[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with dif...[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.展开更多
In order to explore the effect of radiation breeding on new germplasm innovation of Pteroceltis tatarinowii,the dry seeds of P.tatarinowii from Lingyan Temple were used as test materials in this study,which were irrad...In order to explore the effect of radiation breeding on new germplasm innovation of Pteroceltis tatarinowii,the dry seeds of P.tatarinowii from Lingyan Temple were used as test materials in this study,which were irradiated by ^(60)Co-γ rays with different radiation doses.The results showed that the emergence rate decreased with the increase of radiation dose,the emergence rate of low dose below 200 Gy decreased slowly,and the emergence rate of high dose above 200 Gy decreased sharply;when the radiation dose ranged from 100 to 200 Gy,the mutagenic effect of P.tatarinowii seeds was the best;and radiation obviously affected the leaf color and leaf shape of seedlings.This study provides original materials for the cultivation of new P.tatarinowii varieties and the study of color-changing mechanism.展开更多
The bark of Pteroce/tis tatarinowii is a raw material for manufacturing XuanPaper. The effects of Ca^(2+) concentrations on the accumulation of mineral elements in the bark,leaf and root of Pteroceltis tatarinowii wer...The bark of Pteroce/tis tatarinowii is a raw material for manufacturing XuanPaper. The effects of Ca^(2+) concentrations on the accumulation of mineral elements in the bark,leaf and root of Pteroceltis tatarinowii were studied under controlled conditions. The types ofHoagland nutrient solution with three Ca^(2+) concentrations levels (200, 400 and 600 μg·g^(-1))and a control (without Ca^(2+)) were designed to culture Pteroceltis tatarinowii. After 6 months,contents of seven mineral elements including Ca, K, Mg, Mn, Zn, Cu and Na in the root, leaf and barkwere analyzed. The results indicated that Ca accumulations content in the root, leaf and bark hadpositively relation with Ca^(2+) concentrations (200, 400, 600 μg · g^(-1)), and the order of theCa content in the three components was root>leaf>bark. Ca content in the root treated with 600 μg·g^(-1) Ca^(2+) concentrations was 5.5 times as high as that of the control, and about 1.4 times ashigh as that of the root treated in 200 and 400 μg/g Ca^(2+) concentrations respectively. On thecontrary, K and Mg contents in the root, leaf and bark were negatively related to Ca^(2+)concentrations, especially in the bark, and their accumulation trend followed the order ofleaf>root>bark. K content in the bark treated with 600 μg ·g^(-1) Ca^(2+) concentrations was 39.3%of that of the control, and was 79.0% and 91.8% of that of the bark treated with 200 μg ·g^(-1)and 400 μg ·g^(-1) Ca^(2+) concentrations respectively; Mg content in the bark treated with 600μg ·g^(-1) Ca^(2+) concentrations was 23.4% of that of the control, and was 27.1% and 35.4% ofthat of the bark treated with 200 and 400 μg·g^(-1) Ca^(2+) concentrations respectively. Comparedwith the control, the general tendency of Mn, Zn and Cu content decreased with increasing of Ca^(2+)concentrations and their contents were in the order: root>leaf>bark. Based on the results of thisstudy, the experiment has been useful for providing academic bases in improving the bark quality ofPteroceltis tatarinowii on non-limestone soil.展开更多
The bark of Pteroceltis tatarinowii is a raw material for manufacturing Xuan Paper. The effects of Ca2+ concentrations on the accumulation of mineral elements in the bark, leaf and root of Pteroceltis tatarinowii were...The bark of Pteroceltis tatarinowii is a raw material for manufacturing Xuan Paper. The effects of Ca2+ concentrations on the accumulation of mineral elements in the bark, leaf and root of Pteroceltis tatarinowii were studied under controlled condi-tions. The types of Hoagland nutrient solution with three Ca2+ concentrations levels (200, 400 and 600 靏g-1) and a control (without Ca2+) were designed to culture Pteroceltis tatarinowii. After 6 months, contents of seven mineral elements including Ca, K, Mg, Mn, Zn, Cu and Na in the root, leaf and bark were analyzed. The results indicated that Ca accumulations content in the root, leaf and