Effects of Slow Release Fertilizer on the Yield and Nutrient Use Efficiency of Carnation and Its Environmental Protection Effect

[Objective] The aim was to study the effects of slow release fertilizer on the yield,economic benefit and nutrient use efficiency of carnation and environmental pollution.[Method] Taking carnation（Dianthus caryophyllus）as research object,the application effect and environmental protection effect of slow release fertilizer on carnation were discussed through field plot test.[Result] The main agronomic characters of carnation improved after the application of slow release fertilizer;compared with Conv-F treatment,the yield of carnation with slow release fertilizer increased by 18.67%-20.83%,and its economic benefit increased by 105 500 yuan/hm2,while the ratio of output to input improved by 74.29%;under the same NPK ratio and nutrient amount,the yield,economic benefit and ratio of output to input of carnation after the application of slow release fertilizer increased by 2.11%,14 800 yuan and 16.2%,respectively;besides,the application of slow release fertilizer improved the nutrient use efficiency of carnation,and N,P and K nutrient use efficiency in Opt-F-0.7% treatment increased by 13.88%,8.57% and 30.14% compared with Conv-F treatment.[Conclusion] Slow release fertilizer could not only reduce the waste of fertilizer resources and improve fertilizer use efficiency but also decrease the pollution caused by nutrient loss,which had important practical significance for protecting ecological environment and promoting the sustainable development of agriculture.

Protective effects of baffles with different positions,row spacings,heights on debris flow impact

The baffle effectively slowed down debris flow velocity,reduced its kinetic energy,and significantly shortened the distance of debris flow movement.Consequently,they are widely used for protection against natural hazards such as landslides and mudslides.This study,based on the threedimensional DEM(Discrete Element Method),investigated the impact of different baffle positions on debris flow protection.Debris flow velocity and kinetic energy variations were studied through single-factor experiments.Suitable baffle positions were preliminarily selected by analyzing the influence of the first-row baffle position on the impact force and accumulation mass of debris flow.Subsequently,based on the selected baffle positions and four factors influencing the effectiveness of baffle protection(baffle position(P),baffle height(h),row spacing(S_(r)),and angle of transit area(α)),an orthogonal design was employed to further explore the optimal arrangement of baffles.The research results indicate that the use of a baffle structure could effectively slow down the motion velocity of debris flows and dissipate their energy.When the baffle is placed in the transit area,the impact force on the first-row baffle is greater than that when the baffle is placed in the deposition area.Similarly,when the baffle is placed in the transit area,the obstruction effect on debris flow mass is also greater than that when the baffle is placed in the deposition area.Through orthogonal experimental range analysis,when the impact on the first row of baffles is used as the evaluation criterion,the importance of each influencing factor is ranked asα>P>S_(r)>h.When the mass of debris flow behind the baffle is regarded as the evaluation criterion,the rank is changed to P>α>S_(r)>h.The experimental simulation results show that the optimal baffle arrangement is:P_(5),S_(r)=16,α=35°,h=9.

Study on the effect of four kinds of raw materials in hypertonic dehydration cell model

It aims to investigate the protective effects of sodium hyaluronate,panthenol,Portulaca oleracea L.and Calendula officinalis L.on hyperosmotic dehydration-induced injury of human immortalized keratinocytes(HaCaT).The safety mass concentrations of four raw materials were screened by detecting cell viability,and the secretion of hyaluronic acid(HA)was determined using the ELISA method.The expression of HaCaT barrier function related genes(OVOL1,EREG,TGM1,TGM2,IVL,IRF6,THBS1,CASP14)was detected at the mRNA level to explore the regulatory effect of four raw materials on these genes.The results demonstrate that pretreatment with the four kinds of raw materials could increase the cell viability after hyperosmotic dehydration,promote the secretion of HA,and improve the expression of barrier function related genes after hyperosmotic dehydration,among which panthenol and Calendula officinalis L.are better.The results show that the four raw materials have a certain protective effect on the hyperosmotic dehydration cell model,which provides data support for its application in cosmetics.

