[Objectives] This study was conducted to observe the protective effect of Pratia extract on acute liver injury in mice.[Methods] Mice were randomly divided into 6 groups according to body weight,10 in each group: norm...[Objectives] This study was conducted to observe the protective effect of Pratia extract on acute liver injury in mice.[Methods] Mice were randomly divided into 6 groups according to body weight,10 in each group: normal control group,ethanol-induced/CCl4 liver injury model,low-dose,middle-dose and high-dose Pratia extract groups,and bifendatatum group.Except the blank group,other groups were given 50% ethanol intragastrically at a dose of 12 ml/kg to cause acute alcoholic liver injury,or intraperitoneally injected with 10% CCl4 soybean oil solution to cause acute CCl4 liver injury.The serum ALT and AST activity were measured as well as liver SOD and MDA concentrations.[Results] Pratia extract effectively reduced serum ALT and AST,decreased MDA content and increased liver tissue SOD activity.[Conclusions] Pratia extract has certain protective effect on acute liver injury.展开更多
[Objectives]This study was conducted to investigate the inhibitory effect of pratia extract on H22 tumor-bearing mice and the effects on immune organs.[Methods]With the application of H22 liver tumor-bearing mice as a...[Objectives]This study was conducted to investigate the inhibitory effect of pratia extract on H22 tumor-bearing mice and the effects on immune organs.[Methods]With the application of H22 liver tumor-bearing mice as an animal model,the animals were divided into such three Pratia extract groups as the high,medium and low dose groups(400,200 and 100 mg/kg)and cyclophosphamide CTX group(20 mg/kg).15 d after the administration,the animals were killed by cervical dislocation,and the tumors,thymuses and spleens were taken and weighed,followed by the calculation of the tumor inhibitory rate and the thymus and spleen index,and the serum tumor necrosis factor-α(TNF-α)and interleukin-2(IL-2)levels were determined by ELISA assay.[Results]The inhibitory rates were 54.1,32.6 and 8.2%,respectively,and there were significant differences from the model group(P<0.05);and the spleen index of the tumor-bearing mice was reduced,while the thymus index was improved.The serological results showed that the drug-administrated groups significantly improved the IL-2 levels in the tumor-bearing mice,but had no effects on TNF-α.[Conclusions]Pratia extract has an antitumor effect on H22 tumor-bearing mice,and show certain dose-effect relationship,and its mechanism may be related to enhancing the immune function in tumor-bearing mice by regulating IL-2.展开更多
[Objectives]This study was conducted to further reveal the chemical basis of the anti-tumor pharmacological activity of pratia.[Methods]The chemical composition of the ethyl acetate extract of pratia was systematicall...[Objectives]This study was conducted to further reveal the chemical basis of the anti-tumor pharmacological activity of pratia.[Methods]The chemical composition of the ethyl acetate extract of pratia was systematically studied by macroporous resin column chromatography,silica gel column chromatography and Sephadex LH-20 repeated silica gel column chromatography and spectroscopy.[Results]Eight compounds were separated from the ethyl acetate extract of pratia,among which six were identified,namely diosmin (1),diosmetin (2),linarin (3),quercetin (4),apigenin (5) and luteolin (6).All the compounds were separated from this plant for the first time.[Conclusions]This study provides reference for the identification of the physiological activity of pratia.展开更多
基金Supported by Youth Fund of Guangxi Natural Science Foundation(2010GXNSFB013075)Nanning Traditional Chinese Medicine Quality Control Engineering Research Center Construction Project(20181020-2)
文摘[Objectives] This study was conducted to observe the protective effect of Pratia extract on acute liver injury in mice.[Methods] Mice were randomly divided into 6 groups according to body weight,10 in each group: normal control group,ethanol-induced/CCl4 liver injury model,low-dose,middle-dose and high-dose Pratia extract groups,and bifendatatum group.Except the blank group,other groups were given 50% ethanol intragastrically at a dose of 12 ml/kg to cause acute alcoholic liver injury,or intraperitoneally injected with 10% CCl4 soybean oil solution to cause acute CCl4 liver injury.The serum ALT and AST activity were measured as well as liver SOD and MDA concentrations.[Results] Pratia extract effectively reduced serum ALT and AST,decreased MDA content and increased liver tissue SOD activity.[Conclusions] Pratia extract has certain protective effect on acute liver injury.
基金Supported by Youth Fund of Guangxi Natural Science Foundation(2010GXNSFB013075)
文摘[Objectives]This study was conducted to investigate the inhibitory effect of pratia extract on H22 tumor-bearing mice and the effects on immune organs.[Methods]With the application of H22 liver tumor-bearing mice as an animal model,the animals were divided into such three Pratia extract groups as the high,medium and low dose groups(400,200 and 100 mg/kg)and cyclophosphamide CTX group(20 mg/kg).15 d after the administration,the animals were killed by cervical dislocation,and the tumors,thymuses and spleens were taken and weighed,followed by the calculation of the tumor inhibitory rate and the thymus and spleen index,and the serum tumor necrosis factor-α(TNF-α)and interleukin-2(IL-2)levels were determined by ELISA assay.[Results]The inhibitory rates were 54.1,32.6 and 8.2%,respectively,and there were significant differences from the model group(P<0.05);and the spleen index of the tumor-bearing mice was reduced,while the thymus index was improved.The serological results showed that the drug-administrated groups significantly improved the IL-2 levels in the tumor-bearing mice,but had no effects on TNF-α.[Conclusions]Pratia extract has an antitumor effect on H22 tumor-bearing mice,and show certain dose-effect relationship,and its mechanism may be related to enhancing the immune function in tumor-bearing mice by regulating IL-2.
基金Supported by Youth Fund of Guangxi Natural Science Foundation(2010GXNSFB013075)
文摘[Objectives]This study was conducted to further reveal the chemical basis of the anti-tumor pharmacological activity of pratia.[Methods]The chemical composition of the ethyl acetate extract of pratia was systematically studied by macroporous resin column chromatography,silica gel column chromatography and Sephadex LH-20 repeated silica gel column chromatography and spectroscopy.[Results]Eight compounds were separated from the ethyl acetate extract of pratia,among which six were identified,namely diosmin (1),diosmetin (2),linarin (3),quercetin (4),apigenin (5) and luteolin (6).All the compounds were separated from this plant for the first time.[Conclusions]This study provides reference for the identification of the physiological activity of pratia.
