This paper delves into and assesses the differences in biological traits and fruit nutritional composition of 3 varieties of Prunus humilis introduced from 2 different regions.Results showed that(i)the contents of pro...This paper delves into and assesses the differences in biological traits and fruit nutritional composition of 3 varieties of Prunus humilis introduced from 2 different regions.Results showed that(i)the contents of protein,total sugar,and calcium of No.5 variety were higher those of the other 2 varieties,which was recognized as the most suitable variety for fresh fruit;(ii)the comparison of leaf areas index and fruit shape index of 3 varieties shows that the fruit shape index and individual fruit weight of the introduced varieties were higher than those of the control,the diameter of individual fruit of the introduced varieties higher than that of the control indicated that they had a higher fruit hardness,more dry matter accumulation and better fruit quality,and that the introduction region was more suitable for scale-up of P.humilis;(iii)there was a positive correlation with the number of fruiting branches and there was a significant negative correlation with individual fruit weight,while the leaf area had a significant negative correlation with the number of fruiting branches.The introduced varieties can develop normally in the 2 testing areas,notably,No.5 variety performed better than No.4 and No.6.Improving management is the prerequisite for maintaining proper number of fruiting branches and high yield.展开更多
The stem barks of Prunus africana are used in the treatment of the benign prostate. Cameroon is one of the important exporters of the barks. Despite the important measures adopted in Cameroon for sustaining its harves...The stem barks of Prunus africana are used in the treatment of the benign prostate. Cameroon is one of the important exporters of the barks. Despite the important measures adopted in Cameroon for sustaining its harvesting, some many chalenges still remain. The objective of this work is to refine the forest management parameters in relation to P. africana in the regions of Adamaoua and the South-West by developing a volume rate which makes it possible to estimate the production for a new stem. The work took place in two phases: in the South-West in 2010 and in Adamaoua in 2011. Data collection used the semi-direct method, while the cubing equation was deduced by the multiple linear regression method. Two models for volume estimation and three models for mass prediction were developed. The predictive parameters retained are diameter, height of the bole and thickness of the bark. Results show that the average mass of the dry bark for a given P. africana tree species is 27.55 ± 14.44 kg and this varies according to the site. The strong adjusted coefficient of determination (adjusted R2) observed illustrates the reliability of the proposed models. These models provide a reliable tool that can be adopted as a standard in Cameroon for P. africana.展开更多
Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous R...Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous Region of China. The purpose of this study was to determine the genetic structure and genotypic diversity among the different eco-geographical populations. Based on the results from this study, 8 pairs of fluorescent-AFLP primers showed clear electrophoregram and high polymorphism amongst the 64 pairs of EcoR Ⅰ/Mse Ⅰ (Mse Ⅰ - a FAM fluorescent marked primer) primers screened. There was a significant polymorphic difference for the same primer pair in different populations and for the same population with different primer pairs. The percentage of polymorphic loci (P) at species level was higher than Kuche, Hetian, Kashi population levels, respectively. The Nei's gene diversity index (H) and Shannon's information index (I) at species level were higher than those of Kuche, Hetian, and Kashi at population level, respectively. H and I of Kuche population were the highest amongst the three populations. Apricot population genetic diversity was found mainly within the population, Genetic differentiation coefficient between populations (GST) was 0.0882. Gene flow Nm between the populations was 5.1689. Population genetic identity was between 0.9772-0.9811 and genetic distance was between 0.0191-0.0232. These results further indicated that the similarity between populations was higher and the genetic distance between populations was smaller. The UPGMA cluster analysis indicates that the geographical populations at Kuche, Kashi, Hetian were relatively independent Mendelian populations. Concurrently, there was also partial gene exchange between the populations. All the evidences indicated that the genetic diversity in Kuche population was the highest, suggesting that it could be a transition population from wild apricot to cultivated apricot. There were abundant genetic diversities in apricot cultivar populations in southern Xinjiang, China, which provide promising germplasm for further breeding and theoretical basis for biodiversity conservation and utilization for apricot population in this area.展开更多
Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Bat...Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Batsch cv. Feicheng) is described. The pMT18 clone is 1188 bp in length, with an open reading frame of 393 amino acids. The homology and phylogenetic analyses indicate a remarkable similarity between peach PG and other ripening related PG. And seven consensus sequences have revealed in peach PG compared to the PG from other plants. However, the profound divergence with other PG and the unique structure features suggest that peach PG probably belongs to a new evolutionary class. In RT PCR analysis, pMT18 related RNA was undetectable in leaves, and was much abundant in ripe fruits. The ripening specific expression pattern of this cDNA will be useful in investigating the roles of PG in fruit ripening and developing a transgenic peach with the improved post harvesting quality in the future.展开更多
A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot (Prunus armeniaca) were buried at the soil depths of 0-cm,...A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot (Prunus armeniaca) were buried at the soil depths of 0-cm, 4-cm, 8-cm, and 12-cm, respectively, to simulate the seed hoarding behavior of rodents in the field. The results revealed that the rates of seed germination and established seedlings from buried seeds were both the highest in 4-cm burial depth group, and then decreased with increasing soil depth. The number of rotten seeds increased in deeper burial depth. It is unfavourable for seed germination at 0-cm burial depth (i.e., seeds were laid on soil surface). There was insignificant effect of burial depth on growth of established seedlings. The results from this study indicated that proper burial depth in soil would be helpful for the seed germination and seedling growth. The seedlings derived from buried seeds at shallower depth (4 cm) in this research have advantage in their early development.展开更多
[Objective] The aim of this study was to explore the daily change of photosyntheticratefor Prunus domestica ×armeniacain different growing seasons. The study can provide theoretical basis for arid area high yield...[Objective] The aim of this study was to explore the daily change of photosyntheticratefor Prunus domestica ×armeniacain different growing seasons. The study can provide theoretical basis for arid area high yield and quality cultivation.[Method] The photosynthetic physiological properties of leaves of different types of Prunus domestica × armeniaca were measured by the Li-6400 portable photosynthesis system indifferent seasons. By this method could analysis of photosyntheticcharacteristicsfor different types of Prunus domestica×armeniaca in different seasons.[Result] Daily change of photosyntheticrate(Pn) for Prunus domestica×armeniaca in differentseasons showed a "double-peak" curve. The peak values were at 10:00 and16:00. The Pn of ‘Fengweihuanghou', ‘Konglongdan', ‘Weihou', ‘Weiwang' and‘Weidi' reached the maximum in July, theywere 13.75, 14.76, 12.96, 13.3, and 11.9μmol/(m^2·s), respectively. The Pn of Prunus domestica×armeniaca reached minimumin August, they were 9.78, 10.71, 12.02, 10.43 μmol/(m^2·s). The Pn overall average of ‘Konglongdan' was highest,it reached 12.65 μmol/(m^2·s).The Pn overall average of ‘Weiwang' was lowest, it reached 11.31μmol/(m^2·s). There were extremely significant positive correlation between the Pn and Gs(P0.01). [Conclusion] Daily change of photosyntheticrate for Prunus domestica ×armeniaca in differentseasons showed a "double-peak" curve, showing significant phenomenon of "midday depression".The photosynthesis intensity of Prunus domestica ×armeniaca was strongest in July, and the photosynthesis intensity was weakest in August. ‘Konglongdan'showed the strongest photosynthesis capacity, ‘Weihou' and ‘Weiwang', followed.There were highest correlation between the Pn and stoma conductance(Gs).展开更多
Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120...Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.展开更多
One-year-old seedlings of Amur maple (Acer ginnala Maxim), Ussurian pear (Pyrus ussuriensis Maxim) and David peach (Prunus davidiana Carr) were planted in pots in greenhouse and treated with four different soil moistu...One-year-old seedlings of Amur maple (Acer ginnala Maxim), Ussurian pear (Pyrus ussuriensis Maxim) and David peach (Prunus davidiana Carr) were planted in pots in greenhouse and treated with four different soil moisture contents (75.0%, 61.1%, 46.4% and 35.4%). The results showed that net photosynthesis rate (NPR), transpiration rate (TR) and stomatal conductance (Sc) of seedlings of the three species decreased with the decease of soil moisture content, and Amur maple seedlings had the greatest change in those physiological indices, followed by Ussurian pear, David peach. Amur maple and Ussurian pear seedlings also presented a decrease tendency in water use efficiency (WUE) under lower soil moisture content, whereas this was reversed for David peach. Under water stress the biomass allocation to seedling root had a significant increase for all the experimental species. As to root/shoot ratio, Amur maple seedlings had the biggest increase, while David peach had the smallest increase. The leaf plasticity of Amur maple seedlings was greater, the leaf size and total leaf area decreased significantly as the stress was intensified. No significant change of leaf size and total leaf area was found in seedlings of Ussurian pear and David peach. It was concluded that Amur maple was more tolerant to soil moisture stress in comparison with David peach and Ussurian pear.展开更多
基金Supported by Demonstration Project of Forestry Extension by Central Government(2022ZYTG002).