Acute Toxicity of Jinchuan Formula Plum Wine Extract and Its Protective Effect on Mice with Liver Injury

[Objectives]To investigate the acute toxicity and hepatoprotective effect of Jinchuan formula plum wine extract on mice,determine its safety range,and evaluate its hepatoprotective effect.[Methods]The median lethal dose(LD_(50))was determined by acute toxicity test with the toxic reaction and mortality of mice as indexes.Sixty Kunming mice were randomly divided into 6 groups:normal control group,model group(ConA-induced liver injury model),Jinchuan formula plum wine high,medium and low dose groups(1.0,0.5,0.25 g/kg)and silybin group(0.1 g/kg).The levels of ALT,AST,LDH in serum and TG,VLDL in liver were measured.After HE staining,the pathological changes of liver tissue in mice were observed,and the liver protective effect of Jinchuan formula plum wine extract was analyzed and evaluated.[Results]LD_(50)was 11.18 g/kg,and the 95%confidence limit of LD_(50)was 10.31-12.05 g/kg.The high-dose group of Jinchuan formula plum wine extract could significantly reduce the serum ALT and AST activities of ConA-induced liver injury mice(P<0.05).[Conclusions]Jinchuan formula plum wine extract is relatively safe,and also has a protective effect on liver injury.

Protective effect of Solanum Nigrum Linn green fruit ethanolic extract on alcoholic liver injury in mice

Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,promoting blood and detumescence.Solanum Nigrum Linn fruit contains a variety of antioxidant enzymes,can remove the body produced by aerobic metabolism harmful substances.In this paper,a model of alcohol-induced liver injury in C57BL/6 mice was established to evaluate the protective effect of Solanum Nigrum Linn green fruit(SNGF)ethanolic extract on alcohol-induced liver injury.H&E staining and oil red O(ORO)staining showed that hepatic lobules were clearly demarcated,vacuoles were significantly reduced and lipid droplets were reduced in SNGF ethanolic extract treatment group.Serum levels of TC,TG,LDH,TBA,AKP,ALT and AST were decreased in the SNGF ethanolic extract treatment group,and SNGF ethanolic extract could clear reactive oxygen species(ROS)in time.MDA content was signifi cantly decreased after SNGF ethanolic extract treatment,while superoxide dismutase(SOD)and GSH-Px contents were increased after SNGF ethanolic extract treatment.These results suggest that SNGF ethanolic extract has a protective effect on alcohol-induced liver injury.

Ecosystem assessment and protection effectiveness of a tropical rainforest region in Hainan Island, China

Ecosystem services have become one of the core elements of ecosystem management and evaluation. As a key area of ecosystem services and for maintaining national ecological security, ecosystem changes and implementation effect evaluation are important in national key ecological function zones, for promoting the main function zone strategy and for improving the construction of an ecological civilization. This article studies the ecological zone of a tropical rainforest region in the central mountain area of Hainan Island, China. Multi-source satellite data and ground observation statistics are analyzed with geo-statistics method and ecological assessment model. The core analysis of this paper includes ecosystem patterns, quality and services. By means of spatial and temporal scale expansion and multidimensional space-time correlation analysis, the trends and stability characteristics of ecosystem changes are analyzed, and implementation effect evaluation is discussed. The analysis shows a variety of results. The proportion of forest area inside the ecological zone was significantly higher than the average level in Hainan Island. During 1990–2013, settlement gradually increased inside the ecological zone. After implementation of the zone in 2010, human activity intensity increased, with the main land use being urban construction and land reclamation. Water conservation in the ecological function zone was higher than that outside the zone. In general, it increased slightly, but had obvious fluctuations. Soil conservation inside the zone was also better than that outside. However, it demonstrated dramatic fluctuations and relatively poor stability during 1990–2013. The human disturbance index inside the zone was significantly lower than that outside, and had a lower biodiversity threat level. Especially in 2010–2013, the increased range of the human disturbance index inside the zone was significantly less than that outside.

A facile and sensitive colorimetric detection for RNase A activity based on target regulated protection effect on plasmonic gold nanoparticles aggregation

In this work,a facile and sensitive colorimetric detection method was firstly reported for RNase A activity detection based on target regulated protection effect of chimeric DNA probe on the salt-induced aggregation of plasmonic gold nanoparticles.Compared with previous works of RNase A activity detection,this colorimetric assay integrated the advantages of sensitive,low cost,facile operation,rapid response and low biological toxicity.

