牡丹非特异性脂质转移蛋白基因PsLTP的生物信息学分析

[目的]了解牡丹非特异性脂质转移蛋白基因LTP的基因特征及其编码蛋白的生物学特性,探索其生物功能。[方法]运用Blast、ORF-Finder、ProtParam、SignalP 4.1、ProtScale和TMHMM 2.0 Server等生物信息学软件,分析牡丹nsLTP基因序列及其编码蛋白的基本理化性质、信号肽、磷酸化位点、保守结构域等,并运用DANMAN、MEGA软件进行多序列比对和系统发育树构建。[结果]牡丹nsLTP基因(NCBI登录号为:JZ840269.1)序列全长为685 bp,开放阅读框长为354 bp,编码蛋白包含117个氨基酸,理论等电点为8.93,为不稳定性蛋白。牡丹nsLTP蛋白为疏水性蛋白,含有信号肽但不含跨膜结构域,NetPho Server预测其含有8个丝氨酸和3个酪氨酸磷酸化位点及5个苏氨酸磷酸化位点。PsnsLTP蛋白具有植物LTP蛋白典型结构,即4个α-螺旋,4对二硫键,1个可结合和容纳脂质分子的疏水结构。多序列比对及系统发育树分析显示,PsLTP蛋白与绒毛烟草LTP蛋白(XP_009608993.1)的相识度62.7%,且进化树聚为同一小分支,遗传距离较近。[结论]通过对牡丹nsLTP基因特性及蛋白结构研究,系统性研究了牡丹nsLTP基因的生物信息学特性,为牡丹抗病抗逆功能基因的研究提供了依据,也为其他植物nsLTP基因研究提供了参考。

不同宽长比的柔性LTPS TFT的电应力可靠性

为了研究不同宽长比的柔性低温多晶硅薄膜晶体管(LTPS TFT)的电应力可靠性,测试了器件的I-V特性,以表征器件在强电场直流应力下由于自热效应和热载流子效应带来的电学性能退化。通过瞬态电流法表征了器件在强电场直流应力下的时间常数谱,并对其产生的新陷阱进行定位,分析了产生陷阱的内在机理。结果表明,在相同的强电场直流应力下宽长比为3/2.5的器件,其电学参数变化最大,自热效应以及热载流子效应带来的影响也最大。自热效应导致器件性能退化的主要原因是较大的栅源电压导致Si/SiO2界面处和栅氧化层中的陷阱增多,而热载流子效应导致器件性能退化的主要原因则是由于较大的漏源电压使得漏极晶界陷阱态密度急剧升高。

Corneal collagen cross-linking in patients with keratoconus from the Dresden protocol to customized solutions:theoretical basis

Keratoconus is an ectatic condition characterized by gradual corneal thinning,corneal protrusion,progressive irregular astigmatism,corneal fibrosis,and visual impairment.The therapeutic options regarding improvement of visual function include glasses or soft contact lenses correction for initial stages,gas-permeable rigid contact lenses,scleral lenses,implantation of intrastromal corneal ring or corneal transplants for most advanced stages.In keratoconus cases showing disease progression corneal collagen crosslinking(CXL)has been proven to be an effective,minimally invasive and safe procedure.CXL consists of a photochemical reaction of corneal collagen by riboflavin stimulation with ultraviolet A radiation,resulting in stromal crosslinks formation.The aim of this review is to carry out an examination of CXL methods based on theoretical basis and mathematical models,from the original Dresden protocol to the most recent developments in the technique,reporting the changes proposed in the last 15y and examining the advantages and disadvantages of the various treatment protocols.Finally,the limits of non-standardized methods and the perspectives offered by a customization of the treatment are highlighted.

