The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Thus, the ident...The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines (RILs) into two sub-populations: one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two (qFT-B1 and qFT-H) in RILs with the E1 allele and one (qFT-J-2) in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.展开更多
Some key techniques for the development of web-based product data management (PDM) system are introduced. The four-tiered B/S architecture of a PDM system-BITPDM is introduced first, followed by its design and imple...Some key techniques for the development of web-based product data management (PDM) system are introduced. The four-tiered B/S architecture of a PDM system-BITPDM is introduced first, followed by its design and implementation, including virtual data vault, flexible coding system, document management, product structure and configuration management, workflow/process and product maturity management. BITPDM can facilitate the activities from new product introduction phase to manufacturing, and manage the product data andtheir dynamic changing history. Based on Microsoft. NET, XML, web service and SOAP techniques, BITPDM realizes the integration and efficient management of product information.展开更多
基金partially supported by the National Natural Science Foundation of China (31430065, 31571686, 31201222 and 31371643)the Open Foundation of the Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences+5 种基金the “Hundred Talents” Program of the Chinese Academy of Sciencesthe Strategic Action Plan for Science and Technology Innovation of the Chinese Academy of Sciences (XDA08030108)the Natural Science Foundation of Heilongjiang Province, China (ZD201001, JC201313)the Research and Development of Applied Technology Project, Harbin, China (2014RFQYJ055)the Scientific Research Foundation for Returned Chinese Scholars of Heilongjiang Province, China (LC201417)the Science Foundation for Creative Research Talents of Harbin Science and Technology Bureau, China (2014RFQYJ046)
文摘The adaptability of soybean to be grown at a wide range of latitudes is attributed to natural variation in the major genes and quantitative trait loci (QTLs) that control flowering time and maturity. Thus, the identification of genes controlling flowering time and maturity and the understanding of their molecular basis are critical for improving soybean productivity. However, due to the great effect of the major maturity gene E1 on flowering time, it is difficult to detect other small-effect QTLs. In this study, aiming to reduce the effect of the QTL, associated with the E1 gene, on the detection of other QTLs, we divided a population of 96 recombinant inbred lines (RILs) into two sub-populations: one with the E1 allele and another with the elns allele. Compared with the results of using all 96 recombinant inbred lines, additional QTLs for flowering time were identified in the sub-populations, two (qFT-B1 and qFT-H) in RILs with the E1 allele and one (qFT-J-2) in the RILs with the elnl allele, respectively. The three QTLs, qFT-B1, qFT-H and qFT-J-2 were true QTLs and played an important role in the regulation of growth period. Our data provides valuable information for the genetic mapping and gene cloning of traits controlling flowering time and maturity and will help a better understanding of the mechanism of photoperiod-regulated flowering and molecular breeding in soybean.
文摘Some key techniques for the development of web-based product data management (PDM) system are introduced. The four-tiered B/S architecture of a PDM system-BITPDM is introduced first, followed by its design and implementation, including virtual data vault, flexible coding system, document management, product structure and configuration management, workflow/process and product maturity management. BITPDM can facilitate the activities from new product introduction phase to manufacturing, and manage the product data andtheir dynamic changing history. Based on Microsoft. NET, XML, web service and SOAP techniques, BITPDM realizes the integration and efficient management of product information.