Mechanical analysis of effective pressure relief protection range of upper protective seam mining

This paper analyzes the control mechanism of coal and gas outbursts and proposes the concept of an effective pressure relief protection range, based on the stress relief of the underlying coal-rock mass and the development of a plastic zone. Also this study developed a stress change and fracture development model of the underlying coal-rock mass. In addition, the stress and depth of fracture of any point in the floor were deduced with the application of Maple Calculation Software. The specific engineering parameters of the Pingdingshan No. 12 colliery were applied to determine the relationship between the depth of fracture in the floor and the mining height. The pressure-relief principle of the underlying coal-rock mass was analyzed while varying the mining height of the upper protective seam. The findings indicate that as the depth of fracture in the floor increases, the underlying coal-rock mass experiences a limited amount of pressure relief, and the pressure relief protection range becomes narrower.Additionally, the stress distribution evolves from a ‘‘U" shape into a ‘‘V" shape. A 2.0 m mining height of protective seam situates the outburst-prone seam, Ji_(15), within the effective pressure relief protection range. The fracture development and stress-relief ratio rises to 88%, ensuring the pressure-relief effect as well as economic benefits. The measurement data show that: after mining the upper protective seam, the gas pressure of Ji_(15) dropped from 1.78 to 0.35 MPa, demonstrating agreement between the engineering application and the theoretical calculation.

Protective effects of peptide KSPLY derived from Hericium erinaceus on H_(2)O_(2)-induced oxidative damage in HepG2 cells

Reactive oxygen species(ROS)-induced oxidative damage is strongly associated with the pathogenesis of chronic diseases,and natural antioxidant peptides have good abilities of scavenging ROS.The antioxidant activity of peptide Lys-Ser-Pro-Leu-Tyr(KSPLY)derived from Hericium erinaceus remains unclear.In the present study,the antioxidant effect and mechanism of KSPLY on H_(2)O_(2)-induced oxidative damage in HepG2 cells were investigated.The results indicated that KSPLY exhibited the antioxidant capacity in H_(2)O_(2)-induced HepG2 cells by enhancing superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and catalase(CAT)activities.In comparison with the H_(2)O_(2)-treated damage group,the apoptosis rate,ROS level,and malondialdehyde(MDA)content of HepG2 cells treated with KSPLY were significantly decreased.The H.erinaceus-derived peptide KSPLY pretreatment promoted the expression of detoxification and antioxidant enzymes via the Keap1/Nrf2 signal pathway,thereby inhibiting the generation of ROS and MDA.In conclusion,the H.erinaceus-derived peptide KSPLY effectively protected HepG2 cells against H_(2)O_(2)-induced oxidative damage,and it provided a theoretical basis for the further development of new natural antioxidants.

Protective Effect of Ethanol Extract from Sweet Potato Leaves on CCL_(4)-Induced Liver Injury in Mice

[Objectives]To investigate the protective effect of ethanol extract from sweet potato leaves on liver injury induced by CCl_(4)in mice.[Methods]25 ICR mice were randomly divided into blank group,model group,high-dose extract group(200 mg/kg),low-dose extract group(100 mg/kg)and positive control group(2 mg/kg colchicine),with 5 mice in each group.All groups except the blank group were given intraperitoneal injection of 20%CCl 4 olive oil solution(2 mL/kg),and the blank group was given the same dose of olive oil solution three times a week.After 4 weeks,each administration group was given the corresponding dose of drugs(10 mL/kg),and the blank group and model group were given the corresponding amount of normal saline for 2 weeks.After the last intragastric administration,fasting was required,but water was allowed,blood was taken from eyeballs,and upper serum was taken by static centrifugation.Serum AST,ALT,CRP,IL-6 and SOD levels were detected by the kit.[Results]Compared with the blank group,the serum AST and ALT levels in the model group were significantly increased;compared with the model group,the ethanol extract of sweet potato leaves could decrease the levels of ALT,AST,CRP,IL-6 and increase the level of SOD in serum.[Conclusions]The ethanol extract of sweet potato leaves had protective effect on the mice with liver injury induced by CCl_(4),and its mechanism may be to protect the liver by lowering enzymes,inhibiting inflammation and antioxidant stress.

The Effect of Evidence-based Management on Occupational Protection of Nurses Who Perform Chemotherapy

Objective:The study was to explore the effect of evidence-based management in occupational protection for nurses who perform chemotherapy.Methods:40 chemotherapy nurses were selected,20 of them were included in the experimental group for evidence-based management,and the other 20 were included in the control group for routine management.Results:Compared with the control group,there were significantly fewer adverse nursing events in the experimental group,and P<0.05.Conclusion:The effect of evidence-based management in occupational protection of nurses who perform chemotherapy is significant.

