Direct Correlation among Telomere Length, Cellular Aging, and Rejuvenation Effects of Honey Child Powder

Purpose: Telomere length (TL) is an indicator of age;however, hormonal influences complicate individual aging. It remains unclear whether TL shortening is a direct factor in both individual and cellular aging. Therefore, we examined the direct relationship between TL and cellular senescence at the cellular level. Methods: Telomerase activity, TL, and gene expression were measured in cultured human lung-, fetal-, and skin-derived fibroblasts, human skin keratinocytes, and telomerase reverse transcriptase (TERT) gene-immortalized cells using detection kits, Cawthon’s method, and reverse transcription-quantitative polymerase chain reaction, respectively. Novel substances that elongate telomeres were screened to confirm cell rejuvenation effects. Results: Long-term cell culture of TIG-1-20 normal human fibroblasts resulted in TL shortening, decreased division rate, and senescence progression, whereas in OUMS-36T-2 cells, TL elongation via TERT gene transfer increased the division rate, reduced endoplasmic reticulum stress, and upregulated genes associated with young individuals, indicating that cellular rejuvenation occurs via TL elongation. In addition, a honey child powder (HCP) extract was found through screening, and the HCP extract strongly suppressed the menin gene, resulting in increased telomerase activity and extended cell lifespan. Upon addition of the HCP extract to skin fibroblasts, gene expression of moisturizing components, including collagen, hyaluronic acid, and elastin, increased, and exhibited a rejuvenating effect with an increase in elastin amount. Conclusions: TL elongation or shortening is involved in cell proliferation rate and cellular aging, and TL elongation rejuvenates cells. In addition, HCP extract has a rejuvenating effect on cells and is expected to be a rejuvenating compound.

Evaluation of total protein,peroxidase,and nutrients measured by pXRF for the determination of tissue rejuvenation/reinvigoration of Eucalyptus microcorys

Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species,hampering clonal forestry programs.This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile,peroxidase activity,macro-and micronutrient contents,and adventitious rooting of mini cuttings.The analyses were performed on E.microcorys shoots which were successfully obtained by seminal and grafting propagation,micropropagation with epicormic shoots,and indirect organogenesis.Among four mature trees used in the propagation,tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration.Five individuals from each technique were randomly selected and transferred to a semi-hydroponic"channel"system.After four weeks in the seedbed,the total protein,peroxidase activity,nutrient content and rooting of the mini cuttings were evaluated.SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques,as revealed by two distinct bands.Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity,while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity.A portable X-ray fluorescence spectroscope(pXRF)identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation,and by indirect organogenesis.The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation(75%)followed by indirect organogenesis(35%).Based on principal component analysis,it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis was associated with phosphorous,sulphur,and potassium contents,which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants.Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages.

The Combined Effect of Botulinum Toxin Type A with a Biorevitalizing Treatment on Forehead Rejuvenation: A Case Series

Background: The forehead is particularly prone to repetitive facial expressions and hyperdynamic activity, resulting in deep wrinkles and a loss of elasticity and hydration. Although botulinum toxin type A (BoNTA) has been shown to reduce the appearance of wrinkles and allow patients to have a smoother and hence younger upper face, it cannot help to improve the other aspects that determine overall skin quality. Objective: This case series aims to evaluate the clinical efficacy of combining BoNTA injections with a biorevitalizing treatment (NCTF®135HA) on skin aging signs and quality using a split face approach. Patients and Methods: A total of eight patients from 30 to 55 years old were treated with botulinum toxin type A combined with NCTF®-135HA or alone. Results: At D60, clinical skin quality scoring showed that NCTF®135HA injection significantly reduced wrinkles and enhanced skin homogeneity, hydration, firmness, and radiance. The Global Aesthetic Improvement Scale (GAIS) by the evaluator at D60 reported mean scores of 2375 (much improved) on the side of the face treated with BoNTA + NCTF®-135HA compared to the side of the face without NCTF®135HA. According to patients, the NCTF®135HA injections resulted in a significantly better aesthetic improvement score on D60 (mean score of 2.5 compared to 0.125 without NCTF®135HA, p 0.001). Conclusions: Our findings show that combining a biorevitalizing treatment with a BoNTA injection can significantly improve the skin’s overall appearance and decrease the wrinkle score when compared to the BoNTA injections alone. The results suggest that the combinatory strategy is effective, safe, and associated with a high level of patient satisfaction.

