本研究通过高糖高脂饮食(high-fat and high-sugar diet,HSHF)建立SD大鼠代谢综合征模型,旨在探究葡萄籽原花青素对代谢综合征大鼠的体质量、血糖浓度、胰岛素敏感性、血清脂质水平和炎症的干预作用。结果表明,与正常对照组相比,24周后...本研究通过高糖高脂饮食(high-fat and high-sugar diet,HSHF)建立SD大鼠代谢综合征模型,旨在探究葡萄籽原花青素对代谢综合征大鼠的体质量、血糖浓度、胰岛素敏感性、血清脂质水平和炎症的干预作用。结果表明,与正常对照组相比,24周后,喂食HSHF的大鼠出现腹部肥胖、葡萄糖耐量降低、动脉粥样硬化性血脂紊乱、肝脏脂肪沉积。葡萄籽原花青素(20、40、80 mg/kg mb)干预大鼠8周后,空腹血糖、血清甘油三酯、总胆固醇、低密度脂蛋白胆固醇、炎症因子白细胞介素-6和肿瘤坏死因子-α水平均显著降低,高密度脂蛋白胆固醇水平显著升高。苏木精-伊红切片分析结果表明,葡萄籽原花青素能够减小脂肪组织细胞脂滴面积,减轻了肝脏脂肪沉积。葡萄籽原花青素降低代谢综合征大鼠的体质量,改善大鼠血糖和脂代谢紊乱,以及降低血清炎症因子水平,因此有望作为潜在的调节代谢综合征的天然活性产物。展开更多
The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant,they are also important in their ornamental appeal in Narcissus plant.I...The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant,they are also important in their ornamental appeal in Narcissus plant.In this study,we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor.Comparative transcriptome analysis of lossand gain-of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways.Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS,NtLAR,and NtDFR that were cloned by genome walking assay,and activated NtFLS and NtLAR expression but repressed NtDFR expression.More interestingly,NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptomebased WGCNA and confirmed by yeast two hybrid,bimolecular fluorescence complementation and coimmunoprecipitation assay.Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted(pro)anthocyanin accumulation,while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols,but repressed NtDFR expression.These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and(pro)anthocyanin biosynthesis.展开更多
基金Open access funding provided by Shanghai Jiao Tong Universitysupported by the“100 talent scientist plan”program of Fujian Province of China to Ying Miao.
文摘The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant,they are also important in their ornamental appeal in Narcissus plant.In this study,we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor.Comparative transcriptome analysis of lossand gain-of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways.Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS,NtLAR,and NtDFR that were cloned by genome walking assay,and activated NtFLS and NtLAR expression but repressed NtDFR expression.More interestingly,NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptomebased WGCNA and confirmed by yeast two hybrid,bimolecular fluorescence complementation and coimmunoprecipitation assay.Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted(pro)anthocyanin accumulation,while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols,but repressed NtDFR expression.These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and(pro)anthocyanin biosynthesis.
