THE MIB-1 PROLIFERATION INDEX AND MICROVESSEL COUNT IN EARLY GASTRIC CANCER:ASSOCIATION WITH LYMPH NODE METASTASIS

Objective Tumors with accelerated growth or high malignancy are thought to undergo active angio- genesis. Whereas by far, there is few study concerning the combination of MIB-1 proliferation index (MIB-1 PI) and tumor angiogenesis in carcinomas to show their significance in relate to clinicopathological parameters. In the present study, we evaluated the significance or MIB-1 PI and angiogenesis in early stage of gastric cancer. Our focus was es- pecially on the combination of MIB-1 PI and angiogenesis in relate to lymph node metastasis. Method Specimens from 95 patients with early gastric cancer were studied by means or immunohistochemistry using monoclonal MIB-1 and factor Ⅷ related antigen antibodies. Results The mean MIB-1 PI and microvessel count were 22.9% and 34.7, respectively. The MIB-1 PI did not correlate with microvessel count. Both correlated with depth of tumor invasion, lymphatic vessel invasion and lymph node metastasis. Multivarlate analysis showed that combined high MIB-1 PI/hy- pervascularity, as well as lymphatic vessel invasion and tumor size were independent factors that impact on lymph node metastasis. Conclusion A combination of the high MIB-1 PI/hypervascularity is a factor that related to lymph node metastasis in early gastric cancer.

Comparison of proliferation index between nestin-positive cells and glial fibrillary acidic protein-positive cells in human astrocytic tumors

Multivariate modeling has demonstrated that the proliferation index （PI） of tumor cells is one of the strongest prognostic factors related to the survival of patients with astrocytic tumors. Studies have indicated that the glioma regenerative cells derived from nestin-positive （N^＋） cells after chemotherapy. Early removal of N^＋cells can block the malignant progress of tumor.2 However, few studies compared the PI between N^＋cells and glial fibrillary acidic protein （GFAP）-positive （GFAP^＋） cells in human astrocytic tumors. In the present study, we investigated the distribution and proliferation ofN^＋cells and GFAP^＋ cells in human astrocytic tumors to clarify the role these cells play in the cytopathology of these tumors.

Role of p38 Signaling Pathway in Pentagastrin-Regulated Cell Proliferation of Colorectal Carcinoma Cell Line HT-29

Objective: To investigate the effects and mechanisms of p38 signaling pathway in pentagastrin-regulated cell proliferation of colorectal carcinoma cell line HT-29. Methods: HT-29 cell line of colorectal carcinoma was in vitro incubated and divided into the control group, pentagastrin group, proglumide group, and pentagastrin + proglumide group. MTT reduction assay was performed to detect the proliferation status of HT-29 cell line and determine the optimal dosage of pentagastrin and proglumide. Annexin V-fluorescein isothiocyanate flow cytometry was used to detect the proliferation index (PI) and apoptosis rate (AR) of HT-29 cells. Reverse transcriptase polymerase chain reaction was performed to detect the mRNA expression of the pentagastrin receptor/cholecystokinin-B receptor (CCK-BR) and p38. The protein and phosphorylation levels of p38 were estimated by western blotting. Results: RT-PCR detection showed that CCK-BR mRNA was expressed in the HT-29 cell line. Pentagatrin improved HT-29 cell proliferation in dosage of 6.25 - 100 mg/L, and the optimal dosage of pentagastrin was 25.0 mg/L. Proglumide had no significant effect on the proliferation of HT-29 cells, but significantly inhibited the proliferation of HT-29 cells stimulated by pentagastrin when the dosage of proglumide was 8.0 - 128.0 mg/L, and the optimal dosage was 32.0 mg/L. The AR in the pentagastrin group was significantly lower than that in the control group and in the pentagastrin + proglumide group. The PI in the pentagastrin group was significantly higher than that in the control group and in the pentagastrin + proglumide group. P38 phosphorylation level in the pentagastrin group was significantly lower than that in the control group, and in the pentagastrin + proglumide group. There were no significant differences in the mRNA and protein expression of p38 in the control, pentagastrin, proglumide and pentagastrin + proglumide groups. Conclusion: Pentagastrin can improve proliferation of the CRC cell line HT-29 and inhibit apoptosis via the p38 signal transduction pathway. This mechanism may be associated with suppressed p38 protein phosphorylation level due to inhibition of proglumide, a gastrin receptor antagonist.

COMPARATIVE PATHOLOGICAL AND FCM STUDIES ON THE PRIMARY AND METASTATIC CANCERS IN STOMACH AND BREAST

Pathological morphology,differentiation,cellular DNA content and cell Proliferation index(PI) were comparatively studied in the primary and their corresponding metastatic lesions of 54 cases of gastric and breast cancers- The results showed that differentiation and types of metastatic cancers were in accordance with their corresponding primary cancers in more than half cases(18/13) of stomach cancers,differences could be found in the other cases(16/34),and among them,10 were lower and 6 were higher differentially than their corresponding primary cancers.Similar results were found in breast cancer cases.Flow cytometry(FCM) analysis revealed that dramatic DNA Index(DI) differences between metastatic and their corresponding primary cancers existed in 6/34 of gastric and 4/18 of mammary cancers,among them 7 cases were higher than the primary while 3 were lower than the primary(DI).Similar results could also be found in PI analysis.All these suggested that metastasis of gastric and breast cancer was a very complicated process and metastatic cancer did not necessarily always show lower differentiation,higher DNA contents and DNA aneuploidy as well as higher cell proliferative rate.

Research on liver regeneration driven by the amniotic membrane

Primary liver cancer is one of the most common cancers in China.Among all liver cancers,more than 90% of the cases are hepatocellular carcinoma （HCC） with a mortality of 20.40/0.1 million people.In general,surgery is the first-line treatment for primary liver cancer.With the development of diagnostic procedures,surgical technologies,and perioperative treatments,the mortality and complications of HCC have decreased.However,compared to other surgeries,the postoperative complications of hepatectomy have remained at relatively high levels.Of these complications,liver failure is one of the most important and common complications,and may lead to death.Functional protection after hepatectomy is crucial for the postoperative treatment and rehabilitation of patients.Derived from the cytotrophoblast,the amniotic membrane （AM） is the inner membrane of the fetal membranes.Keywords：amniotic membrane; liver regeneration; partial hepatectomy; proliferation index