Accurate determination of flushing time is crucial for maintaining sustainable production in fish culture zones (FCZs), as it represents the physical self-purification capability via tidal exchange with clean water ...Accurate determination of flushing time is crucial for maintaining sustainable production in fish culture zones (FCZs), as it represents the physical self-purification capability via tidal exchange with clean water in the outer sea. However, owing to the temporal and spatial complexity of the coastal flushing process, existing methods for determining flushing time may not be generally applicable. In this paper, a systematic method for determining the flushing time in FCZs is presented, in which bathymetry, runoff, tidal range and stratification are properly accounted for. We determine the flushing time via numerical tracer experiments, using robust 3D hydrodynamic and mass transport models. For FCZs located in sheltered and land-locked tidal inlets, the system boundary can be naturally defined at the connection with the open sea. For FCZs located in open'waters, hydrodynamic tracking is first used to assess the extent of tidal excursion and thus delimit the initial boundary between clean water and polluted water. This general method is applied to all designated marine FCZs in Hong Kong for both the dry and wet seasons, including 20 sheltered FCZs (in semi-enclosed waters of Tolo Harbour, Mirs Bay, and Port Shelter) and 6 FCZs in open waters. Our results show that flushing time is the longest in inner Port Shelter (about 40 days in dry season), and the shortest for the FCZs in open waters (less than one week in dry season). In addition, the flushing time in dry season is commonly longer than that in wet season: 20%~40% for most well-sheltered FCZs; 2.6-4 times for the others. Our results indicate a positive correlation between the flushing time and distance to open boundary, supporting the view that the flushing time of a FCZ is closely related to its location. This study provides a solid basis for mariculture management such as the determination of carrying capacity of FCZs.展开更多
Freshly harvested paddy rice was randomly obtained from three different farms in “Ofada” town, Ogun State, Nigeria and processed according to the traditional parboiling method. The rice was inoculated singly with cu...Freshly harvested paddy rice was randomly obtained from three different farms in “Ofada” town, Ogun State, Nigeria and processed according to the traditional parboiling method. The rice was inoculated singly with cultures of Lactobacillus amylophilus, Leuconostoc mesenteroides, Saccharomyces uvarum and Saccharomyces cerevisiae which were isolated from the soak-water. In order to assess the effect of starter cultures on the soaking time of the rice, the pH and titratable acidity were determined at 12 h interval. The chemical composition and the sensory quality of the rice were also determined at the end of the soaking period while uninoculated rice served as control. The chemical composition of the rice as well as the pH and TTA of the soak-water were significantly (p < 0.05) different from the control while the processing time was shortened to 48 h. The rice inoculated with Saccharomyces uvarum and Saccharomyces cerevisae had the highest protein content and best sensory attributes.展开更多
Objective:To evaluate the impact of prolonged post-thaw embryos culture on pregnancy outcome during frozen embryo transfer cycles.Methods:This prospective cohort study evaluated 324 thaw transfer cycles with 819 embry...Objective:To evaluate the impact of prolonged post-thaw embryos culture on pregnancy outcome during frozen embryo transfer cycles.Methods:This prospective cohort study evaluated 324 thaw transfer cycles with 819 embryos from 269 patients at the Center for Reproductive Endocrinology and Infertility of Hue University Hospital in Vietnam.These frozen embryo transfer cycles were divided into two groups at the time of thawing:the short culture group(2-hour post-thaw culture)and the overnight culture group(overnight culture for 18 h)before the embryo was transferred into the uterus.The rates of embryo intact,grade A embryo at frozen and transfer time and continuing cleavage were recorded.The clinical outcomes including serum beta-human chorionic gonadotropin,clinical pregnancy and implantation rate were evaluated after 14 days,4 weeks,6 weeks,respectively,after embryo transfer.Results:Human chorionic gonadotropin positive occurred in 39.5%of patients in the short culture group compared to 25.9%in the overnight culture group with risk difference(RD)=13.6%,relative risk(RR)=1.343,95%confidence interval(CI)1.085-1.663,P<0.01.Clinical pregnancy rate of the short culture group and overnight culture group was 33.3%and 24.1%,respectively(RD=9.2%,RR=1.242,95%CI 0.996-1.549,P=0.06)and the implantation rate in the short culture group and overnight culture group was 16.5%and 11.0%,respectively(RD=5.5%,RR=1.244,95%CI 1.046-1.479,P=0.01).In women of advanced age(≥35 years)and women who received 3 embryos,pregnancy outcomes were found to be significantly(P<0.05)higher in the short culture than in the overnight culture group.Conclusions:The prolonged post-thaw culture period does not increase pregnancy outcome in comparison with the short culture.展开更多
