Objective:To search the the differentially expressed genes between breast cell carcinoma tissues and normal tissues by using bioinformatics technology,and the potential therapeutic drugs for breast cancer were identif...Objective:To search the the differentially expressed genes between breast cell carcinoma tissues and normal tissues by using bioinformatics technology,and the potential therapeutic drugs for breast cancer were identified,which can provide reference for clinical immune targeted therapy and drug therapy of breast cancer in the future.Methods:"Breast cancer"was searched by using Gene Expression Omnibus(GEO),and GSE79586 chip data was downloaded.The differentially expressed genes in the control group and the breast cancer model group were screened by using bio-communication technology and subjected to GO function analysis,KEGG pathway analysis,differential gene characteristic expression analysis and protein-protein interaction network(PPI)analysis,and the analysis results were further visualized.Prognosis analysis,related function prediction and immune infiltration analysis were performed using the GEPIA,GeneMANIA,and Timer2.0 databases,respectively.Finally,the compounds with potential therapeutic effects on breast cancer are identified through Connectivity Map(CMap).Western blotting and real-time PCR(RT-PCR)were used to verify the core genes and potential therapeutic agents with the highest correlation in vitro.Results:A total of 3916 differentially expressed genes including 1786 up-regulated genes and 2130 down-regulated genes were screened.GO analysis showed that the differential genes were mainly involved in the positive regulation of phosphorylation,secretory vesicles,racemase and epimerase activities.KEGG analysis showed that differential genes were involved in systemic lupus erythematosus,alcoholism,sticky spots,amoebic dysentery Ras signal pathways and other disease pathways.The characteristic expression analysis of differential genes showed that MEK inhibitors,HSP90 inhibitors and signal transduction pathway kinase inhibitors were drugs similar to the differential genes.PPI results showed that H2AFJ,TFF1,GATA3,FOXA1,and CDH1 were core genes related to breast cancer.Two core genes of H2AFJ and TFF1 with the highest correlation were further selected for GEPIA analysis.The results of the analysis showed that the mRNA expression levels of H2AFJ and TFF1 in breast cancer cells were significantly higher than those in normal tissues,and there was a significant correlation with the pathological staging,overall survival rate and disease-free survival rate of breast cancer patients.H2AFJ and TFF1 may be potential prognostic biomarkers for survival of breast cancer patients.The functions of differentially expressed H2AFJ and TFF1 are mainly related to hormone receptor binding,epithelial structure maintenance and epigenetic negative regulation of genes,chromatin tissue involved in negative regulation of transcription,etc.The results of immune infiltration showed that the expressions of H2AFJ and TFF1 had a significant correlation with the infiltration of macrophages,neutrophils,monocytes,CD4+T,CD8+T,B lymphocytes and other immune cells.CMap results showed that compounds such as Gefitinib,Alpelisib,Sorafenib,and Sunitinib had potential therapeutic effects on breast cancer.Western blot and RT-PCR results showed that H2AFJ and TFF1 were significantly overexpressed in breast cancer cells.Gefitinib significantly inhibited the expression of H2AFJ and TFF1 in breast cancer cells(P<0.05,P<0.01).Conclusion:In this study,differentially expressed genes between breast cell carcinoma tissues and normal tissues were screened out by bioinformatics means to further identify key genes and compounds with potential therapeutic effects in the onset process of breast cancer and to further verify the effectiveness of the screened drugs on breast cancer through experiments.It will provide reference for clinical research and development of new drugs against breast cancer in the future in order to develop more effective treatment options.展开更多