A Non-Invasive Skin Treatment Combining LED with Pharmacologic and Ultrasonic Technologies for Facial Rejuvenation

Background: Non-invasive facial treatments have the ability to rejuvenate the facial profile when specific pharmacologic agents and modalities are prescribed and used in combination taking into consideration each patient’s unique skin type and condition. RATIONALE Epinova is a non-invasive skin treatment that combines the correct concentrations and combinations of topicals and modalities to elicit facial rejuvenation with no down-time or side effects. Purpose: This paper focuses on facial rejuvenation improvements combining the RATIONALE Essential Six skincare system (RATIONALE, Victoria, Australia) to protect and repair the skin with the RATIONALE Epinova facial treatment every 4-6 weeks—which uses non-invasive technologies and professional strength active ingredients to deliver visible changes to skin tone and texture. Methods: Subjects underwent a RATIONALE consultation, including taking a skin history and skin imaging, followed by a data analysis and diagnosis of skin condition and prescription of a customized RATIONALE treatement (Epinova), including appropriate pharmacologic agents and treatment with personalized photo/sono therapeutic devices. Results: Subjects reported increased skin hydration, tactile improvements, skin firmness and visible radiance following the RATIONALE Epinova treatment. Further investigations will be initiated to explore the potential for longer term improvements, including connenctive tissue deposition, reduction of erythema etc. Treatments should be performed every 4-6 weeks for patients under 40 and every 3-4 weeks for patients over 40, to support cell differentiation, migration and desquamation to achieve non-invasive facial rejuvenation. Conclusion: This study demonstrated that the synergy of pharmacologic, LED light therapy and ultrasonic technologies when prescribed and administered by a trained skin therapist, can lead to a visible improvement in the signs of facial ageing and photodamage, restoring the appearance of healthy, radiant skin. .

Bio-based rejuvenators in asphalt pavements:A comprehensive review and analytical study

The pressing demand for sustainable advancements in road infrastructure has catalyzed extensive research into environmentally conscious alternatives for the maintenance and restoration of asphalt concrete pavements.This paper offers a comprehensive review and analysis of bio-based rejuvenators as a promising avenue for enhancing the longevity and sustainability of asphalt.Through a multifaceted exploration,it delves into various aspects of this innovative approach.Providing a thorough overview of bio-based rejuvenators,the study highlights their renewable and environmentally friendly characteristics.It conducts an in-depth examination of a wide spectrum of bio-derived materials,including vegetable oils,waste-derived bio-products,and biopolymers,through a comprehensive survey.The paper evaluates how bio-based rejuvenators enhance aged asphalt binders and mixes,effectively mitigating the adverse impacts of aging.Furthermore,it investigates how these rejuvenators address environmental concerns by identifying compatibility issues,assessing long-term performance,and evaluating economic feasibility.Finally,the paper outlines potential advancements and research pathways aimed at optimizing the utilization of bio-based rejuvenators in asphalt concrete,thereby contributing to the sustainable evolution of road infrastructure.

基于检查点和Rejuvenation的软件可靠性建模分析

处于运行阶段的软件往往存在老化现象,即性能的逐渐下降或失效。为了避免软件老化造成的影响,人们提出了软件rejuvenation技术。这是一种预防性的软件容错策略,能有效的提高软件系统的可靠性和可用性。但由于rejuvenation操作过程的复杂性,它的成本也是不可忽略的。相对于rejuvenation来说,检查点是一种轻量级的软件容错策略,其成本远小于rejuvenation的成本。本文旨在结合rejuvenation和检查点技术,在rejuvenation周期间插入适当数目的检查点,以降低系统的宕机成本。文中给出了系统的petri网模型,并结合实例进行了分析。

基于Rejuvenation的程序执行时间优化算法

Rejuvenation是为了防止软件老化而提出的一种主动性、预防性的软件容错策略.把定时Rejuvenation策略引入到执行时间有限的单任务程序中,分析了存在老化时,软件Rejuvenation对程序总执行时间所起的影响.仿真结果表明,定时的Rejuvenation策略能有效地避免软件老化带来的突发性失效,从而减少程序的执行时间.

