Objective:To investigate potential mechanism of Prostate prescriptionⅠin the treatment of chronic prostatitis based on integrated pharmacology.Methods:with the help of TCMIP,the effective compounds of Prostate prescr...Objective:To investigate potential mechanism of Prostate prescriptionⅠin the treatment of chronic prostatitis based on integrated pharmacology.Methods:with the help of TCMIP,the effective compounds of Prostate prescriptionⅠwere screened,and the potential target database of Prostate prescriptionⅠwas established by using the target prediction function of TCMIP.Based on the Human Phenotype Ontology database and protein interaction network(PPI)database,the disease target of CP was identified and the target interaction network was constructed;Through the GO function analysis and KEGG pathway enrichment analysis of the candidate targets,combined with Chinese and foreign literature studies to predict the potential Signaling pathway of Prostate prescriptionⅠin the treatment of CP.Results:79 effective compounds of Prostate prescriptionⅠexerted its effect on 33 targets through 11 pathways.Conclusion:Clinical efficacy of Prostate prescriptionⅠon CP may be reflected in antiinflammation and anti-tumor,cell metabolism,immune regulation,neuroregulation and so on.展开更多
Prostate cancer （PCa） is the most common visceral malignancy in men with androgen deprivation therapy （ADT） the preferred therapy to suppress testosterone production and hence tumor growth. Despite its effectivene...Prostate cancer （PCa） is the most common visceral malignancy in men with androgen deprivation therapy （ADT） the preferred therapy to suppress testosterone production and hence tumor growth. Despite its effectiveness in lowering testosterone, ADT is associated with side effects including loss of muscle mass, diminished muscle strength, decrements in physical performance, earlier fatigue and declining quality of life. This review reports a survey of the literature with a focus on changes in muscle strength, physical function and body composition, due to short-term and long-term ADT. Studies in these areas are sparse, especially well-controlled, prospective randomized trials. Cross-sectional and longitudinal data （up to 2 years） for men with PCa treated with ADT as well as patients with PCa not receiving ADT and age-matched healthy men are presented when available. Based on limited longitudinal data, the adverse effects of ADT on muscle function, physical performance and body composition occur shortly after the onset of ADT and tend to persist and worsen over time. Exercise training is a safe and effective intervention for mitigating these changes and initial guidelines for exercise program design for men with PCa have been published by the American College of Sports Medicine. Disparities in study duration, types of studies and other patient-specific variables such as time since diagnosis, cancer stage and comorbidities may all affect an understanding of the influence of ADT on health, physical performance and mortality.展开更多
目的:研究重楼皂苷Ⅰ(PPⅠ)对去势抵抗性人前列腺癌PC3细胞生长的抑制作用及其分子机制。方法:体外培养PC3细胞,予不同浓度(0.4、0.8、1.2、1.6、2.0、2.4μmol/L)的PPⅠ处理24、48、72 h,另设对照组,MTT法观察PPⅠ对PC3细胞增殖的影响...目的:研究重楼皂苷Ⅰ(PPⅠ)对去势抵抗性人前列腺癌PC3细胞生长的抑制作用及其分子机制。方法:体外培养PC3细胞,予不同浓度(0.4、0.8、1.2、1.6、2.0、2.4μmol/L)的PPⅠ处理24、48、72 h,另设对照组,MTT法观察PPⅠ对PC3细胞增殖的影响;流式细胞术检测细胞凋亡率;Western印迹检测PPⅠ对磷酸化细胞外调节蛋白激酶1/2(p-ERK1/2)、细胞外调节蛋白激酶1/2(ERK1/2)、核转录因子kappa B(NF-κB)/p65以及DNA甲基转移酶1(DNMT1)蛋白表达的影响,并加入ERK1/2抑制剂(PD98059)后检测PPⅠ对NF-κB/p65表达的影响。结果:MTT结果显示,PPⅠ能抑制PC3细胞的体外增殖,与对照组相比,PC3细胞从给药浓度0.4μmol/L(0.85±0.05 vs 1.00±0.00,P<0.01)开始明显下降,且呈时间和剂量依赖性。流式细胞术检测显示,PPⅠ能明显诱导PC3细胞早期凋亡,与对照组相比,PC3细胞早期凋亡率从给药浓度0.8μmol/L(13.83±2.97 vs 4.83±0.95)开始明显增加(P均<0.01),且呈剂量依赖性;Western印迹显示,PPⅠ以时间依赖性上调p-ERK1/2和ERK1/2蛋白的激活与表达,与对照组相比,给药时间从2 h(1.73±0.17 vs 1.00±0.00,P<0.01)开始,p-ERK1/2明显被激活表达,PPⅠ以剂量依赖性下调NF-κB/p65、DNMT1蛋白的表达,与对照组相比,给药浓度分别从1.6μmol/L(0.67±0.11 vs 1.00,P<0.01)与1.2μmol/L(0.63±0.06 vs 1.00±0.00,P<0.01)开始,NF-κB/p65、DNMT1表达明显下调;抑制ERK1/2磷酸化能逆转重楼皂苷Ⅰ对NF-κB/p65蛋白表达的下调,与PPⅠ组相比,PD98059+PPⅠ组NF-κB/p65蛋白表达(0.86±0.18 vs 0.43±0.09,P<0.05)明显上调。结论:PPⅠ可能通过介导ERK1/2通路抑制NF-κB/p65和DNMT1蛋白表达,诱导PC3细胞早期凋亡,进而抑制细胞增殖。展开更多
基金Natural Science Foundation of Jiangsu Province(No.BK20191498)Major School-level Project of Jiangsu Vocational College of Health and Health(No.JKA201903)。
文摘Objective:To investigate potential mechanism of Prostate prescriptionⅠin the treatment of chronic prostatitis based on integrated pharmacology.Methods:with the help of TCMIP,the effective compounds of Prostate prescriptionⅠwere screened,and the potential target database of Prostate prescriptionⅠwas established by using the target prediction function of TCMIP.Based on the Human Phenotype Ontology database and protein interaction network(PPI)database,the disease target of CP was identified and the target interaction network was constructed;Through the GO function analysis and KEGG pathway enrichment analysis of the candidate targets,combined with Chinese and foreign literature studies to predict the potential Signaling pathway of Prostate prescriptionⅠin the treatment of CP.Results:79 effective compounds of Prostate prescriptionⅠexerted its effect on 33 targets through 11 pathways.Conclusion:Clinical efficacy of Prostate prescriptionⅠon CP may be reflected in antiinflammation and anti-tumor,cell metabolism,immune regulation,neuroregulation and so on.
