Vertically exploring the characteristics of the typhoon boundary layer(TBL) plays an important role in recognizing typhoon structure. The boundary layer radial direction and tangential wind characteristics of Typhoo...Vertically exploring the characteristics of the typhoon boundary layer(TBL) plays an important role in recognizing typhoon structure. The boundary layer radial direction and tangential wind characteristics of Typhoon Usagi based on the observational data of three boundary layer wind profiler stations along the route of Typhoon Usagi(No. 1319) and by combining with sounding data. The results show that:(1) maximum tangential wind appears in the vicinity of the eye area of Usagi, and it basically maintains a height of around 1 800 m when Usagi keeps a strong typhoon level, with the rapidly decreasing strength of Usagi after it lands, the speed of the maximum tangential wind and its vertical range both decrease;(2) the height of the maximum tangential wind is close to that of the inflow layer top of the typhoon, and is greater than that of the boundary layer estimated on the basis of Richardson number or potential temperature gradient, while the height of mixed layer judged on the basis of the signal-to-noise ratio(SNR) or its gradient is usually low;(3) the the boundary layer height can reach higher than2 100 m before Usagi lands. When the typhoon level or above is achieved, the boundary layer height observed by various stations does not change much, basically staying at between 1 200 and 1 600 m. With the decreasing strength of Usagi after its landfall, the boundary layer height rapidly drops.展开更多
Many magnetohydrodynamic stability analyses require generation of a set of equilibria with a fixed safety factor q-profile while varying other plasma parameters.A neural network(NN)-based approach is investigated that...Many magnetohydrodynamic stability analyses require generation of a set of equilibria with a fixed safety factor q-profile while varying other plasma parameters.A neural network(NN)-based approach is investigated that facilitates such a process.Both multilayer perceptron(MLP)-based NN and convolutional neural network(CNN)models are trained to map the q-profile to the plasma current density J-profile,and vice versa,while satisfying the Grad–Shafranov radial force balance constraint.When the initial target models are trained,using a database of semianalytically constructed numerical equilibria,an initial CNN with one convolutional layer is found to perform better than an initial MLP model.In particular,a trained initial CNN model can also predict the q-or J-profile for experimental tokamak equilibria.The performance of both initial target models is further improved by fine-tuning the training database,i.e.by adding realistic experimental equilibria with Gaussian noise.The fine-tuned target models,referred to as fine-tuned MLP and fine-tuned CNN,well reproduce the target q-or J-profile across multiple tokamak devices.As an important application,these NN-based equilibrium profile convertors can be utilized to provide a good initial guess for iterative equilibrium solvers,where the desired input quantity is the safety factor instead of the plasma current density.展开更多
Purpose:This meta-analytical study aimed to explore the effects of resistance training(RT) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females.Methods:A systematic search was p...Purpose:This meta-analytical study aimed to explore the effects of resistance training(RT) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females.Methods:A systematic search was performed for randomized controlled trials in PubMed,Scopus,Web of Science,and SciELO.Randomized controlled trials with postmenopausal and older females that compared RT effects on body adiposity,metabolic risk,and inflammation with a control group(CG) were included.Independent reviewers selected the studies,extracted the data,and performed the risk of bias and certainty of the evidence(Grading of Recommendations,Assessment,Development,and Evaluation(GRADE)) evaluations.Total body and abdominal adiposity,blood lipids,glucose,and C-reactive protein were included for meta-analysis.A random-effects model,standardized mean difference(Hedges’ g),and 95% confidence interval(95%CI) were used for meta-analysis.Results:Twenty randomized controlled trials(overall risk of bias:some concerns;GRADE:low to very low) with overweight/obese postmenopausal and older females were included.RT groups were divided into low-volume RT(LVRT,~44 sets/week) and high-volume RT(HVRT,~77 sets/week).Both RT groups presented improved body adiposity,metabolic risk,and inflammation when compared to CG.However,HVRT demonstrated higher effect sizes than LVRT for glucose(HVRT=-1.19;95%CI:-1.63 to-0.74;LVRT=-0.78;95%CI:-1.15 to-0.41) and C-reactive protein(HVRT=-1.00;95%CI:-1.32 to-0.67;LVRT=-0.34;95%CI,-0.63 to-0.04)) when compared to CG.Conclusion:Compared to CG,HVRT protocols elicit greater improvements in metabolic risk and inflammation outcomes than LVRT in overweight/obese postmenopausal and older females.展开更多
Recently,the application of Bayesian updating to predict excavation-induced deformation has proven successful and improved prediction accuracy significantly.However,updating the ground settlement profile,which is cruc...Recently,the application of Bayesian updating to predict excavation-induced deformation has proven successful and improved prediction accuracy significantly.However,updating the ground settlement profile,which is crucial for determining potential damage to nearby infrastructures,has received limited attention.To address this,this paper proposes a physics-guided simplified model combined with a Bayesian updating framework to accurately predict the ground settlement profile.The advantage of this model is that it eliminates the need for complex finite element modeling and makes the updating framework user-friendly.Furthermore,the model is physically interpretable,which can provide valuable references for construction adjustments.The effectiveness of the proposed method is demonstrated through two field case studies,showing that it can yield satisfactory predictions for the settlement profile.展开更多