Designing Proportional-Integral Consensus Protocols for Second-Order Multi-Agent Systems Using Delayed and Memorized State Information

This paper is concerned with consensus of a secondorder linear time-invariant multi-agent system in the situation that there exists a communication delay among the agents in the network.A proportional-integral consensus protocol is designed by using delayed and memorized state information.Under the proportional-integral consensus protocol,the consensus problem of the multi-agent system is transformed into the problem of asymptotic stability of the corresponding linear time-invariant time-delay system.Note that the location of the eigenvalues of the corresponding characteristic function of the linear time-invariant time-delay system not only determines the stability of the system,but also plays a critical role in the dynamic performance of the system.In this paper,based on recent results on the distribution of roots of quasi-polynomials,several necessary conditions for Hurwitz stability for a class of quasi-polynomials are first derived.Then allowable regions of consensus protocol parameters are estimated.Some necessary and sufficient conditions for determining effective protocol parameters are provided.The designed protocol can achieve consensus and improve the dynamic performance of the second-order multi-agent system.Moreover,the effects of delays on consensus of systems of harmonic oscillators/double integrators under proportional-integral consensus protocols are investigated.Furthermore,some results on proportional-integral consensus are derived for a class of high-order linear time-invariant multi-agent systems.

A Formal Model for Analyzing Fair Exchange Protocols Based on Event Logic

Fair exchange protocols play a critical role in enabling two distrustful entities to conduct electronic data exchanges in a fair and secure manner.These protocols are widely used in electronic payment systems and electronic contract signing,ensuring the reliability and security of network transactions.In order to address the limitations of current research methods and enhance the analytical capabilities for fair exchange protocols,this paper proposes a formal model for analyzing such protocols.The proposed model begins with a thorough analysis of fair exchange protocols,followed by the formal definition of fairness.This definition accurately captures the inherent requirements of fair exchange protocols.Building upon event logic,the model incorporates the time factor into predicates and introduces knowledge set axioms.This enhancement empowers the improved logic to effectively describe the state and knowledge of protocol participants at different time points,facilitating reasoning about their acquired knowledge.To maximize the intruder’s capabilities,channel errors are translated into the behaviors of the intruder.The participants are further categorized into honest participants and malicious participants,enabling a comprehensive evaluation of the intruder’s potential impact.By employing a typical fair exchange protocol as an illustrative example,this paper demonstrates the detailed steps of utilizing the proposed model for protocol analysis.The entire process of protocol execution under attack scenarios is presented,shedding light on the underlying reasons for the attacks and proposing corresponding countermeasures.The developedmodel enhances the ability to reason about and evaluate the security properties of fair exchange protocols,thereby contributing to the advancement of secure network transactions.

Falcon Optimization Algorithm-Based Energy Efficient Communication Protocol for Cluster-Based Vehicular Networks

Rapid development in Information Technology(IT)has allowed several novel application regions like large outdoor vehicular networks for Vehicle-to-Vehicle(V2V)transmission.Vehicular networks give a safe and more effective driving experience by presenting time-sensitive and location-aware data.The communication occurs directly between V2V and Base Station(BS)units such as the Road Side Unit(RSU),named as a Vehicle to Infrastructure(V2I).However,the frequent topology alterations in VANETs generate several problems with data transmission as the vehicle velocity differs with time.Therefore,the scheme of an effectual routing protocol for reliable and stable communications is significant.Current research demonstrates that clustering is an intelligent method for effectual routing in a mobile environment.Therefore,this article presents a Falcon Optimization Algorithm-based Energy Efficient Communication Protocol for Cluster-based Routing(FOA-EECPCR)technique in VANETS.The FOA-EECPCR technique intends to group the vehicles and determine the shortest route in the VANET.To accomplish this,the FOA-EECPCR technique initially clusters the vehicles using FOA with fitness functions comprising energy,distance,and trust level.For the routing process,the Sparrow Search Algorithm(SSA)is derived with a fitness function that encompasses two variables,namely,energy and distance.A series of experiments have been conducted to exhibit the enhanced performance of the FOA-EECPCR method.The experimental outcomes demonstrate the enhanced performance of the FOA-EECPCR approach over other current methods.