欧盟推动“禁止强迫劳动产品”立法:规范冲突与现实挑战

欧盟推动“禁止强迫劳动产品”立法,将对国际经贸环境包括我国国际经贸活动产生深远影响。欧盟相关文件和立法提案,实质是“工商业与人权”规范的欧盟版,具有区域法治规范效果外溢化和人权规则工具化的双重意涵。在制定和实施上述法规时,欧盟应当信守国际法基本原则,尊重联合国相关倡议的宗旨和精神,履行WTO关于发达成员不实施贸易壁垒的义务。在实践层面,欧盟新法规将提高发展中国家进入欧盟市场和全球供应链的成本,恶化发展中国家的外贸和就业环境,最终损害发展中国家劳工利益。对此,可采取以下对策:加强与欧盟沟通,要求欧盟克制贸易保护主义倾向,避免将人权法规工具化;主动对标高标准国际经贸规则、促进深层次改革,持续扩大法治保障下的高水平开放;加强对企业的合规指导,引导企业增强国际竞争力;针对欧盟相关法规及其实施造成的潜在不利影响,做好务实管用的预案,以预防风险。

Mediated protective effect of electroacupuncture pretreatment by miR-214 on myocardial ischemia/reperfusion injury

Background Electroacupuncture pretreatment plays a protective role in myocardial ischemia/reperfusion （I/R） injury and microRNAs （miRNAs） could act on various facets of cardiac function. However, the role of miRNAs in the cardioprotection by electroacupuncture pre-treatment on myocardial I/R injury remains unknown. The purpose of the study was to examine whether miR-214 was involved in cardio-protection by electroacupuncture. Methods Using rat myocardial I/R model, we examined the role of electroacupuncture pretreatment in myocardial I/R injury and analyzed the changes in the expression of miR-214. In addition, I/R was simulated in vitro by performing oxy-gen-glucose deprivation （OGD） on H9c2 cell cultures, and the effect of electroacupuncture pretreatment on I/R injury as well as expressional level of miR-214 were examined in vitro. Furthermore, the miR-214 mimic was transfected into OGD-treated H9c2 cells, we analyzed the cell apoptosis, lactate dehydrogenase （LDH） and creatine kinase （CK） activities, intracellular free Ca2＋concentration （[Ca2＋]i） as well as the relative protein levels of sodium/calcium exchanger 1（NCX1）, BCL2-like 11 （BIM）, calmodulin-dependent protein kinase IIδ（CaMKIIδ） and Cyclophilin D （CypD）. Results The in vivo results revealed that compared with the I/R group, the electroacupuncture pretreatment group showed significant decreased myocardial infarct size, as well as the increased indices of the cardiac function, including heart rate, mean arterial pressure, left ventricular systolic pressure and maximal rate for left ventricular pressure rising and declining （±dp/dt max）. In addition, electroacupuncture pretreatment could inhibit the elevation of LDH and CK activities induced by I/R injury. The quantitative PCR （qPCR） results demonstrated electroacupuncture pretreatment could provide cardioprotection against myocardial I/R injury in rats with miR-214 up-regulation. In the meanwhile, in vitro, electroacupuncture pretreatment protected H9c2 cells from OGD-induced injury. Trans-fection of miR-214 mimic showed protective effects on OGD-induced injury to H9c2 cells by reducing apoptosis, decreasing LDH and CK activities, rescuing the OGD-induced Ca2＋and down-regulating elevated protein levels of NCX1, BIM, CaMKIIδand CypD. Conclusions Our findings firstly demonstrated that electroacupuncture pretreatment promotes the expression of miR-214 in myocardial I/R injury and miR-214 contributes to the protective effect of electroacupuncture on myocardial I/R injury.

Alleviation Effect of Lanthanum on Cadmium Stress in Seedling Hydroponic Culture of Kidney Bean and Corn

The seedling hydroponic culture experiment of kidney bean （ Phaseolus vulgaris ） and corn （ Zea mays ） was conducted to investigate the alleviation effect of lanthanum on Cd stress. It is found that growth is seriously inhibited and metabolism is maladjusted in the two crops under 30 and 300 μmol·L^-1 Cd^2＋ stress. Plant height, taproot length, leaf area, and fresh or dry weight of root, stem, and leaf are all obviously decreased. Further, chlorophyll content decreases, membrane permeability, malonydialdehyde （MDA） content, activities of catalase （CAT） and peroxidase （POD） increases under Cd stress, as compared with the control. The damage to these two crops becomes more conspicuous with the prolongation of Cd stress. It is suggested that lanthanum might help kidney bean and corn seedlings alleviate Cd stress by improving the photosynthetic capacity, reducing membrane permeability and MDA content, and maintaining the activities of CAT and POD of these two crops.

