa in Infiltrated Classified and Unclassified Zone of Méagui (South-West, Côte d’Ivoire)

An important part of Ivorian cocoa production comes from infiltrated classified forests. The objective of this study is to investigate orchard maintenance and post-harvest cocoa treatment practices used by cocoa farmers in infiltrated classified and unclassified areas of Méagui. To do this, 110 producers in two localities of the said zone were individually interviewed from February to April 2022, using a declarative questionnaire. It was found that cocoa orchards in the zone are aging. Nearly 68.55% of producers were heirs and 58.33% of those interviewed were under 46 years of age, with an average age of 43 years. Nearly 84.55% of the producers surveyed mainly use chemical insecticides to control the pests of greatest concern in these localities. Regarding the frequency of insecticide treatments, 11.82% of producers make 1 to 2 applications a year, 29.09% make 3 to 5 applications a year, and 43.64% make at least 6 applications a year. The active substances of the most used products belong to 99% of the Neonicotinoid and Pyrethroid families. Regarding cocoa harvesting and post-harvest operations, 93.64% of the producers carry out a single harvest/month;all do the shelling with mini machetes. Beans are fermented mainly in black plastic tarpaulins or in bags, for a period of 3 to 5 days (65.45%). Drying is done almost entirely (91.82%) on the black plastic tarp. This study shows that cocoa farmers in the areas surveyed do not respect the recommendations issued on phytosanitary practices and post-harvest treatment of cocoa. This non-compliance with the standards of good practices prescribed is particularly more accentuated in the locality located in infiltrated classified zone. It would, therefore, be judicious to revise or redefine the phytosanitary recommendations and carry out sensitization and training-follow-up programs for producers in this important cocoa production area of C?te d’Ivoire.

Plant Regeneration through Indirect Organogenesis in Two Cultivars of Pineapple (Ananas comosus L.)

Unavailability of performant planting material of pineapple constitutes a major problem of its cultivation in Africa. For this purpose, indirect organogenesis technique is used to evaluate the in vitro responses of two cultivars of pineapple during the explant’s regeneration. Calli were induced from crown leaf and plantlets leaf of “Smooth Cayenne” and “Sugarloaf cultivars”. Murashige and Skoog medium with vitamins B5 supplemented with different growth regulators combinations were used. BAP and/or 2,4-D have been added to base medium for calli cells’ differentiation while BAP and GA3 have been added for plant elongation. The results indicated that explants from regenerated plantlets leaves cultivated on MS supplemented with copper (II) sulphate 5-hydrate concentrations incorporated had significant (p < 0.0001) influence on callus induction in pineapple cultivars. Likewise, MS medium with NAA (0.5 mg/l) + BAP (1 mg/l) had a highly significant influence with 8.8 differentiated Calli. Also, MS medium supplemented with BAP (3 mg/l) + GA3 (2 mg/l) for the “smooth Cayenne” had significantly influenced (p < 0.0001) Calli regeneration with a high rate of 55.25% plantlets. MS medium containing 0.5 mg/l of NAA + 0 mg/l IBA produced a high number of roots in Sugarloaf whereas the medium containing 1.5 mg/l NAA + 0.5 mg/l (IBA) produced high number of roots in smooth Cayenne. We have established an efficient and reproducible protocol for mass propagation and genetic transformation of pineapple though indirect organogenesis. This protocol may be used in genetics engineering studies for pineapple breeding.

An updated review of pineapple and its bioactive compounds in breast cancer

Women are more likely than men to develop cancer of the breast.Most breast cancer drugs are highly toxic,and treatment can cause side effects.It is imperative to find safe alternative medicines in the pursuit of a cure for breast cancer.An extract of pineapple contains cysteine proteases known as bromelains.In general,pineapples are regarded as safe foods.From the fruit,stem,and of pineapples,bromelain is produced by multiple endopeptidases.As well as reducing the growth of tumors locally,bromelain severely impaired the cytotoxicity of monocytes in the immune system in the fight against cancer.Specifically,we investigated pineapple’s possible mechanisms of action and its bioactive compounds in breast cancer.

菠萝果形态及力学特性试验研究

为减轻菠萝收获人工劳动强度,设计开发了机械化收获设备,并研究了我国菠萝主产区主栽品种菠萝果的形态及力学特性。确定了主栽品种菠萝果实形态参数,单果重分布在750~2500g,果实纵径分布在14.51~18.33cm;不同品种之间存在极显著差异,果实横径分布在10.22~13.24cm,品种之间差异不大。通过拉伸抗压试验及剪切强度试验,确定菠萝果实果肉抗压强度平均值为0.32MPa,弹性模量平均值为2.62MPa;果肉剪切强度平均值为0.16MPa,果芯抗压强度平均值为0.37MPa,弹性模量平均值为3.04MPa,果芯剪切强度平均值为0.25MPa。

