为了研究不同体况和不同剂量孕马血清促性腺激素(PMSG)对绵羊同期发情效果的影响,试验将体况良好的60只母羊随机分为A、B两组,体况差的120只母羊随机分为C、D两组,A、C组注射400 IU PMSG,B、D组注射330 IU PMSG。结果表明:肌肉注射400 I...为了研究不同体况和不同剂量孕马血清促性腺激素(PMSG)对绵羊同期发情效果的影响,试验将体况良好的60只母羊随机分为A、B两组,体况差的120只母羊随机分为C、D两组,A、C组注射400 IU PMSG,B、D组注射330 IU PMSG。结果表明:肌肉注射400 IU PMSG的母羊发情率优于肌肉注射330 IU PMSG(P<0.05),且体况良好的母羊发情率优于体况差的母羊(P<0.05)。说明母羊体况严重影响同期发情的效果,合理应用生殖激素可以提高绵羊的同期发情率。展开更多
研究不同激素组合处理对小鼠超数排卵及产仔性能的影响,以便获得适宜的超数排卵的激素使用剂量。分别用不同剂量孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)注入小鼠体内进行超数排卵处理,比较激素处理后小鼠卵母细胞成熟率、...研究不同激素组合处理对小鼠超数排卵及产仔性能的影响,以便获得适宜的超数排卵的激素使用剂量。分别用不同剂量孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)注入小鼠体内进行超数排卵处理,比较激素处理后小鼠卵母细胞成熟率、产仔数、胎儿出生重及性别比。结果显示,5 IU PMSG+5 IU HCG剂量组和10 IU PMSG+10 IU HCG剂量组合相比较,卵母细胞成熟率差异不显著(P>0.05),5 IU PMSG+5 IU HCG剂量组平均产仔数最高;10 IU PMSG+10 IU HCG剂量组的胎儿平均初生重最高,但产仔数少。综合以上,在实验中,5 IU PMSG+5 IU HCG剂量组合进行超排处理效果最好。展开更多
Iris du Pré, a professional pianist, wanted a second child, did not conceive quickly and was injected in 1944 by a doctor in Oxford with pregnant mare serum gonadotropin (PMSG). The doctor joked “This child will...Iris du Pré, a professional pianist, wanted a second child, did not conceive quickly and was injected in 1944 by a doctor in Oxford with pregnant mare serum gonadotropin (PMSG). The doctor joked “This child will be a racehorse winner!” In January 1945, Jacqueline du Pré, the remarkable, world-famous cellist was born. In the 1920's and 1930's, animal experimentation and clinical studies had shown that pituitary glycoproteins stimulated the ovary (follicle-stimulating hormone, FSH) and the corpus luteum (luteal-stimulating hormone, LH) which prepared the human womb for embedding a fertilized ovum and that pregnant mare’s blood and urine contained the glycoprotein, PMSG whose origin was placental cells, but surprisingly in humans had the actions of both FSH and LH. However, the PMSG serum alone did not bring about pregnancy. The doctor did not know that without subsequent injection of another factor in correct sequence and timing, PMSG was pointless. In 1947, a placental glycoprotein, found in the 1920's in urine of pregnant women (human chorionic gonadotropin, hCG), when injected in mice subsequent to PMSG, achieved ovulation but not pregnancy. Human application of those findings was extremely risky due to impurities (up to 95%). The Federal Drug Administration (FDA), established in 1938, requested easily bye-passed marketing safety. Companies offered material “sufficiently” purified;professional bodies negated clinical use, tempting to a few. Evidence also suggests that, to sustain pregnancy the doctor also prescribed the new “eostrogen”, diethyl stilbestrol (DES) of negative fame. In 1947, the Nuremberg Code of ethics demanded human experiments by qualified personnel and trials preceded by adequate animal studies. It is not the case here. From five, du Pré had a most exceptional musical memory, almost obsessive musicality and a very difficult school-time socially. Later history: adult masculine build, awkward gait, tendency to recurrent depressions from mid-adolescence, unbalanced thyroidal metabolism, symptoms of numbness in late teens, long breaks for rest from age 25, MS diagnosis at 28 when unable to play, death aged 42. Yet at sixteen and after, she astounded all with technique, passion and unique musical interpretation. Her husband, an outstanding musician: “She had a capacity to imagine sound such as I never met in any other musician”. A close musician colleague: “... it was done before she was born”;perhaps much closer to the truth than realization, for her history it may suggest a fetal neurodevelopment abused in the womb.展开更多
为了建立一种马属动物孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)浓度的检测方法,试验使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)检测商用PMSG抗原纯度,斑点印迹杂交(Dot-blot)判断PMSG和抗体是否结合,蛋白免疫...为了建立一种马属动物孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)浓度的检测方法,试验使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)检测商用PMSG抗原纯度,斑点印迹杂交(Dot-blot)判断PMSG和抗体是否结合,蛋白免疫印迹杂交(Western-blot)确定抗体和PMSG抗原的结合方式,应用酶联免疫吸附试验(ELISA)建立PMSG浓度-吸光度值标准曲线,并拟合方程。结果表明:PMSG抗原纯度符合要求,抗体与PMSG抗原采用空间表位方式结合;PMSG浓度与OD450值的标准曲线拟合方程为y=0.004 5x+0.020 2,R2=0.998,在0~4×102IU/m L范围内,PMSG浓度与OD450值之间高度相关,R2>0.99。说明PMSG抗原可与抗体通过空间表位方式特异性结合,所建立的PMSG免疫学检测方法具有较高的准确性。展开更多