Wildfires are complex natural phenomena that exert significant impacts on landscapes,societies,and economies.Understanding the concept of resilience is crucial in mitigating its possible negative impacts,as it involve...Wildfires are complex natural phenomena that exert significant impacts on landscapes,societies,and economies.Understanding the concept of resilience is crucial in mitigating its possible negative impacts,as it involves preparing for,responding to,and recovering from wildfires.This research aims to demonstrate the utility of in situ soil profile description in assessing land use resilience using an Analytic Hierarchy Process(AHP)through an expert panel survey.The study examines a catchment located in the Balearic Islands,considering two fire occurrences(once and twice),comparing abandoned agricultural terraces and natural hillslopes.The results demonstrated that the priority ranking of variables to assess soil profile resilience against wildfires,determined by a panel of 10 experts,identified horizon depth(25.1%),slope inclination(21.5%),and hydrological connectivity(16.6%)as the most crucial factors.Other variables,such as number and size of roots,structure of pedal soil material,size class structure,and rock fragments,also contributed to resilience but to a lesser extent,with scores ranging from 5.7%to 9.6%.Analyzing the priorities established by the experts using AHP,the results showed that the least resilient soil horizon was H1 of the control hillslope,especially under high and low connectivity processes,which aligned with the loss of superficial soil horizons after one and two wildfires.Hillslopes showed greater changes in resilience after occurring wildfires compared to terraces,with the most significant alterations occurring after the second wildfire event.This study addresses a significant knowledge gap in the field by highlighting the interconnectedness of wildfires,resilience,and land use,providing insights into land management strategies for wildfire-prone regions.展开更多
Sweet potato leaf tips have high nutritional value,and exploring the differences in the metabolic profiles of leaf tips among different sweet potato varieties can provide information to improve their qualities.In this...Sweet potato leaf tips have high nutritional value,and exploring the differences in the metabolic profiles of leaf tips among different sweet potato varieties can provide information to improve their qualities.In this study,a UPLC-Q-Exactive Orbitrap/MS-based untargeted metabolomics method was used to evaluate the metabolites in leaf tips of 32 sweet potato varieties.Three varieties with distinct overall metabolic profiles(A01,A02,and A03),two varieties with distinct profiles of phenolic acids(A20 and A18),and three varieties with distinct profiles of flavonoids(A05,A12,and A16)were identified.In addition,a total of 163 and 29 differentially expressed metabolites correlated with the color and leaf shape of sweet potato leaf tips,respectively,were identified through morphological characterization.Group comparison analysis of the phenotypic traits and a metabolite-phenotypic trait correlation analysis indicated that the color differences of sweet potato leaf tips were markedly associated with flavonoids.Also,the level of polyphenols was correlated with the leaf shape of sweet potato leaf tips,with lobed leaf types having higher levels of polyphenols than the entire leaf types.The findings on the metabolic profiles and differentially expressed metabolites associated with the morphology of sweet potato leaf tips can provide useful information for breeding sweet potato varieties with higher nutritional value.展开更多
Fano resonance is a ubiquitous phenomenon, and it is commonly interpreted as a two-channel interference of the discrete and continuous channels. The present work investigates the Fano profile from a perspective of the...Fano resonance is a ubiquitous phenomenon, and it is commonly interpreted as a two-channel interference of the discrete and continuous channels. The present work investigates the Fano profile from a perspective of the temporal evolution of the wave function. By exciting the atom with a δ pulse and calculating the evolution of the wave function, the Fano formula is deduced. The results clearly show that the Fano resonance is of a three-channel interference, which is different from the traditional understanding. The three channels are revealed as the groundcontinuum, ground-discrete-continuum, and a previously unmentioned third channel, i.e., ground-continuumdiscrete-continuum. The present three-channel interpretation can be easily generalized to other physical systems,contributing to a deeper understanding of the Fano profile.展开更多
Ground-based microwave radiometers(MWRs)operating in the K-and V-bands(20–60 GHz)can help us obtain temperature and humidity profiles in the troposphere.Aside from some soundings from local meteorological observatori...Ground-based microwave radiometers(MWRs)operating in the K-and V-bands(20–60 GHz)can help us obtain temperature and humidity profiles in the troposphere.Aside from some soundings from local meteorological observatories,the tropospheric atmosphere over the Tibetan Plateau(TP)has never been continuously observed.As part of the Chinese Second Tibetan Plateau Scientific Expedition and Research Program(STEP),the Tibetan Plateau Atmospheric Profile(TPPROFILE)project aims to construct a comprehensive MWR troposphere observation network to study the synoptic processes and environmental changes on the TP.This initiative has collected three years of data from the MWR network.This paper introduces the data information,the data quality,and data downloading.Some applications of the data obtained from these MWRs were also demonstrated.Our comparisons of MWR against the nearest radiosonde observation demonstrate that the TP-PROFILE MWR system is adequate for monitoring the thermal and moisture variability of the troposphere over the TP.The continuous temperature and moisture profiles derived from the MWR data provide a unique perspective on the evolution of the thermodynamic structure associated with the heating of the TP.The TP-PROFILE project reveals that the low-temporal resolution instruments are prone to large uncertainties in their vapor estimation in the mountain valleys on the TP.展开更多
The coherent muon-to-electron transition(COMET)experiment is a leading experiment for the coherent conversion of μ^(-)N→e^(-)N using a high-intensity pulsed muon beamline,produced using innovative slow-extraction te...The coherent muon-to-electron transition(COMET)experiment is a leading experiment for the coherent conversion of μ^(-)N→e^(-)N using a high-intensity pulsed muon beamline,produced using innovative slow-extraction techniques.Therefore,it is critical to measure the muon beam characteristics.We set up a muon beam monitor(MBM),where scintillating fibers woven in a cross shape were coupled to silicon photomultipliers to measure the spatial profile and timing structure of the extracted muon beam for the COMET.The MBM detector was tested successfully with a proton beamline at the China Spallation Neutron Source and took data with good performance in the commissioning run.The development of the MBM,including its mechanical structure,electronic readout,and beam measurement results,are discussed.展开更多
