目的探讨H3.3G34W、p63及SATB2在骨巨细胞瘤(giant cell tumor of bone,GCTB)中的表达情况及其联合应用对GCTB的诊断作用和价值。方法收集西安交通大学附属红会医院病理科2020年至2022年诊断的54例GCTB、83例非骨巨细胞瘤(non-giant cel...目的探讨H3.3G34W、p63及SATB2在骨巨细胞瘤(giant cell tumor of bone,GCTB)中的表达情况及其联合应用对GCTB的诊断作用和价值。方法收集西安交通大学附属红会医院病理科2020年至2022年诊断的54例GCTB、83例非骨巨细胞瘤(non-giant cell tumor of bone,NGCTB)(包含14例动脉瘤样骨囊肿、16例软骨母细胞瘤和53例非骨化性纤维瘤)患者的样本和病历资料,采用免疫组织化学EliVision法检测H3.3G34W、p63及SATB2的表达情况。通过χ^(2)检验判断H3.3G34W、p63及SATB2的阳性率在各组间是否存在统计学差异;通过Logistic回归分析建立包括H3.3G34W、p63及SATB2的联合诊断模型,通过受试者工作特征(ROC)曲线分析评价模型的诊断价值。结果H3.3G34W、p63及SATB2在GCTB组中阳性率分别为81.5%、90.7%、92.6%;在NGCTB组中阳性率分别为2.4%、28.9%、62.7%。与NGCTB组相比,GCTB组患者年龄显著较大[(41.222±14.849)vs.(16.566±9.439);P<0.001],女性比男性患病率更高(51.9%vs.48.1%,P<0.001)。与NGCTB组相比,GCTB组中H3.3G34W(81.5%vs.2.4%,P<0.001);p63(90.7%vs.28.9%,P<0.001)和SATB2(92.6%vs.62.7%,P<0.001)的阳性率更高。单因素Logistic回归分析构建单因素预测模型,同时行ROC曲线分析,表明年龄(AUC=92.9%,P<0.001)、性别(AUC=64.5%,P=0.004)、H3.3G34W阳性率(AUC=89.5%,P<0.001)、p63阳性率(AUC=80.9%,P<0.001)、SATB2阳性率(AUC=65.0%,P=0.003)是GCTB诊断的独立预测因素。进一步的多因素Logistic回归分析构建混合预测模型,并行ROC曲线分析,发现混合模型展现出比单因素模型更好的预测价值(AUC=98.4%,P<0.001)。结论H3.3G34W、p63及SATB2是有效诊断GCTB的分子标记物,且三者联合应用更能提高GCTB的诊断预测效能。展开更多
Retinal ganglion cell apoptotic death is the main pathological characteristic of glaucoma,which is the leading cause of irreversible blindness.Disruption of Ca^(2+)homeostasis plays an important role in glaucoma.Volta...Retinal ganglion cell apoptotic death is the main pathological characteristic of glaucoma,which is the leading cause of irreversible blindness.Disruption of Ca^(2+)homeostasis plays an important role in glaucoma.Voltage-gated Ca^(2+)channel blockers have been shown to improve vision in patients with glaucoma.However,whether and how voltage-gated Ca^(2+)channels are involved in retinal ganglion cell apoptotic death are largely unknown.In this study,we found that total Ca^(2+)current densities in retinal ganglion cells were reduced in a rat model of chronic ocular hypertension experimental glaucoma,as determined by whole-cell patch-clamp electrophysiological recordings.Further analysis showed that L-type Ca^(2+)currents were downregulated while T-type Ca^(2+)currents were upregulated at the later stage of glaucoma.Western blot assay and immunofluorescence experiments confirmed that expression of the Ca_(V)1.2 subunit of L-type Ca^(2+)channels was reduced and expression of the Ca_(V)3.3 subunit of T-type Ca^(2+)channels was increased in retinas of the chronic ocular hypertension model.Soluble tumor necrosis factor-α,an important inflammatory factor,inhibited the L-type Ca^(2+)current of isolated retinal ganglion cells from control rats and enhanced the T-type Ca^(2+)current.These changes were blocked by the tumor necrosis factor-αinhibitor XPro1595,indicating that both types of Ca^(2+)currents may be mediated by soluble tumor