The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system is a fast-growing,genome editing technology that has wide applications in identifying gene functions as wel...The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system is a fast-growing,genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding.Here,we summarized recent advances in the development and applications of genome editing technologies in plants.We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology.Some new prime editing-derived techniques were assessed.展开更多
基金National Natural Science Foundation of China(NSFC)(31571464,31371438,and 31070222 to Q.S.Q.)Qinghai Provincial Department of Science and Technology Qinghai basic research program(2022-ZJ-724 to Q.S.Q.)+3 种基金Qinghai Provincial Department of Science and Technology Innovation Platform Construction Fund(2020-ZJ-Y40 to Q.S.Q.)Independent Research and Development Project of State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems(202202 to Q.S.Q.)Special Project in Key Fields of the Ordinary Universities of Guangdong Provincial Department(2021ZDZX4027)Innovation Team Project of Ordinary Universities of Guangdong Province(2021KCXTD011).
文摘The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system is a fast-growing,genome editing technology that has wide applications in identifying gene functions as well as improving agricultural production and crop breeding.Here,we summarized recent advances in the development and applications of genome editing technologies in plants.We briefly described CRISPR/Cas9 technology and examined the base and prime editing techniques that have been developed from CRISPR technology.Some new prime editing-derived techniques were assessed.