bark had positively relation with Ca2+ concentrations (200, 400, 600 靏g-1), and the order of the Ca content in the three components was root】leaf】bark. Ca content in the root treated with 600 靏g-1 Ca2+ concentrations was 5.5 times as high as that of the control, and about 1.4 times as high as that of the root treated in 200 and 400 靏/g Ca2+ concentrations respectively. On the contrary, K and Mg contents in the root, leaf and bark were negatively related to Ca2+ concentrations, especially in the bark, and their accumulation trend followed the order of leaf】root】bark. K content in the bark treated with 600 靏g-1 Ca2+ con-centrations was 39.3% of that of the control, and was 79.0% and 91.8% of that of the bark treated with 200 靏g-1 and 400靏g-1 Ca2+ concentrations respectively; Mg content in the bark treated with 600 靏g-1 Ca2+ concentrations was 23.4% of that of the control, and was 27.1% and 35.4% of that of the bark treated with 200 and 400 靏g-1 Ca2+ concentrations respectively. Com-pared with the control, the general tendency of Mn, Zn and Cu content decreased with increasing of Ca2+ concentrations and their contents were in the order: root】leaf】bark. Based on the results of this study, the experiment has been useful for providing academic bases in improving the bark quality of Pteroceltis tatarinowii on non-limestone soil.展开更多
Wild resources of Pteroceltis tatarinowii Maxim.were investigated and morphological characteristics of leaves,fruits and seeds were measured in 2005.In Lingyan Temple of Shandong,the trees grow in the temple and along...Wild resources of Pteroceltis tatarinowii Maxim.were investigated and morphological characteristics of leaves,fruits and seeds were measured in 2005.In Lingyan Temple of Shandong,the trees grow in the temple and along the roads.In addition to the two Pteroceltis tatarinowii,the average DBH and height of the other trees were 24.32 cm and 10.05 m respectively.The stands in Miaofeng Mountain distributed only in the valley of Dishuiyan with the average DBH of 16.12 cm and average height of 11.50 cm.Pteroceltis tatarinowii could naturally reproduce in wild habitat and only a few natural reproduced seedlings were found at Lingyan Temple.The results of variance analysis showed that there was significant difference of 9 characteristics among different individual trees of the same source.Except for width of fruit wing,8 morphological characteristics significantly differed among three sources.Significant difference exited in leaf length and leaf width among three sources.Although there was no significant difference in seed and nut features between two different sources in Beijing,those characteristics of Pteroceltis tatarinowii from Beijing source were obviously different with those from Shangdong.The seeds from Shandong were the smallest and lightest.There was distinctness in morphological quality characteristics from different trees.展开更多
基金Supported by Agricultural Seed Breeding Program of Shandong Province in 2014([2014]96)
文摘[Objective] This study was conducted to obtain tetraploid Pteroceltis tatar# nowii Maxim. with excellent ornamental traits. [Method] The stem apex growing points of Pteroceltis tatarinowii Maxim. were treated with different concentrations of colchicine solution for different hours to figure out a proper method and obtain poly- ploids. [Result] The most effective induction was obtained by treatment with 0.6%- 0.8% colchicine for 72 h with 34.2% mutation rate. Flow cytometry and chromosome observation of the stem apex growing point of P. tatarinowii Maxim. proved that the tetraploid plants were successfully obtained with chromosome number 2n=4x=36. [Conclusion] The result not only fills the blank of polyploid breeding of P. tatarinowii, but also provides an effective way to broaden the methods of cultivation of fast- growing, high-quality, disease-resilience, new varieties of Pteroceltis.
基金Supported by"Innovation Driving and Leading Promotion of Seedling and Flower Industry in Mount Tai"of Industrial Upgrading Project of Science and Technology Park in Shandong Province(2019YQ012)"Collection,Preservation and Accurate Identification of Germplasm Resources of Precious Timber Tree Species"of Shandong Provincial Agricultural Elite Varieties Project(2019LZGC01804).