软件Rejuvenation周期的优化

软件Rejuvenation技术通过定期对软件系统执行Rejuvenation操作来有效改善软件系统性能,执行Rejuvenation操作的周期直接关系到软件系统性能的改善.目前,主要通过单个指标的优化对软件Rejuvenation周期进行研究,这并不能整体提高性能.鉴于此,提出了综合考虑整体性能指标的方法来优化软件Rejuvenation周期,将所关心的指标综合考虑,在适当降低某个指标性能的同时有效提高其他指标性能,起到整体提高软件系统性能的目的.以综合考虑系统可用性、系统停机时间以及系统损失3个指标为例进行探讨,并寻找到了2个可以用之对软件Rejuvenation周期进行优化的目标函数.实验结果表明,通过该目标函数得到的周期可以很好地改善软件系统的整体性能.

The Purification and Rejuvenation and Breeding Technology of Rice Variety Yunjing 29 with Fragrant and Soft Rice

Yunjing 29 bred by Institute of Food Crops of Yunnan Academy of Agricultural Sciences is a new conventional japonica rice variety with fragrant and sof rice, it was examined and approved by Yunnan Provincial Variety Examination and Approval Committee in March of 2011. The variety is early-maturing, high-yield and good-quality, and has resistances to lodging, blast and bacterial leaf blight, its rice is sweet soft, goluptious and lucidus as well as not coarse when it is cold, so it is a good commodity. To further promote the popularization and cultivation of the variety, maintain its characteristics of high quality and high yield, and prevent commingling and degeneration, the purification and rejuvenation as well as breeding technology of high-quality seeds were proposed after continuous exploration and study.

基于检查点和Rejuvenation的软件运行模型分析

近来的研究表明 ,长时间运行的通信软件往往存在老化现象。为防止软件老化引起的突发性系统停机 ,提高系统的可靠性 ,人们提出了一种预防性的软件容错策略 ,称为rejuvenation。由于它的过程复杂 ,总的停机成本仍然是可观的。检查点是一种轻量级的软件容错策略 ,其成本远小于rejuvenation的成本。该文通过合理结合rejuvenation和检查点技术 ,实现了降低总的系统停机成本的目的。文中给出了系统的Petri网模型 ,并结合实例进行了分析。

Rejuvenation of Fossil Sutures and Related Mesozoic Intracontinental Orogenies in South China

The Huanan (South China) subcontinent was created by amalgamation of the Yangtze, Xianggan, Cathaysia and Zhemin microcontinents by the Guangxi orogeny in the Early Palaeozoic. The closure of the Tethyan Ocean and subsequent collision event outside the amalgamated continent reactivated fossil sutures and resulted in intracontinental (ensialic) orogenies in the Mesozoic. Based on evidence from deformation, molasse and granitoids, the Sichuan-Guizhou-Hunan—southern Hubei and Hunan-Jiangxi-Fujian Yanshanian fold-thrust systems and the Lower Yangtze-northwestern Fujian Indosinian fold-thrust system are thought to be intracontinental orogens. Their main features are as follows: intracontinental orogenies occurred areally, thrusting propagated towards the interior of the continental, they extend parallelly to the strikes of the fossil sutures, and the details of the temporal-spatial evolution of the orogens depend on subduction-collision events.

Compound Rejuvenation of Polymer Modified Asphalt Binder

The objective of this research was to show a way to conduct rejuvenation of aged polymer modified asphalt binder（PMB） successfully.To fully evaluate and understand the rejuvenation of aged PMB,the Penetration grade tests including penetration,soften point,ductility and elastic recovery and SuperpaveTM PG grade tests including DSR,BBR and DDT were conducted.The rejuvenation effect of aged PMB by utilizing a fluid recycling agent in common use for binder rejuvenation was evaluated.And then the compound rejuvenation effect of aged PMB by utilizing the recycling agent with a new modifying additive for binder modification was evaluated.The experimental results indicated that the recycling agent in common use currently does not apply to polymer modified asphalt binder rejuvenation.But the recycling agent together with the modifying additive can restore the characteristics of aged polymer modified binder very well.Therefore,compound rejuvenation of polymer modified asphalt binder is recommended.