2D profile lines play a critical role in cost-effectively evaluating rock joint properties and shear strength.However,the interval(DIL)between these lines significantly impacts roughness and shear strength assessments...2D profile lines play a critical role in cost-effectively evaluating rock joint properties and shear strength.However,the interval(DIL)between these lines significantly impacts roughness and shear strength assessments.A detailed study of 45 joint samples using four statistical measures across 500 different DIL values identified a clear line interval effect with two stages:stable and fluctuation-discrete.Further statistical analysis showed a linear relationship between the error bounds of four parameters,shear strength evaluation,and their corresponding maximum DIL values,where the gradient k of this linear relationship was influenced by the basic friction angle and normal stress.Accounting for these factors,lower-limit linear models were employed to determine the optimal DIL values that met error tolerances(1%–10%)for all metrics and shear strength.The study also explored the consistent size effect on joints regardless of DIL changes,revealing three types of size effects based on morphological heterogeneity.Notably,larger joints required generally higher DIL to maintain the predefined error limits,suggesting an increased interval for large joint analyses.Consequently,this research provides a basis for determining the optimal DIL,improving accuracy in 2D profile line assessments of joint characteristics.展开更多
Achieving increasingly finely targeted drug delivery to organs,tissues,cells,and even to intracellular biomacromolecules is one of the core goals of nanomedicines.As the delivery destination is refined to cellular and...Achieving increasingly finely targeted drug delivery to organs,tissues,cells,and even to intracellular biomacromolecules is one of the core goals of nanomedicines.As the delivery destination is refined to cellular and subcellular targets,it is essential to explore the delivery of nanomedicines at the molecular level.However,due to the lack of technical methods,the molecular mechanism of the intracellular delivery of nanomedicines remains unclear to date.Here,we develop an enzyme-induced proximity labeling technology in nanoparticles(nano-EPL)for the real-time monitoring of proteins that interact with intracellular nanomedicines.Poly(lactic-co-glycolic acid)nanoparticles coupled with horseradish peroxidase(HRP)were fabricated as a model(HRP(+)-PNPs)to evaluate the molecular mechanism of nano delivery in macrophages.By adding the labeling probe biotin-phenol and the catalytic substrate H_(2)O_(2)at different time points in cellular delivery,nano-EPL technology was validated for the real-time in situ labeling of proteins interacting with nanoparticles.Nano-EPL achieves the dynamic molecular profiling of 740 proteins to map the intracellular delivery of HRP(+)-PNPs in macrophages over time.Based on dynamic clustering analysis of these proteins,we further discovered that different organelles,including endosomes,lysosomes,the endoplasmic reticulum,and the Golgi apparatus,are involved in delivery with distinct participation timelines.More importantly,the engagement of these organelles differentially affects the drug delivery efficiency,reflecting the spatial–temporal heterogeneity of nano delivery in cells.In summary,these findings highlight a significant methodological advance toward understanding the molecular mechanisms involved in the intracellular delivery of nanomedicines.展开更多
Understanding the vertical distribution of ozone is crucial when assessing both its horizontal and vertical transport,as well as when analyzing the physical and chemical properties of the atmosphere.One of the most ef...Understanding the vertical distribution of ozone is crucial when assessing both its horizontal and vertical transport,as well as when analyzing the physical and chemical properties of the atmosphere.One of the most effective ways to obtain high spatial resolution ozone profiles is through satellite observations.The Environmental Trace Gases Monitoring Instrument(EMI)deployed on the Gaofen-5 satellite is the first Chinese ultraviolet-visible hyperspectral spectrometer.However,retrieving ozone profiles using backscattered radiance values measured by the EMI is challenging due to unavailable measurement errors and a low signal-to-noise ratio.The algorithm developed for the Tropospheric Monitoring Instrument did not allow us to retrieve 87%of the EMI pixels.Therefore,we developed an algorithm specific to the characteristics of the EMI.The fitting residuals are smaller than 0.3%in most regions.The retrieved ozone profiles were in good agreement with ozonesonde data,with maximum mean biases of 20%at five latitude bands.By applying EMI averaging kernels to the ozonesonde profiles,the integrated stratospheric column ozone and tropospheric column ozone also showed excellent agreement with ozonesonde data,The lower layers(0-7.5 km)of the EMI ozone profiles reflected the seasonal variation in surface ozone derived from the China National Environmental Monitoring Center(CNEMC).However,the upper layers(9.7-16.7 km)of the ozone profiles show different trends,with the ozone peak occurring at an altitude of 9.7-16.7 km in March,2019.A stratospheric intrusion event in central China from August 11 to 15,2019,is captured using the EMI ozone profiles,potential vorticity data,and relative humidity data.The increase in the CNEMC ozone co ncentration showed that downward transport enhanced surface ozone pollution.展开更多
Reconstruction of plasma equilibrium plays an important role in the analysis and simulation of plasma experiments. The kinetic equilibrium reconstruction with pressure and edge current constraints has been employed on...Reconstruction of plasma equilibrium plays an important role in the analysis and simulation of plasma experiments. The kinetic equilibrium reconstruction with pressure and edge current constraints has been employed on EAST tokamak. However, the internal safety factor(q) profile is not accurate. This paper proposes a new way of incorporating q profile constraints into kinetic equilibrium reconstruction. The q profile is yielded from the Polarimeter Interferometer(POINT)reconstruction. Virtual probes containing information on q profile constraints are added to inputs of the kinetic equilibrium reconstruction program to obtain the final equilibrium. The new equilibrium produces a more accurate internal q profile. This improved method would help analyze EAST experiments.展开更多
Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory capabilities.Transient receptor potential(TRP)channels,which constitute a vast ion chann...Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory capabilities.Transient receptor potential(TRP)channels,which constitute a vast ion channel family,play pivotal roles in sensing the external environment and maintaining internal homeostasis in insects.TRP channels have been widely investigated for their critical roles in regulating various insect behaviors in recent years.In this study,we identified 15 TRP gene loci encoding 26 transcripts in the genome of S.frugiperda and analyzed their expression profiles at different developmental stages.The results revealed that S.frugiperda possesses four TRPC genes,six TRPA genes,one TRPM gene,two TRPV genes,one TRPN gene,and one TRPML gene,while a canonical TRPP is absent.Moreover,the SfruTRPA1 was functionally characterized using the Xenopus oocyte expression system.The results showed that SfruTRPA1 is activated by temperature increases from 20 to 45℃,and there is no significant desensitization after repeated stimuli within the same temperature range.Additionally,SfruTRPA1 is activated by certain natural chemicals,including allyl isothiocyanate(AITC)and cinnamaldehyde(CA).These findings provide valuable insights to the TRP genes in S.frugiperda.展开更多
