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Puerarin modulation of CENPA affects downstream PLK1 and CCNB1 expression to inhibit bladder cancer cell proliferation
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作者 Hao Xu Wen Gao +7 位作者 Deng Pan Yu-Yang Ma Ruo-Ran Zhang Yong-Li Cao Yu-Chuan Zhou Ming-Yu Xu Pei-Yong Zhang Kun Pang 《Traditional Medicine Research》 2024年第9期32-41,共10页
Background:The treatment alternatives for bladder cancer(BLCA),the 10th most prevalent cancer in the world,need to be further investigated,and many active substances like Puerarin in herbal medicine were found to be e... Background:The treatment alternatives for bladder cancer(BLCA),the 10th most prevalent cancer in the world,need to be further investigated,and many active substances like Puerarin in herbal medicine were found to be effective in treating BLCA.The purpose of this study was to investigate the potential treating mechanisms of Puerarin on BLCA.Methods:The cell counting kit 8 assay and flow cytometry were performed to confirm Puerarin’s ability to suppress BLCA.The differentially expressed proteins(DEPs)were obtained by Tandem Mass Tags technology and functional enrichment analysis performed by R studio.The most enriched pathways were selected for study and the DEPs were screened out.Protein-protein interaction network maps were created using String and Cytoscape and key proteins,which will be analyzed for survival,expression,and upstream transcription factor prediction,were screened out using the cytoHubba plugin.CHEA3 was used to obtain upstream transcription factor validated by molecular docking and western blotting experiments.Results:Cell counting kit 8 showed that Puerarin inhibited BLCA cells,with 50%inhibitory concentration of 218μmol/L in T24 and 198μmol/L in 5637.Flow cytometry reveals that Puerarin blocks T24 and 5637 cells in G1 phase.1,385 DEPs were obtained and the enrichment analysis revealed that cell cycle and DNA replication were the two main areas in which DEPs were enriched.Cyclin-B-cyclin dependent kinase 1(CDK1),cyclin B1(CCNB1),and polo-like kinase 1(PLK1)were identified as key proteins,and their upstream transcription factor was predicted to be centromere protein A(CENPA).Puerarin’s binding energy to CENPA was determined by molecular docking to be−6.3 kcal/mol,indicating a strong binding interaction.Western blot showed that Puerarin significantly reduced the expression of CENPA.Conclusion:We hypothesize that Puerarin may inhibit the proliferation of bladder cancer cells by inhibiting CENPA expression to regulate PLK1 and CCNB1 expression,thereby affecting cell cycle. 展开更多
关键词 puerarin BLCA Tandem Mass Tags cell cycle
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Puerarin mediated miR-30b-5p targeting fibroblast activation protein against oral submucous fibrosis
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作者 SAIFEI XIE HUI XIE +4 位作者 JINCAI GUO JIN TAN YULIN YU MINYI ZHANG SHANG WEN 《BIOCELL》 SCIE 2024年第4期591-599,共9页