考虑动态能耗的无人机采收菠萝田间收集点优化配置研究

为了深化无人机在田间菠萝采收和运输过程中的应用,探索无人机悬停放置菠萝到收集箱中的技术方案。无人机的续航能力限制菠萝采收工作的完成,而对续航能力影响最大的因素是无人机的载重,提出在田间设立菠萝收集点,并确定收集点的最优设立数量及位置,以确保菠萝采收工作的有效完成。在研究方法上,基于K-means算法寻找最佳收集点位置及分组方式,建立无人机采收菠萝的能耗及成本模型,通过编写程序对模型进行求解,并以无人机续航能力为约束条件,确定了无人机采收菠萝的最大覆盖范围和菠萝采摘数量。通过比较不同设立数量和位置的收集点方案,得出综合最优的结果。在不更换无人机电池的情况下,1台农业无人机采收菠萝约480个,采收面积约44m^(2)。随着收集点设立数量增加,无人机总路程和总能耗呈稳步下降状态,总成本呈稳步上升状态,分析发现667 m^(2)菠萝地设立15个固定位置的收集点时总能耗、总路程和总成本达到均衡最优。无人机采收菠萝的收集点配置优化方案,可为应用无人机进行田间作物采收及运输时收集点的数量和选址提供建设性意见。

菠萝抗寒研究进展

低温是限制菠萝产量和品质的重要因素之一。我国菠萝种植适宜区冬季周期性地受到低温等自然灾害影响,且近年来频繁遭遇严峻气候环境挑战,造成减产或失收,严重影响菠萝产业可持续发展。目前,关于菠萝抗寒性研究主要集中于抗寒种质资源鉴定与评价、抗寒性评价方法、生理生化测定、抗寒基因挖掘和抗寒防治措施等方面,而在菠萝抗寒性分子机制方面的研究进展较缓慢,本文针对这些方面进行概述,以期为菠萝抗寒性状的遗传改良及生产上防寒防冻提供参考。

菠萝果脯原料的优化选择

为获得优质的菠萝果脯,对菠萝原料进行选择。本实验以西双版纳不同成熟度的菠萝为原料,根据外观、颜色、香气、果实硬度区分菠萝成熟度之后,进行感官评价,同时测定水分、糖、酸、蛋白质等指标,然后进行菠萝原料与菠萝果脯的相关性分析,根据菠萝原料的关键指标和感官品评评价果脯的品质。结果表明,根据菠萝的口感、颜色、香气、果肉硬度将菠萝成熟度划分为轻熟、中熟、全熟,在菠萝鲜果的感官评价体系中中熟菠萝色泽、口感、香气、果肉硬度四项指标得分均为最高,且综合得分也高于轻熟菠萝和全熟菠萝,因此最终确定中熟的菠萝鲜果最适宜作为果脯原料。在对不同采样期中熟菠萝指标进行分析时发现,不同采样期菠萝含糖量变化波动较大,与气候状况有直接关系;酸含量伴随菠萝成熟过程先升高再降低,最后略微升高;水分则与采样期天气状况密切相关;蛋白质随着采样期延长营养物质积累而升高。由菠萝原料与菠萝果脯的相关性可知,菠萝原料的糖、酸、蛋白质含量会给菠萝果脯的品质带来影响。菠萝原料的糖、酸、水分、蛋白质与菠萝果脯品质具有密切关系,由此可根据菠萝原料的相关指标对菠萝果脯品质进行预测,也能筛选出适合制作菠萝果脯的原料,为制作优质菠萝果脯提供依据,并可进一步改善菠萝果脯的综合品质。

菠萝果渣中不溶性膳食纤维提取工艺优化及其功能特性研究

以菠萝果渣为原料,采用碱处理法提取菠萝不溶性膳食纤维(PIDF)。以PIDF得率为指标,在单因素试验基础上通过响应面法优化提取工艺,并探究PIDF的结构和功能特性。结果表明:最优工艺参数为液料比26∶1(mL/g)、NaOH体积分数6%、提取温度40℃、提取时间75 min,在此条件下PIDF得率为48.81%;PIDF具有疏松的孔洞结构,其持水性、持油性和膨胀性分别为(5.16±0.21)g/g、(3.86±0.11)g/g和(5.49±0.09)mL/g,表明PIDF具有较好的结构及功能特性。

