为掌握江苏湖泊鳙群体的遗传资源状况,利用COⅠ基因序列研究了6个湖泊(太湖、滆湖、长荡湖、高邮湖、洪泽湖和骆马湖)鳙群体的遗传多样性及遗传结构。结果显示,在分析的630 bp COⅠ基因片段中,碱基A+T含量(55.3%)高于C+G含量(44.7%)。22...为掌握江苏湖泊鳙群体的遗传资源状况,利用COⅠ基因序列研究了6个湖泊(太湖、滆湖、长荡湖、高邮湖、洪泽湖和骆马湖)鳙群体的遗传多样性及遗传结构。结果显示,在分析的630 bp COⅠ基因片段中,碱基A+T含量(55.3%)高于C+G含量(44.7%)。223条COⅠ序列检测出14个变异位点,定义9个单倍型,6个群体的单倍型多样性为0.494~0.665,核苷酸多样性为0.0009~0.0019,总体单倍型多样性和核苷酸多样性分别为0.576和0.0012,具有高单倍型多样性和低核苷酸多样性的特点,表明6个湖泊鳙群体遗传多样性较低。分子方差分析结果显示,遗传变异主要来自于群体内(99.39%),总遗传分化指数为0.0061,群体间遗传分化指数为-0.0228~0.0445,遗传距离为0.0012~0.0027,表明群体间没有明显的遗传分化。单倍型系统发育树和网络结构图显示,单倍型在6个群体中混杂分布,未形成特定的湖泊分支。中性检验和歧点分布分析表明,整个鳙群体经历过显著的种群扩张。结果表明,江苏湖泊鳙群体的遗传多样性较低,遗传结构趋于同质化,需要采取措施提高鳙的遗传多样性。展开更多
Objective:To determine the effect of Lentinula edodes extract on ultraviolet(UV)A and UVB-induced changes in matrix metalloproteinase(MMP)and type I procollagen expression using human immortalized HaCaT keratinocytes....Objective:To determine the effect of Lentinula edodes extract on ultraviolet(UV)A and UVB-induced changes in matrix metalloproteinase(MMP)and type I procollagen expression using human immortalized HaCaT keratinocytes.Methods:Lentinula edodes ethanol extract(LEE)was obtained by extraction with 80%ethanol for 4 h at 80℃.Effect of LEE on UVinduced alteration on the expression and production of MMPs and type I procollagen in keratinocytes was investigated using ELISA,RT-PCR,and Western blotting assay.To determine the underlying mechanism of LEE-mediated effects,mitogen-activated protein kinase(MAPK)and activator protein 1 signaling pathways were analysed by Western blotting assay.Results:LEE significantly inhibited the expression of MMP-1 and MMP-9 and increased the expression of type I procollagen in UVA and UVB-irradiated HaCaT keratinocytes.The phosphorylation levels of p38 were significantly inhibited by LEE whereas it did not affect c-Jun N-terminal kinase and extracellular signal-regulated kinase phosphorylation.Suppression of p38 phosphorylation was also accompanied by downregulation of UVA and UVB-induced increase in c-Fos.Conclusions:LEE effectively inhibits the expression of MMP-1 and MMP-9 and increases type I procollagen production through the p38 MAPK/c-Fos signaling pathway in UVA and UVB-irradiated HaCaT keratinocytes.This findings suggest that Lentinula edodes may be developed as a cosmetic material to suppress UV exposuremediated skin aging.展开更多
文摘为掌握江苏湖泊鳙群体的遗传资源状况,利用COⅠ基因序列研究了6个湖泊(太湖、滆湖、长荡湖、高邮湖、洪泽湖和骆马湖)鳙群体的遗传多样性及遗传结构。结果显示,在分析的630 bp COⅠ基因片段中,碱基A+T含量(55.3%)高于C+G含量(44.7%)。223条COⅠ序列检测出14个变异位点,定义9个单倍型,6个群体的单倍型多样性为0.494~0.665,核苷酸多样性为0.0009~0.0019,总体单倍型多样性和核苷酸多样性分别为0.576和0.0012,具有高单倍型多样性和低核苷酸多样性的特点,表明6个湖泊鳙群体遗传多样性较低。分子方差分析结果显示,遗传变异主要来自于群体内(99.39%),总遗传分化指数为0.0061,群体间遗传分化指数为-0.0228~0.0445,遗传距离为0.0012~0.0027,表明群体间没有明显的遗传分化。单倍型系统发育树和网络结构图显示,单倍型在6个群体中混杂分布,未形成特定的湖泊分支。中性检验和歧点分布分析表明,整个鳙群体经历过显著的种群扩张。结果表明,江苏湖泊鳙群体的遗传多样性较低,遗传结构趋于同质化,需要采取措施提高鳙的遗传多样性。
基金supported by the Ministry of Trade,Industry&Energy,Korea Institute for Advancement of Technology and Busan Institute For Regional Program Evaluation through the Encouragement Program for the Social and Economic Innovation Growth(project number,P0008724)
文摘Objective:To determine the effect of Lentinula edodes extract on ultraviolet(UV)A and UVB-induced changes in matrix metalloproteinase(MMP)and type I procollagen expression using human immortalized HaCaT keratinocytes.Methods:Lentinula edodes ethanol extract(LEE)was obtained by extraction with 80%ethanol for 4 h at 80℃.Effect of LEE on UVinduced alteration on the expression and production of MMPs and type I procollagen in keratinocytes was investigated using ELISA,RT-PCR,and Western blotting assay.To determine the underlying mechanism of LEE-mediated effects,mitogen-activated protein kinase(MAPK)and activator protein 1 signaling pathways were analysed by Western blotting assay.Results:LEE significantly inhibited the expression of MMP-1 and MMP-9 and increased the expression of type I procollagen in UVA and UVB-irradiated HaCaT keratinocytes.The phosphorylation levels of p38 were significantly inhibited by LEE whereas it did not affect c-Jun N-terminal kinase and extracellular signal-regulated kinase phosphorylation.Suppression of p38 phosphorylation was also accompanied by downregulation of UVA and UVB-induced increase in c-Fos.Conclusions:LEE effectively inhibits the expression of MMP-1 and MMP-9 and increases type I procollagen production through the p38 MAPK/c-Fos signaling pathway in UVA and UVB-irradiated HaCaT keratinocytes.This findings suggest that Lentinula edodes may be developed as a cosmetic material to suppress UV exposuremediated skin aging.
