Objective:A protein-based leaking-proof theranostic nanoplatform for dual-modality imaging-guided tumor photodynamic therapy(PDT)has been designed.Impact Statement:A site-specific conjugation of chlorin e6(Ce6)to ferr...Objective:A protein-based leaking-proof theranostic nanoplatform for dual-modality imaging-guided tumor photodynamic therapy(PDT)has been designed.Impact Statement:A site-specific conjugation of chlorin e6(Ce6)to ferrimagnetic ferritin(MFtn-Ce6)has been constructed to address the challenge of unexpected leakage that often occurs during small-molecule drug delivery.Introduction:PDT is one of the most promising approaches for tumor treatment,while a delivery system is typically required for hydrophobic photosensitizers.However,the nonspecific distribution and leakage of photosensitizers could lead to insufficient drug accumulation in tumor sites.Methods:An engineered ferritin was generated for site-specific conjugation of Ce6 to obtain a leaking-proof delivery system,and a ferrimagnetic core was biomineralized in the cavity of ferritin,resulting in a fluorescent ferrimagnetic ferritin nanoplatform(MFtn-Ce6).The distribution and tumor targeting of MFtn-Ce6 can be detected by magnetic resonance imaging(MRI)and fluorescence imaging(FLI).Results:MFtn-Ce6 showed effective dual-modality MRI and FLI.A prolonged in vivo circulation and increased tumor accumulation and retention of photosensitizer was observed.The time-dependent distribution of MFtn-Ce6 can be precisely tracked in real time to find the optimal time window for PDT treatment.The colocalization of ferritin and the iron oxide core confirms the high stability of the nanoplatform in vivo.The results showed that mice treated with MFtn-Ce6 exhibited marked tumor-suppressive activity after laser irradiation.Conclusion:The ferritin-based leaking-proof nanoplatform can be used for the efficient delivery of the photosensitizer to achieve an enhanced therapeutic effect.This method established a general approach for the dual-modality imagingguided tumor delivery of PDT agents.展开更多
Since transactions in blockchain are based on public ledger verification,this raises security concerns about privacy protection.And it will cause the accumulation of data on the chain and resulting in the low efficien...Since transactions in blockchain are based on public ledger verification,this raises security concerns about privacy protection.And it will cause the accumulation of data on the chain and resulting in the low efficiency of block verification,when the whole transaction on the chain is verified.In order to improve the efficiency and privacy protection of block data verification,this paper proposes an efficient block verification mechanism with privacy protection based on zeroknowledge proof(ZKP),which not only protects the privacy of users but also improves the speed of data block verification.There is no need to put the whole transaction on the chain when verifying block data.It just needs to generate the ZKP and root hash with the transaction information,then save them to the smart contract for verification.Moreover,the ZKP verification in smart contract is carried out to realize the privacy protection of the transaction and efficient verification of the block.When the data is validated,the buffer accepts the complete transaction,updates the transaction status in the cloud database,and packages up the chain.So,the ZKP strengthens the privacy protection ability of blockchain,and the smart contracts save the time cost of block verification.展开更多
This scientific paper is a comparative analysis of two mathematical conjectures. The newly proposed -3(-n) - 1 Remer conjecture and how it is related to and a proof of the more well known 3n + 1 Collatz conjecture. An...This scientific paper is a comparative analysis of two mathematical conjectures. The newly proposed -3(-n) - 1 Remer conjecture and how it is related to and a proof of the more well known 3n + 1 Collatz conjecture. An overview of both conjectures and their respective iterative processes will be presented. Showcasing their unique properties and behavior to each other. Through a detailed comparison, we highlight the similarities and differences between these two conjectures and discuss their significance in the field of mathematics. And how they prove each other to be true.展开更多
This article offers a simple but rigorous proof of Brouwer’s fixed point theorem using Sperner’s Lemma.The general method I have used so far in the proof is mainly to convert the n-dimensional shapes to the correspo...This article offers a simple but rigorous proof of Brouwer’s fixed point theorem using Sperner’s Lemma.The general method I have used so far in the proof is mainly to convert the n-dimensional shapes to the corresponding case under the Sperner’s Labeling and apply the Sperner’s Lemma to solve the question.展开更多
基金This work was supported by the National Key R&D Program of China(2020YFA0710700 and 2017YFA0505400)the National Natural Science Foundation of China(52021002,21877103,22177109,and 32101121)+2 种基金Users with Excellence Program of Hefei Science Center CAS(2020HSC-UE016)the Fundamental Research Funds for the Central Universities(WK3450000007)Suzhou Scientific and Technological Project(SYG202017).
