Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from t...Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.展开更多
AIM To isolate the chemical components from extracted crude of Psoralea corylifolia L. METHODS Applicate the function of molecular recognition in supramolecular chemistry, use 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 dio...AIM To isolate the chemical components from extracted crude of Psoralea corylifolia L. METHODS Applicate the function of molecular recognition in supramolecular chemistry, use 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol as the host molecule and chemical components in extracted crude of Psoralea corylifolia L. as guest molecules, which represent different from either in sort, quantity and sites of function groups or in structural topological character, 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol could selectively form inclusion compound with component endowed with interactional complementarity and isolate as crystalline from the extracted crude of Psoralea corylifolia L. RESULTS The isopsoralen as guest molecule is selectively included by 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol in inclusion crystal and removed by acetone from the inclusion compound and total yield is 0 18%. Isopsoralen is determined by UV, IR, 1HNMR and MS and its inclusion compound is determined by means of IR, powder XRD and single crystal XRD. The isolation effect is analyzed by GC/MS. CONCLUSION The method is simple and selective for isolation isopsoralen from extracted crude of Psoralea corylifolia L.展开更多
文摘Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.
基金supported by the National Natural Science Foundation of China (81320108029)the National “Major Scientific and Technological Special Project for Significant New Drugs” project (2015ZX09501004-002-004)Specific Fund for Public Interest Research of Traditional Chinese Medicine,Ministry of Finance (201507004-002)。
文摘AIM To isolate the chemical components from extracted crude of Psoralea corylifolia L. METHODS Applicate the function of molecular recognition in supramolecular chemistry, use 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol as the host molecule and chemical components in extracted crude of Psoralea corylifolia L. as guest molecules, which represent different from either in sort, quantity and sites of function groups or in structural topological character, 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol could selectively form inclusion compound with component endowed with interactional complementarity and isolate as crystalline from the extracted crude of Psoralea corylifolia L. RESULTS The isopsoralen as guest molecule is selectively included by 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol in inclusion crystal and removed by acetone from the inclusion compound and total yield is 0 18%. Isopsoralen is determined by UV, IR, 1HNMR and MS and its inclusion compound is determined by means of IR, powder XRD and single crystal XRD. The isolation effect is analyzed by GC/MS. CONCLUSION The method is simple and selective for isolation isopsoralen from extracted crude of Psoralea corylifolia L.
