Bacillus subtilis （B. subtilis） and Pseudomonas fluorescens （P. fluorescens） are two of the most important plant growth promoting rhizobacteria （PGPR） in agriculture. An in situ trial was conducted on greenhouse...Bacillus subtilis （B. subtilis） and Pseudomonas fluorescens （P. fluorescens） are two of the most important plant growth promoting rhizobacteria （PGPR） in agriculture. An in situ trial was conducted on greenhouse tomato （Lycopersicum esculentum Mill.） to examine the effect of two bacterial strains, Bacillus subtilis （CGMCC 1.3343） and Pseudomonas fluorescens （CGMCC 1.1802）, on tomato growth, gray mold disease control, catabolic and genetic microbial features of indigenous rhizosphere bacteria under lownitrogen conditions. A commercial inoculant （ETS） was also tested as a comparison. Both B. subtilis and P. fluorescens promoted growth and biomass of seedlings, while only B. subtilis was efficient in reducing gray mold incidence in greenhouse tomato. The two bacterial strains could colonization in tomato rhizosphere soil at the end of experiment （10 days after the last inoculation）. Different AWCD trends and DGGE patterns were got in different bacterial treatments; however, analyses of microbial diversities showed that indigenous soil microbes did not seem to have significant differences at either the catabolic or genetic level among treatments. ETS, as a commercial microbial agent, promoted plant growth and gave a higher microbial diversity in rhizosphere soil.展开更多
The effluents of textile dyes are highly colored, and disposal of the wastes into receiving waters cause damage to the environment, therefore dyes removal is of great importance. In this research, removal of Orange II...The effluents of textile dyes are highly colored, and disposal of the wastes into receiving waters cause damage to the environment, therefore dyes removal is of great importance. In this research, removal of Orange II, Remazol Blue, Methyl red, Malachite green and Safranin dyes by Pseudomonas fluorescens was studied in batch system as function of temperature, pH and initial dye concentration. The rate of removal dyes was studied by using pectrophotometer. The optimum value was determined as pH 7 for all dyes tested about 87.8-72.7%, and optimum temperature for removal of all tested dyes was 30℃ about 86.6-60.8%. Higher removal of dyes observed at 25 mL for all tested dyes about 91.9-72,7%. In general, the increase in dye concentration inhibited the growth of bacteria. Pseudomonasfluorescens showed higher removal of Orange II among the dyes tested.展开更多
Crop disease is an important area which needs attention since most of the hazardous inputs added into the agricultural system are in the form of plant protection chemicals. Production of the crop is, however, constrai...Crop disease is an important area which needs attention since most of the hazardous inputs added into the agricultural system are in the form of plant protection chemicals. Production of the crop is, however, constrained by several disease infections including fungal diseases. The present study focused to isolate, Pseudomonasfluorescens possess a variety of promising properties which make it a better biocontrol agent. Two Pseudomonas fluorescens isolates from rhizospheric soil of faba bean were evaluated for their antagonistic activity against Botrytisfabae that is known to attack faba bean crops. Bio-primed faba bean seed with P f 9 and P f 10 （Pseudomonasfluorescens isolates 9 and Pseudomonasfluorescens isolates 10） for pathogencity test in green house was indicated an initiate positive result respectively. Two isolates of P f 9 and P f 10 reduced both disease severity and incidence. So it could be concluded that the used P f9 and P f 10 could resist the detrimental effects of Botrytisfabae on the plant growth and yield that will be used as biocontrol in the farm after field test.展开更多
基金Supported by the National High-tech Research and Development Program of China(2013AA102903)
文摘Bacillus subtilis （B. subtilis） and Pseudomonas fluorescens （P. fluorescens） are two of the most important plant growth promoting rhizobacteria （PGPR） in agriculture. An in situ trial was conducted on greenhouse tomato （Lycopersicum esculentum Mill.） to examine the effect of two bacterial strains, Bacillus subtilis （CGMCC 1.3343） and Pseudomonas fluorescens （CGMCC 1.1802）, on tomato growth, gray mold disease control, catabolic and genetic microbial features of indigenous rhizosphere bacteria under lownitrogen conditions. A commercial inoculant （ETS） was also tested as a comparison. Both B. subtilis and P. fluorescens promoted growth and biomass of seedlings, while only B. subtilis was efficient in reducing gray mold incidence in greenhouse tomato. The two bacterial strains could colonization in tomato rhizosphere soil at the end of experiment （10 days after the last inoculation）. Different AWCD trends and DGGE patterns were got in different bacterial treatments; however, analyses of microbial diversities showed that indigenous soil microbes did not seem to have significant differences at either the catabolic or genetic level among treatments. ETS, as a commercial microbial agent, promoted plant growth and gave a higher microbial diversity in rhizosphere soil.
文摘The effluents of textile dyes are highly colored, and disposal of the wastes into receiving waters cause damage to the environment, therefore dyes removal is of great importance. In this research, removal of Orange II, Remazol Blue, Methyl red, Malachite green and Safranin dyes by Pseudomonas fluorescens was studied in batch system as function of temperature, pH and initial dye concentration. The rate of removal dyes was studied by using pectrophotometer. The optimum value was determined as pH 7 for all dyes tested about 87.8-72.7%, and optimum temperature for removal of all tested dyes was 30℃ about 86.6-60.8%. Higher removal of dyes observed at 25 mL for all tested dyes about 91.9-72,7%. In general, the increase in dye concentration inhibited the growth of bacteria. Pseudomonasfluorescens showed higher removal of Orange II among the dyes tested.
文摘Crop disease is an important area which needs attention since most of the hazardous inputs added into the agricultural system are in the form of plant protection chemicals. Production of the crop is, however, constrained by several disease infections including fungal diseases. The present study focused to isolate, Pseudomonasfluorescens possess a variety of promising properties which make it a better biocontrol agent. Two Pseudomonas fluorescens isolates from rhizospheric soil of faba bean were evaluated for their antagonistic activity against Botrytisfabae that is known to attack faba bean crops. Bio-primed faba bean seed with P f 9 and P f 10 （Pseudomonasfluorescens isolates 9 and Pseudomonasfluorescens isolates 10） for pathogencity test in green house was indicated an initiate positive result respectively. Two isolates of P f 9 and P f 10 reduced both disease severity and incidence. So it could be concluded that the used P f9 and P f 10 could resist the detrimental effects of Botrytisfabae on the plant growth and yield that will be used as biocontrol in the farm after field test.
