Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enz...Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub strates.The biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.展开更多
文摘Glutamate dehydrogenase was purifiedfrom thecrudeextract of Pseudomonas pseudoal caligenes.Theenzyme had a molecular weight of 290 000 and was composed of six subunits with identical molecular weightof47 000 . The enzyme was highly specific for NADP(H) and the sub strates.The biochemicalpropertiessuch askineticparametersand heatstability werealsoexamined. The purified GDHshowed considerableloss ofactivity uponfreezing.