Hyperbolic Tangent Function-Based Protocols for Global/Semi-Global Finite-Time Consensus of Multi-Agent Systems

This paper investigates the problem of global/semi-global finite-time consensus for integrator-type multi-agent sys-tems.New hyperbolic tangent function-based protocols are pro-posed to achieve global and semi-global finite-time consensus for both single-integrator and double-integrator multi-agent systems with leaderless undirected and leader-following directed commu-nication topologies.These new protocols not only provide an explicit upper-bound estimate for the settling time,but also have a user-prescribed bounded control level.In addition,compared to some existing results based on the saturation function,the pro-posed approach considerably simplifies the protocol design and the stability analysis.Illustrative examples and an application demonstrate the effectiveness of the proposed protocols.

Protocol for lower back pain management: Insights from the French healthcare system

In this editorial we comment on the article published in a recent issue of the World Journal of Clinical Cases.This article described a novel ultrasound-guided lateral recess block approach in treating a patient with lateral recess stenosis.The impact of spinal pain-related disability extends significantly,causing substantial human suffering and medical costs.Each county has its preferred treatment strategies for spinal pain.Here,we explore the lower back pain(LBP)treatment algorithm recommended in France.The treatment algorithm for LBP recommended by the French National Authority for Health emphasizes early patient activity and minimal medication use.It encourages the continuation of daily activities,limits excessive medication and spinal injections,and incorporates psychological assessments and non-pharmacological therapies for chronic cases.However,the algorithm may not aggressively address acute pain in the early stages,potentially delaying relief and increasing the risk of chronicity.Additionally,the recommended infiltrations primarily involve caudal epidural steroid injections,with limited consideration for other injection procedures,such as transforaminal or interlaminar epidural steroid injections.The fixed follow-up timeline may not accommodate patients who do not respond to initial treatment or experience intense pain,potentially delaying the exploration of alternative therapies.Despite these limitations,understanding the strengths and weaknesses of the French approach could inform adaptations in LBP treatment strategies globally,potentially enhancing patient outcomes and satisfaction across diverse healthcare systems.

Safety and effectiveness of butorphanol in epidural labor analgesia:A protocol for a systematic review and meta-analysis

BACKGROUND Epidural analgesia is the most effective analgesic method during labor.Butorphanol administered epidurally has been shown to be a successful analgesic method during labor.However,no comprehensive study has examined the safety and efficacy of using butorphanol as an epidural analgesic during labor.AIM To assess butorphanol's safety and efficacy for epidural labor analgesia.METHODS The PubMed,Cochrane Library,EMBASE,Web of Science,China National Knowledge Infrastructure,and Google Scholar databases will be searched from inception.Other types of literature,such as conference abstracts and references to pertinent reviews,will also be reviewed.We will include randomized controlled trials comparing butorphanol with other opioids combined with local anesthetics for epidural analgesia during labor.There will be no language restrictions.The primary outcomes will include the visual analog scale score for the first stage of labor,fetal effects,and Apgar score.Two independent reviewers will evaluate the full texts,extract data,and assess the risk of bias.Publication bias will be evaluated using Egger's or Begg's tests as well as visual analysis of a funnel plot,and heterogeneity will be evaluated using the Cochran Q test,P values,and I2 values.Meta-analysis,subgroup analysis,and sensitivity analysis will be performed using RevMan software version 5.4.This protocol was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA)Protocols statement,and the PRISMA statement will be used for the systematic review.RESULTS This study provides reliable information regarding the safety and efficacy of using butorphanol as an epidural analgesic during labor.CONCLUSION To support clinical practice and development,this study provides evidence-based findings regarding the safety and efficacy of using butorphanol as an epidural analgesic during labor.

Exploration of standard dosage for GnRH antagonist protocol and dosage adjustments after premature luteinizing hormone surge

The gonadotropin-releasing hormone (GnRH) antagonist protocol has emerged as an efficacious alternative to the GnRH agonist protocol for controlled ovarian hyperstimulation (COH) during in vitro fertilization (IVF) cycles, and has been demonstrated applicability in infertile female patients with diverse ovarian responses. While the clinical implementation of the antagonist COH protocol has achieved widespread consensus, opportunities for refinement persist. Therefore, this review article focuses on the advantages and disadvantages of GnRH antagonist protocol, the selection of optimal standard doses, and the strategies for adjusting antagonist doses after the premature luteinizing hormone (LH) surge, aiming to provide more reasonable and scientific recommendations for the application of this scheme.