文摘Prostate cancer （PCa） is the most common visceral malignancy in men with androgen deprivation therapy （ADT） the preferred therapy to suppress testosterone production and hence tumor growth. Despite its effectiveness in lowering testosterone, ADT is associated with side effects including loss of muscle mass, diminished muscle strength, decrements in physical performance, earlier fatigue and declining quality of life. This review reports a survey of the literature with a focus on changes in muscle strength, physical function and body composition, due to short-term and long-term ADT. Studies in these areas are sparse, especially well-controlled, prospective randomized trials. Cross-sectional and longitudinal data （up to 2 years） for men with PCa treated with ADT as well as patients with PCa not receiving ADT and age-matched healthy men are presented when available. Based on limited longitudinal data, the adverse effects of ADT on muscle function, physical performance and body composition occur shortly after the onset of ADT and tend to persist and worsen over time. Exercise training is a safe and effective intervention for mitigating these changes and initial guidelines for exercise program design for men with PCa have been published by the American College of Sports Medicine. Disparities in study duration, types of studies and other patient-specific variables such as time since diagnosis, cancer stage and comorbidities may all affect an understanding of the influence of ADT on health, physical performance and mortality.
文摘目的:研究重楼皂苷Ⅰ(PPⅠ)对去势抵抗性人前列腺癌PC3细胞生长的抑制作用及其分子机制。方法:体外培养PC3细胞,予不同浓度(0.4、0.8、1.2、1.6、2.0、2.4μmol/L)的PPⅠ处理24、48、72 h,另设对照组,MTT法观察PPⅠ对PC3细胞增殖的影响;流式细胞术检测细胞凋亡率;Western印迹检测PPⅠ对磷酸化细胞外调节蛋白激酶1/2(p-ERK1/2)、细胞外调节蛋白激酶1/2(ERK1/2)、核转录因子kappa B(NF-κB)/p65以及DNA甲基转移酶1(DNMT1)蛋白表达的影响,并加入ERK1/2抑制剂(PD98059)后检测PPⅠ对NF-κB/p65表达的影响。结果:MTT结果显示,PPⅠ能抑制PC3细胞的体外增殖,与对照组相比,PC3细胞从给药浓度0.4μmol/L(0.85±0.05 vs 1.00±0.00,P<0.01)开始明显下降,且呈时间和剂量依赖性。流式细胞术检测显示,PPⅠ能明显诱导PC3细胞早期凋亡,与对照组相比,PC3细胞早期凋亡率从给药浓度0.8μmol/L(13.83±2.97 vs 4.83±0.95)开始明显增加(P均<0.01),且呈剂量依赖性;Western印迹显示,PPⅠ以时间依赖性上调p-ERK1/2和ERK1/2蛋白的激活与表达,与对照组相比,给药时间从2 h(1.73±0.17 vs 1.00±0.00,P<0.01)开始,p-ERK1/2明显被激活表达,PPⅠ以剂量依赖性下调NF-κB/p65、DNMT1蛋白的表达,与对照组相比,给药浓度分别从1.6μmol/L(0.67±0.11 vs 1.00,P<0.01)与1.2μmol/L(0.63±0.06 vs 1.00±0.00,P<0.01)开始,NF-κB/p65、DNMT1表达明显下调;抑制ERK1/2磷酸化能逆转重楼皂苷Ⅰ对NF-κB/p65蛋白表达的下调,与PPⅠ组相比,PD98059+PPⅠ组NF-κB/p65蛋白表达(0.86±0.18 vs 0.43±0.09,P<0.05)明显上调。结论:PPⅠ可能通过介导ERK1/2通路抑制NF-κB/p65和DNMT1蛋白表达,诱导PC3细胞早期凋亡,进而抑制细胞增殖。