Objective To investigate changes in the urinary metabolite profiles of children exposed to polycyclic aromatic hydrocarbons(PAHs)during critical brain development and explore their potential link with the intestinal m...Objective To investigate changes in the urinary metabolite profiles of children exposed to polycyclic aromatic hydrocarbons(PAHs)during critical brain development and explore their potential link with the intestinal microbiota.Methods Liquid chromatography-tandem mass spectrometry was used to determine ten hydroxyl metabolites of PAHs(OH-PAHs)in 36-month-old children.Subsequently,37 children were categorized into low-and high-exposure groups based on the sum of the ten OH-PAHs.Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to identify non-targeted metabolites in the urine samples.Furthermore,fecal flora abundance was assessed by 16S rRNA gene sequencing using Illumina MiSeq.Results The concentrations of 21 metabolites were significantly higher in the high exposure group than in the low exposure group(variable importance for projection>1,P<0.05).Most of these metabolites were positively correlated with the hydroxyl metabolites of naphthalene,fluorine,and phenanthrene(r=0.336–0.531).The identified differential metabolites primarily belonged to pathways associated with inflammation or proinflammatory states,including amino acid,lipid,and nucleotide metabolism.Additionally,these distinct metabolites were significantly associated with specific intestinal flora abundances(r=0.34–0.55),which were mainly involved in neurodevelopment.Conclusion Higher PAH exposure in young children affected metabolic homeostasis,particularly that of certain gut microbiota-derived metabolites.Further investigation is needed to explore the potential influence of PAHs on the gut microbiota and their possible association with neurodevelopmental outcomes.展开更多
This article investigates a multi-circular path-following formation control with reinforced transient profiles for nonholonomic vehicles connected by a digraph.A multi-circular formation controller endowed with the fe...This article investigates a multi-circular path-following formation control with reinforced transient profiles for nonholonomic vehicles connected by a digraph.A multi-circular formation controller endowed with the feature of spatial-temporal decoupling is devised for a group of vehicles guided by a virtual leader evolving along an implicit path,which allows for a circumnavigation on multiple circles with an anticipant angular spacing.In addition,notice that it typically imposes a stringent time constraint on time-sensitive enclosing scenarios,hence an improved prescribed performance control(IPPC)using novel tighter behavior boundaries is presented to enhance transient capabilities with an ensured appointed-time convergence free from any overshoots.The significant merits are that coordinated circumnavigation along different circles can be realized via executing geometric and dynamic assignments independently with modified transient profiles.Furthermore,all variables existing in the entire system are analyzed to be convergent.Simulation and experimental results are provided to validate the utility of suggested solution.展开更多
Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed...Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.展开更多
Accurate simulation of the evolution of freak waves by the wave phase focusing method requires accurate linear and nonlinear properties,especially in deep-water conditions.In this paper,we analyze the ability to simul...Accurate simulation of the evolution of freak waves by the wave phase focusing method requires accurate linear and nonlinear properties,especially in deep-water conditions.In this paper,we analyze the ability to simulate deep-water focused waves of a two-layer Boussinesq-type(BT)model,which has been shown to have excellent linear and nonlinear performance.To further improve the numerical accuracy and stability,the internal wavegenerated method is introduced into the two-layer Boussinesq-type model.Firstly,the sensitivity of the numerical results to the grid resolution is analyzed to verify the convergence of the model;secondly,the focused wave propagating in two opposite directions is simulated to prove the symmetry of the numerical results and the feasibility of the internal wave-generated method;thirdly,the limiting focused wave condition is simulated to compare and analyze the wave surface and the horizontal velocity of the profile at the focusing position,which is in good agreement with the measured values.Meanwhile the simulation of focused waves in very deep waters agrees well with the measured values,which further demonstrates the capability of the two-layer BT model in simulating focused waves in deep waters.展开更多
Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens...Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.展开更多
基金The National Natural Science Foundation of China under contract Nos 41305029 and 41475014the Science and Technology Planning Project of Guangdong Province of China under contract No.2013B020200013+1 种基金the Guangzhou Municipal Science and Technology Planning Project of China under contract No.201604020069the Meteorological Key Technology Integration and Application Projects of China Meteorological Administration under contract No.CMAGJ2015M42
文摘Vertically exploring the characteristics of the typhoon boundary layer(TBL) plays an important role in recognizing typhoon structure. The boundary layer radial direction and tangential wind characteristics of Typhoon Usagi based on the observational data of three boundary layer wind profiler stations along the route of Typhoon Usagi(No. 1319) and by combining with sounding data. The results show that:(1) maximum tangential wind appears in the vicinity of the eye area of Usagi, and it basically maintains a height of around 1 800 m when Usagi keeps a strong typhoon level, with the rapidly decreasing strength of Usagi after it lands, the speed of the maximum tangential wind and its vertical range both decrease;(2) the height of the maximum tangential wind is close to that of the inflow layer top of the typhoon, and is greater than that of the boundary layer estimated on the basis of Richardson number or potential temperature gradient, while the height of mixed layer judged on the basis of the signal-to-noise ratio(SNR) or its gradient is usually low;(3) the the boundary layer height can reach higher than2 100 m before Usagi lands. When the typhoon level or above is achieved, the boundary layer height observed by various stations does not change much, basically staying at between 1 200 and 1 600 m. With the decreasing strength of Usagi after its landfall, the boundary layer height rapidly drops.
