This study was to molecular identify Prunus necrotic ringspot virus （PNRSV） occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and pa...This study was to molecular identify Prunus necrotic ringspot virus （PNRSV） occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV. An expected cDNA fragment of about 450 bp was amplified from lily, cloned into pGEM-T-easy vector, sequenced, and shared 84.5-99.1% homology with the 27 PNRSV isolates reported previously at the nucleotide level, indicating that lily is a new natural host of PNRSV.展开更多
为明确江苏省扬州市蔷薇科植物中李属坏死环斑病毒(Prunus necrotic ring spot virus,PNRSV)的发生情况,采用ELISA方法,以采集自扬州市不同地点的243份蔷薇科植物叶片样品为试材,测定其中4个分离物的外壳蛋白(CP)编码区序列,结合GenBan...为明确江苏省扬州市蔷薇科植物中李属坏死环斑病毒(Prunus necrotic ring spot virus,PNRSV)的发生情况,采用ELISA方法,以采集自扬州市不同地点的243份蔷薇科植物叶片样品为试材,测定其中4个分离物的外壳蛋白(CP)编码区序列,结合GenBank中已报道的相关序列对其进行多样性和系统发育分析。结果表明,江苏省扬州市蔷薇科植物中PNRSV的检出率为13%;序列分析表明本研究获得的PNRSV分离物与GenBank中其它286个分离物的核苷酸序列一致率为82%~99%;系统发育分析表明PNRSV可以分成4个组,组间具有较为清晰的地理和寄主特异性,其中本研究获得的PNRSV分离物均位于PV32组。总之,PNRSV已经在扬州市发生并流行,应当引起重视。展开更多
文摘This study was to molecular identify Prunus necrotic ringspot virus （PNRSV） occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV. An expected cDNA fragment of about 450 bp was amplified from lily, cloned into pGEM-T-easy vector, sequenced, and shared 84.5-99.1% homology with the 27 PNRSV isolates reported previously at the nucleotide level, indicating that lily is a new natural host of PNRSV.
文摘为明确江苏省扬州市蔷薇科植物中李属坏死环斑病毒(Prunus necrotic ring spot virus,PNRSV)的发生情况,采用ELISA方法,以采集自扬州市不同地点的243份蔷薇科植物叶片样品为试材,测定其中4个分离物的外壳蛋白(CP)编码区序列,结合GenBank中已报道的相关序列对其进行多样性和系统发育分析。结果表明,江苏省扬州市蔷薇科植物中PNRSV的检出率为13%;序列分析表明本研究获得的PNRSV分离物与GenBank中其它286个分离物的核苷酸序列一致率为82%~99%;系统发育分析表明PNRSV可以分成4个组,组间具有较为清晰的地理和寄主特异性,其中本研究获得的PNRSV分离物均位于PV32组。总之,PNRSV已经在扬州市发生并流行,应当引起重视。
