Background and objective Heparanase has been thought to be a good molecular marker of tumor,and the heparanase expression level was correlated closely with tumor metastasis. In this study,we investigate the effects of...Background and objective Heparanase has been thought to be a good molecular marker of tumor,and the heparanase expression level was correlated closely with tumor metastasis. In this study,we investigate the effects of heparanase on angiogenesis and lymphangiogenesis of lung cancer and the relationship between heparanase expression and vascular endothelial growth factor (VEGF),vascular endothelial growth factor-C (VEGF-C). Methods Immunohistochemistry was used to detect the expression of heparanase,VEGF,VEGF-C protein and microvascular density (MVD),lymphatic vessel density (LVD) in 115 cases of non-small cell lung cancer (NSCLC) and 45 cases of adjacent normal tissue samples. Results Our results showed that heparanase expression was significantly increased in 91 (79.13%) of the 115 cases and correlated with lymph node metastasis (node positive rate 87.0%; node negative rate 36.8%; P=0.003). Heparanase positive expression cases have significantly higher concentration of microvascular density (MVD) and lymphatic vessel density (LVD) as compared with heparanase negative expression cases (P<0.01,P<0.01,respectively),heparanase expression was significantly correlated with VEGF,VEGF-C expression in NSCLC. Conclusion Heparanase overexpression was associated with angiogenesis and lymphangiogenesis of lung cancer,targeting of heparanase may represent a significant therapeutic potential for lung cancer.展开更多
目的观察微波针刺配合生血润肤饮治疗寻常型银屑病的临床疗效。方法将100例寻常型银屑病患者随机分为对照组和观察组,每组50例。对照组采用口服生血润肤饮加减治疗,观察组在对照组基础上加用微波针刺治疗。观察两组治疗前后各项中医症候...目的观察微波针刺配合生血润肤饮治疗寻常型银屑病的临床疗效。方法将100例寻常型银屑病患者随机分为对照组和观察组,每组50例。对照组采用口服生血润肤饮加减治疗,观察组在对照组基础上加用微波针刺治疗。观察两组治疗前后各项中医症候(红斑、鳞屑、浸润增厚及皮损面积)积分、银屑病面积和严重程度指数(psoriasis area and severity index,PASI)评分、血清生物标志物[血管生成素2(angiopoietin 2,ANGPT2)、血管内皮生长因子受体3(vascular endothelial growth factor receptor-3,VEGFR-3)]水平及免疫功能各项指标(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))的变化情况,并比较两组临床疗效及不良反应发生率。结果观察组总有效率为96.0%,明显高于对照组的76.0%(P<0.05)。两组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均较同组治疗前显著下降,CD4^(+)、CD4^(+)/CD8^(+)均较同组治疗前显著上升,差异均具有统计学意义(P<0.05)。观察组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均明显低于对照组,CD4^(+)、CD4^(+)/CD8^(+)均明显高于对照组,差异均具有统计学意义(P<0.05)。对照组治疗期间不良反应发生率为4.0%,观察组为6.0%,两组比较差异无统计学意义(P>0.05)。结论微波针刺配合生血润肤饮是一种治疗寻常型银屑病的有效方法,能有效改善患者临床症状,降低血清ANGPT2及VEGFR-3水平,增强免疫功能,且安全可靠。展开更多
目的研究血管内皮生长因子(VEGF)基因单核苷酸多态性(SNP)与汉族人银屑病易感性的相关性。方法结合本课题组银屑病全基因组关联分析数据,在1103例银屑病患者和1104例正常对照中,应用Sequenom Mass Array质谱阵列技术对VEGF基因的SNP点rs...目的研究血管内皮生长因子(VEGF)基因单核苷酸多态性(SNP)与汉族人银屑病易感性的相关性。方法结合本课题组银屑病全基因组关联分析数据,在1103例银屑病患者和1104例正常对照中,应用Sequenom Mass Array质谱阵列技术对VEGF基因的SNP点rs2010963和rs9367178进行基因分型,用Plink1.03软件对数据资料进行统计分析。结果rs2010963(P=0.155)和rs9367178(P=0.65)位点等位基因频率在病例组和正常对照组之间的差异无统计学意义。对病例进行分层后分别与正常对照相比较,rs2010963和rs9367178的等位基因频率分布差异亦无统计学意义(P>0.05)。结论VEGF基因SNP点rs2010963和rs9367178可能与汉族人银屑病易感性不相关。展开更多
文摘Background and objective Heparanase has been thought to be a good molecular marker of tumor,and the heparanase expression level was correlated closely with tumor metastasis. In this study,we investigate the effects of heparanase on angiogenesis and lymphangiogenesis of lung cancer and the relationship between heparanase expression and vascular endothelial growth factor (VEGF),vascular endothelial growth factor-C (VEGF-C). Methods Immunohistochemistry was used to detect the expression of heparanase,VEGF,VEGF-C protein and microvascular density (MVD),lymphatic vessel density (LVD) in 115 cases of non-small cell lung cancer (NSCLC) and 45 cases of adjacent normal tissue samples. Results Our results showed that heparanase expression was significantly increased in 91 (79.13%) of the 115 cases and correlated with lymph node metastasis (node positive rate 87.0%; node negative rate 36.8%; P=0.003). Heparanase positive expression cases have significantly higher concentration of microvascular density (MVD) and lymphatic vessel density (LVD) as compared with heparanase negative expression cases (P<0.01,P<0.01,respectively),heparanase expression was significantly correlated with VEGF,VEGF-C expression in NSCLC. Conclusion Heparanase overexpression was associated with angiogenesis and lymphangiogenesis of lung cancer,targeting of heparanase may represent a significant therapeutic potential for lung cancer.