文摘In order to explore the effect of radiation breeding on new germplasm innovation of Pteroceltis tatarinowii,the dry seeds of P.tatarinowii from Lingyan Temple were used as test materials in this study,which were irradiated by ^(60)Co-γ rays with different radiation doses.The results showed that the emergence rate decreased with the increase of radiation dose,the emergence rate of low dose below 200 Gy decreased slowly,and the emergence rate of high dose above 200 Gy decreased sharply;when the radiation dose ranged from 100 to 200 Gy,the mutagenic effect of P.tatarinowii seeds was the best;and radiation obviously affected the leaf color and leaf shape of seedlings.This study provides original materials for the cultivation of new P.tatarinowii varieties and the study of color-changing mechanism.
基金This paper is supported by National Natural Science Foundation of China (No. 39970608).
文摘The bark of Pteroce/tis tatarinowii is a raw material for manufacturing XuanPaper. The effects of Ca^(2+) concentrations on the accumulation of mineral elements in the bark,leaf and root of Pteroceltis tatarinowii were studied under controlled conditions. The types ofHoagland nutrient solution with three Ca^(2+) concentrations levels (200, 400 and 600 μg·g^(-1))and a control (without Ca^(2+)) were designed to culture Pteroceltis tatarinowii. After 6 months,contents of seven mineral elements including Ca, K, Mg, Mn, Zn, Cu and Na in the root, leaf and barkwere analyzed. The results indicated that Ca accumulations content in the root, leaf and bark hadpositively relation with Ca^(2+) concentrations (200, 400, 600 μg · g^(-1)), and the order of theCa content in the three components was root>leaf>bark. Ca content in the root treated with 600 μg·g^(-1) Ca^(2+) concentrations was 5.5 times as high as that of the control, and about 1.4 times ashigh as that of the root treated in 200 and 400 μg/g Ca^(2+) concentrations respectively. On thecontrary, K and Mg contents in the root, leaf and bark were negatively related to Ca^(2+)concentrations, especially in the bark, and their accumulation trend followed the order ofleaf>root>bark. K content in the bark treated with 600 μg ·g^(-1) Ca^(2+) concentrations was 39.3%of that of the control, and was 79.0% and 91.8% of that of the bark treated with 200 μg ·g^(-1)and 400 μg ·g^(-1) Ca^(2+) concentrations respectively; Mg content in the bark treated with 600μg ·g^(-1) Ca^(2+) concentrations was 23.4% of that of the control, and was 27.1% and 35.4% ofthat of the bark treated with 200 and 400 μg·g^(-1) Ca^(2+) concentrations respectively. Comparedwith the control, the general tendency of Mn, Zn and Cu content decreased with increasing of Ca^(2+)concentrations and their contents were in the order: root>leaf>bark. Based on the results of thisstudy, the experiment has been useful for providing academic bases in improving the bark quality ofPteroceltis tatarinowii on non-limestone soil.
基金This paper is supported by National Natural Science Foundation of China (No. 39970608).
