Creating New Germplasm by Distant Hybridization in Stone Fruits:Ⅱ-Embryo Rescue and Hybrid Identification Between Plum and Apricot

Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.

九阡李果实发育期主要有机酸及氨基酸含量变化

以九阡李(Prunus salicina Lindl.)果实为试材,利用HPLC及LC-MS对九阡李果实发育过程中有机酸及氨基酸组分与含量变化进行分析。结果表明,九阡李果实中含有5种有机酸及22种氨基酸,有机酸以奎宁酸、琥珀酸为主,分别占总有机酸的64.35%、33.16%,氨基酸以天冬酰胺及脯氨酸为主,分别占总氨基酸的33.69%、20.82%。在九阡李果实发育过程中,奎宁酸含量变化趋势为先缓慢降低再急剧升高再降低,琥珀酸含量呈先缓慢降低再缓慢升高再缓慢降低的变化趋势,且二者都在第二次膨果期达到峰值,天冬酰胺含量呈先急剧升高再急剧降低接着再缓慢降低的变化趋势,在发育前期含量达到峰值,而脯氨酸含量变化趋势为先急剧上升后急剧下降再缓慢升高,在成熟期达到峰值。