Diabetes mellitus （DM） is one of the most common ＇metabolic disorders in the world~ in which more than90% are grouped to type 2 DM （T2DM）.1 T2DM is characterized by decreased insulin sensitivity and impaired insu...Diabetes mellitus （DM） is one of the most common ＇metabolic disorders in the world~ in which more than90% are grouped to type 2 DM （T2DM）.1 T2DM is characterized by decreased insulin sensitivity and impaired insulin secretion2 leading to hyperglycemia, and the serum glucose has been used as a golden standard for diabetes diagnosis. However, T2DM is a kind of disease involving defects of multiple organs, which cannot be distinguished through the measurement of the serum-glucose level. In addition, T2DM is a multiple-stage disease, which usually covers several decades from impaired plasma glucose to various complications. The serum-glucose level only reflects the consequence of multiole physiological disorders in the Riven stare.展开更多
Background:Bladder cancer is a common and highly heterogeneous malignant tumor with a relatively poor prognosis.Thus,personalized treatment strategies for bladder cancer are essential for improving patient outcomes.Ma...Background:Bladder cancer is a common and highly heterogeneous malignant tumor with a relatively poor prognosis.Thus,personalized treatment strategies for bladder cancer are essential for improving patient outcomes.Materials and methods:We developed an efficient 3-dimensional in vitro organoid culture system for bladder cancer organoids(BCOs),which maintains the homology with the original patient tumors and the heterogeneity between different individuals.In addition,we constructed chimeric antigen receptor(CAR)-T cells targeting B7H3 and evaluated the antitumor function of CAR-T cells by coculturing them with BCOs.Results:The BCOs closely resembled the characteristics of human tumors and were used to test individual sensitivity to platinum-based drugs and olaparib therapy.Coculture with CAR-T cells demonstrated specific antigen recognition and immune activation,indicating their potential in immunotherapy.Conclusions:Our study highlights the potential of BCOs to facilitate the development of personalized medicine for bladder cancer and improve the efficiency of drug discovery for bladder cancer therapy.展开更多
文摘Diabetes mellitus （DM） is one of the most common ＇metabolic disorders in the world~ in which more than90% are grouped to type 2 DM （T2DM）.1 T2DM is characterized by decreased insulin sensitivity and impaired insulin secretion2 leading to hyperglycemia, and the serum glucose has been used as a golden standard for diabetes diagnosis. However, T2DM is a kind of disease involving defects of multiple organs, which cannot be distinguished through the measurement of the serum-glucose level. In addition, T2DM is a multiple-stage disease, which usually covers several decades from impaired plasma glucose to various complications. The serum-glucose level only reflects the consequence of multiole physiological disorders in the Riven stare.
基金supported by the Key Research and Development Program of Shandong Province(No.2021CXGC01110)the Special Fund for Taishan Scholars Project(No.202211324)+1 种基金the Qingdao Science and Technology Program Project(No,20-3-4-38-nsh)the National Natural Science Foundation of China(No.81970743)..
文摘Background:Bladder cancer is a common and highly heterogeneous malignant tumor with a relatively poor prognosis.Thus,personalized treatment strategies for bladder cancer are essential for improving patient outcomes.Materials and methods:We developed an efficient 3-dimensional in vitro organoid culture system for bladder cancer organoids(BCOs),which maintains the homology with the original patient tumors and the heterogeneity between different individuals.In addition,we constructed chimeric antigen receptor(CAR)-T cells targeting B7H3 and evaluated the antitumor function of CAR-T cells by coculturing them with BCOs.Results:The BCOs closely resembled the characteristics of human tumors and were used to test individual sensitivity to platinum-based drugs and olaparib therapy.Coculture with CAR-T cells demonstrated specific antigen recognition and immune activation,indicating their potential in immunotherapy.Conclusions:Our study highlights the potential of BCOs to facilitate the development of personalized medicine for bladder cancer and improve the efficiency of drug discovery for bladder cancer therapy.