文摘目的观察微波针刺配合生血润肤饮治疗寻常型银屑病的临床疗效。方法将100例寻常型银屑病患者随机分为对照组和观察组,每组50例。对照组采用口服生血润肤饮加减治疗,观察组在对照组基础上加用微波针刺治疗。观察两组治疗前后各项中医症候(红斑、鳞屑、浸润增厚及皮损面积)积分、银屑病面积和严重程度指数(psoriasis area and severity index,PASI)评分、血清生物标志物[血管生成素2(angiopoietin 2,ANGPT2)、血管内皮生长因子受体3(vascular endothelial growth factor receptor-3,VEGFR-3)]水平及免疫功能各项指标(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))的变化情况,并比较两组临床疗效及不良反应发生率。结果观察组总有效率为96.0%,明显高于对照组的76.0%(P<0.05)。两组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均较同组治疗前显著下降,CD4^(+)、CD4^(+)/CD8^(+)均较同组治疗前显著上升,差异均具有统计学意义(P<0.05)。观察组治疗后各项中医症候积分、PASI评分、CD8^(+)及血清ANGPT2、VEGFR-3水平均明显低于对照组,CD4^(+)、CD4^(+)/CD8^(+)均明显高于对照组,差异均具有统计学意义(P<0.05)。对照组治疗期间不良反应发生率为4.0%,观察组为6.0%,两组比较差异无统计学意义(P>0.05)。结论微波针刺配合生血润肤饮是一种治疗寻常型银屑病的有效方法,能有效改善患者临床症状,降低血清ANGPT2及VEGFR-3水平,增强免疫功能,且安全可靠。
文摘目的研究血管内皮生长因子(VEGF)基因单核苷酸多态性(SNP)与汉族人银屑病易感性的相关性。方法结合本课题组银屑病全基因组关联分析数据,在1103例银屑病患者和1104例正常对照中,应用Sequenom Mass Array质谱阵列技术对VEGF基因的SNP点rs2010963和rs9367178进行基因分型,用Plink1.03软件对数据资料进行统计分析。结果rs2010963(P=0.155)和rs9367178(P=0.65)位点等位基因频率在病例组和正常对照组之间的差异无统计学意义。对病例进行分层后分别与正常对照相比较,rs2010963和rs9367178的等位基因频率分布差异亦无统计学意义(P>0.05)。结论VEGF基因SNP点rs2010963和rs9367178可能与汉族人银屑病易感性不相关。
文摘目的观察祛银汤治疗血热证型寻常性银屑病的临床疗效及其对外周血清中骨桥蛋白(osteopontin,OPN)和血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的影响。方法共入选24例血热证型寻常性银屑病患者,观察其治疗前、后皮损面积和严重性指数(psoriasis area and severity index,PA-SI)评分及中医症状评分的变化,并采用酶联免疫吸附试验(ELISA)检测患者治疗前、后外周血中OPN和VEGF的表达。结果祛银汤治疗8周后有效率为79.17%;治疗后PASI评分和各中医症状均较治疗前明显改善。治疗前银屑病患者外周血清OPN和VEGF表达明显升高,治疗后明显降低,差异均有统计学意义(P均<0.05),且OPN与PASI评分呈正相关关系(r=0.44,P<0.05)。结论血热证型寻常性银屑病患者OPN和VEGF异常表达,祛银汤可纠正外周血中异常的OPN和VEGF表达水平。