基金supported by National Natural Science Foundation of China (Nos. 12205033, 12105317, 11905022 and 11975062)Dalian Youth Science and Technology Project (No. 2022RQ039)+1 种基金the Fundamental Research Funds for the Central Universities (No. 3132023192)the Young Scientists Fund of the Natural Science Foundation of Sichuan Province (No. 2023NSFSC1291)
文摘Many magnetohydrodynamic stability analyses require generation of a set of equilibria with a fixed safety factor q-profile while varying other plasma parameters.A neural network(NN)-based approach is investigated that facilitates such a process.Both multilayer perceptron(MLP)-based NN and convolutional neural network(CNN)models are trained to map the q-profile to the plasma current density J-profile,and vice versa,while satisfying the Grad–Shafranov radial force balance constraint.When the initial target models are trained,using a database of semianalytically constructed numerical equilibria,an initial CNN with one convolutional layer is found to perform better than an initial MLP model.In particular,a trained initial CNN model can also predict the q-or J-profile for experimental tokamak equilibria.The performance of both initial target models is further improved by fine-tuning the training database,i.e.by adding realistic experimental equilibria with Gaussian noise.The fine-tuned target models,referred to as fine-tuned MLP and fine-tuned CNN,well reproduce the target q-or J-profile across multiple tokamak devices.As an important application,these NN-based equilibrium profile convertors can be utilized to provide a good initial guess for iterative equilibrium solvers,where the desired input quantity is the safety factor instead of the plasma current density.
基金supported by the Minas Gerais State University (UEMG/Brazil)a Research Productivity Scholarship Program (UEMG-PQ08/2021)+1 种基金a doctorate scholarship from the National Council of Technological and Scientific Development (CNPq/Brazil-Process140473/2020-3)a doctorate scholarship fromthe Coordination of Improvement of Higher Education Personnel (CAPES/Brazil-Code 001)。
文摘Purpose:This meta-analytical study aimed to explore the effects of resistance training(RT) volume on body adiposity,metabolic risk,and inflammation in postmenopausal and older females.Methods:A systematic search was performed for randomized controlled trials in PubMed,Scopus,Web of Science,and SciELO.Randomized controlled trials with postmenopausal and older females that compared RT effects on body adiposity,metabolic risk,and inflammation with a control group(CG) were included.Independent reviewers selected the studies,extracted the data,and performed the risk of bias and certainty of the evidence(Grading of Recommendations,Assessment,Development,and Evaluation(GRADE)) evaluations.Total body and abdominal adiposity,blood lipids,glucose,and C-reactive protein were included for meta-analysis.A random-effects model,standardized mean difference(Hedges’ g),and 95% confidence interval(95%CI) were used for meta-analysis.Results:Twenty randomized controlled trials(overall risk of bias:some concerns;GRADE:low to very low) with overweight/obese postmenopausal and older females were included.RT groups were divided into low-volume RT(LVRT,~44 sets/week) and high-volume RT(HVRT,~77 sets/week).Both RT groups presented improved body adiposity,metabolic risk,and inflammation when compared to CG.However,HVRT demonstrated higher effect sizes than LVRT for glucose(HVRT=-1.19;95%CI:-1.63 to-0.74;LVRT=-0.78;95%CI:-1.15 to-0.41) and C-reactive protein(HVRT=-1.00;95%CI:-1.32 to-0.67;LVRT=-0.34;95%CI,-0.63 to-0.04)) when compared to CG.Conclusion:Compared to CG,HVRT protocols elicit greater improvements in metabolic risk and inflammation outcomes than LVRT in overweight/obese postmenopausal and older females.
基金the financial support from the Guangdong Provincial Department of Science and Technology(Grant No.2022A0505030019)the Science and Technology Development Fund,Macao SAR,China(File Nos.0056/2023/RIB2 and SKL-IOTSC-2021-2023).
文摘Recently,the application of Bayesian updating to predict excavation-induced deformation has proven successful and improved prediction accuracy significantly.However,updating the ground settlement profile,which is crucial for determining potential damage to nearby infrastructures,has received limited attention.To address this,this paper proposes a physics-guided simplified model combined with a Bayesian updating framework to accurately predict the ground settlement profile.The advantage of this model is that it eliminates the need for complex finite element modeling and makes the updating framework user-friendly.Furthermore,the model is physically interpretable,which can provide valuable references for construction adjustments.The effectiveness of the proposed method is demonstrated through two field case studies,showing that it can yield satisfactory predictions for the settlement profile.
基金supported by the research project CGL2017-88200-R,titled"Functional Hydrological and Sediment Connectivity in Mediterranean Catchments:Global Change Scenarios–MEDhyCON_2,"funded by the Spanish Ministry of Science,Innovation,and Universities,the Spanish Agency of Research (AEI)the European Regional Development Funds (ERDF)funded by COST Action (grant no. CA18135),supported by COST (European Cooperation in Science and Technology),during a Short-Term Scientific Mission (STSM) conducted by Jesús Rodrigo-Comino
文摘Wildfires are complex natural phenomena that exert significant impacts on landscapes,societies,and economies.Understanding the concept of resilience is crucial in mitigating its possible negative impacts,as it involves preparing for,responding to,and recovering from wildfires.This research aims to demonstrate the utility of in situ soil profile description in assessing land use resilience using an Analytic Hierarchy Process(AHP)through an expert panel survey.The study examines a catchment located in the Balearic Islands,considering two fire occurrences(once and twice),comparing abandoned agricultural terraces and natural hillslopes.The results demonstrated that the priority ranking of variables to assess soil profile resilience against wildfires,determined by a panel of 10 experts,identified horizon depth(25.1%),slope inclination(21.5%),and hydrological connectivity(16.6%)as the most crucial factors.Other variables,such as number and size of roots,structure of pedal soil material,size class structure,and rock fragments,also contributed to resilience but to a lesser extent,with scores ranging from 5.7%to 9.6%.Analyzing the priorities established by the experts using AHP,the results showed that the least resilient soil horizon was H1 of the control hillslope,especially under high and low connectivity processes,which aligned with the loss of superficial soil horizons after one and two wildfires.Hillslopes showed greater changes in resilience after occurring wildfires compared to terraces,with the most significant alterations occurring after the second wildfire event.This study addresses a significant knowledge gap in the field by highlighting the interconnectedness of wildfires,resilience,and land use,providing insights into land management strategies for wildfire-prone regions.
