Developmental potency of primitive and embryonic ectoderm cells from 4.50-day to 6.25-day post-coitum (p.c.) mouse embryos and primordial germ cells from 12.50-day p.c.male genital ridges of fetal mice were studied by...Developmental potency of primitive and embryonic ectoderm cells from 4.50-day to 6.25-day post-coitum (p.c.) mouse embryos and primordial germ cells from 12.50-day p.c.male genital ridges of fetal mice were studied by direct introducing them into 3.50-day p.c.blastocysts.Sixteen (61.5) overt chimaeras out of 26(50%) offsprings were obtained after transfer of 52 blastocysts injected with 4.50-day primitive ectoderm cells;four (16.0%) overt chimaeras were obtained out of 25 (51.0%) offsprings with 4.75-day primitive ectoderm cells from 49 transferred blastocysts.However,no overt chimaera was obtained with either 5.25-day or 6.25-day embryonic ectoderm cells or 12.50-day male primordial germ cells.GPI analysis of mid-gestation conceptuses developed from injected blastocysts showedthat 5.25-day embryonic ectoderm cells could only contributed to yolk sac of conceptus.Results suggested that implantation acts as a trigger for the determination of primitive ectoderm cells,and their developmental potency becomes limited within a short period of time in normal development.展开更多
Inbred of Wu Zhishan miniature pig(WZSP)at 5 to 8 Mouths of age,which were used as embryo donors.The total 75 fetuses was collected from 14 donors at day 25 to 29 of pregnancy(estrus=day 0),which was used for in vitro...Inbred of Wu Zhishan miniature pig(WZSP)at 5 to 8 Mouths of age,which were used as embryo donors.The total 75 fetuses was collected from 14 donors at day 25 to 29 of pregnancy(estrus=day 0),which was used for in vitro culture,clone,isolation,passage of porcine embryonic germ(PEG).The results were shown that:(1)The PGCs of fetuses between days 2729 of pregnancy(estrus=0)was easily to collect and more suitable to set up PEGs.(2)The PEGs clones with WZSP was coming out 2 days early than Shim(97),Piedrahita(98)and Mueller(99)reported previously.(3)PEG′s will be survival in vitro after thawing.展开更多
Coilia nasus is an endangered fish species in the Yangtze River,and there is urgent need to know the genes related to its reproduction and fertility.The DAZ family including boule,daz and dazl,plays an important role ...Coilia nasus is an endangered fish species in the Yangtze River,and there is urgent need to know the genes related to its reproduction and fertility.The DAZ family including boule,daz and dazl,plays an important role in germ cells development.In this study,the cDNA sequence of boule(Cnbol)and dazl(Cndazl)genes were cloned and their expression patterns were characterized in Coilia nasus.RT-PCR showed that the expression of Cnbol and Cndazl mRNAs was restricted in adult gonads.The section in situ hybridization indicated that the temporospatial expression patterns of Cnbol and Cndazl were significantly different.In the testis,Cnbol was mainly detected in spermatocytes and spermatids,while Cndazl was prominently expressed in s permatogonia.In the ovary,Cnbol and Cndazl were highly expressed in the early stages of oocytes.Interestingly,Cndazl was also concentrated in perinuclear speckle and then localized to the Balbiani body in late stages of oocytes.In addition,Cnbol and Cndazl 3′UTR can specifically label medaka Primordial Germ Cells(PGCs).According to our results,Cnbol and Cndazl are marker genes of germ cells and may play a vital role in the development and differentiation of germ cells in Coilia nasus.展开更多
文摘Developmental potency of primitive and embryonic ectoderm cells from 4.50-day to 6.25-day post-coitum (p.c.) mouse embryos and primordial germ cells from 12.50-day p.c.male genital ridges of fetal mice were studied by direct introducing them into 3.50-day p.c.blastocysts.Sixteen (61.5) overt chimaeras out of 26(50%) offsprings were obtained after transfer of 52 blastocysts injected with 4.50-day primitive ectoderm cells;four (16.0%) overt chimaeras were obtained out of 25 (51.0%) offsprings with 4.75-day primitive ectoderm cells from 49 transferred blastocysts.However,no overt chimaera was obtained with either 5.25-day or 6.25-day embryonic ectoderm cells or 12.50-day male primordial germ cells.GPI analysis of mid-gestation conceptuses developed from injected blastocysts showedthat 5.25-day embryonic ectoderm cells could only contributed to yolk sac of conceptus.Results suggested that implantation acts as a trigger for the determination of primitive ectoderm cells,and their developmental potency becomes limited within a short period of time in normal development.
文摘Inbred of Wu Zhishan miniature pig(WZSP)at 5 to 8 Mouths of age,which were used as embryo donors.The total 75 fetuses was collected from 14 donors at day 25 to 29 of pregnancy(estrus=day 0),which was used for in vitro culture,clone,isolation,passage of porcine embryonic germ(PEG).The results were shown that:(1)The PGCs of fetuses between days 2729 of pregnancy(estrus=0)was easily to collect and more suitable to set up PEGs.(2)The PEGs clones with WZSP was coming out 2 days early than Shim(97),Piedrahita(98)and Mueller(99)reported previously.(3)PEG′s will be survival in vitro after thawing.
基金supported by the National Key R&D Program of China(2018YFD0901205)National Natural Science Foundation of China(31672700).
文摘Coilia nasus is an endangered fish species in the Yangtze River,and there is urgent need to know the genes related to its reproduction and fertility.The DAZ family including boule,daz and dazl,plays an important role in germ cells development.In this study,the cDNA sequence of boule(Cnbol)and dazl(Cndazl)genes were cloned and their expression patterns were characterized in Coilia nasus.RT-PCR showed that the expression of Cnbol and Cndazl mRNAs was restricted in adult gonads.The section in situ hybridization indicated that the temporospatial expression patterns of Cnbol and Cndazl were significantly different.In the testis,Cnbol was mainly detected in spermatocytes and spermatids,while Cndazl was prominently expressed in s permatogonia.In the ovary,Cnbol and Cndazl were highly expressed in the early stages of oocytes.Interestingly,Cndazl was also concentrated in perinuclear speckle and then localized to the Balbiani body in late stages of oocytes.In addition,Cnbol and Cndazl 3′UTR can specifically label medaka Primordial Germ Cells(PGCs).According to our results,Cnbol and Cndazl are marker genes of germ cells and may play a vital role in the development and differentiation of germ cells in Coilia nasus.