Gd doped hollow nanoscale coordination polymers with multimodal imaging capabilities were synthesized by solvothermal method and further coated by silica layer.The in vitro tests demonstrated uncoated and silica-coate...Gd doped hollow nanoscale coordination polymers with multimodal imaging capabilities were synthesized by solvothermal method and further coated by silica layer.The in vitro tests demonstrated uncoated and silica-coated nanoprobes exhibit longitudinal relaxivities(r1)of 7.38 and 13.57(mmol/L)-1·s-1,and transverse relaxivities(r2)of 180.6 and 304.8(mmol/L)-1·s-1,showing fairly good dual T1&T2 contrast effects,and it also emits excellent multicolor uorescence under laser beams of various wavelengths.With the com-bination of magnetic resonance imaging(MRI)(both T1 and T2)and uorescence optical imaging(FOI),the nanoprobes could correlate preoperative diagnosis with intraoperative pathology.Furthermore,it also exhibits high drug loading capacity of 1166 mg/g and en-capsulation efficiency of 83.29%,which makes it a potential platform as drug carriers.The MTT assay demonstrates the moderate toxicity of the NPs,and after the silica coating process,not only the MRI contrast effects but also the biocompatibility have been enhanced.The versatility of the highly integrated systems can make up for the limitations of each imaging modality and exhibit great potentials for cancer theranostics.展开更多
The Editor welcomes submissions for possible publication in the Letters to the Editor section.Letters commenting on an article published in the Journal or other interesting pieces will be considered if they are receiv...The Editor welcomes submissions for possible publication in the Letters to the Editor section.Letters commenting on an article published in the Journal or other interesting pieces will be considered if they are received within 6 weeks of the time the article was published.Authors of the article being commented on will be given an opportunity to offer a timely response to the letter.Authors of letters will be notified that the letter has been received.Unpublished letters cannot be returned.展开更多
Natural product is one of the sources of drugs in pharmaceutical industry,and one of the notable origin of natural product is the medicinal plants.Medicinal plants tend to cure some certain diseases and could be a sou...Natural product is one of the sources of drugs in pharmaceutical industry,and one of the notable origin of natural product is the medicinal plants.Medicinal plants tend to cure some certain diseases and could be a source for potential drugs.The Department of Health of the Philippines approved 10 medicinal plants namely Allium sativum(Garlic/Bawang),Blumea balsamifera(Nagal camphor/sambong),Cassia alata(Ringworm bush/akapulko),Clinopodium douglasii(Mint/yerba Buena),Ehretia microphylla(Scorpion bush/Tsaang Gubat),Momordica charantia(Bitter Melon/Ampalaya),Peperomia pellucida(Silver bush/ulasimang Bato),Psidium guajava(Guava/Bayabas),Quisqualis indica(Rangoon creeper/niyug-niyogan),and Vitex negundo(Five-leaved Chaste Tree/lagundi).The review was conducted to show that these medicinal plants are capable in treating infections and some diseases.It was found that Allium sativum for the treatmet of wounds,hypertension and tootache;Blumea balsimifera is effective in diuretic treatment for hypertension;Cassia alata for the treatment of scabies,fungal infection,athlete’s foot,tinea flava,ringworm;Clinopodium douglasii for the treatment of muscle pain,arthritis,rheumatism,cough,headache;Ehretia microphylla for the treatment of diarrhea and stomachache;Momordica charantia for the treatment of diabetes mellitus;Peperomia pellucida for the treatment of gout and rheumatism;Psidium guajava for the treatment of wounds and diarrhea;Quisqualis indica for anti-helmintic medicine;and Vitex negundo for the treatment of cough,asthma,and fever.In conclusion,these 10 medicinal plants have natural products that can be used as source for potential drugs.However,there are still many species of ethnobotanical plants that are not yet investigated thoroughly and might be a source for potential drugs.Therefore,more investigations should be done in other species of plant,most especially for the plants with practical used.展开更多
COVID-19 pandemic has shown greater severity in people with co-morbidities;diabetes being the leading cause of higher mortality rates in COVID-19 patients. Besides compromised immunity, there are other factors that ma...COVID-19 pandemic has shown greater severity in people with co-morbidities;diabetes being the leading cause of higher mortality rates in COVID-19 patients. Besides compromised immunity, there are other factors that make diabetics more prone to SARS-CoV-2 infection. To date, there is no clinically proven treatment for this disease, but fortunately there are several reports of vaccines in different stages of development. This review compiles some commonly used anti-diabetic drugs and their probable efficacy during a COVID-19 attack. This is also an attempt to understand the cause of severity of SARS-CoV-2 infection in diabetic patients. Until a proper cure or approved vaccine is available, it is best to manage the disease by improving the immune status and making use of already available drugs that show potential against the virus.展开更多