Protective effects of human umbilical cord mesenchymal stem cell vein transplantation against spinal cord ischemia/reperfusion injury in rats

BACKGROUND： The majority of studies addressing spinal cord ischemia/reperfusion injury （SCIRI） have focused on drugs, proteins, cytokines, and various surgical techniques. A recent study reports that human umbilical cord mesenchymal stem cell （hUCMSC） transplantation achieves good therapeutic effects, but the mechanisms underlying nerve protection remain poorly understood. OBJECTIVE： To observe survival of transplanted hUCMSCs in SCIRI rat models and the influence on motor function in the hind limbs, to determine interleukin-8 expression and cellular apoptosis in spinal cord tissues, and to verify the hypothesis that hUCMSC transplantation exhibits protective effects on SCIRI. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of the Department of Orthopedics in the First Affiliated Hospital of Soochow University, China between January 2007 and December 2008. MATERIALS： hUCMSCs were harvested from umbilical cord blood of healthy pregnant women after parturition in the Obstetrical Department of the First Affiliated Hospital of Soochow University, China. Rabbit anti-human BrdU monoclonal antibody was provided by DAKO, USA. Terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） Kit and enzyme-linked immunosorbent assay （ELISA） Kit were purchased by Wuhan Boster, China. METHODS： A total of 72 healthy, Wistar, adult rats were randomly assigned to three groups： sham-surgery, model, and transplantation, with 24 rats in each group. SCIRI was induced in the model and transplantation groups via the abdominal aorta block method. The infrarenal abdominal aorta was not blocked in the sham-surgery group. Prior to abdominal aorta occlusion, 0.2 03 mL bromodeoxyuridine （BrdU）-Iabeled hUCMSCs suspension （cell concentration 5 × 10 3/uL） was injected through the great saphenous vein of the hind limb, and an equal volume of physiological saline was administered to the model and sham-surgery groups. MAIN OUTCOME MEASURES： Pathological observation of rat spinal cord tissues was performed by hematoxylin-eosin staining at 6, 24, and 48 hours post-surgery. Immunohistochemistry was applied to determine hUCMSCs survival in the spinal cord. The amount of cellular apoptosis and interleukin-8 expression in spinal cord tissues was assayed utilizing the TUNEL and ELISA methods, respectively. Motor function in the hind limbs was evaluated according to Jacob＇s score. RESULTS： Numerous BrdU-positive cells were observed in spinal cord tissues from the transplantation group. The number of apoptotic cells and interleukin-8 levels significantly decreased in the transplantation group （P 〈 0.05）, pathological injury was significantly ameliorated, and motor function scores significantly increased （P 〈 0.05） compared with the model group. CONCLUSION： Via vein transplantation, hUCMSCs were shown to reach and survive in the injury area. Results suggested that the transplanted hUCMSCs contributed to significantly improved pathological changes in the injured spinal cord, as well as motor function, following SCIRI. The protective mechanism correlated with inhibition of cellular apoptosis and reduced production of inflammatory mediators.

Protective Effects of Ginsenoside Rb1 on Septic Rats and Its Mechanism

This study aims to observe the protective effects of ginsenoside Rbl on liver and lung in rats with septic shock and reveal its mechanism. Rats were randomly divided into three groups： sham, cecal ligation and puncture （CLP）, and CLP with ginsenoside Rb1. Then, the survival rate, arterial blood pressure, TLR4 mRNA, and TNF-α levels were determined. The liver and lung tissues were stained with hematoxylin-eosin （HE）. The overall survival rate of the Rb1 group was significantly higher than that of the CLP group. Mean arterial blood pressure went down in both the CLP and Rb1 groups after CLP, and there was a significant difference both in the sham and Rb1 groups when compared with the CLP group. The Rb1 treatment group had markedly lower TLR4 mRNA expression and TNF-a levels than the CLP group. In the CLP group, pathology showed swelling, degeneration, necrosis, and neutrophii infiltration in the liver and alveolar epithelial cells. However, in the Rb1 group, there was mild degeneration and slight neutrophil infiltration, but no obvious necrosis. Rb1 may improve the survival rate, ameliorate arterial blood pressure, and protect the liver and lung in septic shock rats by downregulating the expression of TLR4 mRNA and inhibiting the production of TNF-α.

