Our previous study has revealed that procyanidin A_(1)(A_(1))and its simulated digestive product(D-A,)can alleviate acrylamide(ACR)-induced intestine cell damage.However,the underlying mechanism remains unknown.In thi...Our previous study has revealed that procyanidin A_(1)(A_(1))and its simulated digestive product(D-A,)can alleviate acrylamide(ACR)-induced intestine cell damage.However,the underlying mechanism remains unknown.In this study,we elucidated the molecular mechanism for and D-A_(1) to alleviate ACR-stimulated IPEC-J2 cell damage.ACR slightly activated nuclear factor erythroid 2-related factor 2(Nrf2)signaling and its target genes,but this activation could not reduce intestine cell damage.A_(1) and D-A_(1) could alleviate ACR-induced cell damage,but the effect was abrogated in cells transiently transfected with Nrf2 small interfering RNA(siRNA).Further investigation confirmed that A_(1) and D-A_(1) interacted with Ketch-like ECH-associated protein 1(Keapl),which boosted the stabilization of Nrf2,subsequently promoted the translocation of Nrf2 into the nucleus,and further increased the expression of antioxidant proteins,thereby inhibiting glutathione(GSH)consumption,maintaining redox balance and eventually alleviating ACR-induced cell damage.Importantly,there was no difference between A_(1) and D-A_(1) treated groups,indicating that A_(1) can tolerate gastrointestinal digestion and may be a potential compound to limit the toxicity of ACR.展开更多
The 6061 aluminum matrix composites reinforced with ZnO-coated Mg_2B_2O_5w were fabricated by squeeze casting method and followed by extruded under a technical equivalent condition. The mechanical properties and micro...The 6061 aluminum matrix composites reinforced with ZnO-coated Mg_2B_2O_5w were fabricated by squeeze casting method and followed by extruded under a technical equivalent condition. The mechanical properties and microstructures of the composites were investigated. The results showed that the elastic modulus of the as-cast composites increased straightly with increasing ZnO coating content. The ultimate tensile strength and yield strength of the as-cast composites rapidly increased initially and then declined with increasing ZnO coating content. However, the elongations of all the as-cast composites had similar values. The elongations of the composites were highly enhanced and the ultimate tensile strength of the composite without ZnO coating was the largest after extrusion. A number of whiskers in the composites with ZnO coating were fractured during the extrusion process, but the whiskers＇ breakage extent was limited with the increase of coating content.展开更多
目的:探讨2型糖尿病患者血清血栓素B_2(TXB_2)水平变化及临床意义。方法:选择本院2014年7-12月收治的78例2型糖尿病患者作为病例组,并选择同时期本院门诊健康体检者80例作为对照组,采用放射免疫分析法测定两组对象血清TXB_2和内皮素(ET...目的:探讨2型糖尿病患者血清血栓素B_2(TXB_2)水平变化及临床意义。方法:选择本院2014年7-12月收治的78例2型糖尿病患者作为病例组,并选择同时期本院门诊健康体检者80例作为对照组,采用放射免疫分析法测定两组对象血清TXB_2和内皮素(ET)水平,通过Pearson相关分析法分析病例组TXB_2、ET与一般资料的相关性。结果:病例组血清TXB_2、ET水平均显著高于对照组,且病例组血清TXB_2、ET水平均随病程、Hb A1c、PBG、2 h PBG增加而升高,差异均有统计学意义(P<0.05);患者TXB_2、ET与病程、Hb A1c、PBG、2 h PBG均存在显著正相关性(P<0.05)。结论:2型糖尿病患者血清TXB_2、ET水平明显升高,应及时采取有效治疗措施将其血糖水平控制在正常范围内。展开更多
Objective This study aimed to explore the protective effect of procyanidin B2(PCB2)on acute liver injury induced by aflatoxin B1(AFB1)in rats.Methods Forty Sprague Dawley rats were randomly divided into control,AFB1,A...Objective This study aimed to explore the protective effect of procyanidin B2(PCB2)on acute liver injury induced by aflatoxin B1(AFB1)in rats.Methods Forty Sprague Dawley rats were randomly divided into control,AFB1,AFB1+PCB2,and PCB2 groups.The latter two groups were administrated PCB2 intragastrically(30 mg/kg body weight)for 7 d,whereas the control and AFB1 groups were given the same dose of double distilled water intragastrically.On the sixth day of treatment,the AFB1 and AFB1+PCB2 groups were intraperitoneally injected with AFB1(2 mg/kg).The control and PCB2 groups were intraperitoneally administered the same dose of dimethyl sulfoxide(DMSO).On the eighth day,all rats were euthanized:serum and liver tissue were isolated for further examination.Hepatic histological features were assessed by hematoxylin and eosin-stained sections.Weight,organ coefficient(liver,spleen,and kidney),liver function(serum alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,total bilirubin,and direct bilirubin),oxidative index(catalase,glutathione,superoxide dismutase,malondialdehyde,and 8-hydroxy-2′-deoxyguanosine),inflammation factor[hepatic interleukin-6(IL-6)m RNA expression and serum IL-6],and bcl-2/bax ratio were measured.Results AFB1 significantly caused hepatic histopathological damage,abnormal liver function,oxidative stress,inflammation,and bcl-2/bax ratio reduction compared with DMSO-treated controls.Our results indicate that PCB2 treatment can partially reverse the adverse liver conditions induced by AFB1.Conclusion Our findings indicate that PCB2 exhibits a protective effect on acute liver injury induced by AFB1.展开更多
