目的探讨应用D-半乳糖(D-gal)建立小鼠肝脏损伤模型的方法.方法将20只小鼠随机分为两组,正常对照组应用生理盐水20 m L/(κg·d-1)皮下注射,模型组应用D-半乳糖按500 mg/(κg·d-1)皮下注射.造模完成后采用比色法测定血清AST,AL...目的探讨应用D-半乳糖(D-gal)建立小鼠肝脏损伤模型的方法.方法将20只小鼠随机分为两组,正常对照组应用生理盐水20 m L/(κg·d-1)皮下注射,模型组应用D-半乳糖按500 mg/(κg·d-1)皮下注射.造模完成后采用比色法测定血清AST,ALT及GST;放免法测定血浆PGE2,TNF及IL-6;Western blot方法检测肝组织COX-2表达.通过光镜观察肝组织病理变化.结果血浆ALT,AST及GST模型组与正常对照组比较有明显升高(P<0.01);模型组与正常对照组比较血浆PGE2,TNF及IL-6升高明显,差异具有统计学意义(P<0.01);病理切片显示肝脏结构呈现病理改变.结论应用D-半乳糖可以成功制作小鼠肝脏损伤模型.展开更多
Purpose We aimed to analyse the correlation between prostaglandin D2 synthase(PTGDS)and a diffuse large B-cell lymphoma(DLBCL)prognosis.Methods We retrospectively collected two hundred paraffin-embedded tissue specime...Purpose We aimed to analyse the correlation between prostaglandin D2 synthase(PTGDS)and a diffuse large B-cell lymphoma(DLBCL)prognosis.Methods We retrospectively collected two hundred paraffin-embedded tissue specimens that were pathologically diagnosed as DLBCL in the Fujian Tumour Hospital between January 2014 and December 2018.An abundance of paraffin-embedded tumour tissues were obtained.Twenty patients with lymphocyte-rich,benign,tissue-reactive,hypertrophic tonsillitis were selected as controls.Wax blocks were selected for primary cases and the controls were screened by professional pathologists.The levels of prostaglandin D2 synthase(PTGDS)and the EMT-related molecules,E-cadherin and vimentin,were detected by immunohistochemistry in clinical samples.A chi-square test revealed the correlations between PTGDS expression and clinicopathological characteristics,including age,sex,primary site,clinical stage,immunotyping,and International Prognostic Index(IPI)score.The Kaplan–Meier survival analysis was performed,and the diagnostic value was evaluated by receiver operating characteristic(ROC)curve analysis.Results A total of 138 cases(69%)were found to be PTGDS positive(>30%positive cells).PTGDS staining was negative(<30%positive cells)in 62 cases(31%).We collected the corresponding clinicopathological information and found that PTGDS expression was not significantly related to the patients’age,tumour stage,presence of extranodal invasion,or IPI score.According to the follow-up data,patients with low PTGDS expression had poor progression-free survival(PFS)and overall survival(OS),with 2-year PFS and OS rates of 41.7%and 50%,respectively.The 2-year PFS and OS rates of PTGDS-positive patients were 89.3%and 92.9%,respectively(P<0.0001),and the differences were significant.Conclusion We found that the expression level of PTGDS is significantly correlated with the prognosis of diffuse large B-cell lymphoma.展开更多
Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascul...Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascular diseases. This study was to research its effects on bone marrow-derived mast cell （BMMC）-mediated allergy and inflammation mechanisms. Methods： In this study, the biological effect of Cheongseoikki-tang ethanol extract （CITE） was evaluated, focusing on its effects on the production of allergic mediators by phorbol 12-myfistate 13-acetate （PMA） plus calcium ionophore A23187 （A23187）-stimulated BMMCs. These allergic mediators included intedeukin-6 （IL-6）, prostaglandin D2 （PGD2）, leukotfiene （34 （LTC4）, and β-hexosaminidase （13-hex）. Results： Our data revealed that CITE inhibited the production of IL-6, PGD2,/TC4, and 15-hex induced by PMA plus A23187 （P〈0.05）. Conclusion： These findings indicate that CITE has the potential for use in the treatment of allergy.展开更多
文摘目的探讨应用D-半乳糖(D-gal)建立小鼠肝脏损伤模型的方法.方法将20只小鼠随机分为两组,正常对照组应用生理盐水20 m L/(κg·d-1)皮下注射,模型组应用D-半乳糖按500 mg/(κg·d-1)皮下注射.造模完成后采用比色法测定血清AST,ALT及GST;放免法测定血浆PGE2,TNF及IL-6;Western blot方法检测肝组织COX-2表达.通过光镜观察肝组织病理变化.结果血浆ALT,AST及GST模型组与正常对照组比较有明显升高(P<0.01);模型组与正常对照组比较血浆PGE2,TNF及IL-6升高明显,差异具有统计学意义(P<0.01);病理切片显示肝脏结构呈现病理改变.结论应用D-半乳糖可以成功制作小鼠肝脏损伤模型.