Structural evolution in deformation-induced rejuvenation in metallic glasses: A cavity perspective

Classical molecular dynamics simulations have been performed to investigate the structural evolution in deformationinduced rejuvenation in Cu_(80)Zr_(20) metallic glass. Metallic glasses obtained by different cooling rates can be rejuvenated into the glassy state with almost the same potential energy by compressive deformation. The aging effect in different metallic glasses in cooling process can be completely erased by the deformation-induced rejuvenation. The evolution of cavities has been analyzed to understand the structural evolution in rejuvenation. It is found that as metallic glasses are rejuvenated by mechanical deformation, a lot of cavities are created. The lower the potential energy is, the more the cavities are created. The cavities are mainly created in the regions without cavities or with small cavities populated, indicating that the irreversible rearrangements induced by deformation are accompanied by the creation of cavity. This finding elucidates the underlying structural basis for rejuvenation and aging in metallic glasses from the cavity perspective.

Preventing aging with stem cell rejuvenation:Feasible or infeasible?

Characterized by dysfunction of tissues, organs, organ systems and the whole organism, aging results fromthe reduced function of effective stem cell populations. Recent advances in aging research have demonstrated that old tissue stem cells can be rejuvenated for the purpose of maintaining the old-organ function by youthful re-calibration of the environment where stem cells reside. Biochemical cues regulating tissue stem cell function include molecular signaling pathways that interact between stem cells themselves and their niches. Historically, plasma fractions have been shown to contain factors capable of controlling age phenotypes; subsequently, signaling pathways involved in the aging process have been identified. Consequently, modulation of signaling pathways such as Notch/Delta, Wnt, transforming growth factor-β, JAK/STAT, mammalian target of rapamycin and p38 mitogen-activated protein kinase has demonstrated potential to rejuvenate stem cell function leading to organismic rejuvenation. Several synthetic agents and natural sources, such as phytochemicals and flavonoids, have been proposed to rejuvenate old stem cells by targeting these pathways. However, several concerns still remain to achieve effective organismic rejuvenation in clinical settings, such as possible carcinogenic actions; thus, further research is still required.

Tendon stem/progenitor cell ageing: Modulation and rejuvenation

Tendon ageing is a complicated process caused by multifaceted pathways and ageing plays a critical role in the occurrence and severity of tendon injury.The role of tendon stem/progenitor cells(TSPCs)in tendon maintenance and regeneration has received increasing attention in recent years.The decreased capacity of TSPCs in seniors contributes to impaired tendon functions and raises questions as to what extent these cells either affect,or cause ageing,and whether these age-related cellular alterations are caused by intrinsic factors or the cellular environment.In this review,recent discoveries concerning the biological characteristics of TSPCs and age-related changes in TSPCs,including the effects of cellular epigenetic alterations and the mechanisms involved in the ageing process,are analyzed.During the ageing process,TSPCs ageing might occur as a natural part of the tendon ageing,but could also result from decreased levels of growth factor,hormone deficits and changes in other related factors.Here,we discuss methods that might induce the rejuvenation of TSPC functions that are impaired during ageing,including moderate exercise,cell extracellular matrix condition,growth factors and hormones;these methods aim to rejuvenate the features of youthfulness with the ultimate goal of improving human health during ageing.

Application Server Aging Model and Multi-Level Rejuvenation Strategy Using Semi-Markov Process

Aiming at the characteristic of the dependency between the application components and the application server platform, a rejuvenation strategy with two different levels of rejuvenation granularities is put forward in this paper including the application component reiuvenation and the application server system rejuvenation. The availability and maintenance cost functions are obtained by means of establishing the application server aging model and the boundary condition of the optimal rejuvenation time is analyzed. Theory analysis indicates that the two-level rejuvenation strategy is superior to the traditional single level one. Finally, evaluation experiments are carried out and numerical result shows that compared with the traditional rejuvenation policy, the rejuvenation strategy proposed in this paper can further increase availability of the application server and reduce maintenance cost.