文摘This paper delves into and assesses the differences in biological traits and fruit nutritional composition of 3 varieties of Prunus humilis introduced from 2 different regions.Results showed that(i)the contents of protein,total sugar,and calcium of No.5 variety were higher those of the other 2 varieties,which was recognized as the most suitable variety for fresh fruit;(ii)the comparison of leaf areas index and fruit shape index of 3 varieties shows that the fruit shape index and individual fruit weight of the introduced varieties were higher than those of the control,the diameter of individual fruit of the introduced varieties higher than that of the control indicated that they had a higher fruit hardness,more dry matter accumulation and better fruit quality,and that the introduction region was more suitable for scale-up of P.humilis;(iii)there was a positive correlation with the number of fruiting branches and there was a significant negative correlation with individual fruit weight,while the leaf area had a significant negative correlation with the number of fruiting branches.The introduced varieties can develop normally in the 2 testing areas,notably,No.5 variety performed better than No.4 and No.6.Improving management is the prerequisite for maintaining proper number of fruiting branches and high yield.
文摘The stem barks of Prunus africana are used in the treatment of the benign prostate. Cameroon is one of the important exporters of the barks. Despite the important measures adopted in Cameroon for sustaining its harvesting, some many chalenges still remain. The objective of this work is to refine the forest management parameters in relation to P. africana in the regions of Adamaoua and the South-West by developing a volume rate which makes it possible to estimate the production for a new stem. The work took place in two phases: in the South-West in 2010 and in Adamaoua in 2011. Data collection used the semi-direct method, while the cubing equation was deduced by the multiple linear regression method. Two models for volume estimation and three models for mass prediction were developed. The predictive parameters retained are diameter, height of the bole and thickness of the bark. Results show that the average mass of the dry bark for a given P. africana tree species is 27.55 ± 14.44 kg and this varies according to the site. The strong adjusted coefficient of determination (adjusted R2) observed illustrates the reliability of the proposed models. These models provide a reliable tool that can be adopted as a standard in Cameroon for P. africana.
基金This work was supported by the National Natural Science Foundation (No. 30471196)the Special Program for Doctorial Site of Universities (No. 200404344011).
文摘Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous Region of China. The purpose of this study was to determine the genetic structure and genotypic diversity among the different eco-geographical populations. Based on the results from this study, 8 pairs of fluorescent-AFLP primers showed clear electrophoregram and high polymorphism amongst the 64 pairs of EcoR Ⅰ/Mse Ⅰ (Mse Ⅰ - a FAM fluorescent marked primer) primers screened. There was a significant polymorphic difference for the same primer pair in different populations and for the same population with different primer pairs. The percentage of polymorphic loci (P) at species level was higher than Kuche, Hetian, Kashi population levels, respectively. The Nei's gene diversity index (H) and Shannon's information index (I) at species level were higher than those of Kuche, Hetian, and Kashi at population level, respectively. H and I of Kuche population were the highest amongst the three populations. Apricot population genetic diversity was found mainly within the population, Genetic differentiation coefficient between populations (GST) was 0.0882. Gene flow Nm between the populations was 5.1689. Population genetic identity was between 0.9772-0.9811 and genetic distance was between 0.0191-0.0232. These results further indicated that the similarity between populations was higher and the genetic distance between populations was smaller. The UPGMA cluster analysis indicates that the geographical populations at Kuche, Kashi, Hetian were relatively independent Mendelian populations. Concurrently, there was also partial gene exchange between the populations. All the evidences indicated that the genetic diversity in Kuche population was the highest, suggesting that it could be a transition population from wild apricot to cultivated apricot. There were abundant genetic diversities in apricot cultivar populations in southern Xinjiang, China, which provide promising germplasm for further breeding and theoretical basis for biodiversity conservation and utilization for apricot population in this area.
文摘Polygalacturonase (PG,EC3.2.1.15) is the key cell wall hydrolase in fruit ripening. The identification and characterization of a full length cDNA (pMT18) encoding for PG from Feicheng peach (Prunus persica (L.) Batsch cv. Feicheng) is described. The pMT18 clone is 1188 bp in length, with an open reading frame of 393 amino acids. The homology and phylogenetic analyses indicate a remarkable similarity between peach PG and other ripening related PG. And seven consensus sequences have revealed in peach PG compared to the PG from other plants. However, the profound divergence with other PG and the unique structure features suggest that peach PG probably belongs to a new evolutionary class. In RT PCR analysis, pMT18 related RNA was undetectable in leaves, and was much abundant in ripe fruits. The ripening specific expression pattern of this cDNA will be useful in investigating the roles of PG in fruit ripening and developing a transgenic peach with the improved post harvesting quality in the future.