Protective Effects of Shikonin on Brain Injury Induced by Carbon Ion Beam Irradiation in Mice

Radiation encephalopathy is the main complication of cranial radiotherapy. It can cause necrosis of brain tissue and cognitive dysfunction. Our previous work had proved that a natural antioxidant shikonin possessed protective effect on cerebral ischemic injury. Here we investigated the effects of shikonin on carbon ion beam induced radiation brain injury in mice. Pretreatment with shikonin significantly increased the SOD and CAT activities and the ratio of GSH/GSSG in mouse brain tissues compared with irradiated group （P〈0.01）, while obviously reduced the MDA and PCO contents and the RO$ levels derived from of the brain mitochondria.

Protective Effect of Dimethyl-4,4'-Dimethoxy-5,6,5',6'-Dimethylene Dioxybiphenyl-2,2'-Dicarboxylate (DDB) against Carcinogen-Induced Rat Liver Nuclear DNA Damage

The protective effect of DDB against carcinogen-induced DNA damage was examined in the present investigation. Preincubation of rat liver nuclei with DDB (1 mmol.L-1) resulted in 60% inhibition of binding of 3H-benzo (a) pyrene to nuclear DNA. Unscheduled DNA synthesis (UDS) induced by aflatoxin BI (10^(-7) mol.L-1) in freshly isolated rat hepatocytes was also inhibited by DDB (10^(-6)-10^(-3)mol.L-1). Oral administration of DDB at 200 mg.kg-1 once daily for 3 d induced a significant increase of liver cytosol glutathione-S-transferase and microsomal UDPG-transferase activity in mice. These results indicate that DDB is able to directly or indirectly antagonize certain carcinogen-induced DNA damages.

Evaluation of Ecological Effectiveness of Protected Areas in Northwest China

Protected areas(PAs) have experienced explosive growth in Northwest China over the last three decades, but their effectiveness in representing regional ecological system diversity has not attracted considerable attention. Low effectiveness would exacerbate the conservation-development conflicts, particularly those that arise as a result of the Great Western Development Strategy(GWDS). Thus, an assessment of the effectiveness of the PA network has become quite important. We proposed natural vegetation communities to represent regional ecological system diversities, and proposed Global 200 Priority Ecoregions, Important Bird Areas, and ecosystem function regions to represent important conservation areas. To determine their effectiveness, we studied the extent to which ecological system diversities and important conservation areas are represented by the existing 96 PAs. Our results indicated that the total coverage of vegetation communities in PAs in Northwest China is not sufficiently comprehensive. As the PA system has expanded, the growth in the total area of the PAs has been greater than that of their vegetation community richness. While most of the important conservation areas are covered by PAs, some regions have not yet reached the 10% threshold; further, PAs are distributed unevenly and conservation gaps remain in the region. Therefore, these regions should receive more attention when planning new PAs. It is vital that more biodiversity datasets and assessment of ecosystem function regions are integrated in order to provide a basis for the government to formulate appropriate protection and development strategies.

Protective Effect of Mulberry Extract against Pb-induced Learning and Memory Deficits in Mice

Lead （Pb） is ubiquitous in the environment, and low-level Pb exposure can cause neurotoxicity and irreversible damage to children＇s cognition, learning and memory ability. Nutritional intervention is an effective method to prevent Pb poisoning. Mul- berry is rich in anthocyanins, possessing protective effects for nerves. This study investigated the neuroprotective effects of mulberry extract （ME） against Pb-induced learning and memory deficits in mice. The results showed that the learning and memory abilities of mice, assessed using the Morris test, improved significantly after treatment with ME at a dose of 100 mg/kg body weight. The level of Pb in the brains of mice in the three ME intervention groups decreased significantly, while NO production and anti-oxidant enzymes were significantly restored. It is suggested that ME inhibits Pb-induced neurotoxicity by reversing Pb-induced alterations in the aspect of neurotoxic effects and improving learning and memory.