Fractional Ablative Radio-Frequency Resurfacing in Asian and Caucasian Skin: A Novel Method for Deep Radiofrequency Fractional Skin Rejuvenation

This paper reports the clinical experience of a multi-center, multiple physician trial with a novel fractional radiofrequency ablative skin resurfacing and rejuvenation device (Fractora, Invasix, Israel) deployed on both Caucasian skin types I - III and Asian skin type IV. Histological study demonstrated deep ablation and collagen restructuring in the papillary and reticular dermis. The Fractora device combines the more “cone shaped” ablation seen with CO2 and Erbium lasers with a deep non-ablative heating pattern, seen with other bipolar RF fractional needle resurfacing devices. Ablation, coagulation zones and healing dynamics are analyzed for different energy settings. Two different treatment protocols are suggested: one for light skin and then one for darker skin with a higher risk of post-inflammatory hypperpigmentation. Treatment results show improvement in skin texture, pores, wrinkles and skin dyschromia.

The Effect of Extract of Oak Gall for Vaginal Tightening and Rejuvenation in Women with Vaginal Relaxation

Background and Objectives: The Quercus (oak) fruit and especially the internal layer of the fruit (jaft) is rich in tannin (polyphenolic compound) and due to its astringent effects on the smooth muscles, we decided to evaluate this effect of extract of Oak Gall for vaginal tightening and rejuvenation in women with vaginal relaxation. Methods: This double blind clinical trial was performed on women with vaginal relaxation at Asali Hospital in Khorramabad from May to October 2012 and was candidate for colporrhaphy due to different types of pelvic organ prolapse. Sampling method was done on nonprobability basis according to the number of patients referred to clinic. Using blocked random allocation method, the participants were divided into 4 groups of 30 patients (three intervention group and one control group) who were age-matched. Then, an extract of Oak Gall in specified concentrations (10 - 15 and 20 grams in 1.5%, 2% and 2.5% jells) were administered to each intervention group. Finally, the obtained data were analyzed with Chi-square, Pearson, Fisher’s exact test and ANOVA using SPSS software version 19. Results: A total of 120 patients entered into the study with mean age of 36 ± 5.4 years. The evaluation of mean scores showed that sense of sexual satisfaction and orgasm, sense of vaginal tightness during intercourse, sense of vaginal lubrication and loss of vaginal dryness was significantly improved in patients in intervention group. Also other parameters including urinary incontinence, sense of vaginal mass exit, lack of orgasm, sense of air outflow during intercourse, sense of calmness after sex and loss of libido were significantly reduced in the group of patients who received jell 2.5% compared to other groups (P Conclusion: Our findings show that using topical jell of Oak Gall not only is associated with contracting effects, but also improves vaginal lubrication and reduces its dryness. Regarding the lack of previous studies in this field and considering some limitations of this study, we suggest that Oak Gall to be used for management of women with vaginal relaxation.

Peak Power Effect on Skin Rejuvenation Using IPL: Lumecca IPL Evaluation

A high peak power IPL system (Lumecca) was tested to determine the correlation between a high peak power and the successful treatment of pigmented and vascular lesions. Short pulse duration in the millisecond range and high peak power of 3.3 kW/cm2 enabled selective and effective destruction, not only of pigment, but also of vessels in a comparable manner to a pulsed dye laser. Only one treatment session at a low fluence (8 - 16 J/cm2) was sufficient to achieve the desired results.

Epigenetic interventions for brain rejuvenation：anchoring age-related transposons

Highlights：1.Iron and homocysteine accumulation in aging neurons alter genomic methylation.2.The altered methylome reactivates neuronal cell cycle,enabling transposable element mobilization.3.mi R29/p53 axis restores age-related methylation shifts,reactivating neuronal plasticity.4.Augmentation of mi R-29/p53 axis may preempt neurodegenerative disorders.