<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. ...<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning </span><span lang="EN-US" style="font-family:;" "="">the </span><span lang="EN-US" style="font-family:;" "="">reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different <i>in vitro </i>culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant</span><span lang="EN-US" style="font-family:;" "="">,</span><span lang="EN-US" style="font-family:;" "=""> and anestrus stages, respectively, but did not show a significant difference (<i>P</i></span><span lang="EN-US" style="font-family:;" "=""> ></span><span lang="EN-US"> </span><span lang="EN-US" style="font-family:;" "="">0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (<i>P</i> > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (<i>P</i> < 0.05). In contrast, the aforementioned </span><span lang="EN-US" style="font-family:;" "="">result </span><span lang="EN-US" style="font-family:;" "="">was not observed in the pregnant and anestrus stages (<i>P</i> > 0.05), indicating that the <i>in vitro </i>culture duration is an important factor during <i>in vitro</i> maturation of domestic cat oocytes.</span>展开更多
译林版小学英语五、六年级教材中Culture time 板块的设置旨在引导学生在接触和了解英语国家文化的过程中更好地理解和使用英语,有利于培养学生的国际文化意识。基于Culture time的教学现象分析,笔者从关注教学方法的趣味性、关注与单...译林版小学英语五、六年级教材中Culture time 板块的设置旨在引导学生在接触和了解英语国家文化的过程中更好地理解和使用英语,有利于培养学生的国际文化意识。基于Culture time的教学现象分析,笔者从关注教学方法的趣味性、关注与单元话题及其他板块的联系、关注文化教学的适度拓展、关注教材资源的整合四个方面来提出优化Culture time教学的策略。展开更多
Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resi...Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less than 24 hours—something urine culture alone could not achieve. The relative ease of automating urine PCR testing, combined with its diagnostic accuracy and rapid turnaround time, makes it a valuable addition to modern medical practice for the laboratory diagnosis of UTIs.展开更多
The perceptions of time are influenced by different cultural background. American understanding of time is typical of western countries. This paper introduces and explains American cultural pattern, thus avoiding from...The perceptions of time are influenced by different cultural background. American understanding of time is typical of western countries. This paper introduces and explains American cultural pattern, thus avoiding from happening unpleasant things in the process of cross-cultural communication.展开更多
基金supported by the Hong Kong Jockey Club Charities Trust (Project Waterman)in part by a grant from the University Grants Committee of the Hong Kong Special Administrative Region (HKSAR),China (Project No. AoE/P-04/04) to the Area of Excellence in Marine Environment Research and Innovative Technology (MERIT)+1 种基金The support from the National Science Fund for Distinguished Young Scholars (Grant No. 50925932)the National Natural Science Foundation of China (Grant No.41001348)
文摘Accurate determination of flushing time is crucial for maintaining sustainable production in fish culture zones (FCZs), as it represents the physical self-purification capability via tidal exchange with clean water in the outer sea. However, owing to the temporal and spatial complexity of the coastal flushing process, existing methods for determining flushing time may not be generally applicable. In this paper, a systematic method for determining the flushing time in FCZs is presented, in which bathymetry, runoff, tidal range and stratification are properly accounted for. We determine the flushing time via numerical tracer experiments, using robust 3D hydrodynamic and mass transport models. For FCZs located in sheltered and land-locked tidal inlets, the system boundary can be naturally defined at the connection with the open sea. For FCZs located in open'waters, hydrodynamic tracking is first used to assess the extent of tidal excursion and thus delimit the initial boundary between clean water and polluted water. This general method is applied to all designated marine FCZs in Hong Kong for both the dry and wet seasons, including 20 sheltered FCZs (in semi-enclosed waters of Tolo Harbour, Mirs Bay, and Port Shelter) and 6 FCZs in open waters. Our results show that flushing time is the longest in inner Port Shelter (about 40 days in dry season), and the shortest for the FCZs in open waters (less than one week in dry season). In addition, the flushing time in dry season is commonly longer than that in wet season: 20%~40% for most well-sheltered FCZs; 2.6-4 times for the others. Our results indicate a positive correlation between the flushing time and distance to open boundary, supporting the view that the flushing time of a FCZ is closely related to its location. This study provides a solid basis for mariculture management such as the determination of carrying capacity of FCZs.
