对海南黎安港的企鹅珍珠贝养殖群体的生长进行了20个月的观察,每月测量其壳高(SH)、最大对角线长(MDL)、绞合线长(HL)和总重(TW)四个生长指标,并用模型y=y∞/(1+e-k(t-a)+d sin (2п/365.25)(t-b))分别拟合这四个指标的生长曲线。结果表...对海南黎安港的企鹅珍珠贝养殖群体的生长进行了20个月的观察,每月测量其壳高(SH)、最大对角线长(MDL)、绞合线长(HL)和总重(TW)四个生长指标,并用模型y=y∞/(1+e-k(t-a)+d sin (2п/365.25)(t-b))分别拟合这四个指标的生长曲线。结果表明,三个长度指标中MDL生长速度最快,其理论最大值约为112mm;HL的生长在初期快于SH,但到养殖实验结束时两者接近,理论最大值都在85mm左右;总重的增长在实验的前半段非常缓慢,但到后半段呈加速增长趋势,其理论最大值约为115g。企鹅珍珠贝的生长特征表现为初期长度增长迅速,经过大约一年左右,长度的增长减缓,而重量的增长开始加快,符合双壳贝一般的增长模式。研究也表明,在海南黎安港,降雨季节与快速生长季节存在明显的对应关系,暗示了降雨可能是导致生长出现季节性特征的诱因。展开更多
In response to various environmental factors in offshore South China Sea,a group of microstructures of the screw dislocation are assembled in multiphase screw dislocations on the surface of the nacreous layer of Pteri...In response to various environmental factors in offshore South China Sea,a group of microstructures of the screw dislocation are assembled in multiphase screw dislocations on the surface of the nacreous layer of Pteria penguin from the coastal waters of Hainan Island,China.The results of the transmission and scanning electronic microscope testing show that organic matter periodically secreted by epithelial histology of mantle of Pteria penguin pre-forms the original screw dislocation growth tem-plate in the form of film,along with its function to select,identify,and adhere to the amorphous calcium carbonate particles as the fine organic venations diffused in the epitaxial screw growth area.With the inducement and screw modulation of the or-ganic film and venations,the amorphous calcium carbonate particles gradually evolve into the pseudohexagonal self.aragonite microsheet with long-range order and screw dislocation structure by location-selecting tropism and screw dislocation growth.Numerous micron-scale aragonite microsheets join in the cluster's interactive movement and screw dislocation self-assembly of the organic film,stacking forward along the axis C spirally and forming the nacreous layer with screw dislocation structure.展开更多
Cadmium-metallothionein （Cd-MT） of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme...Cadmium-metallothionein （Cd-MT） of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme-linked immunosorbent assay （non-competitive ELISA） was established to detect shellfish Cd-MT. In this case, the minimum detectable concentration of P. penguin Cd-MT was （4.563±0.051） ng/mL. The linear range of detection was 9.75-2.0×104 ng/mL. Intra-assay relative standard deviation （RSD） was less than 11.6%, and inter-assay RSD less than 6.7%. The recovery rates ranged from 83.7% to 119.0%. The polyclonal antibody against Cd-MT of P. penguin can also be used to detect MT of other four types of shellfish. In the crude extracts from different organs of P. penguin, the MT concentrations determined by this method matched well with the concentrations of cadmium detected by the atomic absorption spectroscopy.展开更多
传统的形态鉴定主要依赖于分类者的经验和水平,容易产生分类错误。为提高物种鉴定的准确性,作者采用形态学与线粒体16S rRNA基因序列分析相结合的方式对广西北海涠洲岛采获的3种珍珠贝进行了鉴定。研究结果表明,基于形态学可将3种珍珠...传统的形态鉴定主要依赖于分类者的经验和水平,容易产生分类错误。为提高物种鉴定的准确性,作者采用形态学与线粒体16S rRNA基因序列分析相结合的方式对广西北海涠洲岛采获的3种珍珠贝进行了鉴定。研究结果表明,基于形态学可将3种珍珠贝分别鉴定为企鹅珍珠贝(Pteria penguin)、宽珍珠贝(P.loveni)和中国珍珠贝(P.chinensis),但基于16S r RNA基因序列,形态学鉴定为P.loveni的标本应为P.lata。P.loveni和P.lata是两个完全独立的种,并非为同物异名。珍珠贝属16S rRNA基因序列的种间遗传距离明显大于种内遗传距离,适宜作为该属种类鉴定的分子标记。在基于16S r RNA基因序列构建的系统树中,相同种类的不同个体均形成单系群,并获得很高的支持率。分布于中国沿海的珍珠贝属种类应包括P.lata,是否有P.loveni的分布尚需进一步证实。展开更多