Priority Based Energy Efficient MAC Protocol by Varying Data Ratefor Wireless Body Area Network

Wireless Body Area Network(WBAN)is a cutting-edge technology that is being used in healthcare applications to monitor critical events in the human body.WBAN is a collection of in-body and on-body sensors that monitor human physical parameters such as temperature,blood pressure,pulse rate,oxygen level,body motion,and so on.They sense the data and communicate it to the Body Area Network(BAN)Coordinator.The main challenge for the WBAN is energy consumption.These issues can be addressed by implementing an effective Medium Access Control(MAC)protocol that reduces energy consumption and increases network lifetime.The purpose of the study is to minimize the energy consumption and minimize the delay using IEEE 802.15.4 standard.In our proposed work,if any critical events have occurred the proposed work is to classify and prioritize the data.We gave priority to the highly critical data to get the Guarantee Tine Slots(GTS)in IEEE 802.15.4 standard superframe to achieve greater energy efficiency.The proposed MAC provides higher data rates for critical data based on the history and current condition and also provides the best reliable service to high critical data and critical data by predicting node similarity.As an outcome,we proposed a MAC protocol for Variable Data Rates(MVDR).When compared to existing MAC protocols,the MVDR performed very well with low energy intake,less interruption,and an enhanced packet-sharing ratio.

A Generic and Reliable Land Acquisition Protocol Implementation for Sub-Saharan Africa Countries

Land acquisition is subject of many frauds worldwide. In Africa countries, land acquisition frauds are a real curse. Main reasons of the high land acquisition frauds in Africa are a lack of a reliable land acquisition protocol implementation, no centralized information system that records land transactions and integrates all actors, lack of education about the acquisition protocol and lack of applied sanctions in case of frauds. As of sanctions, the issue is bound to the corruption plague in Africa. Authorities that are supposed to rule the laws are not well paid and are part of the corruption scheme. Most frequent frauds are multiple sales of the same land, falsified land title, false owner identification. In Africa, a land belongs generally to the first residents of the area. To claim ownership of a land, an owner should be recognized by the rural or city authorities. To achieve this recognition, he must obtain from those authorities an attribution letter. The author proposed a complete and reliable land acquisition protocol named ACLAP (Africa Countries Land Acquisition Protocol). The purpose of this paper is to explain the main components of the protocol implementation and show its efficiency when confronting fraud threats. In this paper, a Generic and Reliable Land Acquisition Protocol Implementation in Sub-Saharan Africa countries context is proposed. The platform keeps relevant land transaction information for administrators, managers, buyers, sellers, or for people consultation purpose. The application may be used in village, city, department, region, or country for land management decision making.

Clinical comprehensive treatment protocol for managing diabetic foot ulcers:A retrospective cohort study

BACKGROUND Diabetic foot ulcers(DFUs)are a common complication of diabetes,often leading to severe infections,amputations,and reduced quality of life.The current standard treatment protocols for DFUs have limitations in promoting efficient wound healing and preventing complications.A comprehensive treatment approach targeting multiple aspects of wound care may offer improved outcomes for patients with DFUs.The hypothesis of this study is that a comprehensive treatment protocol for DFUs will result in faster wound healing,reduced amputation rates,and improved overall patient outcomes compared to standard treatment protocols.AIM To compare the efficacy and safety of a comprehensive treatment protocol for DFUs with those of the standard treatment protocol.METHODS This retrospective study included 62 patients with DFUs,enrolled between January 2022 and January 2024,randomly assigned to the experimental(n=32)or control(n=30)group.The experimental group received a comprehensive treatment comprising blood circulation improvement,debridement,vacuum sealing drainage,recombinant human epidermal growth factor and anti-inflammatory dressing,and skin grafting.The control group received standard treatment,which included wound cleaning and dressing,antibiotics administration,and surgical debridement or amputation,if necessary.Time taken to reduce the white blood cell count,number of dressing changes,wound healing rate and time,and amputation rate were assessed.RESULTS The experimental group exhibited significantly better outcomes than those of the control group in terms of the wound healing rate,wound healing time,and amputation rate.Additionally,the comprehensive treatment protocol was safe and well tolerated by the patients.CONCLUSION Comprehensive treatment for DFUs is more effective than standard treatment,promoting granulation tissue growth,shortening hospitalization time,reducing pain and amputation rate,improving wound healing,and enhancing quality of life.

