Background:To investigate the effects of acupuncture on post-stroke limb spasm model rats and the underlying mechanism.Methods:A total of 50 Sprague-Dawley rats were randomly divided into three groups,Control group(10...Background:To investigate the effects of acupuncture on post-stroke limb spasm model rats and the underlying mechanism.Methods:A total of 50 Sprague-Dawley rats were randomly divided into three groups,Control group(10),Model group(20)and Zhishen Tiaoxing(ZSTX)acupuncture group(20).Middle cerebral artery occlusion was conducted in SD rats to establish post-stroke limb spasm rats,which were treated with ZSTX acupuncture.Behavioral assays were determined by the Narrow ally test,the limb muscle tension was detected by the BL-420S test system,and infarct volume was assessed after the cerebral infarction by 2,3,5-triphenyltetrazolium chloride staining.Heterogeneous neurotransmitterγ-aminobutyric acid(GABA)and its receptors GABAA and GABAB in the cerebral cortex of the infarct area were determined by immunofluorescence assay.The release of Trkb and K-Cl cotransporter isoform 2 was detected by an immunofluorescence double labeling study.Western Blot was utilized to measure the expression of BDNF and Trkb.Results:The results showed that the behavioral assays in post-stroke limb spasm rats were significantly improved by the treatment of ZSTX acupuncture.14 days of ZSTX acupuncture can effectively inhibit muscle tone and decrease Infarct volume,which was measured with BL-420S biological function experiment system and triphenyltetrazolium chloride.Meanwhile,the results of Double-Label Immunofluorescence Assays showed that ZSTX acupuncture improved the expression of GABA,GABAA,GABAB,BDNF,and K-Cl cotransporter isoform 2.Double-Label Immunofluorescence Assays and WB results showed that 14 days ZSTX acupuncture declined the expression of Trkb.Conclusions:Our results suggest that 14 days of ZSTX acupuncture can significantly improve post-stroke limb spasm.Meanwhile,the pathogenesis of post-stroke limb spasm and the efficacy of ZSTX acupuncture involve metabolic pathways of neurotransmitters,and electro-acupuncture can treat post-stroke limb spasm by regulating BDNF/Trkb-KCC2 signaling pathway.展开更多
Objective: To describe the latest progress in the use of botulinum neurotoxin for post-stroke limb spasm. Methods: This paper looks up the relevant research literatures in recent years in PubMed, Web of Science, Sprin...Objective: To describe the latest progress in the use of botulinum neurotoxin for post-stroke limb spasm. Methods: This paper looks up the relevant research literatures in recent years in PubMed, Web of Science, Springer, Ovid, CNKI, WanFang databases and summarizes them. Results: The latest progress in the use of botulinum neurotoxin for post-stroke limb spasm was studied from the following aspects: the action mechanism of botulinum neurotoxin;efficacy evaluation;injection dose;target muscle selection;guiding technology;combination therapy. Conclusion: Botulinum neurotoxin is the first-line treatment for post-stroke limb spasm. We need to make continuous improvement and progress from the treatment period, injection dose, target muscle selection, guiding technology and efficacy evaluation to improve the quality of life of the majority of post-stroke survivors in China.展开更多