基金This work was supported by grants from the construction and operation of the Food Nutrition and Health Research Center of Guangdong Academy of Agricultural Sciences,China(XTXM 202205)the earmarked fund for CARS-10Sweetpotato,and the Guangdong Modern Agro-industry Technology Research System,China(2022KJ111).
文摘Sweet potato leaf tips have high nutritional value,and exploring the differences in the metabolic profiles of leaf tips among different sweet potato varieties can provide information to improve their qualities.In this study,a UPLC-Q-Exactive Orbitrap/MS-based untargeted metabolomics method was used to evaluate the metabolites in leaf tips of 32 sweet potato varieties.Three varieties with distinct overall metabolic profiles(A01,A02,and A03),two varieties with distinct profiles of phenolic acids(A20 and A18),and three varieties with distinct profiles of flavonoids(A05,A12,and A16)were identified.In addition,a total of 163 and 29 differentially expressed metabolites correlated with the color and leaf shape of sweet potato leaf tips,respectively,were identified through morphological characterization.Group comparison analysis of the phenotypic traits and a metabolite-phenotypic trait correlation analysis indicated that the color differences of sweet potato leaf tips were markedly associated with flavonoids.Also,the level of polyphenols was correlated with the leaf shape of sweet potato leaf tips,with lobed leaf types having higher levels of polyphenols than the entire leaf types.The findings on the metabolic profiles and differentially expressed metabolites associated with the morphology of sweet potato leaf tips can provide useful information for breeding sweet potato varieties with higher nutritional value.
基金supported by the National Natural Science Foundation of China (Grant No. 12334010)。
文摘Fano resonance is a ubiquitous phenomenon, and it is commonly interpreted as a two-channel interference of the discrete and continuous channels. The present work investigates the Fano profile from a perspective of the temporal evolution of the wave function. By exciting the atom with a δ pulse and calculating the evolution of the wave function, the Fano formula is deduced. The results clearly show that the Fano resonance is of a three-channel interference, which is different from the traditional understanding. The three channels are revealed as the groundcontinuum, ground-discrete-continuum, and a previously unmentioned third channel, i.e., ground-continuumdiscrete-continuum. The present three-channel interpretation can be easily generalized to other physical systems,contributing to a deeper understanding of the Fano profile.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(Grant Nos.2019QZKK0103 and 2019QZKK0105)the National Natural Science Foundation of China(Grant Nos.41975009,42230610,41840650 and U2242208)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Wang Binbin,2022069).
文摘Ground-based microwave radiometers(MWRs)operating in the K-and V-bands(20–60 GHz)can help us obtain temperature and humidity profiles in the troposphere.Aside from some soundings from local meteorological observatories,the tropospheric atmosphere over the Tibetan Plateau(TP)has never been continuously observed.As part of the Chinese Second Tibetan Plateau Scientific Expedition and Research Program(STEP),the Tibetan Plateau Atmospheric Profile(TPPROFILE)project aims to construct a comprehensive MWR troposphere observation network to study the synoptic processes and environmental changes on the TP.This initiative has collected three years of data from the MWR network.This paper introduces the data information,the data quality,and data downloading.Some applications of the data obtained from these MWRs were also demonstrated.Our comparisons of MWR against the nearest radiosonde observation demonstrate that the TP-PROFILE MWR system is adequate for monitoring the thermal and moisture variability of the troposphere over the TP.The continuous temperature and moisture profiles derived from the MWR data provide a unique perspective on the evolution of the thermodynamic structure associated with the heating of the TP.The TP-PROFILE project reveals that the low-temporal resolution instruments are prone to large uncertainties in their vapor estimation in the mountain valleys on the TP.
基金supported in part by Fundamental Research Funds for the Central Universities(23xkjc017)at Sun Yat-sen Universitythe National Natural Science Foundation of China(No.12075326)JSPS KAKENHI(No.22H00139)。
文摘The coherent muon-to-electron transition(COMET)experiment is a leading experiment for the coherent conversion of μ^(-)N→e^(-)N using a high-intensity pulsed muon beamline,produced using innovative slow-extraction techniques.Therefore,it is critical to measure the muon beam characteristics.We set up a muon beam monitor(MBM),where scintillating fibers woven in a cross shape were coupled to silicon photomultipliers to measure the spatial profile and timing structure of the extracted muon beam for the COMET.The MBM detector was tested successfully with a proton beamline at the China Spallation Neutron Source and took data with good performance in the commissioning run.The development of the MBM,including its mechanical structure,electronic readout,and beam measurement results,are discussed.
基金the National Natural Science Foundation of China(Nos.42002275 and 52325905)the Natural Science Foundation of Zhejiang Province(No.LQ24D020012)+2 种基金the Open Research Fund of State Key Laboratory of Geomechanics and Geotechnical Engineering(No.SKLGME023007)Open Fund of Badong National Observation and Research Station of Geohazards(No.BNORSG202308)the Shaoxing Science and Technology Plan Project(No.2022A13003).
