This work reports the mechanism of benzimidazoledithi(BDT) derivatives’ selective pre-enrichment of Cd2+under the stimulation of glutathione(GSH). The geometric and electronic properties of five BDT-M2+complexes(M = ...This work reports the mechanism of benzimidazoledithi(BDT) derivatives’ selective pre-enrichment of Cd2+under the stimulation of glutathione(GSH). The geometric and electronic properties of five BDT-M2+complexes(M = Cd, Cu, Hg, Pb, Co) have been investigated using density functional theory(DFT) at the B3 LYP/6-311 G(d,p) level with the GAUSSIAN 09 package program. The results show that BDT ligand exhibits alternative behaviors to different metal ions with the binding affinity in the order of Cu2+> Cd2+> Pb2+> Hg2+> Co2+. After adding the BDT-M2+complex into the GSH solution, the new S–S bonds can be formed, resulting with benzimidazole-metal ions(MBI-M2+) falling off into the GSH solution. Furthermore, the weak interaction between the new glutathione derivative(GSHD) and MBI-M2+were found. However, the strong chelation was detected between GSHD and MBI-M2+(M = Cu, Pb, Hg, Co) to hinder the adsorbed Cu2+, Pb2+and Hg2+, Co2+completely falling into the GSH solution, which suggests porous silicon composite modified by BDT has a certain selective pre-enrichment of Cd2+ion.展开更多
According to the FDA Bacteriological Analytical Manual (BAM) for Salmonella identification in produce, two pre-enrichment steps with 48 hours of incubation are the golden procedures. Lactose broth is recommended for t...According to the FDA Bacteriological Analytical Manual (BAM) for Salmonella identification in produce, two pre-enrichment steps with 48 hours of incubation are the golden procedures. Lactose broth is recommended for the first pre-enrichment step medium for leafy greens, and the universal pre-enrichment (UP) broth is for tomatoes. However, the suggested broths were evaluated to have the maximum performance using the culture-dependent methods, and may not be applied to other methods, such as biosensor detection platform. A wireless bacteriophage magnetoelastic (ME) biosensor has been recently developed for real-time or rapid detection of food-borne pathogens in various foods. This affinity-based biosensor utilizes a phage oligonucleotide as the probe to capture target bacteria. In this study, the efficiencies of different pre-enrichment media for early detection of low Salmonella on spinach leaves and tomatoes use ME biosensors to shorten detection time. Four broths of modified peptone water, Lennox broth (LB), lactose broth, and UP broth were selected in this study. Various pre-enrichment times for ME biosensor detection were investigated. After spiking 4 cfu/g Salmonella on the tomatoes surfaces, the phage biosensor was able to detect Salmonella within 5 hours of pre-enrichment comparing to 24 hours in the FDA procedures. For Salmonella spiked spinach leaves, the same medium showed Salmonella positive within 7 hours. This study demonstrated that LB broth is the best medium to shorten pre-enrichment time to pass Salmonella number detection thresholds for ME biosensor detection in spinach and tomatoes when comparing to FDA procedures.展开更多
基金supported by the National Natural Science Foundation of China(No.51504117,61764009,51762043)Yunnan Youth Fund Project(2016FD037)+1 种基金Talent Development Program of KUST(KKSY201563032)the Program for Innovative Research Team in University of Ministry of Education of China(No.IRT_17R48)
文摘This work reports the mechanism of benzimidazoledithi(BDT) derivatives’ selective pre-enrichment of Cd2+under the stimulation of glutathione(GSH). The geometric and electronic properties of five BDT-M2+complexes(M = Cd, Cu, Hg, Pb, Co) have been investigated using density functional theory(DFT) at the B3 LYP/6-311 G(d,p) level with the GAUSSIAN 09 package program. The results show that BDT ligand exhibits alternative behaviors to different metal ions with the binding affinity in the order of Cu2+> Cd2+> Pb2+> Hg2+> Co2+. After adding the BDT-M2+complex into the GSH solution, the new S–S bonds can be formed, resulting with benzimidazole-metal ions(MBI-M2+) falling off into the GSH solution. Furthermore, the weak interaction between the new glutathione derivative(GSHD) and MBI-M2+were found. However, the strong chelation was detected between GSHD and MBI-M2+(M = Cu, Pb, Hg, Co) to hinder the adsorbed Cu2+, Pb2+and Hg2+, Co2+completely falling into the GSH solution, which suggests porous silicon composite modified by BDT has a certain selective pre-enrichment of Cd2+ion.
文摘According to the FDA Bacteriological Analytical Manual (BAM) for Salmonella identification in produce, two pre-enrichment steps with 48 hours of incubation are the golden procedures. Lactose broth is recommended for the first pre-enrichment step medium for leafy greens, and the universal pre-enrichment (UP) broth is for tomatoes. However, the suggested broths were evaluated to have the maximum performance using the culture-dependent methods, and may not be applied to other methods, such as biosensor detection platform. A wireless bacteriophage magnetoelastic (ME) biosensor has been recently developed for real-time or rapid detection of food-borne pathogens in various foods. This affinity-based biosensor utilizes a phage oligonucleotide as the probe to capture target bacteria. In this study, the efficiencies of different pre-enrichment media for early detection of low Salmonella on spinach leaves and tomatoes use ME biosensors to shorten detection time. Four broths of modified peptone water, Lennox broth (LB), lactose broth, and UP broth were selected in this study. Various pre-enrichment times for ME biosensor detection were investigated. After spiking 4 cfu/g Salmonella on the tomatoes surfaces, the phage biosensor was able to detect Salmonella within 5 hours of pre-enrichment comparing to 24 hours in the FDA procedures. For Salmonella spiked spinach leaves, the same medium showed Salmonella positive within 7 hours. This study demonstrated that LB broth is the best medium to shorten pre-enrichment time to pass Salmonella number detection thresholds for ME biosensor detection in spinach and tomatoes when comparing to FDA procedures.