Cell replacement with stem cell-derived retinal ganglion cells from different protocols

Glaucoma,characterized by a degenerative loss of retinal ganglion cells,is the second leading cause of blindness worldwide.There is currently no cure for vision loss in glaucoma because retinal ganglion cells do not regenerate and are not replaced after injury.Human stem cell-derived retinal ganglion cell transplant is a potential therapeutic strategy for retinal ganglion cell degenerative diseases.In this review,we first discuss a 2D protocol for retinal ganglion cell differentiation from human stem cell culture,including a rapid protocol that can generate retinal ganglion cells in less than two weeks and focus on their transplantation outcomes.Next,we discuss using 3D retinal organoids for retinal ganglion cell transplantation,comparing cell suspensions and clusters.This review provides insight into current knowledge on human stem cell-derived retinal ganglion cell differentiation and transplantation,with an impact on the field of regenerative medicine and especially retinal ganglion cell degenerative diseases such as glaucoma and other optic neuropathies.

BDSec:Security Authentication Protocol for BeiDou-II Civil Navigation Message

Due to the lack of authentication mechanism in BeiDou navigation satellite system(BDS),BD-II civil navigation message(BDII-CNAV)are vulnerable to spoofing attack and replay attack.To solve this problem,we present a security authentication protocol,called as BDSec,which is designed by using China’s cryptography Shangyong Mima(SM)series algorithms,such as SM2/4/9 and Zu Chongzhi(ZUC)algorithm.In BDSec protocol,both of BDII-CNAV and signature information are encrypted using the SM4 algorithm(Symmetric encryption mechanism).The encrypted result is used as the subject authentication information.BDSec protocol applies SM9 algorithm(Identity-based cryptography mechanism)to protect the integrity of the BDII-CNAV,adopts the SM2 algorithm(Public key cryptosystem)to guarantee the confidentiality of the important session information,and uses the ZUC algorithm(Encryption and integrity algorithm)to verify the integrity of the message authentication serial number and initial information and the information in authentication initialization sub-protocol respectively.The results of the SVO logic reasoning and performance analysis show that BDSec protocol meets security requirements for the dual user identity authentication in BDS and can realize the security authentication of BDII-CNAV.

深空通信中LTP协议选窗概率优化

为满足深空延迟/容忍网络(DTN)中不同类型不同权重数据差异化传输的需求,提出一种基于自适应遗传算法的选窗概率函数优化方法。该方法以红绿(重要、不重要)数据误码率为适应度函数进行交叉变异与全局优化,使重要数据被大概率选中并以较高的正确率传输,不重要数据不被遗漏并在预期误码率范围内传输。将其应用在DTN网络传输层LTP协议的扩展窗不等差喷泉码编码中,通过与传统遗传算法的仿真结果对比表明自适应遗传的优化算法能降低深空通信中重要数据的误码率和信道总误码率,满足差异性数据传输的要求,减少因误码率高而导致的信息重传,提高数据传输效率,节省信道资源。

A fixed clustering protocol based on random relay strategy for EHWSN

In Energy Harvesting Wireless Sensor Networks(EHWSN),the communication protocol will directly affect the final performance of the network,so it is necessary to study the communication protocol based on EHWSN.In this paper,for the low-cost fixed clustering problem,a fixed clustering protocol RRCEH is based on random relaying.Our proposed RRCEH abandons the inefficient inter-cluster communication method of the traditional fixed clustering protocol.To coordinate the data upload of the cluster head,RRCEH allocates different random relay vectors to each ring area of the network,and combines all the random relay vectors into a random relay matrix of RRCEH.In each communication round,the cluster head node randomly selects its relay target node to send data according to the probability distribution in the random relay vector in the area.For two different cluster head configuration scenarios,by optimizing the random relay matrix,RRCEH can effectively reduce the network's configuration requirements for cluster head energy harvesting capability,thus reducing the deployment cost of EHWSN.