文摘2D profile lines play a critical role in cost-effectively evaluating rock joint properties and shear strength.However,the interval(DIL)between these lines significantly impacts roughness and shear strength assessments.A detailed study of 45 joint samples using four statistical measures across 500 different DIL values identified a clear line interval effect with two stages:stable and fluctuation-discrete.Further statistical analysis showed a linear relationship between the error bounds of four parameters,shear strength evaluation,and their corresponding maximum DIL values,where the gradient k of this linear relationship was influenced by the basic friction angle and normal stress.Accounting for these factors,lower-limit linear models were employed to determine the optimal DIL values that met error tolerances(1%–10%)for all metrics and shear strength.The study also explored the consistent size effect on joints regardless of DIL changes,revealing three types of size effects based on morphological heterogeneity.Notably,larger joints required generally higher DIL to maintain the predefined error limits,suggesting an increased interval for large joint analyses.Consequently,this research provides a basis for determining the optimal DIL,improving accuracy in 2D profile line assessments of joint characteristics.
基金supported by Natural Science Foundation of Beijing Municipality(L212013)National Key Research and Development Program of China(No.2022YFA1206104)+2 种基金AI+Health Collaborative Innovation Cultivation Project(Z211100003521002)National Natural Science Foundation of China(81971718,82073786,81872809,U20A20412,81821004)Beijing Natural Science Foundation(7222020).
文摘Achieving increasingly finely targeted drug delivery to organs,tissues,cells,and even to intracellular biomacromolecules is one of the core goals of nanomedicines.As the delivery destination is refined to cellular and subcellular targets,it is essential to explore the delivery of nanomedicines at the molecular level.However,due to the lack of technical methods,the molecular mechanism of the intracellular delivery of nanomedicines remains unclear to date.Here,we develop an enzyme-induced proximity labeling technology in nanoparticles(nano-EPL)for the real-time monitoring of proteins that interact with intracellular nanomedicines.Poly(lactic-co-glycolic acid)nanoparticles coupled with horseradish peroxidase(HRP)were fabricated as a model(HRP(+)-PNPs)to evaluate the molecular mechanism of nano delivery in macrophages.By adding the labeling probe biotin-phenol and the catalytic substrate H_(2)O_(2)at different time points in cellular delivery,nano-EPL technology was validated for the real-time in situ labeling of proteins interacting with nanoparticles.Nano-EPL achieves the dynamic molecular profiling of 740 proteins to map the intracellular delivery of HRP(+)-PNPs in macrophages over time.Based on dynamic clustering analysis of these proteins,we further discovered that different organelles,including endosomes,lysosomes,the endoplasmic reticulum,and the Golgi apparatus,are involved in delivery with distinct participation timelines.More importantly,the engagement of these organelles differentially affects the drug delivery efficiency,reflecting the spatial–temporal heterogeneity of nano delivery in cells.In summary,these findings highlight a significant methodological advance toward understanding the molecular mechanisms involved in the intracellular delivery of nanomedicines.
基金supported by the National Natural Science Foundation of China(42225504 and 41977184)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23020301)+3 种基金the Key Research and Development Project of Anhui Province(202104i07020002)the Major Projects of High Resolution Earth Observation Systems of National Science and Technology(05-Y30B01-9001-19/20-3)the Key Laboratory of Atmospheric Chemistry/China Meteorological Administration(LAC/CMA)(2022B06)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(2021443).
文摘Understanding the vertical distribution of ozone is crucial when assessing both its horizontal and vertical transport,as well as when analyzing the physical and chemical properties of the atmosphere.One of the most effective ways to obtain high spatial resolution ozone profiles is through satellite observations.The Environmental Trace Gases Monitoring Instrument(EMI)deployed on the Gaofen-5 satellite is the first Chinese ultraviolet-visible hyperspectral spectrometer.However,retrieving ozone profiles using backscattered radiance values measured by the EMI is challenging due to unavailable measurement errors and a low signal-to-noise ratio.The algorithm developed for the Tropospheric Monitoring Instrument did not allow us to retrieve 87%of the EMI pixels.Therefore,we developed an algorithm specific to the characteristics of the EMI.The fitting residuals are smaller than 0.3%in most regions.The retrieved ozone profiles were in good agreement with ozonesonde data,with maximum mean biases of 20%at five latitude bands.By applying EMI averaging kernels to the ozonesonde profiles,the integrated stratospheric column ozone and tropospheric column ozone also showed excellent agreement with ozonesonde data,The lower layers(0-7.5 km)of the EMI ozone profiles reflected the seasonal variation in surface ozone derived from the China National Environmental Monitoring Center(CNEMC).However,the upper layers(9.7-16.7 km)of the ozone profiles show different trends,with the ozone peak occurring at an altitude of 9.7-16.7 km in March,2019.A stratospheric intrusion event in central China from August 11 to 15,2019,is captured using the EMI ozone profiles,potential vorticity data,and relative humidity data.The increase in the CNEMC ozone co ncentration showed that downward transport enhanced surface ozone pollution.
基金supported by National Key R&D Program of China(Nos.2019YFE03040004 and 2017YFE0300404)supported by Comprehensive Research Facility for Fusion Technology Program of China(No.2018000052-73-01-001228)。
文摘Reconstruction of plasma equilibrium plays an important role in the analysis and simulation of plasma experiments. The kinetic equilibrium reconstruction with pressure and edge current constraints has been employed on EAST tokamak. However, the internal safety factor(q) profile is not accurate. This paper proposes a new way of incorporating q profile constraints into kinetic equilibrium reconstruction. The q profile is yielded from the Polarimeter Interferometer(POINT)reconstruction. Virtual probes containing information on q profile constraints are added to inputs of the kinetic equilibrium reconstruction program to obtain the final equilibrium. The new equilibrium produces a more accurate internal q profile. This improved method would help analyze EAST experiments.