目的观察针刺联合冲击波治疗对卒中后上肢功能障碍的影响。方法选取90例卒中后上肢功能障碍患者作为研究对象,用随机数字表法分成对照组、针刺组和联合组,每组30例。对照组行常规康复训练和西医治疗,针刺组在对照组治疗基础上予针刺治疗...目的观察针刺联合冲击波治疗对卒中后上肢功能障碍的影响。方法选取90例卒中后上肢功能障碍患者作为研究对象,用随机数字表法分成对照组、针刺组和联合组,每组30例。对照组行常规康复训练和西医治疗,针刺组在对照组治疗基础上予针刺治疗,联合组在针刺组治疗基础上予冲击波治疗。比较3组治疗前、治疗2周后及治疗4周后上肢Fugl-Meyer运动功能量表(Fugl-Meyer assessment,FMA)、运动功能状态量表(motor status scale,MSS)、上肢Wolf运动功能测试量表(Wolf motor function test,WMFT)和日常生活活动(activities of daily living,ADL)的评分变化。比较3组治疗前后上肢改良Ashworth量表(modified Ashworth scale,MAS)评分的等级。结果与对照组比较,针刺组治疗2周后和治疗4周后上肢FMA评分、MSS评分、WMFT评分和ADL评分均升高(P<0.05);与针刺组比较,联合组治疗2周后和治疗4周后上肢FMA评分、MSS评分、WMFT评分和ADL评分均升高(P<0.05)。与对照组比较,针刺组治疗后痉挛等级低于2级的患者占比升高(P<0.05);与针刺组比较,联合组治疗后痉挛等级低于2级的患者占比升高(P<0.05)。结论在常规康复训练和西医治疗基础上,针刺联合冲击波治疗能有效促进卒中患者上肢功能的恢复,改善患者上肢屈肌痉挛,提高患者日常生活活动能力。展开更多
目的观察重复性外周磁刺激(repetitive peripheral magnetic stimulation,rPMS)联合常规治疗对脑卒中患者瘫痪侧上肢屈肘肌痉挛的影响。方法选取2019年9月至2020年9月在泰州市人民医院康复医学科接受康复治疗的脑卒中瘫痪上肢屈肘肌痉...目的观察重复性外周磁刺激(repetitive peripheral magnetic stimulation,rPMS)联合常规治疗对脑卒中患者瘫痪侧上肢屈肘肌痉挛的影响。方法选取2019年9月至2020年9月在泰州市人民医院康复医学科接受康复治疗的脑卒中瘫痪上肢屈肘肌痉挛患者60例,随机分为实验组及对照组各30例。两组患者均给予常规治疗,实验组患者在此基础上给予重复性外周磁刺激治疗,对照组则给予假磁刺激治疗。在治疗前和治疗4周后选用改良Ashworth量表(modified Ashworth scale,MAS)对肌张力分级进行评估,选用表面肌电仪检测肱二头肌肌电积分值(iEMG)对痉挛情况进行判断,选用Fugl-meyer量表上肢部分(Fugl-Meyer motor assessment scale of Upper Extremity,FMA-UE)对患者上肢运动功能情况进行评估,选用改良Barthel指数(MBI)对患者日常生活活动能力情况进行评估。结果治疗4周后,组内比较,实验组及对照组患者Ashworth分级均较治疗前下降(P<0.05);两组患者肱二头肌iEMG值均较治疗前降低(P<0.05);两组患者FMA-UE评分、MBI评分均较治疗前升高(P<0.05);组间比较,实验组治疗后的上述疗效指标改善情况均较对照组更为显著,组间差异均具有统计学意义(P<0.05)。结论重复性外周磁刺激联合常规治疗可以更有效地减轻脑卒中后瘫痪肢体上肢屈肘肌痉挛,并且提高患者上肢运动功能及日常生活活动能力。展开更多
基金the National Key R&D Program of China(Grant No.2019YFC1709900)National Natural Science Foundation of China(Grant No.71804022)+1 种基金Natural Science Foundation of Henan province(Grant No.232300420256)Medical technologies R&D Program of Henan province(Grant No.LHGJ20220348).
文摘Background:To investigate the effects of acupuncture on post-stroke limb spasm model rats and the underlying mechanism.Methods:A total of 50 Sprague-Dawley rats were randomly divided into three groups,Control group(10),Model group(20)and Zhishen Tiaoxing(ZSTX)acupuncture group(20).Middle cerebral artery occlusion was conducted in SD rats to establish post-stroke limb spasm rats,which were treated with ZSTX acupuncture.Behavioral assays were determined by the Narrow ally test,the limb muscle tension was detected by the BL-420S test system,and infarct volume was assessed after the cerebral infarction by 2,3,5-triphenyltetrazolium chloride staining.Heterogeneous neurotransmitterγ-aminobutyric acid(GABA)and its receptors GABAA and GABAB in the cerebral cortex of the infarct area were determined by immunofluorescence assay.The release of Trkb and K-Cl cotransporter isoform 2 was detected by an immunofluorescence double labeling study.Western Blot was utilized to measure the expression of BDNF and Trkb.Results:The results showed that the behavioral assays in post-stroke limb spasm rats were significantly improved by the treatment of ZSTX acupuncture.14 days of ZSTX acupuncture can effectively inhibit muscle tone and decrease Infarct volume,which was measured with BL-420S biological function experiment system and triphenyltetrazolium chloride.Meanwhile,the results of Double-Label Immunofluorescence Assays showed that ZSTX acupuncture improved the expression of GABA,GABAA,GABAB,BDNF,and K-Cl cotransporter isoform 2.Double-Label Immunofluorescence Assays and WB results showed that 14 days ZSTX acupuncture declined the expression of Trkb.Conclusions:Our results suggest that 14 days of ZSTX acupuncture can significantly improve post-stroke limb spasm.Meanwhile,the pathogenesis of post-stroke limb spasm and the efficacy of ZSTX acupuncture involve metabolic pathways of neurotransmitters,and electro-acupuncture can treat post-stroke limb spasm by regulating BDNF/Trkb-KCC2 signaling pathway.
