A new model is presented for hyperglycemia in animals complicated by hyperlipidemia (HD) to study the action of pueraria flavonoids (PF). Kunming mice were treated with alloxan (150 mg/kg) intraperitoneally and fed...A new model is presented for hyperglycemia in animals complicated by hyperlipidemia (HD) to study the action of pueraria flavonoids (PF). Kunming mice were treated with alloxan (150 mg/kg) intraperitoneally and fed a high fat diet. Glucose (Glu), total cholesterol (TC) and triglycerides (TG) in the plasma were measured at week 0, 1, 2, 4, and 8. PF (600, 400, 200 mg/kg) was given to the mice for 14 days beginning from week 1. The effects of PF on the lipase activity and the ability to produce cholesterol and glycogen in the cultured hepatic cells were also tested in vitro. In week 1, 2, 4, and 8 following the final alloxan injection, Glu increased by 763%, 504%, 526%, and 641%;TC increased by 269%, 409%, 449%, and 428%; and TG increased by 415%, 485%, 461%, and 418%. These increases in the model were significant compared with those in normal Kunming mice. PF acted effectively as hypoglycemic and hypolipidemic agents on the mice. The results suggest that the model may be suitable for studies of drugs on diabetes complicated by hyperlipidemia and that PF may be a useful drug for patients with diabetes complicated by hyperlipidemia.展开更多
The kinetics of leaching flavonoids from Pueraria Lobata with ethanol was investigated. The effects of leaching temperature, mechanical agitation rate,concentration of ethanol and feed particle size on leaching kineti...The kinetics of leaching flavonoids from Pueraria Lobata with ethanol was investigated. The effects of leaching temperature, mechanical agitation rate,concentration of ethanol and feed particle size on leaching kinetics were examined.It is found that the smaller the feed particle size or the higher the leaching temperature,the higher the leaching rate. The leaching process can be described by the shrinking-core model. The apparent activation en- ergy is 10.8kJ·mol-1, suggesting that the leaching process is controlled by the inner diffusion. An empirical equa- tion relating the flavonoids leaching rate constant to the feed particle size and leaching temperature was expected.展开更多
Objective To study the optimum extraction and separation process of total flavonoids in the flowers of Flos Puerariae and its antioxidative activity.Methods The total flavonoids were extracted with assistance of ultra...Objective To study the optimum extraction and separation process of total flavonoids in the flowers of Flos Puerariae and its antioxidative activity.Methods The total flavonoids were extracted with assistance of ultrasonic wave and the content was determined at 265 nm wavelength by Spectrophotometric method.Orthogonal experiment L9(34)was carried out to investigate the effects of concentration of solvent,the ratio of material to liquid,time length of extraction,and frequency of extraction on extraction results of total flavonoids from Flos Puerariae.The extracted solution was purified by petroleum ether and ethanol sequently.Under these conditions,the total flavonoids was eluted gradiently with mixed mobile phase of methanol-chloroform solution in the silica gel column system,and then determined by UV scanning and HPLC.Fenton reaction was used to produce and detect hydroxyl radicals(·OH),and pyrogallol system was used to produce and detect the superoxide radical anion(O2-·).Results The optimum conditions were as follows;using 40%(V/V)methanol as extractor with the ratio of material to liquid at 1∶30,and extracting for 2.5 h a time for 3 times.The extraction yield of total flavonoids was 13.6%.Six isoflavone compounds were isolated from the flowers of Flos Puerariae by the method of silica gel column chromatography.Antioxidative test results showed good performance of flavonoid scavenging capacity in both hydroxyl radical system and superoxide radical system and its IC50 was 7.65 μg·mL-1 and 0.18 mg·mL-1,respectively.Conclusions This study provided scientific basis for further development and utilization of Flos Puerariae and make preparation for later pharmacological research.展开更多
[Objectives]To optimize the extraction technology of total flavonoids from Pueraria lobata( Willd.) Ohwi. [Methods]The ultraviolet-visible spectrophotometry was used to determine the content of total flavonoids in Pue...[Objectives]To optimize the extraction technology of total flavonoids from Pueraria lobata( Willd.) Ohwi. [Methods]The ultraviolet-visible spectrophotometry was used to determine the content of total flavonoids in Pueraria lobata( Willd.) Ohwi. With the puerarin as index,the reflux extraction and single factor test were employed to investigate the effects of temperature,time,ethanol concentration and solid-liquid ratio on the content of total flavonoids in Pueraria lobata( Willd.) Ohwi,respectively. Under the optimal extraction technology,the content of total flavonoids in Pueraria lobata( Willd.) Ohwi at different altitudes was determined.[Results] The optimum extraction process was as follows: 70%ethanol; solid-liquid ratio of 1∶ 30; 1 h reflux extraction. Under these conditions,the extraction rate of flavonoids in Pueraria lobata( Willd.) Ohwi was 11. 48%,the total flavonoids content of different kudzu parts was in the order of roots > stems > leaves,and the total flavonoids content of the sample at about an altitude of 1000 m was significantly higher than at the altitudes of 1400 m and 1700 m.[Conclusions]It was suggested that the Pueraria lobata( Willd.) Ohwi should not be cultivated as medicinal plant in too high mountains,and the stems and leaves of Pueraria lobata( Willd.) Ohwi could be used as raw materials for extracting total flavonoids.展开更多
基金Supported by"985"Foundation of Tsinghua University(No.Z0 11)
文摘A new model is presented for hyperglycemia in animals complicated by hyperlipidemia (HD) to study the action of pueraria flavonoids (PF). Kunming mice were treated with alloxan (150 mg/kg) intraperitoneally and fed a high fat diet. Glucose (Glu), total cholesterol (TC) and triglycerides (TG) in the plasma were measured at week 0, 1, 2, 4, and 8. PF (600, 400, 200 mg/kg) was given to the mice for 14 days beginning from week 1. The effects of PF on the lipase activity and the ability to produce cholesterol and glycogen in the cultured hepatic cells were also tested in vitro. In week 1, 2, 4, and 8 following the final alloxan injection, Glu increased by 763%, 504%, 526%, and 641%;TC increased by 269%, 409%, 449%, and 428%; and TG increased by 415%, 485%, 461%, and 418%. These increases in the model were significant compared with those in normal Kunming mice. PF acted effectively as hypoglycemic and hypolipidemic agents on the mice. The results suggest that the model may be suitable for studies of drugs on diabetes complicated by hyperlipidemia and that PF may be a useful drug for patients with diabetes complicated by hyperlipidemia.