文摘【目的】研究连翘叶提取物(Forsythia suspensa leaves extract,FSLE)对孕马血清促性腺激素(pregnant mare serum gonadotropin,PMSG)在肝脏中代谢的影响,改善在畜牧生产中因PMSG代谢缓慢而造成的氧化应激。【方法】采用半仿生酶醇法提取连翘叶有效成分,给予昆明雌鼠不同含量的FSLE进行动物体内试验;收集小鼠血清,进行酶联免疫吸附试验,检测各组小鼠血清中PMSG的质量浓度;采集小鼠肝脏组织,检测氧化应激指标谷胱甘肽、超氧化物歧化酶、丙二醛和过氧化氢的水平;采用Western-blot检测小鼠肝脏组织孕烷X受体(pregnane X receptor,PXR)和细胞色素酶P45017A1(cytochrome P45017A1,CYP17A1)蛋白的表达水平。【结果】FSLE可显著降低小鼠血清中PMSG的质量浓度,且可以减缓PMSG代谢引起的氧化应激。Western-blot结果显示:FSLE可显著下调小鼠肝组织中PXR和CYP17A1蛋白的表达。【结论】连翘叶提取物可能是通过下调肝脏中PXR和CYP17A1的表达减缓PMSG代谢引起的氧化应激。
文摘为了研究不同体况和不同剂量孕马血清促性腺激素(PMSG)对绵羊同期发情效果的影响,试验将体况良好的60只母羊随机分为A、B两组,体况差的120只母羊随机分为C、D两组,A、C组注射400 IU PMSG,B、D组注射330 IU PMSG。结果表明:肌肉注射400 IU PMSG的母羊发情率优于肌肉注射330 IU PMSG(P<0.05),且体况良好的母羊发情率优于体况差的母羊(P<0.05)。说明母羊体况严重影响同期发情的效果,合理应用生殖激素可以提高绵羊的同期发情率。
文摘研究不同激素组合处理对小鼠超数排卵及产仔性能的影响,以便获得适宜的超数排卵的激素使用剂量。分别用不同剂量孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)注入小鼠体内进行超数排卵处理,比较激素处理后小鼠卵母细胞成熟率、产仔数、胎儿出生重及性别比。结果显示,5 IU PMSG+5 IU HCG剂量组和10 IU PMSG+10 IU HCG剂量组合相比较,卵母细胞成熟率差异不显著(P>0.05),5 IU PMSG+5 IU HCG剂量组平均产仔数最高;10 IU PMSG+10 IU HCG剂量组的胎儿平均初生重最高,但产仔数少。综合以上,在实验中,5 IU PMSG+5 IU HCG剂量组合进行超排处理效果最好。
文摘Iris du Pré, a professional pianist, wanted a second child, did not conceive quickly and was injected in 1944 by a doctor in Oxford with pregnant mare serum gonadotropin (PMSG). The doctor joked “This child will be a racehorse winner!” In January 1945, Jacqueline du Pré, the remarkable, world-famous cellist was born. In the 1920's and 1930's, animal experimentation and clinical studies had shown that pituitary glycoproteins stimulated the ovary (follicle-stimulating hormone, FSH) and the corpus luteum (luteal-stimulating hormone, LH) which prepared the human womb for embedding a fertilized ovum and that pregnant mare’s blood and urine contained the glycoprotein, PMSG whose origin was placental cells, but surprisingly in humans had the actions of both FSH and LH. However, the PMSG serum alone did not bring about pregnancy. The doctor did not know that without subsequent injection of another factor in correct sequence and timing, PMSG was pointless. In 1947, a placental glycoprotein, found in the 1920's in urine of pregnant women (human chorionic gonadotropin, hCG), when injected in mice subsequent to PMSG, achieved ovulation but not pregnancy. Human application of those findings was extremely risky due to impurities (up to 95%). The Federal Drug Administration (FDA), established in 1938, requested easily bye-passed marketing safety. Companies offered material “sufficiently” purified;professional bodies negated clinical use, tempting to a few. Evidence also suggests that, to sustain pregnancy the doctor also prescribed the new “eostrogen”, diethyl stilbestrol (DES) of negative fame. In 1947, the Nuremberg Code of ethics demanded human experiments by qualified personnel and trials preceded by adequate animal studies. It is not the case here. From five, du Pré had a most exceptional musical memory, almost obsessive musicality and a very difficult school-time socially. Later history: adult masculine build, awkward gait, tendency to recurrent depressions from mid-adolescence, unbalanced thyroidal metabolism, symptoms of numbness in late teens, long breaks for rest from age 25, MS diagnosis at 28 when unable to play, death aged 42. Yet at sixteen and after, she astounded all with technique, passion and unique musical interpretation. Her husband, an outstanding musician: “She had a capacity to imagine sound such as I never met in any other musician”. A close musician colleague: “... it was done before she was born”;perhaps much closer to the truth than realization, for her history it may suggest a fetal neurodevelopment abused in the womb.
