Objective To observe the morphological changes in in vitro growth of preantral follicle isolated from prepuberal mice and to assess impacts of gonadotropin (Gn), insulin transferrin selenium (ITS) and epidermal gr...Objective To observe the morphological changes in in vitro growth of preantral follicle isolated from prepuberal mice and to assess impacts of gonadotropin (Gn), insulin transferrin selenium (ITS) and epidermal growth factor (EGF) on their development. Methods Early preantral mice follicles (90-130μm diameter) were mechanical isolated and selected from 2 weeks' old mice and then cultured in alpha-minimal essential medium (α-MEM) with or without Gn, ITS and EGF. The preantral follicles were cultured singly in 20 miovliters droplets for up to 14 d. The medium was replaced and the .follicles were observed everyday. Granulosa cells (GC) prolification, antrum formation and oocyte maturation were recorded. Results The medium with Gn supported preantral follicle culture in vitro, during which they retained a three-dimensional structure, maintained oocytes viability and increased in diameter and number of somatic cells'. Preantral follicles cultured in Gn medium grew obviously, while those without Gn grew slowly and after 6 d's culture began to shrink and blacken. Significant increase in survival rate and maturation rate of oocytes was observed in Gn group (P〈0.01), with 92.9% survived and 28.7%formed an antrum. Further supplementation of the Gn medium with ITS and rLH, resulted in the significant increase in survival and maturation of preantral follicle (P〈0.05) Conclusions α-MEM can be the medium for in vitro culture (IVC) of preantral follicles, but need to be added with rLH/rFSH, rHCG/rEGF to facilitate thecal cell attachment, GC proliferation and oocyte maturation.展开更多
[Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated un...[Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and serum-free Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.[Result]Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.[Conclusion]There were significant differences(P<0.05)between the growth of cashmere goat hair follicles cultured in the 2 kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium.展开更多
[Objective] The aim of this study was to preliminarily explore the effects of estradiol on morphology and growth of cashmere goat primary hair follicles. [Method] Cashmere goat primary hair follicles were cultured in ...[Objective] The aim of this study was to preliminarily explore the effects of estradiol on morphology and growth of cashmere goat primary hair follicles. [Method] Cashmere goat primary hair follicles were cultured in serum-free Williams E media supplemented with different doses of 17 β-E2 (0, 0.1, 1.0, 10.0, 100.0 nmol/L), and their growth rates and morphological changes were observed. [Result] The growth rate of 0.1 nmol/L 17 β-E2 group was quite comparable with that of the control group(0 nmol/L), but the 17 β-E2 with concentrations of 1.0, 10.0 and 100.0 nmol/L displayed different degrees of inhibition on the growth of hair follicles. Different morphological changes of hair follicles could also be discovered in different concentration treatments. [Conclusion] The study laid a certain foundation for exploring the regulation mechanism of estrogen on growth of cashmere goat hair follicles.展开更多
基金This study was supported by Natural Science Foundation of Shanghai(No.01ZB14037)
文摘Objective To observe the morphological changes in in vitro growth of preantral follicle isolated from prepuberal mice and to assess impacts of gonadotropin (Gn), insulin transferrin selenium (ITS) and epidermal growth factor (EGF) on their development. Methods Early preantral mice follicles (90-130μm diameter) were mechanical isolated and selected from 2 weeks' old mice and then cultured in alpha-minimal essential medium (α-MEM) with or without Gn, ITS and EGF. The preantral follicles were cultured singly in 20 miovliters droplets for up to 14 d. The medium was replaced and the .follicles were observed everyday. Granulosa cells (GC) prolification, antrum formation and oocyte maturation were recorded. Results The medium with Gn supported preantral follicle culture in vitro, during which they retained a three-dimensional structure, maintained oocytes viability and increased in diameter and number of somatic cells'. Preantral follicles cultured in Gn medium grew obviously, while those without Gn grew slowly and after 6 d's culture began to shrink and blacken. Significant increase in survival rate and maturation rate of oocytes was observed in Gn group (P〈0.01), with 92.9% survived and 28.7%formed an antrum. Further supplementation of the Gn medium with ITS and rLH, resulted in the significant increase in survival and maturation of preantral follicle (P〈0.05) Conclusions α-MEM can be the medium for in vitro culture (IVC) of preantral follicles, but need to be added with rLH/rFSH, rHCG/rEGF to facilitate thecal cell attachment, GC proliferation and oocyte maturation.
基金Supported by Regional Key Project of National Natural Science Foundation of China(39969002)Key Project of National Natural Science Foun-dation of Inner Mongolia Autonomous Region(200408020401)~~
文摘[Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and serum-free Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.[Result]Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.[Conclusion]There were significant differences(P<0.05)between the growth of cashmere goat hair follicles cultured in the 2 kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium.
基金Supported by Regional Key Project of National Natural Science Foundation of China(39969002)Key Project of Natural Science Foundation of Inner Mongolia Autonomous Region(200408020401)~~
文摘[Objective] The aim of this study was to preliminarily explore the effects of estradiol on morphology and growth of cashmere goat primary hair follicles. [Method] Cashmere goat primary hair follicles were cultured in serum-free Williams E media supplemented with different doses of 17 β-E2 (0, 0.1, 1.0, 10.0, 100.0 nmol/L), and their growth rates and morphological changes were observed. [Result] The growth rate of 0.1 nmol/L 17 β-E2 group was quite comparable with that of the control group(0 nmol/L), but the 17 β-E2 with concentrations of 1.0, 10.0 and 100.0 nmol/L displayed different degrees of inhibition on the growth of hair follicles. Different morphological changes of hair follicles could also be discovered in different concentration treatments. [Conclusion] The study laid a certain foundation for exploring the regulation mechanism of estrogen on growth of cashmere goat hair follicles.