[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best ...[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best extraction conditions of total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. were determined,and reducing ability of the extracted total flavonoids and its DPPH and ABTS scavenging abilities were explored. [Results] The best extraction technology conditions: solid-liquid ratio of 1∶ 50,ethanol concentration of 50%,extraction time of 2. 5 h,extraction temperature of 85 ℃,two-time extraction. By detecting DPPH and ABTS scavenging abilities of total flavonoids,the anti-oxidation activity of the total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. was analyzed and evaluated. [Conclusions]Fruits and leaves of P. mume Sieb. et Zucc. had a certain in vitro anti-oxidation activity,and heat reflux extraction method of its total flavonoids had high extraction rate and simple and convenient operation,which had some practical value.展开更多
Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research ...Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research Center of China Mei Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Neis (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mei Cultivars. But there were still several exceptions: 1) the Guhong Chuizhi inserted between the Tiaoxue Chuizhi and the Danfen Chuizhi; 2) the Wufu Chuizhi kept off the Pink Pendant Form, and the Moshan Chuizhi was removed from Viridiflora Pendant Form; 3) the Danbi Chuizhi and the Shuangbi Chuizhi of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form.展开更多
基金Supported by 2014 Sichuan Science and Technology Support Plan Program(2014SZ0131)Education Innovation Project of Southwest Minzu University(2015)
文摘[Objectives]To optimize the extraction technology of total flavonoids component,and investigate its in vitro anti-oxidation activity.[Methods]The single factor was inspected firstly. By orthogonal experiment,the best extraction conditions of total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. were determined,and reducing ability of the extracted total flavonoids and its DPPH and ABTS scavenging abilities were explored. [Results] The best extraction technology conditions: solid-liquid ratio of 1∶ 50,ethanol concentration of 50%,extraction time of 2. 5 h,extraction temperature of 85 ℃,two-time extraction. By detecting DPPH and ABTS scavenging abilities of total flavonoids,the anti-oxidation activity of the total flavonoids from fruits and leaves of P. mume Sieb. et Zucc. was analyzed and evaluated. [Conclusions]Fruits and leaves of P. mume Sieb. et Zucc. had a certain in vitro anti-oxidation activity,and heat reflux extraction method of its total flavonoids had high extraction rate and simple and convenient operation,which had some practical value.
基金the Natural Science Foundation of Hubei Province P. R. China (Grant No. 2001ABB118)
文摘Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research Center of China Mei Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Neis (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mei Cultivars. But there were still several exceptions: 1) the Guhong Chuizhi inserted between the Tiaoxue Chuizhi and the Danfen Chuizhi; 2) the Wufu Chuizhi kept off the Pink Pendant Form, and the Moshan Chuizhi was removed from Viridiflora Pendant Form; 3) the Danbi Chuizhi and the Shuangbi Chuizhi of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form.
