Suppresssion subtractive hybridization (SSH) was preformed to compare gene expression profiles of PIH patients and normal pregnancy placentas. The subtractive cDNA library of PIH placenta was set up and screedned. Dif...Suppresssion subtractive hybridization (SSH) was preformed to compare gene expression profiles of PIH patients and normal pregnancy placentas. The subtractive cDNA library of PIH placenta was set up and screedned. Differential cDNAs were cloned, and sequenced by T 7 primer methodology. One hundred and three differential cDNAs were isolated by SSH. Sequencing and BLAST analysis showed 90 inserts shared more than 95% homolog with sequences in the GenBank/EMBL database. We identified 36 putative genes including pregnancy-specific glycoprotein gene (BC005924), serine protease inhibitor gene(BC012868), VEGFR-1 gene(AF063657, etc.展开更多
目的探讨肝细胞生长因子(Hepatocyte growth factor,HGF)与胰岛素样生长因子-1(Insu lin like growth fac-tor-1,IGF-1)在正常晚孕妇女及妊娠期高血压疾病患者胎盘组织中的表达以及在妊娠期高血压疾病发病中的作用。方法采用免疫组织学...目的探讨肝细胞生长因子(Hepatocyte growth factor,HGF)与胰岛素样生长因子-1(Insu lin like growth fac-tor-1,IGF-1)在正常晚孕妇女及妊娠期高血压疾病患者胎盘组织中的表达以及在妊娠期高血压疾病发病中的作用。方法采用免疫组织学方法检测62例妊娠期高血压疾病患者(妊娠期高血压疾病组,其中妊娠期高血压21例。轻度子痫前期19例,重度子痫前期22例)及20例正常晚孕妇女(对照组)的胎盘组织中HGF及IGF-1的表达强度,同时HE染色,选取5个高倍视野,计数绒毛血管数。结果(1)HGF主要表达于绒毛间质细胞胞浆,蜕膜细胞有少量表达。重度子痫前期患者胎盘绒毛间质细胞HGF的表达强度显著低于对照组(H=8.548,P=0.003),而妊娠期高血压及轻度子痫前期患者与对照组比较,差异无显著性(H=0.018,P=0.892;H=3.739,P=0.053);(2)IGF-1主要表达于胎盘合体滋养细胞、细胞滋养细胞及蜕膜细胞的胞膜及胞质中。胎盘组织中IGF-1的表达,轻、重度子痫前期患者明显低于对照组(H=4.948,P=0.026;H=15.245,P=0.001)而妊娠期高血压与对照组比较,差异无显著性(H=0.118,P=0.731);(3)不同程度妊娠期高血压疾病组胎盘绒毛血管密度均较对照组减少,重度子痫前期患者胎盘绒毛血管密度为(59.69±3.86)个/×400,与对照组(67.67±4.55)个/×400相比,差异有显著性(P<0.01);(4)相关性分析显示两组孕妇胎盘组织中HGF、IGF-1表达强度分别与绒毛血管密度、新生儿出生体重呈正相关(r=0.695,r=0.386;r=0.270,r=0.782);对照组及妊娠期高血压患者胎盘组织中HGF与IGF-1的表达强度呈强正相关性(r=0.950,P<0.01;r=0.864,P<0.01)。结论胎盘组织中HGF、IGF-1分泌减少与妊娠期高血压疾病发病及病情发展有关,IGF-1可能参与了HGF水平的调节。展开更多
基金Supported in part by the National Natural Science Foundation of China (30070787)
文摘Suppresssion subtractive hybridization (SSH) was preformed to compare gene expression profiles of PIH patients and normal pregnancy placentas. The subtractive cDNA library of PIH placenta was set up and screedned. Differential cDNAs were cloned, and sequenced by T 7 primer methodology. One hundred and three differential cDNAs were isolated by SSH. Sequencing and BLAST analysis showed 90 inserts shared more than 95% homolog with sequences in the GenBank/EMBL database. We identified 36 putative genes including pregnancy-specific glycoprotein gene (BC005924), serine protease inhibitor gene(BC012868), VEGFR-1 gene(AF063657, etc.
文摘目的探讨肝细胞生长因子(Hepatocyte growth factor,HGF)与胰岛素样生长因子-1(Insu lin like growth fac-tor-1,IGF-1)在正常晚孕妇女及妊娠期高血压疾病患者胎盘组织中的表达以及在妊娠期高血压疾病发病中的作用。方法采用免疫组织学方法检测62例妊娠期高血压疾病患者(妊娠期高血压疾病组,其中妊娠期高血压21例。轻度子痫前期19例,重度子痫前期22例)及20例正常晚孕妇女(对照组)的胎盘组织中HGF及IGF-1的表达强度,同时HE染色,选取5个高倍视野,计数绒毛血管数。结果(1)HGF主要表达于绒毛间质细胞胞浆,蜕膜细胞有少量表达。重度子痫前期患者胎盘绒毛间质细胞HGF的表达强度显著低于对照组(H=8.548,P=0.003),而妊娠期高血压及轻度子痫前期患者与对照组比较,差异无显著性(H=0.018,P=0.892;H=3.739,P=0.053);(2)IGF-1主要表达于胎盘合体滋养细胞、细胞滋养细胞及蜕膜细胞的胞膜及胞质中。胎盘组织中IGF-1的表达,轻、重度子痫前期患者明显低于对照组(H=4.948,P=0.026;H=15.245,P=0.001)而妊娠期高血压与对照组比较,差异无显著性(H=0.118,P=0.731);(3)不同程度妊娠期高血压疾病组胎盘绒毛血管密度均较对照组减少,重度子痫前期患者胎盘绒毛血管密度为(59.69±3.86)个/×400,与对照组(67.67±4.55)个/×400相比,差异有显著性(P<0.01);(4)相关性分析显示两组孕妇胎盘组织中HGF、IGF-1表达强度分别与绒毛血管密度、新生儿出生体重呈正相关(r=0.695,r=0.386;r=0.270,r=0.782);对照组及妊娠期高血压患者胎盘组织中HGF与IGF-1的表达强度呈强正相关性(r=0.950,P<0.01;r=0.864,P<0.01)。结论胎盘组织中HGF、IGF-1分泌减少与妊娠期高血压疾病发病及病情发展有关,IGF-1可能参与了HGF水平的调节。