文摘Objective: To describe the latest progress in the use of botulinum neurotoxin for post-stroke limb spasm. Methods: This paper looks up the relevant research literatures in recent years in PubMed, Web of Science, Springer, Ovid, CNKI, WanFang databases and summarizes them. Results: The latest progress in the use of botulinum neurotoxin for post-stroke limb spasm was studied from the following aspects: the action mechanism of botulinum neurotoxin;efficacy evaluation;injection dose;target muscle selection;guiding technology;combination therapy. Conclusion: Botulinum neurotoxin is the first-line treatment for post-stroke limb spasm. We need to make continuous improvement and progress from the treatment period, injection dose, target muscle selection, guiding technology and efficacy evaluation to improve the quality of life of the majority of post-stroke survivors in China.
文摘目的观察针刺联合冲击波治疗对卒中后上肢功能障碍的影响。方法选取90例卒中后上肢功能障碍患者作为研究对象,用随机数字表法分成对照组、针刺组和联合组,每组30例。对照组行常规康复训练和西医治疗,针刺组在对照组治疗基础上予针刺治疗,联合组在针刺组治疗基础上予冲击波治疗。比较3组治疗前、治疗2周后及治疗4周后上肢Fugl-Meyer运动功能量表(Fugl-Meyer assessment,FMA)、运动功能状态量表(motor status scale,MSS)、上肢Wolf运动功能测试量表(Wolf motor function test,WMFT)和日常生活活动(activities of daily living,ADL)的评分变化。比较3组治疗前后上肢改良Ashworth量表(modified Ashworth scale,MAS)评分的等级。结果与对照组比较,针刺组治疗2周后和治疗4周后上肢FMA评分、MSS评分、WMFT评分和ADL评分均升高(P<0.05);与针刺组比较,联合组治疗2周后和治疗4周后上肢FMA评分、MSS评分、WMFT评分和ADL评分均升高(P<0.05)。与对照组比较,针刺组治疗后痉挛等级低于2级的患者占比升高(P<0.05);与针刺组比较,联合组治疗后痉挛等级低于2级的患者占比升高(P<0.05)。结论在常规康复训练和西医治疗基础上,针刺联合冲击波治疗能有效促进卒中患者上肢功能的恢复,改善患者上肢屈肌痉挛,提高患者日常生活活动能力。
文摘目的观察重复性外周磁刺激(repetitive peripheral magnetic stimulation,rPMS)联合常规治疗对脑卒中患者瘫痪侧上肢屈肘肌痉挛的影响。方法选取2019年9月至2020年9月在泰州市人民医院康复医学科接受康复治疗的脑卒中瘫痪上肢屈肘肌痉挛患者60例,随机分为实验组及对照组各30例。两组患者均给予常规治疗,实验组患者在此基础上给予重复性外周磁刺激治疗,对照组则给予假磁刺激治疗。在治疗前和治疗4周后选用改良Ashworth量表(modified Ashworth scale,MAS)对肌张力分级进行评估,选用表面肌电仪检测肱二头肌肌电积分值(iEMG)对痉挛情况进行判断,选用Fugl-meyer量表上肢部分(Fugl-Meyer motor assessment scale of Upper Extremity,FMA-UE)对患者上肢运动功能情况进行评估,选用改良Barthel指数(MBI)对患者日常生活活动能力情况进行评估。结果治疗4周后,组内比较,实验组及对照组患者Ashworth分级均较治疗前下降(P<0.05);两组患者肱二头肌iEMG值均较治疗前降低(P<0.05);两组患者FMA-UE评分、MBI评分均较治疗前升高(P<0.05);组间比较,实验组治疗后的上述疗效指标改善情况均较对照组更为显著,组间差异均具有统计学意义(P<0.05)。结论重复性外周磁刺激联合常规治疗可以更有效地减轻脑卒中后瘫痪肢体上肢屈肘肌痉挛,并且提高患者上肢运动功能及日常生活活动能力。
