Observation on Effect of Gennlanchun (更年春) In Preventing Experimental Primary Osteoporosis

Objective: To evaluate the efficacy of Genni-anchun (GNC) in preventing primary osteoporosis. Methods: Fifty female SD rats of 10 - 12 months old were randomly divided into five groups. Except those in the sham operation group, animals were ovariectomized bilaterally. The gastric perfusion was started immediately after operation, with normal saline to the sham operation group and model group; GNC to high dose (2 ml) and low dos (1 ml) GNC groups and Nilestriol to the NE group, every day for 3 months. Animals were sacrificed at the end of experiment, their vertebra and

Shen(Kidney)-Tonifying Principle for Primary Osteoporosis:to Treat Both the Disease and the Chinese Medicine Syndrome

Primary osteoporosis （POP） is one of the most common diseases in the elderly people resulting in high risk of fracture and poor quality of life. In addition to the pathological changes in bone mass, most of the POP patients also suffer from Chinese medicine （CM） syndromes of Shen （Kidney） essence deficiency. Shen essences are highly related to bone. Shen essence deficiency plays an important part in the development of POP and a better diagnosis of POP could be made by combining CM syndromes with Western medicine risk factors. Treatments of POP should aim at both increasing the bone mass and relieving the syndromes of Shen essence deficiency. Clinical study confirmed that treating POP patients with Shen-tonifying herbs could increase the bone mass and relieve the CM syndromes of POP patients. Basic researches clarified the mechanism by which Shen-tonifying herbs increased bone mass in animal models. The mechanisms by which Shentonifying herbs relieve the CM syndromes are still in investigation.

Clinical Practice Guideline of Traditional Medicine for Primary Osteoporosis

BACKGROUND Primary osteoporosis is a disease characterized by low bone mass and microarchitectural deterioration of bone tissue,leading to enhanced bone fragility and a consequent increase in fracture risk. Primary osteoporosis is particularly common in senile persons and in postmenopausal women.It ranks the fifth of diseases in the modern society.

Identification of Human Serum Protein Targets of Qianggu Decoction(强骨饮) in Primary Type Ⅰ Osteoporosis Based on Tandem Mass Tag Labeling and Liquid Chromatography-Tandem Mass Spectrometry Technology

Objective： To investigate the serum protein targets of Qianggu Decoction（强骨饮, QGD） on treating osteoporosis by the proteomics analysis using tandem mass tag（TMT） and liquid chromatographytandem mass spectrometry（LC-MS/MS）. Methods： Twenty serum protein samples were recruited（10 patients with primary type Ⅰ osteoporosis before and after QGD treatment） and the high abundance ratios protein was removed, two serum samples were extracted and labeled with TMT reagent. Then, mass spectrometric detection, identification of differentially expressed proteins and bioinformatics analysis of differentially expressed proteins were carried out. Results： A total of 60 proteins were identified, within a 99% confidence interval, to be differentially regulated of which, 34 proteins were up-regulated and 26 proteins were down-regulated. Differentially expressed proteins analyzed by Gene Ontology（GO） annotation mainly get involved in 12 different biological processes, 7 types of cellular components, and 6 kinds of molecular functions. Angiotensinogen（AGT）, stromelysin-1（MMP3）, heparanase（HPSE） and glyceraldehyde-3-phosphate dehydrogenase（GAPDH） were screened as candidate protein targets of QGD treatment, which were related to metabolic mechanism of bone remodeling and/or bone collagen of osteoporosis. By the utilization of the protein-protein interaction network analysis tool named STRING10.0, it showed that AGT, MMP3, HPSE and GAPDH were located in the key node of the protein-protein interactions network. Furthermore, AGT, MMP3, HPSE and GAPDH were found to be directly related to BMP, MAPK, Wnt, SMAD and tumor necrosis factor ligand superfamily member 11（TNFSF11） families. Conclusions： The proteomics analysis by using TMT combined with LC-MS/MS was a feasible method for screening the potential therapeutic targets associated with QGD treatment. It suggests that AGT, MMP3, HPSE and GAPDH may be candidate protein targets of QGD treatment which can be used as therapeutic effect monitor and early diagnosis of primary type Ⅰ osteoporosis.