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基质辅助激光解吸电离飞行时间质谱在流感嗜血杆菌鉴定中的应用
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作者 张雪英 叶慧 《实用检验医师杂志》 2024年第3期270-273,共4页
目的分析基质辅助激光解吸电离飞行时间质谱(MALDI-TOP MS)在流感嗜血杆菌鉴定中的应用。方法收集2022年1—12月南京艾迪康医学检验所有限公司实验室接收的1300份呼吸道感染患者的痰液或咽拭子样本培养和分离的嗜血杆菌,经16SrDNA序列... 目的分析基质辅助激光解吸电离飞行时间质谱(MALDI-TOP MS)在流感嗜血杆菌鉴定中的应用。方法收集2022年1—12月南京艾迪康医学检验所有限公司实验室接收的1300份呼吸道感染患者的痰液或咽拭子样本培养和分离的嗜血杆菌,经16SrDNA序列测定检出50株为流感嗜血杆菌;分别使用MALDI-TOP MS检测系统、Analytic Products INC(API)生化细菌鉴定系统以及纸片法卫星试验对流感嗜血杆菌进行鉴定,比较并分析3种方法对流感嗜血杆菌的检出率、检测时间、检测成本及读取方式。结果MALDI-TOP MS检测系统对流感嗜血杆菌的检出率为98.0%,API生化细菌鉴定系统的检出率为90.0%,纸片法卫星试验的检出率为74.0%。MALDI-TOP MS对流感嗜血杆菌的检出率明显高于纸片法卫星试验,差异有统计学意义(P<0.05),而与API生化细菌鉴定系统比较检出率差异无统计学意义(P>0.05)。MALDI-TOP MS检测所用时间为5~10 min,API生化细菌鉴定检测所用时间为4~24 h,纸片法卫星试验检测所用时间为24~48 h。采用MALDI-TOP MS鉴定流感嗜血杆菌的成本更低,传统API生化细菌鉴定方法价格昂贵,而纸片法卫星试验操作比较繁琐。结论MALDI-TOP MS检测系统对流感嗜血杆菌鉴定的准确率较高,鉴定周期较短,成本较低,且使用方便,在临床上对流感嗜血杆菌的检出具有重要价值。 展开更多
关键词 基质辅助激光解吸电离飞行时间质谱 流感嗜血杆菌 Analytic products INC生化细菌鉴定系统 纸片法卫星试验
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Some Properties of Two Class of Analytic Functions Family 被引量:2
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作者 凌怡 《Chinese Quarterly Journal of Mathematics》 CSCD 1992年第1期96-100,共5页
Let A be the class of functions f(z)=z+sum from n=2 to ∞ (a_nZ^n) which are analytic in the unit disc, and let In this paper, Some properties of Q_α(β) and R_α(β) are investigated. In particular, Some results due... Let A be the class of functions f(z)=z+sum from n=2 to ∞ (a_nZ^n) which are analytic in the unit disc, and let In this paper, Some properties of Q_α(β) and R_α(β) are investigated. In particular, Some results due to chichra [4], Mocanu[5] and Obradovic[6] are extended. In addition, We also showed an error of S. Owa[8]. 展开更多
关键词 analytic function Hadamard product univalent function deviation theorem
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Prediction of rock brittleness using nondestructive methods for hard rock tunneling 被引量:6
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作者 Rennie B.Kaunda Brian Asbury 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2016年第4期533-540,共8页
Sand production is an undesired phenomenon occurring in unconsolidated formations due to shear failure and hydrodynamic forces. There have been many approaches developed to predict sand production and prevent it by ch... Sand production is an undesired phenomenon occurring in unconsolidated formations due to shear failure and hydrodynamic forces. There have been many approaches developed to predict sand production and prevent it by changing drilling or production strategies. However, assumptions involved in these approaches have limited their applications to very specific scenarios. In this paper, an elliptical model based on the borehole shape is presented to predict the volume of sand produced during the drilling and depletion stages of oil and gas reservoirs. A shape factor parameter is introduced to estimate the changes in the geometry of the borehole as a result of shear failure. A carbonate reservoir from the south of Iran with a solid production history is used to show the application of the developed methodology. Deriving mathematical equations for determination of the shape factor based on different failure criteria indicate that the effect of the intermediate principal stress should be taken into account to achieve an accurate result. However, it should be noticed that the methodology presented can only be used when geomechanical parameters are accurately estimated prior to the production stage when using wells and field data. 展开更多
关键词 Sand production Shape parameter Failure criteria Carbonate reservoir Analytical solution
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SEARCH FOR TRANSLESION SYNTHESIS MODULATORS FROM NATURAL PRODUCTS:ANALYTICAL DEVELOPMENT, VALIDATION AND APPLICATIONS
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作者 Amandine Nachtergael Déborah Lanterbecq +2 位作者 Martine Gadenne Alexandra Belayew Pierre Duez 《World Journal of Traditional Chinese Medicine》 2015年第4期99-99,共1页
DNA translesion synthesis(TLS)is a DNA damage tolerance mechanism that relies on a series of specialized DNA polymerases able to bypass a lesion on a DNA template strand during replication or post-repair synthesis.TLS... DNA translesion synthesis(TLS)is a DNA damage tolerance mechanism that relies on a series of specialized DNA polymerases able to bypass a lesion on a DNA template strand during replication or post-repair synthesis.TLS DNA polymerases pursue replication by inserting a base opposite this lesion,correctly or incorrectly 展开更多
关键词 DNA SEARCH FOR TRANSLESION SYNTHESIS MODULATORS FROM NATURAL productS:ANALYTICAL DEVELOPMENT VALIDATION AND APPLICATIONS
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APPLYING NOVEL ANALYTICAL TECHNOLOGIES TO FACE NATURAL PRODUCTS CHALLENGES
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作者 Kate Yu Lirui Qiao Jing Huang 《World Journal of Traditional Chinese Medicine》 2015年第4期73-74,共2页
Herbal medicines have been used since ancient times for thetreatment of many diseases and illnesses.Modern chromatographic and spectroscopic techniques have had a tremendous impact on the analysis of herbal medicine t... Herbal medicines have been used since ancient times for thetreatment of many diseases and illnesses.Modern chromatographic and spectroscopic techniques have had a tremendous impact on the analysis of herbal medicine to facilitate research as well as enhancement of safety and efficacy of commercial products.Today,with the 展开更多
关键词 APPLYING NOVEL ANALYTICAL TECHNOLOGIES TO FACE NATURAL productS CHALLENGES
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