Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells

Primula vulgaris belongs to the genus Primula, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Primula species, but there have been limited studies on the cytotoxic effect of P vulgaris. The aim of this study was to investigate the cytotoxic effects, and possible mechanisms involved, of P vulgaris flower extract on human cervical cancer （HeLa） cells. The cytotoxic effect of the extract on HeLa cells was revealed using the MTT assay. Mechanisms involved in the extract＇s cytotoxic effect were then investigated in terms of apoptosis, mitochondrial membrane potential, and the cell cycle, using fluorometric methods. P vulgaris flower extract exhibited selective cytotoxic effects against HeLa cells by arresting their cell cycle at the S phase, and inducing the number of apoptotic cells compared to normal fibroblast cells by reducing mitochondrial membrane potential in a concentration-dependent manner. This is the first study to reveal the antiproliferative effect ofP. vulgaris flower extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms.

基于SSR标记的38份欧洲报春种质资源遗传多样性分析

为加快欧洲报春育种进程,本研究以‘庆典’、‘丹诺雅’、‘丹诺雅XL’、‘丹妮莎’、‘普利罗曼’以及上海源怡种苗股份有限公司自主育种的‘春晖’系列共38个欧报春品种为材料,利用24对SSR引物通过毛细管电泳检测法进行了欧洲报春遗传多样性和遗传关系分析。供试的24对SSR引物中11对引物稳定性和多态性较好,可用于遗传多样性和聚类分析。多态性信息指数为0.360 3~0.953 3,平均值为0.814 9。38个欧洲报春品种分别来源于6个不同品系,6个品系遗传距离都维持在较高水平,其中‘丹妮莎’和‘普利罗曼’间的遗传距离最小,上海源怡种苗股份有限公司自主育种的‘春晖’系列与‘庆典’的遗传距离最大,且‘春晖’系列与其余5个品系的遗传距离均大于0.5。通过STRUCTURE分析发现6个群体中存在5个基因库,且不同品系、不同花色的品种在基因库组成比例存在较大差异。本研究初步通过已有SSR标记开展欧洲报春遗传多样性分析,筛选出的标记可用于不同品系遗传距离的分析,但在不同品种DNA指纹图谱构建中还存在一定欠缺。后续将通过多种途径开展欧洲报春SSR标记的开发,以推进欧洲报春的育种进程以及新品种鉴定工作。