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HBV DNA高敏检测试剂盒的性能验证和临床应用评价 被引量:14
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作者 余学高 邓间开 +3 位作者 何小洪 陈培松 崔丹荔 黄彬 《分子诊断与治疗杂志》 2019年第2期111-116,共6页
目的对基于Pre-NAT全自动核酸提取系统的乙型肝炎病毒(hepatitis B virus,HBV)DNA高敏检测试剂盒(以下简称高敏试剂)进行性能验证和临床应用评价。方法根据美国临床实验室标准化协会(Clinical and Laboratory Standards Institute,CLSI... 目的对基于Pre-NAT全自动核酸提取系统的乙型肝炎病毒(hepatitis B virus,HBV)DNA高敏检测试剂盒(以下简称高敏试剂)进行性能验证和临床应用评价。方法根据美国临床实验室标准化协会(Clinical and Laboratory Standards Institute,CLSI)制定的试剂盒性能评价标准,采用高敏试剂分别对HBV标准品、临床HBV DNA高、中、低值样本及阴性样本进行定量检测,评价高敏试剂的精密度、正确度、分析测量范围、临床可报告范围和最低检测限等指标。同时采用高敏试剂和COBAS AmpliPrep/COBAS TaqMan 48高敏病毒载量系统检测44份乙型肝炎患者血清,进行方法学比较并分析高敏试剂与COBAS高敏检测系统的相关性。结果高敏试剂检测高、中、低值标本的批内不精密度s_r分别为0.069、0.085、0.059,室内不精密度s_l分别为0.083、30.138、0.117。偏倚标准差为0.25,小于厂家声明的偏倚标准差0.45。分析测量范围广,在25~(1.0×109)IU/mL范围内成线性。样本的最大稀释度为1∶80,功能灵敏度为10 IU/mL,临床可报告范围为10~(8.0×1010)IU/mL。最低检测限为10 IU/mL,低于厂家声明的检测下限20 IU/mL。高敏试剂与COBAS AmpliPrep/COBAS TaqMan 48病毒载量系统的相关性好(y=0.987 6 x+0.189 4,r=0.975 4,P<0.05)。结论所评价的高敏试剂的精密度和正确度高,分析测量范围和临床可报告范围宽,最低检测限低,与COBAS高敏检测系统的相关性好,具有良好的临床应用价值。 展开更多
关键词 HBV-DNA pre-nat系统 全自动核酸检测系统 性能验证
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Study on Feeding Effect of Different Levels of Protein and Energy on Production Performance of Pregnant Mother and Neo-Natal Calves
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作者 Nathu Ram Sarker Muhammad Khaiml Bashar Sheik Mohammad Jahangir Hossain Mohammed Khorshed Alam and Khan Shahidul Huque 《Journal of Agricultural Science and Technology(A)》 2015年第2期81-89,共9页
The present study was undertaken to assess feeding effect on productive and reproductive performances of pre-natal and post-natal Red Chittagong Cattle (RCC) cows under on-station condition. A total of 16 pregnant R... The present study was undertaken to assess feeding effect on productive and reproductive performances of pre-natal and post-natal Red Chittagong Cattle (RCC) cows under on-station condition. A total of 16 pregnant RCC cows having pregnancy 6-7 months and between 1-3 parities was selected for the study and was allocated randomly into four dietary treatment groups. There are four type diets, i.e., To (standard diet according to National Research Council (NRC), 1995), T1 (5% below standard diet), T2 (5% above standard diet), T3 (10% above standard diet) and a control treatment T4 (maintained as farm practice). The energy and protein requirements of experimental cows were determined as per standard developed by NRC. The results revealed that crude protein (CP) and metabolizable energy (ME, MJ/day) requirements among cows of different dietary groups were not significantly different, although CP requirements between T1 and T3 differed significantly. Total dry mater (DM) and ME intake differed significantly (P 〈 0.001) among cows of different dietary groups, but DM intake was significantly lower for cows in farm practice group Z4 compared to the cows of other dietary groups. The total CP intake had no significant variation (P 〉 0.05) among cows of all dietary groups, but CP intake from roughage varied significantly (P 〈 0.001). Final live weight and total live weight gain had no significant variation for cows of all groups, but there were significant (P 〈 0.05) variation for daily weight gain. Daily weight gain of cows for first four dietary groups were significantly (P 〈 0.05) higher than cows of farm practice groups T4. Weight of calf from birth to 90 days and daily weight gain calves under different dietary groups showed that there were no significant (P 〉 0.05) variation of calf birth weights for all dietary groups, although lower birth weight was found in farm practice groups T4. Gestation length and postpartum heat period of cows of different dietary treatment groups revealed that there was no significant (P 〉 0.05) variation for those traits among cows of different dietary treatment groups, although there seems to be shorter postpartum heat for cows in dietary group T2 and longer period in farm practice group T4. Total and daily milk yield up to 30 days and 90 days of cows had no significant (P 〉 0.05) effect for different dietary treatment groups, although there seems to be slightly better milk production performance for dietary T2 and To, respectively for 30 days and 90 days total and daily milk yield. There were no significant (P 〉 0.05) changes of total and daily weight gain of cows for all dietary groups, however cows of standard dietary group To performed slightly better than those of cows of other dietary groups. Milk composition of cows of different dietary treatment groups showed that there was no significant (P 〉 0.05) variation on milk composition for cows of different dietary groups. Post-natal body condition score (BCS) for the 1st, 3rd and 4th months differed significantly (P 〈 0.05) among different dietary groups, while not found significant difference on the 2nd month. Therefore, it may be concluded that pre-natal feeding has significant effect on body weight gain, birth weight of calves and milk production of dairy cows. Hence, it is suggested that better nutrition may be provided during pre-natal period to harvest good quality of calves and higher milk yield from post-natal period. 展开更多
关键词 pre-natal POST-NATAL postpartum heat period gestation length milk yield.
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