基金Hubei Key Lab of Novel Reactor & Green Chemical Technology Open Foundation (No.2004-3) and the In-novative Science Foundation of Hubei Province, China (No.2006ABC014).
文摘The kinetics of leaching flavonoids from Pueraria Lobata with ethanol was investigated. The effects of leaching temperature, mechanical agitation rate,concentration of ethanol and feed particle size on leaching kinetics were examined.It is found that the smaller the feed particle size or the higher the leaching temperature,the higher the leaching rate. The leaching process can be described by the shrinking-core model. The apparent activation en- ergy is 10.8kJ·mol-1, suggesting that the leaching process is controlled by the inner diffusion. An empirical equa- tion relating the flavonoids leaching rate constant to the feed particle size and leaching temperature was expected.
文摘Objective To study the optimum extraction and separation process of total flavonoids in the flowers of Flos Puerariae and its antioxidative activity.Methods The total flavonoids were extracted with assistance of ultrasonic wave and the content was determined at 265 nm wavelength by Spectrophotometric method.Orthogonal experiment L9(34)was carried out to investigate the effects of concentration of solvent,the ratio of material to liquid,time length of extraction,and frequency of extraction on extraction results of total flavonoids from Flos Puerariae.The extracted solution was purified by petroleum ether and ethanol sequently.Under these conditions,the total flavonoids was eluted gradiently with mixed mobile phase of methanol-chloroform solution in the silica gel column system,and then determined by UV scanning and HPLC.Fenton reaction was used to produce and detect hydroxyl radicals(·OH),and pyrogallol system was used to produce and detect the superoxide radical anion(O2-·).Results The optimum conditions were as follows;using 40%(V/V)methanol as extractor with the ratio of material to liquid at 1∶30,and extracting for 2.5 h a time for 3 times.The extraction yield of total flavonoids was 13.6%.Six isoflavone compounds were isolated from the flowers of Flos Puerariae by the method of silica gel column chromatography.Antioxidative test results showed good performance of flavonoid scavenging capacity in both hydroxyl radical system and superoxide radical system and its IC50 was 7.65 μg·mL-1 and 0.18 mg·mL-1,respectively.Conclusions This study provided scientific basis for further development and utilization of Flos Puerariae and make preparation for later pharmacological research.
基金Supported by Sichuan Science and Technology Support Program(2014SZ-0131)
文摘[Objectives]To optimize the extraction technology of total flavonoids from Pueraria lobata( Willd.) Ohwi. [Methods]The ultraviolet-visible spectrophotometry was used to determine the content of total flavonoids in Pueraria lobata( Willd.) Ohwi. With the puerarin as index,the reflux extraction and single factor test were employed to investigate the effects of temperature,time,ethanol concentration and solid-liquid ratio on the content of total flavonoids in Pueraria lobata( Willd.) Ohwi,respectively. Under the optimal extraction technology,the content of total flavonoids in Pueraria lobata( Willd.) Ohwi at different altitudes was determined.[Results] The optimum extraction process was as follows: 70%ethanol; solid-liquid ratio of 1∶ 30; 1 h reflux extraction. Under these conditions,the extraction rate of flavonoids in Pueraria lobata( Willd.) Ohwi was 11. 48%,the total flavonoids content of different kudzu parts was in the order of roots > stems > leaves,and the total flavonoids content of the sample at about an altitude of 1000 m was significantly higher than at the altitudes of 1400 m and 1700 m.[Conclusions]It was suggested that the Pueraria lobata( Willd.) Ohwi should not be cultivated as medicinal plant in too high mountains,and the stems and leaves of Pueraria lobata( Willd.) Ohwi could be used as raw materials for extracting total flavonoids.