文摘对海南黎安港的企鹅珍珠贝养殖群体的生长进行了20个月的观察,每月测量其壳高(SH)、最大对角线长(MDL)、绞合线长(HL)和总重(TW)四个生长指标,并用模型y=y∞/(1+e-k(t-a)+d sin (2п/365.25)(t-b))分别拟合这四个指标的生长曲线。结果表明,三个长度指标中MDL生长速度最快,其理论最大值约为112mm;HL的生长在初期快于SH,但到养殖实验结束时两者接近,理论最大值都在85mm左右;总重的增长在实验的前半段非常缓慢,但到后半段呈加速增长趋势,其理论最大值约为115g。企鹅珍珠贝的生长特征表现为初期长度增长迅速,经过大约一年左右,长度的增长减缓,而重量的增长开始加快,符合双壳贝一般的增长模式。研究也表明,在海南黎安港,降雨季节与快速生长季节存在明显的对应关系,暗示了降雨可能是导致生长出现季节性特征的诱因。
基金supported by National Basic Research Program of China (Grant No. 49772096)National Basic Research Program of Hubei,China (Grant No. 2005ABA005)
文摘In response to various environmental factors in offshore South China Sea,a group of microstructures of the screw dislocation are assembled in multiphase screw dislocations on the surface of the nacreous layer of Pteria penguin from the coastal waters of Hainan Island,China.The results of the transmission and scanning electronic microscope testing show that organic matter periodically secreted by epithelial histology of mantle of Pteria penguin pre-forms the original screw dislocation growth tem-plate in the form of film,along with its function to select,identify,and adhere to the amorphous calcium carbonate particles as the fine organic venations diffused in the epitaxial screw growth area.With the inducement and screw modulation of the or-ganic film and venations,the amorphous calcium carbonate particles gradually evolve into the pseudohexagonal self.aragonite microsheet with long-range order and screw dislocation structure by location-selecting tropism and screw dislocation growth.Numerous micron-scale aragonite microsheets join in the cluster's interactive movement and screw dislocation self-assembly of the organic film,stacking forward along the axis C spirally and forming the nacreous layer with screw dislocation structure.
基金Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-48-07B)Guangdong Provincial Agriculture Key Project (2010B020313005)
文摘Cadmium-metallothionein （Cd-MT） of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme-linked immunosorbent assay （non-competitive ELISA） was established to detect shellfish Cd-MT. In this case, the minimum detectable concentration of P. penguin Cd-MT was （4.563±0.051） ng/mL. The linear range of detection was 9.75-2.0×104 ng/mL. Intra-assay relative standard deviation （RSD） was less than 11.6%, and inter-assay RSD less than 6.7%. The recovery rates ranged from 83.7% to 119.0%. The polyclonal antibody against Cd-MT of P. penguin can also be used to detect MT of other four types of shellfish. In the crude extracts from different organs of P. penguin, the MT concentrations determined by this method matched well with the concentrations of cadmium detected by the atomic absorption spectroscopy.
文摘传统的形态鉴定主要依赖于分类者的经验和水平,容易产生分类错误。为提高物种鉴定的准确性,作者采用形态学与线粒体16S rRNA基因序列分析相结合的方式对广西北海涠洲岛采获的3种珍珠贝进行了鉴定。研究结果表明,基于形态学可将3种珍珠贝分别鉴定为企鹅珍珠贝(Pteria penguin)、宽珍珠贝(P.loveni)和中国珍珠贝(P.chinensis),但基于16S r RNA基因序列,形态学鉴定为P.loveni的标本应为P.lata。P.loveni和P.lata是两个完全独立的种,并非为同物异名。珍珠贝属16S rRNA基因序列的种间遗传距离明显大于种内遗传距离,适宜作为该属种类鉴定的分子标记。在基于16S r RNA基因序列构建的系统树中,相同种类的不同个体均形成单系群,并获得很高的支持率。分布于中国沿海的珍珠贝属种类应包括P.lata,是否有P.loveni的分布尚需进一步证实。