基金supported by the project from National Natural Science Foundation of China (31671962)Fundamental Research Funds for the Central Universities (2662019PY034)。
文摘Our previous study has revealed that procyanidin A_(1)(A_(1))and its simulated digestive product(D-A,)can alleviate acrylamide(ACR)-induced intestine cell damage.However,the underlying mechanism remains unknown.In this study,we elucidated the molecular mechanism for and D-A_(1) to alleviate ACR-stimulated IPEC-J2 cell damage.ACR slightly activated nuclear factor erythroid 2-related factor 2(Nrf2)signaling and its target genes,but this activation could not reduce intestine cell damage.A_(1) and D-A_(1) could alleviate ACR-induced cell damage,but the effect was abrogated in cells transiently transfected with Nrf2 small interfering RNA(siRNA).Further investigation confirmed that A_(1) and D-A_(1) interacted with Ketch-like ECH-associated protein 1(Keapl),which boosted the stabilization of Nrf2,subsequently promoted the translocation of Nrf2 into the nucleus,and further increased the expression of antioxidant proteins,thereby inhibiting glutathione(GSH)consumption,maintaining redox balance and eventually alleviating ACR-induced cell damage.Importantly,there was no difference between A_(1) and D-A_(1) treated groups,indicating that A_(1) can tolerate gastrointestinal digestion and may be a potential compound to limit the toxicity of ACR.
基金Project(2011CB612200)supported by the National Basic Research Program of China
文摘The 6061 aluminum matrix composites reinforced with ZnO-coated Mg_2B_2O_5w were fabricated by squeeze casting method and followed by extruded under a technical equivalent condition. The mechanical properties and microstructures of the composites were investigated. The results showed that the elastic modulus of the as-cast composites increased straightly with increasing ZnO coating content. The ultimate tensile strength and yield strength of the as-cast composites rapidly increased initially and then declined with increasing ZnO coating content. However, the elongations of all the as-cast composites had similar values. The elongations of the composites were highly enhanced and the ultimate tensile strength of the composite without ZnO coating was the largest after extrusion. A number of whiskers in the composites with ZnO coating were fractured during the extrusion process, but the whiskers＇ breakage extent was limited with the increase of coating content.
文摘目的:探讨2型糖尿病患者血清血栓素B_2(TXB_2)水平变化及临床意义。方法:选择本院2014年7-12月收治的78例2型糖尿病患者作为病例组,并选择同时期本院门诊健康体检者80例作为对照组,采用放射免疫分析法测定两组对象血清TXB_2和内皮素(ET)水平,通过Pearson相关分析法分析病例组TXB_2、ET与一般资料的相关性。结果:病例组血清TXB_2、ET水平均显著高于对照组,且病例组血清TXB_2、ET水平均随病程、Hb A1c、PBG、2 h PBG增加而升高,差异均有统计学意义(P<0.05);患者TXB_2、ET与病程、Hb A1c、PBG、2 h PBG均存在显著正相关性(P<0.05)。结论:2型糖尿病患者血清TXB_2、ET水平明显升高,应及时采取有效治疗措施将其血糖水平控制在正常范围内。
基金financially supported by National Natural Science Foundation of China[No.31360383]。
文摘Objective This study aimed to explore the protective effect of procyanidin B2(PCB2)on acute liver injury induced by aflatoxin B1(AFB1)in rats.Methods Forty Sprague Dawley rats were randomly divided into control,AFB1,AFB1+PCB2,and PCB2 groups.The latter two groups were administrated PCB2 intragastrically(30 mg/kg body weight)for 7 d,whereas the control and AFB1 groups were given the same dose of double distilled water intragastrically.On the sixth day of treatment,the AFB1 and AFB1+PCB2 groups were intraperitoneally injected with AFB1(2 mg/kg).The control and PCB2 groups were intraperitoneally administered the same dose of dimethyl sulfoxide(DMSO).On the eighth day,all rats were euthanized:serum and liver tissue were isolated for further examination.Hepatic histological features were assessed by hematoxylin and eosin-stained sections.Weight,organ coefficient(liver,spleen,and kidney),liver function(serum alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,total bilirubin,and direct bilirubin),oxidative index(catalase,glutathione,superoxide dismutase,malondialdehyde,and 8-hydroxy-2′-deoxyguanosine),inflammation factor[hepatic interleukin-6(IL-6)m RNA expression and serum IL-6],and bcl-2/bax ratio were measured.Results AFB1 significantly caused hepatic histopathological damage,abnormal liver function,oxidative stress,inflammation,and bcl-2/bax ratio reduction compared with DMSO-treated controls.Our results indicate that PCB2 treatment can partially reverse the adverse liver conditions induced by AFB1.Conclusion Our findings indicate that PCB2 exhibits a protective effect on acute liver injury induced by AFB1.
