[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with dif...[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.展开更多
In order to explore the effect of radiation breeding on new germplasm innovation of Pteroceltis tatarinowii,the dry seeds of P.tatarinowii from Lingyan Temple were used as test materials in this study,which were irrad...In order to explore the effect of radiation breeding on new germplasm innovation of Pteroceltis tatarinowii,the dry seeds of P.tatarinowii from Lingyan Temple were used as test materials in this study,which were irradiated by ^(60)Co-γ rays with different radiation doses.The results showed that the emergence rate decreased with the increase of radiation dose,the emergence rate of low dose below 200 Gy decreased slowly,and the emergence rate of high dose above 200 Gy decreased sharply;when the radiation dose ranged from 100 to 200 Gy,the mutagenic effect of P.tatarinowii seeds was the best;and radiation obviously affected the leaf color and leaf shape of seedlings.This study provides original materials for the cultivation of new P.tatarinowii varieties and the study of color-changing mechanism.展开更多
基金Supported by Agricultural Seed Breeding Program of Shandong Province in 2014([2014]96)
文摘[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.
基金Supported by"Innovation Driving and Leading Promotion of Seedling and Flower Industry in Mount Tai"of Industrial Upgrading Project of Science and Technology Park in Shandong Province(2019YQ012)"Collection,Preservation and Accurate Identification of Germplasm Resources of Precious Timber Tree Species"of Shandong Provincial Agricultural Elite Varieties Project(2019LZGC01804).
文摘In order to explore the effect of radiation breeding on new germplasm innovation of Pteroceltis tatarinowii,the dry seeds of P.tatarinowii from Lingyan Temple were used as test materials in this study,which were irradiated by ^(60)Co-γ rays with different radiation doses.The results showed that the emergence rate decreased with the increase of radiation dose,the emergence rate of low dose below 200 Gy decreased slowly,and the emergence rate of high dose above 200 Gy decreased sharply;when the radiation dose ranged from 100 to 200 Gy,the mutagenic effect of P.tatarinowii seeds was the best;and radiation obviously affected the leaf color and leaf shape of seedlings.This study provides original materials for the cultivation of new P.tatarinowii varieties and the study of color-changing mechanism.