The genetic diversity of both wild and reared Pseudosciaena crocea (Richardson) collected from Guan Jing Yang in Ningde, China in May 1999 was investigated by random amplified polymorphic DNA (RAPD) in the present stu...The genetic diversity of both wild and reared Pseudosciaena crocea (Richardson) collected from Guan Jing Yang in Ningde, China in May 1999 was investigated by random amplified polymorphic DNA (RAPD) in the present study. The polymorphism and mean difference of the wild population as revealed by RAPD were 18.9% and 0.0960 respectively, and those of the reared stocks were relatively lower, with 16.7% in polymorphism and 0.0747 in mean difference. The genetic distance between the two stocks was 0.0041. From the comprehensive investigation, the main reasons for the loss of genetic diversity were probably overfishing, small number of parents as broodstocks and the debatable artificial ranching. Results from this study also showed that the large yellow croaker populations distributed along Fujian coastal waters including Guan Jing Yang still potentially wide genetic variability. It is suggested that genetic management and prevention should be scientifically conducted in order to maintain and improve the genetic diversity of the P. crocea population .展开更多
Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI database.Primers for 48 ESTSSR loci were designed and ...Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI database.Primers for 48 ESTSSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China.Sixteen of the loci were polymorphic,which were amplified with 3 to 11 alleles per locus and the mean of 6.13.The observed and expected heterozygosity per locus ranged from 0.091 to 0.844 （mean 0.544） and from 0.118 to 0.892 （mean 0.644）,respectively.Polymorphic information content （PIC） ranged from 0.115 to 0.866 （mean 0.593）.The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on P.polyactis,C.niveatus,C.lucidus,A.argentatus and J.belengeri revealed that 14,12,11,7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these species.Overall,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.展开更多
文摘The genetic diversity of both wild and reared Pseudosciaena crocea (Richardson) collected from Guan Jing Yang in Ningde, China in May 1999 was investigated by random amplified polymorphic DNA (RAPD) in the present study. The polymorphism and mean difference of the wild population as revealed by RAPD were 18.9% and 0.0960 respectively, and those of the reared stocks were relatively lower, with 16.7% in polymorphism and 0.0747 in mean difference. The genetic distance between the two stocks was 0.0041. From the comprehensive investigation, the main reasons for the loss of genetic diversity were probably overfishing, small number of parents as broodstocks and the debatable artificial ranching. Results from this study also showed that the large yellow croaker populations distributed along Fujian coastal waters including Guan Jing Yang still potentially wide genetic variability. It is suggested that genetic management and prevention should be scientifically conducted in order to maintain and improve the genetic diversity of the P. crocea population .
基金The National High Technology Research and Development Program of China (863 program) under contract Nos 2006AA10A405 and 2006AA09Z418the National Natural Science Foundation of China under contract Nos 30771663 and 30471329the Foundation for Innovation Research Team of Jimei University,China under contract No.2006A001
文摘Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI database.Primers for 48 ESTSSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China.Sixteen of the loci were polymorphic,which were amplified with 3 to 11 alleles per locus and the mean of 6.13.The observed and expected heterozygosity per locus ranged from 0.091 to 0.844 （mean 0.544） and from 0.118 to 0.892 （mean 0.644）,respectively.Polymorphic information content （PIC） ranged from 0.115 to 0.866 （mean 0.593）.The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on P.polyactis,C.niveatus,C.lucidus,A.argentatus and J.belengeri revealed that 14,12,11,7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these species.Overall,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.