necrosis factor-α.The intracellular mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and nuclear factor kappa-B signaling pathway mediate the effects of tumor necrosis factor-α.TUNEL assays revealed that mibefradil,a T-type calcium channel blocker,reduced the number of apoptotic retinal ganglion cells in the rat model of chronic ocular hypertension.These results suggest that T-type Ca^(2+)channels are involved in disrupted Ca^(2+)homeostasis and apoptosis of retinal ganglion cells in glaucoma,and application of T-type Ca^(2+)channel blockers,especially a specific CaV3.3 blocker,may be a potential strategy for the treatment of glaucoma.展开更多
本文以Halliday系统功能语言学中的及物性系统为理论框架,选取《经济学人》中ChatGPT系列报道为语料,运用及物性标注软件UAM Corpus Tool 3.3对新闻报道语料进行标注和成分分析,并探索新闻报道的语篇深层含义。本研究主要回答以下三个问...本文以Halliday系统功能语言学中的及物性系统为理论框架,选取《经济学人》中ChatGPT系列报道为语料,运用及物性标注软件UAM Corpus Tool 3.3对新闻报道语料进行标注和成分分析,并探索新闻报道的语篇深层含义。本研究主要回答以下三个问题:1) 在所选新闻语料中,及物性参与者分布是怎样的?2) 新闻语料中的及物性六大过程成分的分布如何?3) 新闻语料中的环境成分的分布如何?本文揭示了隐藏在西方国家新闻报道背后的态度,这将有助于启发读者理解新闻报道背后的态度及所隐含的意义。展开更多
通过扫描电镜/能谱、X射线衍射以及金相分析,针对含0.3%Fe(质量分数)的Al Mg Si Cu铝合金,研究了Mn含量对其结晶相的影响。研究表明:合金在铸造过程中形成的结晶相为Al1.9CuMg4.1Si3.3,Al5(FeMn)Si,Al8(FeMn)2Si以及少量的Mg2Si;增大含M...通过扫描电镜/能谱、X射线衍射以及金相分析,针对含0.3%Fe(质量分数)的Al Mg Si Cu铝合金,研究了Mn含量对其结晶相的影响。研究表明:合金在铸造过程中形成的结晶相为Al1.9CuMg4.1Si3.3,Al5(FeMn)Si,Al8(FeMn)2Si以及少量的Mg2Si;增大含Mn量,合金中AlFeMnSi型结晶相数量增多;对合金进行均匀化处理时,Al1.9CuMg4.1Si3.3相完全溶解,发生Al5(FeMn)Si向Al8(FeMn)2Si相的转变;对合金进行轧制及最终热处理后,结晶相碎化且沿轧向呈纤维状分布,但结晶相的类型不变。展开更多
传统的镁熔液第一气泡检测结果存在较大滞后性,且以Matlab为代表的检测工具又受限于工程应用,本文提出一种基于DSP检测弱小目标的新思路,构建了一个以DM642为主处理器的嵌入式图像处理系统平台,并利用C语言在DSP集成开发软件Code Compos...传统的镁熔液第一气泡检测结果存在较大滞后性,且以Matlab为代表的检测工具又受限于工程应用,本文提出一种基于DSP检测弱小目标的新思路,构建了一个以DM642为主处理器的嵌入式图像处理系统平台,并利用C语言在DSP集成开发软件Code Composer Studio 3.3上进行算法编程,实现了镁熔液第一气泡的检测。实验结果验证了镁熔液第一气泡检测方法在实际应用中的可行性。展开更多
文摘目的探讨H3.3G34W、p63及SATB2在骨巨细胞瘤(giant cell tumor of bone,GCTB)中的表达情况及其联合应用对GCTB的诊断作用和价值。方法收集西安交通大学附属红会医院病理科2020年至2022年诊断的54例GCTB、83例非骨巨细胞瘤(non-giant cell tumor of bone,NGCTB)(包含14例动脉瘤样骨囊肿、16例软骨母细胞瘤和53例非骨化性纤维瘤)患者的样本和病历资料,采用免疫组织化学EliVision法检测H3.3G34W、p63及SATB2的表达情况。通过χ^(2)检验判断H3.3G34W、p63及SATB2的阳性率在各组间是否存在统计学差异;通过Logistic回归分析建立包括H3.3G34W、p63及SATB2的联合诊断模型,通过受试者工作特征(ROC)曲线分析评价模型的诊断价值。结果H3.3G34W、p63及SATB2在GCTB组中阳性率分别为81.5%、90.7%、92.6%;在NGCTB组中阳性率分别为2.4%、28.9%、62.7%。与NGCTB组相比,GCTB组患者年龄显著较大[(41.222±14.849)vs.(16.566±9.439);P<0.001],女性比男性患病率更高(51.9%vs.48.1%,P<0.001)。与NGCTB组相比,GCTB组中H3.3G34W(81.5%vs.2.4%,P<0.001);p63(90.7%vs.28.9%,P<0.001)和SATB2(92.6%vs.62.7%,P<0.001)的阳性率更高。单因素Logistic回归分析构建单因素预测模型,同时行ROC曲线分析,表明年龄(AUC=92.9%,P<0.001)、性别(AUC=64.5%,P=0.004)、H3.3G34W阳性率(AUC=89.5%,P<0.001)、p63阳性率(AUC=80.9%,P<0.001)、SATB2阳性率(AUC=65.0%,P=0.003)是GCTB诊断的独立预测因素。进一步的多因素Logistic回归分析构建混合预测模型,并行ROC曲线分析,发现混合模型展现出比单因素模型更好的预测价值(AUC=98.4%,P<0.001)。结论H3.3G34W、p63及SATB2是有效诊断GCTB的分子标记物,且三者联合应用更能提高GCTB的诊断预测效能。