Background:Puerarin(Pue)has been reported to be a natural active ingredient with multiple antifibrotic properties.This work aimed at exploring the function of Pue in oral submucousfibrosis(OSF)treatment.Methods:Human or... Background:Puerarin(Pue)has been reported to be a natural active ingredient with multiple antifibrotic properties.This work aimed at exploring the function of Pue in oral submucousfibrosis(OSF)treatment.Methods:Human oral mucosafibroblasts(hOMF)were induced with transforming growth factor beta1(TGF-β1)and intervened with Pue.Expressions offibrosis-related markers were analyzed by Western blot and IF staining.Cell viability was characterized by the CCK-8 assay.Expressions of miR-30 family members were quantified by qRT-PCR.The correlation betweenfibroblast activation protein(FAP)and miR-30 family expression was evaluated by the Pearson correlation coefficient.Bioinformatics prediction and dual-luciferase reporter assay were employed to verify the regulation between FAP and miR-30b-5p.The specific mechanism of Pue on OSF was explored through the promotion or inhibition of miR-30b-5p.Results:After induction by TGF-β1,hOMF showed upregulated Collagen I,Collagen III,and FAP expressions,while miR-30 family expression was downregulated with miR-30b-5p being the most significant.Pue intervention inhibited the excessive proliferation of TGF-β1-induced hOMF,downregulated FAP,collagen type 3(COL3A1),collagen type 1(COL1A1),matrix metalloproteinase 1(MMP1),and matrix metalloproteinase 3(MMP3)expressions,and restored miR-30 family expression.Bioinformatics prediction and dual-luciferase reporter assay revealed that miR-30b-5p selectively inhibited FAP expression.Mechanistically,miR-30b-5p mimic suppressed the excessive proliferation of TGF-β1-induced hOMF and declinedfibrosis levels.Pue intervention significantly reversed the promotion of TGF-β1-induced OSF by miR-30b-5p inhibition.Conclusion:Pue mediated miR-30b-5p targeting FAP against OSF,which provided a theoretical basis for the pathogenesis research and Pue application in OSF. 展开更多
关键词 puerarin miR-30b-5p FAP Oral submucous fibrosis
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Puerarin逆转K562/AO2耐药的分子机制 被引量:1
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作者 陈进伟 陶师 +2 位作者 骆蓉 张广森 徐运孝 《中南大学学报(医学版)》 CAS CSCD 北大核心 2008年第3期216-221,共6页
目的:研究黄酮类化合物puerarin对K562/AO2(人红白血病多药耐药细胞系)细胞耐药逆转作用的分子机制。方法:免疫荧光染色方法检测阿霉素(ADR)和puerarin对K562(人红白血病细胞系)和K562/AO2两种细胞NF-κB活性的影响;免疫细胞化学染色方... 目的:研究黄酮类化合物puerarin对K562/AO2(人红白血病多药耐药细胞系)细胞耐药逆转作用的分子机制。方法:免疫荧光染色方法检测阿霉素(ADR)和puerarin对K562(人红白血病细胞系)和K562/AO2两种细胞NF-κB活性的影响;免疫细胞化学染色方法检测ADR和puerarin对K562和K562/AO2的survivin表达的影响;流式细胞仪检测ADR和puerarin对K562和K562/AO2的p-gp表达的影响。结果:经ADR处理后的K562细胞和K562/AO2细胞NF-κB的活性明显高于K562细胞空白对照组;经puerarin预处理后再加ADR处理的K562细胞中的NF-κB的活性明显低于只用ADR处理的K562细胞。经puerarin干预后的K562/AO2细胞的NF-κB的活性明显低于未经puerarin干预的K562/AO2细胞;经ADR处理后的K562细胞和K562/AO2细胞的p-gp,survivin表达明显高于K562细胞空白对照组;经puerarin预处理后再加ADR处理的K562细胞中的p-gp,survivin表达明显低于未经pu-erarin预处理的K562细胞;经puerarin干预后的K562/AO2细胞的p-gp,survivin表达明显低于未经puer-arin干预的K562/AO2细胞。p-gp和survivin表达呈正相关。结论:NF-κB的活化使p-gp和survivin表达增多可能是K562细胞多药耐药形成的机制之一。Puerarin能预防和阻止K562细胞耐药形成,并能逆转K562/AO2对ADR的耐药,其机制与抑制NF-κB活性及survivin和p-gp的表达有关。 展开更多
