The method of plasma-spray coating of hy- droxyapatite （HA） onto pure titanium has been demon- strated to be effective to enhance the osteogenic differentiation and accelerate bone regeneration. Yet it is still a bi...The method of plasma-spray coating of hy- droxyapatite （HA） onto pure titanium has been demon- strated to be effective to enhance the osteogenic differentiation and accelerate bone regeneration. Yet it is still a big challenge to figure out the interplay among im- plant surface properties, adsorbed proteins and cell-surface interactions. In this study, the plasma-sprayed HA-coated titanium （HA-Ti） surface was compared with the titanium substrate in terms of protein adsorption, cell adhesion and differentiation. The phase composition, wettability and to- pography were characterized. Compared to the Ti substrate, the HA-Ti had a smaller water contact angle, but larger micro-scale roughness, and showed a poorer ability to ad- sorb fibronectin （Fn）, bovine serum albumin （BSA） and serum proteins. However, it could adsorb larger amount of recombinant human bone morphogenetic protein 2 （BMP- 2）. The osteoblasts and bone marrow mesenchymal stem cells （BMSCs） tended to adhere on the Ti substrate. By contrast, the BMSCs cultured on the HA-Ti showed a stronger tendency toward osteogenesis differentiation.展开更多
基金supported by the National Basic Research Program of China(2011CB606203)the National Natural Science Foundation of China(21434006,21374097)
文摘The method of plasma-spray coating of hy- droxyapatite （HA） onto pure titanium has been demon- strated to be effective to enhance the osteogenic differentiation and accelerate bone regeneration. Yet it is still a big challenge to figure out the interplay among im- plant surface properties, adsorbed proteins and cell-surface interactions. In this study, the plasma-sprayed HA-coated titanium （HA-Ti） surface was compared with the titanium substrate in terms of protein adsorption, cell adhesion and differentiation. The phase composition, wettability and to- pography were characterized. Compared to the Ti substrate, the HA-Ti had a smaller water contact angle, but larger micro-scale roughness, and showed a poorer ability to ad- sorb fibronectin （Fn）, bovine serum albumin （BSA） and serum proteins. However, it could adsorb larger amount of recombinant human bone morphogenetic protein 2 （BMP- 2）. The osteoblasts and bone marrow mesenchymal stem cells （BMSCs） tended to adhere on the Ti substrate. By contrast, the BMSCs cultured on the HA-Ti showed a stronger tendency toward osteogenesis differentiation.
