Endometrial cancer （EC） is the most common and lethal gynaecological cancer type in Europe and in North America. Frequently EC arises more in the corpus proper and manifests as round, polypoid expansile masses, but ...Endometrial cancer （EC） is the most common and lethal gynaecological cancer type in Europe and in North America. Frequently EC arises more in the corpus proper and manifests as round, polypoid expansile masses, but it may also originate in the lower uterine segment or spread in endometrium with necrosis and hemorrhage. The analysis was performed using a custom panel containing all DNA sequences loci coding pre-miRNAs and genes related to biogenesis and regulation of sncRNAs in normal and tumor tissues extracted from 6 unrelated patients with endometrial carcinoma. The identified variations were correlated with mature miRNAs differentially expressed in the same normal and tumor endometrial tissues. The comparison analysis confirmed the high degree of cellular and genetic intratumoral heterogeneity with a temporal and spatial miRNA expression distribution in association with genomic variants identified. The classification of specific DNA mutations, onto the loci identified, should be suitable to characterize possible instability genome regions and help classification of tumors to ameliorate the clinical management of patients affected by endometrial carcinoma.展开更多
MicroRNAs（miRNAs） are gene regulators involved in numerous diseases including cancer,heart disease,neurological disorders,vascular abnormalities and autoimmune conditions.Although hsa-mir-499 rs3746444 polymorphism ...MicroRNAs（miRNAs） are gene regulators involved in numerous diseases including cancer,heart disease,neurological disorders,vascular abnormalities and autoimmune conditions.Although hsa-mir-499 rs3746444 polymorphism was shown to contribute to the susceptibility of multiple genes to cancer,the data have yielded conflicting results.Therefore,this meta-analysis was performed to provide a comprehensive assessment of potential association between hsa-mir-499 rs3746444 polymorphism and cancer risk.In this meta-analysis,a total of 9 articles regarding 10 eligible case-control studies in English（including 6134 cases and 7141 controls） were analyzed.No significant association between hsa-mir-499 rs3746444 polymorphism and overall cancer risk was demonstrated.However,an increased risk was observed in the subgroup of breast cancer patients（G allele vs A allele：OR = 1.10,95% CI = 1.00-1.20;P heterogeneity = 0.114;I 2 = 53.9%） and population-based studies（G allele vs A allele：OR = 1.12,95% CI = 1.00-1.25;P heterogeneity = 0.062;I 2 = 64.0%）.The findings suggested an association between hsa-mir-499 rs3746444 polymorphism and increased risk to breast cancer.展开更多
人类基因组计划的完成(2001年)宣告了后基因组时代的到来,也掀起新一轮的RNA研究热潮.作为后基因组时代的科学前沿,RNA组学近年来成为生命科学领域的研究热点,各种新型ncRNA的发现,让人们对遗传信息表达调控网络有了新的认识.结合RNA领...人类基因组计划的完成(2001年)宣告了后基因组时代的到来,也掀起新一轮的RNA研究热潮.作为后基因组时代的科学前沿,RNA组学近年来成为生命科学领域的研究热点,各种新型ncRNA的发现,让人们对遗传信息表达调控网络有了新的认识.结合RNA领域的最新研究进展,《中国科学C辑:生命科学》(Science in China Series C-Life Sciences)2009年第3期的8篇述评,从动植物小分子非编码RNA、miRNA与细胞分化发育、miRNA与肿瘤发生及诊断治疗的靶点、核酶的结构与功能、遗传印记起源、miRNA基因簇的进化等多个方面进行了综述,展现了ncRNA领域的研究现状和发展前景.展开更多
文摘Endometrial cancer （EC） is the most common and lethal gynaecological cancer type in Europe and in North America. Frequently EC arises more in the corpus proper and manifests as round, polypoid expansile masses, but it may also originate in the lower uterine segment or spread in endometrium with necrosis and hemorrhage. The analysis was performed using a custom panel containing all DNA sequences loci coding pre-miRNAs and genes related to biogenesis and regulation of sncRNAs in normal and tumor tissues extracted from 6 unrelated patients with endometrial carcinoma. The identified variations were correlated with mature miRNAs differentially expressed in the same normal and tumor endometrial tissues. The comparison analysis confirmed the high degree of cellular and genetic intratumoral heterogeneity with a temporal and spatial miRNA expression distribution in association with genomic variants identified. The classification of specific DNA mutations, onto the loci identified, should be suitable to characterize possible instability genome regions and help classification of tumors to ameliorate the clinical management of patients affected by endometrial carcinoma.
基金supported by China Natural Science Foundation (No.30901534)Jiangsu Province Natural Science Foundation(No. BK2009444)Grant for the 135 Key Medical Project of Jiangsu Province(No.XK201117)
文摘MicroRNAs（miRNAs） are gene regulators involved in numerous diseases including cancer,heart disease,neurological disorders,vascular abnormalities and autoimmune conditions.Although hsa-mir-499 rs3746444 polymorphism was shown to contribute to the susceptibility of multiple genes to cancer,the data have yielded conflicting results.Therefore,this meta-analysis was performed to provide a comprehensive assessment of potential association between hsa-mir-499 rs3746444 polymorphism and cancer risk.In this meta-analysis,a total of 9 articles regarding 10 eligible case-control studies in English（including 6134 cases and 7141 controls） were analyzed.No significant association between hsa-mir-499 rs3746444 polymorphism and overall cancer risk was demonstrated.However,an increased risk was observed in the subgroup of breast cancer patients（G allele vs A allele：OR = 1.10,95% CI = 1.00-1.20;P heterogeneity = 0.114;I 2 = 53.9%） and population-based studies（G allele vs A allele：OR = 1.12,95% CI = 1.00-1.25;P heterogeneity = 0.062;I 2 = 64.0%）.The findings suggested an association between hsa-mir-499 rs3746444 polymorphism and increased risk to breast cancer.
文摘人类基因组计划的完成(2001年)宣告了后基因组时代的到来,也掀起新一轮的RNA研究热潮.作为后基因组时代的科学前沿,RNA组学近年来成为生命科学领域的研究热点,各种新型ncRNA的发现,让人们对遗传信息表达调控网络有了新的认识.结合RNA领域的最新研究进展,《中国科学C辑:生命科学》(Science in China Series C-Life Sciences)2009年第3期的8篇述评,从动植物小分子非编码RNA、miRNA与细胞分化发育、miRNA与肿瘤发生及诊断治疗的靶点、核酶的结构与功能、遗传印记起源、miRNA基因簇的进化等多个方面进行了综述,展现了ncRNA领域的研究现状和发展前景.