Coati Optimization-Based Energy Efficient Routing Protocol for Unmanned Aerial Vehicle Communication

With the flexible deployment and high mobility of Unmanned Aerial Vehicles(UAVs)in an open environment,they have generated con-siderable attention in military and civil applications intending to enable ubiquitous connectivity and foster agile communications.The difficulty stems from features other than mobile ad-hoc network(MANET),namely aerial mobility in three-dimensional space and often changing topology.In the UAV network,a single node serves as a forwarding,transmitting,and receiving node at the same time.Typically,the communication path is multi-hop,and routing significantly affects the network’s performance.A lot of effort should be invested in performance analysis for selecting the optimum routing system.With this motivation,this study modelled a new Coati Optimization Algorithm-based Energy-Efficient Routing Process for Unmanned Aerial Vehicle Communication(COAER-UAVC)technique.The presented COAER-UAVC technique establishes effective routes for communication between the UAVs.It is primarily based on the coati characteristics in nature:if attacking and hunting iguanas and escaping from predators.Besides,the presented COAER-UAVC technique concentrates on the design of fitness functions to minimize energy utilization and communication delay.A varied group of simulations was performed to depict the optimum performance of the COAER-UAVC system.The experimental results verified that the COAER-UAVC technique had assured improved performance over other approaches.

基于LTP的糖尿病智能问答系统的研究与实现

广泛收集健康网站用户实际提出的糖尿病相关问题,以LTP自然语言处理平台为基础对问句进行综合分析,建立糖尿病基本诊疗、指标、用药、饮食、保健品、预防及疑问(判断)词汇等方面的分类词典.应用词语匹配、依存句法分析、相关词语组合分析等方法进行语义关键词提取.在此基础上进行问题分类理解,同时结合问题分类及相关的决策树构建糖尿病的相关知识图谱及问题的判断流程,实现了问题与回答的有效匹配.系统实际问答测试结果是识别率为91.3%、准确率为83.6%.

Experimental investigation on degradation mechanism of membrane electrode assembly at different humidity under automotive protocol

Humidity can affect the attenuation of MEA(membrane electrode assembly), however, the relationship between humidity and MEA decays is complex and ambiguous in realistic application. Herein, we design a simulating automotive protocol, performed on five single fuel cells under RH(relative humidity) 100%,RH 80%, RH 64%, and RH 40%, RH 10%, respectively, to study the relationship of MEA decays and humidity and suggest optimized humidity range to extend the durability. With the electrochemical impedance spectroscopy, cyclic voltammetry, X-ray fluorescence, X-ray diffraction, transmission electron microscope, X-ray photoelectron spectroscopy, the four degradation mechanisms about catalyst layer, including Pt dissolution, Pt coarsening, carbon corrosion and ionomer degradation, are observed. Pt coarsening and carbon corrosion are accelerated by higher water content at high humidity. Ionomer degradation and Pt dissolution are enhanced in low humidity. With the linear sweep voltammetry, ion chromatography,nuclear magnetic resonance, tensile test and scan electron microscope, chemical and mechanical degradation in proton exchange membrane are all observed in these five fuels. Chemical degradation, characterized by membrane thinning and more fluoride loss, occurred markedly in RH 10%. Mechanical degradation, characterized by the non-uniformity thickness and bad mechanical properties, is more pronounced in RH 100%, RH 80%, RH 64%. These two degradations are in a moderate level in RH 40%. The research suggests that the RH range from 64% to 40% is conductive to mitigate the degradation of MEAs operated in automotive applications.