文摘Objective:A protein-based leaking-proof theranostic nanoplatform for dual-modality imaging-guided tumor photodynamic therapy(PDT)has been designed.Impact Statement:A site-specific conjugation of chlorin e6(Ce6)to ferrimagnetic ferritin(MFtn-Ce6)has been constructed to address the challenge of unexpected leakage that often occurs during small-molecule drug delivery.Introduction:PDT is one of the most promising approaches for tumor treatment,while a delivery system is typically required for hydrophobic photosensitizers.However,the nonspecific distribution and leakage of photosensitizers could lead to insufficient drug accumulation in tumor sites.Methods:An engineered ferritin was generated for site-specific conjugation of Ce6 to obtain a leaking-proof delivery system,and a ferrimagnetic core was biomineralized in the cavity of ferritin,resulting in a fluorescent ferrimagnetic ferritin nanoplatform(MFtn-Ce6).The distribution and tumor targeting of MFtn-Ce6 can be detected by magnetic resonance imaging(MRI)and fluorescence imaging(FLI).Results:MFtn-Ce6 showed effective dual-modality MRI and FLI.A prolonged in vivo circulation and increased tumor accumulation and retention of photosensitizer was observed.The time-dependent distribution of MFtn-Ce6 can be precisely tracked in real time to find the optimal time window for PDT treatment.The colocalization of ferritin and the iron oxide core confirms the high stability of the nanoplatform in vivo.The results showed that mice treated with MFtn-Ce6 exhibited marked tumor-suppressive activity after laser irradiation.Conclusion:The ferritin-based leaking-proof nanoplatform can be used for the efficient delivery of the photosensitizer to achieve an enhanced therapeutic effect.This method established a general approach for the dual-modality imagingguided tumor delivery of PDT agents.
基金This work was supported by China’s National Natural Science Foundation(No.62072249,62072056).Jin Wang and Yongjun Ren received the grant and the URLs to sponsors’websites are https://www.nsfc.gov.cn/.This work was also funded by the Researchers Supporting Project No.(RSP-2021/102)King Saud University,Riyadh,Saudi Arabia.
文摘Since transactions in blockchain are based on public ledger verification,this raises security concerns about privacy protection.And it will cause the accumulation of data on the chain and resulting in the low efficiency of block verification,when the whole transaction on the chain is verified.In order to improve the efficiency and privacy protection of block data verification,this paper proposes an efficient block verification mechanism with privacy protection based on zeroknowledge proof(ZKP),which not only protects the privacy of users but also improves the speed of data block verification.There is no need to put the whole transaction on the chain when verifying block data.It just needs to generate the ZKP and root hash with the transaction information,then save them to the smart contract for verification.Moreover,the ZKP verification in smart contract is carried out to realize the privacy protection of the transaction and efficient verification of the block.When the data is validated,the buffer accepts the complete transaction,updates the transaction status in the cloud database,and packages up the chain.So,the ZKP strengthens the privacy protection ability of blockchain,and the smart contracts save the time cost of block verification.
文摘This scientific paper is a comparative analysis of two mathematical conjectures. The newly proposed -3(-n) - 1 Remer conjecture and how it is related to and a proof of the more well known 3n + 1 Collatz conjecture. An overview of both conjectures and their respective iterative processes will be presented. Showcasing their unique properties and behavior to each other. Through a detailed comparison, we highlight the similarities and differences between these two conjectures and discuss their significance in the field of mathematics. And how they prove each other to be true.
基金by Dr Kemp from National Mathematics and Science College.
文摘This article offers a simple but rigorous proof of Brouwer’s fixed point theorem using Sperner’s Lemma.The general method I have used so far in the proof is mainly to convert the n-dimensional shapes to the corresponding case under the Sperner’s Labeling and apply the Sperner’s Lemma to solve the question.
