Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra

Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization（NPA-SH） probe that targets the large subunit of ribosomal RNA（LSU r RNA）. We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H.triquetra at a concentration of 1.5×10^4 cells/m L, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015（3.0×10^4 cells/m L）. We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H.triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.

Developing a power monitoring and protection system for the junction boxes of an experimental seafloor observatory network

A power monitoring and protection system based on an embedded processor was designed for the junction boxes(JBs) of an experimental seafloor observatory network in China. The system exhibits high reliability, fast response, and high real-time performance. A two-step power management method which uses metal-oxide-semiconductor field-effect transistors(MOSFETs) and a mechanical contactor in series was adopted to generate a reliable power switch, to limit surge currents and to facilitate automatic protection. Grounding fault diagnosis and environmental monitoring were conducted by designing a grounding fault detection circuit and by using selected sensors, respectively. The data collected from the JBs must be time-stamped for analysis and for correlation with other events and data. A highly precise system time, which is necessary for synchronizing the times within and across nodes, was generated through the IEEE 1588(precision clock synchronization protocol for networked measurement and control systems) time synchronization method. In this method, time packets were exchanged between the grandmaster clock at the shore station and the slave clock module of the system. All the sections were verified individually in the laboratory prior to a sea trial. Finally, a subsystem for power monitoring and protection was integrated into the complete node system, installed in a frame, and deployed in the South China Sea. Results of the laboratory and sea trial experiments demonstrated that the developed system was effective, stable, reliable, and suitable for continuous deep-sea operation.

Study on the Corrosion inhibitor and Fog Suppressor for Chemical Pickling of Iron and Steel

A new type of corrosion inhibitor and fog suppressor composed of Nitrogen-containing alkaloid,water-soluble butadiene lower polymer, and inorganic electrolyte has been investigated by gravimetric and electrochemical method. Effects or this chemicals on pickling rate and hydrogen penetration into iron and steel material in 50～150 g/L HCI or/and H2SO4 solutions at 20～70℃ temperature were examined. The amount of acid fog escaping from the surface of air-liquid was determined by chemical titration. The results indicate that the efficiency of inhibition and suppression depends on film properties by which mean a barrier film on the interface of bare mild steel/solution or an unsolvable liquid membrane as hydrophibic effect.In present work the film-forming mechanism by in situ and chemistry-mechanics effect is also discussed.

Smart Substation Integration Technology and Its Application in Distribution Power Grid

At present,smart substations use the IEC61850 standard based on the architectural framework of three layers and two networks to realize information digitization and advanced applications.Although the smart substation offers many improvements in design,equipment manufacturing,and construction,the intelligent devices used in smart substations are costly,and are also difficult to maintain since they are dispersed within a single unit.Functionality optimization and device integration,thus,have become important issues in smart substation development.This paper presents an integrated solution and implementation process for a smart substation system.In the process layer,an integrated intelligence component is developed that functions both as an intelligent terminal and a merging unit.In the bay layer,an integrated station-area protection measurement and control master device is designed to achieve such functions as protection,monitoring,control,fault recorder,and power quality monitoring.Finally,in the station control layer,an integrated information platform is established to bring together various system functions and to promote interactive sharing.Integration technology improves the economy and practicality of the smart substation,especially in a distribution power grid.