基金funded by the Shenzhen Science and Technology Program,China(KQTD20180411143628272)the Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District,China(pt202101-02)the National Key R&D Program of China(2022YFE0116500).
文摘Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory capabilities.Transient receptor potential(TRP)channels,which constitute a vast ion channel family,play pivotal roles in sensing the external environment and maintaining internal homeostasis in insects.TRP channels have been widely investigated for their critical roles in regulating various insect behaviors in recent years.In this study,we identified 15 TRP gene loci encoding 26 transcripts in the genome of S.frugiperda and analyzed their expression profiles at different developmental stages.The results revealed that S.frugiperda possesses four TRPC genes,six TRPA genes,one TRPM gene,two TRPV genes,one TRPN gene,and one TRPML gene,while a canonical TRPP is absent.Moreover,the SfruTRPA1 was functionally characterized using the Xenopus oocyte expression system.The results showed that SfruTRPA1 is activated by temperature increases from 20 to 45℃,and there is no significant desensitization after repeated stimuli within the same temperature range.Additionally,SfruTRPA1 is activated by certain natural chemicals,including allyl isothiocyanate(AITC)and cinnamaldehyde(CA).These findings provide valuable insights to the TRP genes in S.frugiperda.
基金partly supported by grants from the National Natural Science Foundation of China(No:81372955)BiosTime Maternal and Infant Nutrition and Health Research Fund(2017FYH008)+1 种基金the Projects of Medical and Health Technology Development Program in Shandong Province(No:2016WS0309)Qingdao Outstanding Health Professional Development Fund(2020-2022,2022-2024)。
文摘Objective To investigate changes in the urinary metabolite profiles of children exposed to polycyclic aromatic hydrocarbons(PAHs)during critical brain development and explore their potential link with the intestinal microbiota.Methods Liquid chromatography-tandem mass spectrometry was used to determine ten hydroxyl metabolites of PAHs(OH-PAHs)in 36-month-old children.Subsequently,37 children were categorized into low-and high-exposure groups based on the sum of the ten OH-PAHs.Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to identify non-targeted metabolites in the urine samples.Furthermore,fecal flora abundance was assessed by 16S rRNA gene sequencing using Illumina MiSeq.Results The concentrations of 21 metabolites were significantly higher in the high exposure group than in the low exposure group(variable importance for projection>1,P<0.05).Most of these metabolites were positively correlated with the hydroxyl metabolites of naphthalene,fluorine,and phenanthrene(r=0.336–0.531).The identified differential metabolites primarily belonged to pathways associated with inflammation or proinflammatory states,including amino acid,lipid,and nucleotide metabolism.Additionally,these distinct metabolites were significantly associated with specific intestinal flora abundances(r=0.34–0.55),which were mainly involved in neurodevelopment.Conclusion Higher PAH exposure in young children affected metabolic homeostasis,particularly that of certain gut microbiota-derived metabolites.Further investigation is needed to explore the potential influence of PAHs on the gut microbiota and their possible association with neurodevelopmental outcomes.
基金supported in part by the National Natural Science Foundation of China under Grant Nos.62173312 and 61803348in part by the National Major Scientific Instruments Development Project under Grant No.61927807+3 种基金in part by the Program for the Innovative Talents of Higher Education Institutions of ShanxiShanxi Province Science Foundation for Excellent Youthsin part by the Shanxi"1331 Project"Key Subjects Construction(1331KSC)in part by Graduate Innovation Project of Shanxi Province under Grant No.2021Y617。
文摘This article investigates a multi-circular path-following formation control with reinforced transient profiles for nonholonomic vehicles connected by a digraph.A multi-circular formation controller endowed with the feature of spatial-temporal decoupling is devised for a group of vehicles guided by a virtual leader evolving along an implicit path,which allows for a circumnavigation on multiple circles with an anticipant angular spacing.In addition,notice that it typically imposes a stringent time constraint on time-sensitive enclosing scenarios,hence an improved prescribed performance control(IPPC)using novel tighter behavior boundaries is presented to enhance transient capabilities with an ensured appointed-time convergence free from any overshoots.The significant merits are that coordinated circumnavigation along different circles can be realized via executing geometric and dynamic assignments independently with modified transient profiles.Furthermore,all variables existing in the entire system are analyzed to be convergent.Simulation and experimental results are provided to validate the utility of suggested solution.
基金partly funded by the Department of Science and Technology Fund for Improvement of S&T Infrastructure (Grant No. SR/FST/LS-I/2018/125)。
文摘Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.
基金The National Natural Science Foundation under contract Nos 52171247,51779022,52071057,and 51709054.
文摘Accurate simulation of the evolution of freak waves by the wave phase focusing method requires accurate linear and nonlinear properties,especially in deep-water conditions.In this paper,we analyze the ability to simulate deep-water focused waves of a two-layer Boussinesq-type(BT)model,which has been shown to have excellent linear and nonlinear performance.To further improve the numerical accuracy and stability,the internal wavegenerated method is introduced into the two-layer Boussinesq-type model.Firstly,the sensitivity of the numerical results to the grid resolution is analyzed to verify the convergence of the model;secondly,the focused wave propagating in two opposite directions is simulated to prove the symmetry of the numerical results and the feasibility of the internal wave-generated method;thirdly,the limiting focused wave condition is simulated to compare and analyze the wave surface and the horizontal velocity of the profile at the focusing position,which is in good agreement with the measured values.Meanwhile the simulation of focused waves in very deep waters agrees well with the measured values,which further demonstrates the capability of the two-layer BT model in simulating focused waves in deep waters.
基金supported by Brain Korea (BK)21 Plus Project (4299990913942)funded by the Korean Government,Koreathe Collabo Project funded by the Ministry of SMEs and Startups (C1016120-01-02)the National Research Foundation of Korea (NRF) (2018007551)。
文摘Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.