基金supported by the Natural Science Foundation of Fujian Province(no.2020J011119)supported by the Natural Science Foundation of Fujian Province(no.2022J011046).
文摘Purpose We aimed to analyse the correlation between prostaglandin D2 synthase(PTGDS)and a diffuse large B-cell lymphoma(DLBCL)prognosis.Methods We retrospectively collected two hundred paraffin-embedded tissue specimens that were pathologically diagnosed as DLBCL in the Fujian Tumour Hospital between January 2014 and December 2018.An abundance of paraffin-embedded tumour tissues were obtained.Twenty patients with lymphocyte-rich,benign,tissue-reactive,hypertrophic tonsillitis were selected as controls.Wax blocks were selected for primary cases and the controls were screened by professional pathologists.The levels of prostaglandin D2 synthase(PTGDS)and the EMT-related molecules,E-cadherin and vimentin,were detected by immunohistochemistry in clinical samples.A chi-square test revealed the correlations between PTGDS expression and clinicopathological characteristics,including age,sex,primary site,clinical stage,immunotyping,and International Prognostic Index(IPI)score.The Kaplan–Meier survival analysis was performed,and the diagnostic value was evaluated by receiver operating characteristic(ROC)curve analysis.Results A total of 138 cases(69%)were found to be PTGDS positive(>30%positive cells).PTGDS staining was negative(<30%positive cells)in 62 cases(31%).We collected the corresponding clinicopathological information and found that PTGDS expression was not significantly related to the patients’age,tumour stage,presence of extranodal invasion,or IPI score.According to the follow-up data,patients with low PTGDS expression had poor progression-free survival(PFS)and overall survival(OS),with 2-year PFS and OS rates of 41.7%and 50%,respectively.The 2-year PFS and OS rates of PTGDS-positive patients were 89.3%and 92.9%,respectively(P<0.0001),and the differences were significant.Conclusion We found that the expression level of PTGDS is significantly correlated with the prognosis of diffuse large B-cell lymphoma.
基金Supported by the National Research Foundation of Korea Grant (No.NRF-2010-013-E00034)the Grant of the Traditional Korean Medicine R&D project,Ministry of Health & Welfare, Republic of Korea(No.B120069)
文摘Objective： Cheongseoikki-tang （CIT, Korean）, also called Qingshu Yiqi decoction（清署益气汤）and Seisho-ekki-to （Japanese）, is well known as an effective traditional combination of herbs for treating cardiovascular diseases. This study was to research its effects on bone marrow-derived mast cell （BMMC）-mediated allergy and inflammation mechanisms. Methods： In this study, the biological effect of Cheongseoikki-tang ethanol extract （CITE） was evaluated, focusing on its effects on the production of allergic mediators by phorbol 12-myfistate 13-acetate （PMA） plus calcium ionophore A23187 （A23187）-stimulated BMMCs. These allergic mediators included intedeukin-6 （IL-6）, prostaglandin D2 （PGD2）, leukotfiene （34 （LTC4）, and β-hexosaminidase （13-hex）. Results： Our data revealed that CITE inhibited the production of IL-6, PGD2,/TC4, and 15-hex induced by PMA plus A23187 （P〈0.05）. Conclusion： These findings indicate that CITE has the potential for use in the treatment of allergy.