基金supported by the National Natural Science Foundation of China,Nos. 31872765 and 81790642 (to ZFW)a grant from the Shanghai Municipal Science and Technology Major Project,No. 2018SHZDZX01 (to ZFW)+1 种基金ZJ LabShanghai Center for Brain Science and Brain-Inspired Technology
文摘Retinal ganglion cell apoptotic death is the main pathological characteristic of glaucoma,which is the leading cause of irreversible blindness.Disruption of Ca^(2+)homeostasis plays an important role in glaucoma.Voltage-gated Ca^(2+)channel blockers have been shown to improve vision in patients with glaucoma.However,whether and how voltage-gated Ca^(2+)channels are involved in retinal ganglion cell apoptotic death are largely unknown.In this study,we found that total Ca^(2+)current densities in retinal ganglion cells were reduced in a rat model of chronic ocular hypertension experimental glaucoma,as determined by whole-cell patch-clamp electrophysiological recordings.Further analysis showed that L-type Ca^(2+)currents were downregulated while T-type Ca^(2+)currents were upregulated at the later stage of glaucoma.Western blot assay and immunofluorescence experiments confirmed that expression of the Ca_(V)1.2 subunit of L-type Ca^(2+)channels was reduced and expression of the Ca_(V)3.3 subunit of T-type Ca^(2+)channels was increased in retinas of the chronic ocular hypertension model.Soluble tumor necrosis factor-α,an important inflammatory factor,inhibited the L-type Ca^(2+)current of isolated retinal ganglion cells from control rats and enhanced the T-type Ca^(2+)current.These changes were blocked by the tumor necrosis factor-αinhibitor XPro1595,indicating that both types of Ca^(2+)currents may be mediated by soluble tumor necrosis factor-α.The intracellular mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and nuclear factor kappa-B signaling pathway mediate the effects of tumor necrosis factor-α.TUNEL assays revealed that mibefradil,a T-type calcium channel blocker,reduced the number of apoptotic retinal ganglion cells in the rat model of chronic ocular hypertension.These results suggest that T-type Ca^(2+)channels are involved in disrupted Ca^(2+)homeostasis and apoptosis of retinal ganglion cells in glaucoma,and application of T-type Ca^(2+)channel blockers,especially a specific CaV3.3 blocker,may be a potential strategy for the treatment of glaucoma.
文摘通过扫描电镜/能谱、X射线衍射以及金相分析,针对含0.3%Fe(质量分数)的Al Mg Si Cu铝合金,研究了Mn含量对其结晶相的影响。研究表明:合金在铸造过程中形成的结晶相为Al1.9CuMg4.1Si3.3,Al5(FeMn)Si,Al8(FeMn)2Si以及少量的Mg2Si;增大含Mn量,合金中AlFeMnSi型结晶相数量增多;对合金进行均匀化处理时,Al1.9CuMg4.1Si3.3相完全溶解,发生Al5(FeMn)Si向Al8(FeMn)2Si相的转变;对合金进行轧制及最终热处理后,结晶相碎化且沿轧向呈纤维状分布,但结晶相的类型不变。
文摘传统的镁熔液第一气泡检测结果存在较大滞后性,且以Matlab为代表的检测工具又受限于工程应用,本文提出一种基于DSP检测弱小目标的新思路,构建了一个以DM642为主处理器的嵌入式图像处理系统平台,并利用C语言在DSP集成开发软件Code Composer Studio 3.3上进行算法编程,实现了镁熔液第一气泡的检测。实验结果验证了镁熔液第一气泡检测方法在实际应用中的可行性。