文摘The bark of Pteroceltis tatarinowii is a raw material for manufacturing Xuan Paper. The effects of Ca2+ concentrations on the accumulation of mineral elements in the bark, leaf and root of Pteroceltis tatarinowii were studied under controlled condi-tions. The types of Hoagland nutrient solution with three Ca2+ concentrations levels (200, 400 and 600 靏g-1) and a control (without Ca2+) were designed to culture Pteroceltis tatarinowii. After 6 months, contents of seven mineral elements including Ca, K, Mg, Mn, Zn, Cu and Na in the root, leaf and bark were analyzed. The results indicated that Ca accumulations content in the root, leaf and bark had positively relation with Ca2+ concentrations (200, 400, 600 靏g-1), and the order of the Ca content in the three components was root】leaf】bark. Ca content in the root treated with 600 靏g-1 Ca2+ concentrations was 5.5 times as high as that of the control, and about 1.4 times as high as that of the root treated in 200 and 400 靏/g Ca2+ concentrations respectively. On the contrary, K and Mg contents in the root, leaf and bark were negatively related to Ca2+ concentrations, especially in the bark, and their accumulation trend followed the order of leaf】root】bark. K content in the bark treated with 600 靏g-1 Ca2+ con-centrations was 39.3% of that of the control, and was 79.0% and 91.8% of that of the bark treated with 200 靏g-1 and 400靏g-1 Ca2+ concentrations respectively; Mg content in the bark treated with 600 靏g-1 Ca2+ concentrations was 23.4% of that of the control, and was 27.1% and 35.4% of that of the bark treated with 200 and 400 靏g-1 Ca2+ concentrations respectively. Com-pared with the control, the general tendency of Mn, Zn and Cu content decreased with increasing of Ca2+ concentrations and their contents were in the order: root】leaf】bark. Based on the results of this study, the experiment has been useful for providing academic bases in improving the bark quality of Pteroceltis tatarinowii on non-limestone soil.
文摘为探究野生青檀根部真菌的群落组成及多样性,揭示青檀根际土壤真菌和根内生真菌的群落结构特征及两者之间的潜在联系,以安徽琅琊山野生青檀的根际土壤和根为研究对象,应用Illumina MiSeq高通量测序技术对根际土壤真菌和根内生真菌的18 S rRNA基因ITS1-ITS2区进行测序,并进行相关性分析。结果表明,青檀根际土壤真菌群落由13门43纲109目258科521属组成,青檀根内生真菌由12门39纲96目217科409属组成。青檀根际土壤真菌和根内生真菌群落主要由子囊菌门和担子菌门组成,分别占71.80%、12.99%和65.16%、21.66%;粪壳菌纲为两者的最大优势菌纲,分别占26.11%和49.20%;根际土壤真菌和根内生真菌以柔膜菌目和未分类的粪壳菌纲为第一优势菌目,分别占15.74%和31.00%;属水平上,根际土壤真菌和根内生真菌以Mycoarthris和未分类的粪壳菌纲为第一优势菌属,分别占14.64%和31.00%,两个真菌群落菌属组成的相似性程度较高(Jaccard相似性系数为0.68)。青檀根际土壤真菌群落的多样性(Shannon指数为4.42,Simpson指数为0.11)高于根内生真菌群落(Shannon指数为3.11,Simpson指数为0.17)。Chao1和ACE指数表明两个群落之间的丰度差异显著(P<0.05)。相似性分析(ANOSIM)表明,青檀根际土壤和根内不同生态环境下的真菌群落组成差异显著(r=0.85,P<0.05),且组间差异显著大于组内各样品之间差异。野生青檀根际土壤真菌和根内生真菌组成丰富,两个群落之间既存在相似性,又存在明显差异。
文摘Wild resources of Pteroceltis tatarinowii Maxim.were investigated and morphological characteristics of leaves,fruits and seeds were measured in 2005.In Lingyan Temple of Shandong,the trees grow in the temple and along the roads.In addition to the two Pteroceltis tatarinowii,the average DBH and height of the other trees were 24.32 cm and 10.05 m respectively.The stands in Miaofeng Mountain distributed only in the valley of Dishuiyan with the average DBH of 16.12 cm and average height of 11.50 cm.Pteroceltis tatarinowii could naturally reproduce in wild habitat and only a few natural reproduced seedlings were found at Lingyan Temple.The results of variance analysis showed that there was significant difference of 9 characteristics among different individual trees of the same source.Except for width of fruit wing,8 morphological characteristics significantly differed among three sources.Significant difference exited in leaf length and leaf width among three sources.Although there was no significant difference in seed and nut features between two different sources in Beijing,those characteristics of Pteroceltis tatarinowii from Beijing source were obviously different with those from Shangdong.The seeds from Shandong were the smallest and lightest.There was distinctness in morphological quality characteristics from different trees.