基金supported by Supported by the National Natural Science Foundation of China(81603418,82074271)Scientific Research Project of"Outstanding Innovative Talents Support Plan"of Heilongjiang University of Chinese Medicine(2020YQ05)。
文摘Objective:To search the the differentially expressed genes between breast cell carcinoma tissues and normal tissues by using bioinformatics technology,and the potential therapeutic drugs for breast cancer were identified,which can provide reference for clinical immune targeted therapy and drug therapy of breast cancer in the future.Methods:"Breast cancer"was searched by using Gene Expression Omnibus(GEO),and GSE79586 chip data was downloaded.The differentially expressed genes in the control group and the breast cancer model group were screened by using bio-communication technology and subjected to GO function analysis,KEGG pathway analysis,differential gene characteristic expression analysis and protein-protein interaction network(PPI)analysis,and the analysis results were further visualized.Prognosis analysis,related function prediction and immune infiltration analysis were performed using the GEPIA,GeneMANIA,and Timer2.0 databases,respectively.Finally,the compounds with potential therapeutic effects on breast cancer are identified through Connectivity Map(CMap).Western blotting and real-time PCR(RT-PCR)were used to verify the core genes and potential therapeutic agents with the highest correlation in vitro.Results:A total of 3916 differentially expressed genes including 1786 up-regulated genes and 2130 down-regulated genes were screened.GO analysis showed that the differential genes were mainly involved in the positive regulation of phosphorylation,secretory vesicles,racemase and epimerase activities.KEGG analysis showed that differential genes were involved in systemic lupus erythematosus,alcoholism,sticky spots,amoebic dysentery Ras signal pathways and other disease pathways.The characteristic expression analysis of differential genes showed that MEK inhibitors,HSP90 inhibitors and signal transduction pathway kinase inhibitors were drugs similar to the differential genes.PPI results showed that H2AFJ,TFF1,GATA3,FOXA1,and CDH1 were core genes related to breast cancer.Two core genes of H2AFJ and TFF1 with the highest correlation were further selected for GEPIA analysis.The results of the analysis showed that the mRNA expression levels of H2AFJ and TFF1 in breast cancer cells were significantly higher than those in normal tissues,and there was a significant correlation with the pathological staging,overall survival rate and disease-free survival rate of breast cancer patients.H2AFJ and TFF1 may be potential prognostic biomarkers for survival of breast cancer patients.The functions of differentially expressed H2AFJ and TFF1 are mainly related to hormone receptor binding,epithelial structure maintenance and epigenetic negative regulation of genes,chromatin tissue involved in negative regulation of transcription,etc.The results of immune infiltration showed that the expressions of H2AFJ and TFF1 had a significant correlation with the infiltration of macrophages,neutrophils,monocytes,CD4+T,CD8+T,B lymphocytes and other immune cells.CMap results showed that compounds such as Gefitinib,Alpelisib,Sorafenib,and Sunitinib had potential therapeutic effects on breast cancer.Western blot and RT-PCR results showed that H2AFJ and TFF1 were significantly overexpressed in breast cancer cells.Gefitinib significantly inhibited the expression of H2AFJ and TFF1 in breast cancer cells(P<0.05,P<0.01).Conclusion:In this study,differentially expressed genes between breast cell carcinoma tissues and normal tissues were screened out by bioinformatics means to further identify key genes and compounds with potential therapeutic effects in the onset process of breast cancer and to further verify the effectiveness of the screened drugs on breast cancer through experiments.It will provide reference for clinical research and development of new drugs against breast cancer in the future in order to develop more effective treatment options.