改性菠萝皮对水中亚甲基蓝的吸附性能研究

以农业废弃物菠萝皮为原料,使用柠檬酸对菠萝皮粉末分别进行超声波改性和热改性,探究了改性菠萝皮对亚甲基蓝的去除效果。通过扫描电子显微镜(SEM)和傅里叶变换红外光谱(FTIR)对改性前后菠萝皮孔隙和表面结构进行表征,同时考察了改性前后菠萝皮的投加量、pH值、反应时间和染料质量浓度对吸附亚甲基蓝的影响。结果表明:经过柠檬酸改性后的菠萝皮表面引入了羧基官能团,对亚甲基蓝的吸附效果显著增加。在吸附剂用量为1.2 g·L^(-1)、起始p H为6.52、改性时间90 min、起始质量浓度为100 mg·L^(-1)的条件下,柠檬酸超声波改性菠萝皮(PA-US)和柠檬酸热改性菠萝皮(PA-TM)对亚甲基蓝的去除率分别可以达到65%、98%。吸附等温线的研究表明,PA-US和PA-TM吸附过程均符合Langmuir等温模型,说明该吸附过程为单分子层吸附,且对亚甲基蓝的最大吸附量分别为56.05 mg·g^(-1)和333.33 mg·g^(-1),与未改性菠萝皮(PA)的最大吸附量相比,分别提高了1.39、8.08倍。吸附动力学研究表明,PA-US和PA-TM对亚甲基蓝的吸附均符合准二级动力学方程模型。由吸附等温线和动力学模型结果可以推断该吸附过程属于化学吸附。

乙烯利对5个菠萝品种成花及品质的影响

【目的】筛选出不同菠萝品种适宜的乙烯利催花质量浓度,为菠萝成熟期调节及新品种推广提供参考依据。【方法】利用25~1000 mg·L^(-1)乙烯利对Josapine、台农4号、MD-2、台农21号和台农22号5个菠萝品种进行灌心处理,探究不同乙烯利质量浓度对各菠萝品种成花率、抽蕾期、果实内外品质及畸形率的影响。【结果】除台农22号外,各菠萝品种随着乙烯利质量浓度的增加成花率显著提升。其中,Josapine和台农4号诱导成花的最佳质量浓度为400 mg·L^(-1),MD-2和台农21号诱导成花的最佳质量浓度为800 mg·L^(-1)。当处理质量浓度大于400 mg·L^(-1)时,Josapine、台农4号和台农21号抽蕾期进一步缩短,MD-2抽蕾期则逐渐延长。当乙烯利质量浓度大于200 mg·L^(-1)时,Josapine、MD-2和台农22号纵横径、单果质量等形态指标呈下降趋势。随着乙烯利质量浓度的升高,各品种可滴定酸含量呈下降趋势;相反地,可溶性固形物含量随质量浓度的升高呈上升的趋势。此外,5个菠萝品种在乙烯利诱导下均有畸形果产生,其中Josapine在高质量浓度乙烯利作用下,畸形率最高,达到65.52%,而MD-2畸形率仅为6.67%。【结论】Josapine最适乙烯利催花质量浓度为400 mg·L^(-1);台农4号、MD-2和台农21号最适质量浓度为800 mg·L^(-1);而单一乙烯利不能诱导台农22号成花。

干酪乳杆菌LK-1的发酵特性及其在菠萝果汁中的应用

为利用干酪乳杆菌制备发酵菠萝果汁饮品,本研究对干酪乳杆菌LK-1(Lactobacillus casei LK-1,L. casei LK-1)的生长曲线、产酸、耐酸和耐糖等特性进行研究分析,并以活菌数和总酸为指标,通过单因素实验及响应面法考察L. casei LK-1发酵菠萝果汁的最佳条件。结果表明,L. casei LK-1在pH5~7和0%~10%质量分数葡萄糖环境下,具备良好的生长繁殖能力;在酸性(pH3)和高糖(40%质量分数葡萄糖)环境中培养3 h,其存活率分别为76%和71%;在MRS培养基中培养48 h,产酸量为5.35 g/kg,产酸能力良好,适用于果汁的发酵。L. casei LK-1发酵菠萝果汁的最佳条件为:初始pH6.8,发酵温度37℃,接种量1%(v/v),发酵时间30 h,此条件下制备的发酵菠萝果汁饮品活菌数为8.99±0.04 lg CFU/mL,总酸含量为7.16±0.26 g/kg,且色泽鲜亮,酸甜适口,风味较好,为L. casei LK-1在发酵食品中的进一步应用提供了理论和技术依据。

基于机器视觉的菠萝自动去眼方法与试验

菠萝加工过程中的去眼作业目前主要依赖人工手动操作,劳动成本高且作业效率低。为实现自动化菠萝去眼作业,该研究运用YOLOv5目标检测算法对菠萝眼进行快速识别,将所有角度相差90o的两张图片作为一组进行立体匹配分析以获取菠萝眼的三维位置信息。通过旋转菠萝和轴向移动去眼刀具将专用去眼刀具依次对准每一个菠萝眼并快速去除。试验结果表明:YOLOv5目标检测算法对菠萝眼识别效果良好,验证集的准确率、召回率和平均精度均值均高于96%;菠萝眼实际中心与探针刺入位置的平均误差为1.01 mm,最大误差为2.17 mm,均方根误差为1.09 mm;菠萝眼的完全去除率为89.5%、不完全去除率为6.2%,漏检率为4.3%,单个菠萝去眼时间为110.9 s,基本满足自动化去眼作业需要。研究结果可为菠萝自动去眼机研发提供技术参考。