文摘Freshly harvested paddy rice was randomly obtained from three different farms in “Ofada” town, Ogun State, Nigeria and processed according to the traditional parboiling method. The rice was inoculated singly with cultures of Lactobacillus amylophilus, Leuconostoc mesenteroides, Saccharomyces uvarum and Saccharomyces cerevisiae which were isolated from the soak-water. In order to assess the effect of starter cultures on the soaking time of the rice, the pH and titratable acidity were determined at 12 h interval. The chemical composition and the sensory quality of the rice were also determined at the end of the soaking period while uninoculated rice served as control. The chemical composition of the rice as well as the pH and TTA of the soak-water were significantly (p < 0.05) different from the control while the processing time was shortened to 48 h. The rice inoculated with Saccharomyces uvarum and Saccharomyces cerevisae had the highest protein content and best sensory attributes.
文摘Objective:To evaluate the impact of prolonged post-thaw embryos culture on pregnancy outcome during frozen embryo transfer cycles.Methods:This prospective cohort study evaluated 324 thaw transfer cycles with 819 embryos from 269 patients at the Center for Reproductive Endocrinology and Infertility of Hue University Hospital in Vietnam.These frozen embryo transfer cycles were divided into two groups at the time of thawing:the short culture group(2-hour post-thaw culture)and the overnight culture group(overnight culture for 18 h)before the embryo was transferred into the uterus.The rates of embryo intact,grade A embryo at frozen and transfer time and continuing cleavage were recorded.The clinical outcomes including serum beta-human chorionic gonadotropin,clinical pregnancy and implantation rate were evaluated after 14 days,4 weeks,6 weeks,respectively,after embryo transfer.Results:Human chorionic gonadotropin positive occurred in 39.5%of patients in the short culture group compared to 25.9%in the overnight culture group with risk difference(RD)=13.6%,relative risk(RR)=1.343,95%confidence interval(CI)1.085-1.663,P<0.01.Clinical pregnancy rate of the short culture group and overnight culture group was 33.3%and 24.1%,respectively(RD=9.2%,RR=1.242,95%CI 0.996-1.549,P=0.06)and the implantation rate in the short culture group and overnight culture group was 16.5%and 11.0%,respectively(RD=5.5%,RR=1.244,95%CI 1.046-1.479,P=0.01).In women of advanced age(≥35 years)and women who received 3 embryos,pregnancy outcomes were found to be significantly(P<0.05)higher in the short culture than in the overnight culture group.Conclusions:The prolonged post-thaw culture period does not increase pregnancy outcome in comparison with the short culture.
文摘<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning </span><span lang="EN-US" style="font-family:;" "="">the </span><span lang="EN-US" style="font-family:;" "="">reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different <i>in vitro </i>culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant</span><span lang="EN-US" style="font-family:;" "="">,</span><span lang="EN-US" style="font-family:;" "=""> and anestrus stages, respectively, but did not show a significant difference (<i>P</i></span><span lang="EN-US" style="font-family:;" "=""> ></span><span lang="EN-US"> </span><span lang="EN-US" style="font-family:;" "="">0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (<i>P</i> > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (<i>P</i> < 0.05). In contrast, the aforementioned </span><span lang="EN-US" style="font-family:;" "="">result </span><span lang="EN-US" style="font-family:;" "="">was not observed in the pregnant and anestrus stages (<i>P</i> > 0.05), indicating that the <i>in vitro </i>culture duration is an important factor during <i>in vitro</i> maturation of domestic cat oocytes.</span>
文摘译林版小学英语五、六年级教材中Culture time 板块的设置旨在引导学生在接触和了解英语国家文化的过程中更好地理解和使用英语,有利于培养学生的国际文化意识。基于Culture time的教学现象分析,笔者从关注教学方法的趣味性、关注与单元话题及其他板块的联系、关注文化教学的适度拓展、关注教材资源的整合四个方面来提出优化Culture time教学的策略。
文摘Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less than 24 hours—something urine culture alone could not achieve. The relative ease of automating urine PCR testing, combined with its diagnostic accuracy and rapid turnaround time, makes it a valuable addition to modern medical practice for the laboratory diagnosis of UTIs.
文摘The perceptions of time are influenced by different cultural background. American understanding of time is typical of western countries. This paper introduces and explains American cultural pattern, thus avoiding from happening unpleasant things in the process of cross-cultural communication.