基金supported by the National Natural Science Foundation of China (No.21571168)the Ministry of Science and Technology Grant (No.2016YFA0101202, No.U1232211,and No.31501130)CAS/SAFEA international partnership program for creative research teams and CAS Hefei Science Center (No.2016HSCIU011)
文摘Gd doped hollow nanoscale coordination polymers with multimodal imaging capabilities were synthesized by solvothermal method and further coated by silica layer.The in vitro tests demonstrated uncoated and silica-coated nanoprobes exhibit longitudinal relaxivities(r1)of 7.38 and 13.57(mmol/L)-1·s-1,and transverse relaxivities(r2)of 180.6 and 304.8(mmol/L)-1·s-1,showing fairly good dual T1&T2 contrast effects,and it also emits excellent multicolor uorescence under laser beams of various wavelengths.With the com-bination of magnetic resonance imaging(MRI)(both T1 and T2)and uorescence optical imaging(FOI),the nanoprobes could correlate preoperative diagnosis with intraoperative pathology.Furthermore,it also exhibits high drug loading capacity of 1166 mg/g and en-capsulation efficiency of 83.29%,which makes it a potential platform as drug carriers.The MTT assay demonstrates the moderate toxicity of the NPs,and after the silica coating process,not only the MRI contrast effects but also the biocompatibility have been enhanced.The versatility of the highly integrated systems can make up for the limitations of each imaging modality and exhibit great potentials for cancer theranostics.
文摘The Editor welcomes submissions for possible publication in the Letters to the Editor section.Letters commenting on an article published in the Journal or other interesting pieces will be considered if they are received within 6 weeks of the time the article was published.Authors of the article being commented on will be given an opportunity to offer a timely response to the letter.Authors of letters will be notified that the letter has been received.Unpublished letters cannot be returned.
文摘Natural product is one of the sources of drugs in pharmaceutical industry,and one of the notable origin of natural product is the medicinal plants.Medicinal plants tend to cure some certain diseases and could be a source for potential drugs.The Department of Health of the Philippines approved 10 medicinal plants namely Allium sativum(Garlic/Bawang),Blumea balsamifera(Nagal camphor/sambong),Cassia alata(Ringworm bush/akapulko),Clinopodium douglasii(Mint/yerba Buena),Ehretia microphylla(Scorpion bush/Tsaang Gubat),Momordica charantia(Bitter Melon/Ampalaya),Peperomia pellucida(Silver bush/ulasimang Bato),Psidium guajava(Guava/Bayabas),Quisqualis indica(Rangoon creeper/niyug-niyogan),and Vitex negundo(Five-leaved Chaste Tree/lagundi).The review was conducted to show that these medicinal plants are capable in treating infections and some diseases.It was found that Allium sativum for the treatmet of wounds,hypertension and tootache;Blumea balsimifera is effective in diuretic treatment for hypertension;Cassia alata for the treatment of scabies,fungal infection,athlete’s foot,tinea flava,ringworm;Clinopodium douglasii for the treatment of muscle pain,arthritis,rheumatism,cough,headache;Ehretia microphylla for the treatment of diarrhea and stomachache;Momordica charantia for the treatment of diabetes mellitus;Peperomia pellucida for the treatment of gout and rheumatism;Psidium guajava for the treatment of wounds and diarrhea;Quisqualis indica for anti-helmintic medicine;and Vitex negundo for the treatment of cough,asthma,and fever.In conclusion,these 10 medicinal plants have natural products that can be used as source for potential drugs.However,there are still many species of ethnobotanical plants that are not yet investigated thoroughly and might be a source for potential drugs.Therefore,more investigations should be done in other species of plant,most especially for the plants with practical used.
文摘COVID-19 pandemic has shown greater severity in people with co-morbidities;diabetes being the leading cause of higher mortality rates in COVID-19 patients. Besides compromised immunity, there are other factors that make diabetics more prone to SARS-CoV-2 infection. To date, there is no clinically proven treatment for this disease, but fortunately there are several reports of vaccines in different stages of development. This review compiles some commonly used anti-diabetic drugs and their probable efficacy during a COVID-19 attack. This is also an attempt to understand the cause of severity of SARS-CoV-2 infection in diabetic patients. Until a proper cure or approved vaccine is available, it is best to manage the disease by improving the immune status and making use of already available drugs that show potential against the virus.