基金supported by National Basic Research Program of China (No. 2007CB109106)by the Zoology Key Subject Fund of Henan Province
文摘A semi-greenhouse study was conducted to understand the effects of soil burial depth on seed germination and seedling development. The seeds of wild apricot (Prunus armeniaca) were buried at the soil depths of 0-cm, 4-cm, 8-cm, and 12-cm, respectively, to simulate the seed hoarding behavior of rodents in the field. The results revealed that the rates of seed germination and established seedlings from buried seeds were both the highest in 4-cm burial depth group, and then decreased with increasing soil depth. The number of rotten seeds increased in deeper burial depth. It is unfavourable for seed germination at 0-cm burial depth (i.e., seeds were laid on soil surface). There was insignificant effect of burial depth on growth of established seedlings. The results from this study indicated that proper burial depth in soil would be helpful for the seed germination and seedling growth. The seedlings derived from buried seeds at shallower depth (4 cm) in this research have advantage in their early development.
基金Supported by High-quality and High-efficient Cultivation Technology Demonstration and Promotion of Apricot and Plum(ZYLYKJTG2015020)~~
文摘[Objective] The aim of this study was to explore the daily change of photosyntheticratefor Prunus domestica ×armeniacain different growing seasons. The study can provide theoretical basis for arid area high yield and quality cultivation.[Method] The photosynthetic physiological properties of leaves of different types of Prunus domestica × armeniaca were measured by the Li-6400 portable photosynthesis system indifferent seasons. By this method could analysis of photosyntheticcharacteristicsfor different types of Prunus domestica×armeniaca in different seasons.[Result] Daily change of photosyntheticrate(Pn) for Prunus domestica×armeniaca in differentseasons showed a "double-peak" curve. The peak values were at 10:00 and16:00. The Pn of ‘Fengweihuanghou', ‘Konglongdan', ‘Weihou', ‘Weiwang' and‘Weidi' reached the maximum in July, theywere 13.75, 14.76, 12.96, 13.3, and 11.9μmol/(m^2·s), respectively. The Pn of Prunus domestica×armeniaca reached minimumin August, they were 9.78, 10.71, 12.02, 10.43 μmol/(m^2·s). The Pn overall average of ‘Konglongdan' was highest,it reached 12.65 μmol/(m^2·s).The Pn overall average of ‘Weiwang' was lowest, it reached 11.31μmol/(m^2·s). There were extremely significant positive correlation between the Pn and Gs(P0.01). [Conclusion] Daily change of photosyntheticrate for Prunus domestica ×armeniaca in differentseasons showed a "double-peak" curve, showing significant phenomenon of "midday depression".The photosynthesis intensity of Prunus domestica ×armeniaca was strongest in July, and the photosynthesis intensity was weakest in August. ‘Konglongdan'showed the strongest photosynthesis capacity, ‘Weihou' and ‘Weiwang', followed.There were highest correlation between the Pn and stoma conductance(Gs).
基金Supported by Science and Technology Planning Project of Guang-dong Province (2007A020300002-6)~~
文摘Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.
基金This study was supported by Science and Technology Program of Heilongjiang Province (GC01KB213), and the Quick Response of Basic Research Supporting Program (2001CCB00600)
文摘One-year-old seedlings of Amur maple (Acer ginnala Maxim), Ussurian pear (Pyrus ussuriensis Maxim) and David peach (Prunus davidiana Carr) were planted in pots in greenhouse and treated with four different soil moisture contents (75.0%, 61.1%, 46.4% and 35.4%). The results showed that net photosynthesis rate (NPR), transpiration rate (TR) and stomatal conductance (Sc) of seedlings of the three species decreased with the decease of soil moisture content, and Amur maple seedlings had the greatest change in those physiological indices, followed by Ussurian pear, David peach. Amur maple and Ussurian pear seedlings also presented a decrease tendency in water use efficiency (WUE) under lower soil moisture content, whereas this was reversed for David peach. Under water stress the biomass allocation to seedling root had a significant increase for all the experimental species. As to root/shoot ratio, Amur maple seedlings had the biggest increase, while David peach had the smallest increase. The leaf plasticity of Amur maple seedlings was greater, the leaf size and total leaf area decreased significantly as the stress was intensified. No significant change of leaf size and total leaf area was found in seedlings of Ussurian pear and David peach. It was concluded that Amur maple was more tolerant to soil moisture stress in comparison with David peach and Ussurian pear.