关键词 puerarin K562/A02 药耐药 逆转 分子机制
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葛根素治疗糖尿病文献计量学分析
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作者 晏芙蓉 门花娟 +6 位作者 余丽 王怡帆 闫浩 李小蓉 史永恒 左振宇 刘靖丽 《现代中医药》 2025年第1期12-22,共11页
目的梳理葛根素治疗糖尿病领域研究热点及发展趋势,为后续研究提供参考。方法以中国知网(CNKI)和Web of Science Core Collection(WOSCC)为数据库,检索2003—2023年相关文献,采用CiteSpace对样本文献的发文量、作者、国家/地区、研究机... 目的梳理葛根素治疗糖尿病领域研究热点及发展趋势,为后续研究提供参考。方法以中国知网(CNKI)和Web of Science Core Collection(WOSCC)为数据库,检索2003—2023年相关文献,采用CiteSpace对样本文献的发文量、作者、国家/地区、研究机构、关键词等进行可视化分析。结果研究共纳入中文文献755篇,英文文献199篇,中文呈下降趋势,英文呈上升趋势。中、英文发文最多的作者分别是李强翔、Kim Jin Sook和Du Guanhua。中国是发文最多的国家。中南大学湘雅医院内分泌科和北京中医药大学是中文发文量最多的机构,上海中医药大学是英文发文量最多的机构。中文文献的关键词、聚类、突现词图谱显示葛根素制剂、糖尿病及糖尿病并发症、药理作用、临床应用是主要研究方向。英文文献的关键词、聚类、突现词图谱显示其主要围绕葛根素制剂治疗糖尿病及其并发症的药理作用和作用机制研究。结论葛根素治疗糖尿病研究总发文量呈稳定趋势。核心作者、国家/地区、研究机构以中国学者和研究机构为主,交流合作有待加强。中英文研究方向各有侧重,从分子水平深入研究葛根素治疗糖尿病及其并发症的生物学作用机制是共同的研究热点和趋势。 展开更多
关键词 葛根素 糖尿病 文献计量分析 知识图谱 可视化分析
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高效液相色谱法同时测定骨痹灵片中5种指标成分
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作者 丁爱华 朱干红 +4 位作者 曹桂萍 陈玲玲 滕习羽 支荣荣 周会芹 《化学分析计量》 2025年第1期100-105,共6页
建立高效液相色谱法同时测定骨痹灵片中葛根素、阿魏酸、橙皮苷、蛇床子素和异欧前胡素。采用CAPCELL PAK C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,流量为1.0 mL/min,柱温为30℃,检测波长为250... 建立高效液相色谱法同时测定骨痹灵片中葛根素、阿魏酸、橙皮苷、蛇床子素和异欧前胡素。采用CAPCELL PAK C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱,流量为1.0 mL/min,柱温为30℃,检测波长为250 nm(葛根素)、284 nm(橙皮苷)和322 nm(阿魏酸、蛇床子素、异欧前胡素),进样体积为10μL。5种成分在各自质量浓度范围内与对应的色谱峰面积线性关系良好,相关系数均为0.9999,方法检出限为0.04~0.14μg/mL,定量限为0.13~0.46μg/mL。样品加标平均回收率为96.0%~103.1%,测定结果的相对标准偏差为0.8~1.7%(n=6)。该方法简便可靠,重复性好,可用于骨痹灵片的质量控制。 展开更多
关键词 骨痹灵片 高效液相色谱法 葛根素 阿魏酸 橙皮苷 蛇床子素 异欧前胡素
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葛根素对急慢性肝损伤的保护作用及机制研究进展
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作者 高靖雯 秦燕 《医学综述》 CAS 2025年第1期18-22,共5页
肝损伤是一种常见的临床疾病,可分为急性肝损伤和慢性肝损伤,发病率和死亡率均较高,是我国乃至世界范围内的重大健康问题。葛根是一种药食同源植物,其重要成分为葛根素,具有多种生物学作用,如抗氧化、抗炎、抑制细胞凋亡。近年来关于葛... 肝损伤是一种常见的临床疾病,可分为急性肝损伤和慢性肝损伤,发病率和死亡率均较高,是我国乃至世界范围内的重大健康问题。葛根是一种药食同源植物,其重要成分为葛根素,具有多种生物学作用,如抗氧化、抗炎、抑制细胞凋亡。近年来关于葛根素在肝脏疾病方面的研究不断增多,其可以通过改善炎症反应、降低氧化应激、减少细胞凋亡等发挥肝脏保护作用。因此,深入了解葛根素在急慢性肝损伤中的保护作用及分子机制有助于探索新的治疗策略。 展开更多
关键词 急性肝损伤 慢性肝损伤 葛根素 治疗作用
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高冠平胶囊的质量标准提升研究
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作者 赵利娟 李夕 +6 位作者 李晔 任建平 李晓杰 程绪钊 刘小卫 王蓓 张俏 《西北药学杂志》 2025年第1期83-89,共7页
目的 提升高冠平胶囊的质量标准,确保临床应用的安全性和有效性。方法 采用薄层色谱法(thin layer chromatography,TLC)鉴别高冠平胶囊中的葛根素和丹参酮ⅡA;采用高效液相色谱法(high performance liquid chromatography,HPLC)测定羟... 目的 提升高冠平胶囊的质量标准,确保临床应用的安全性和有效性。方法 采用薄层色谱法(thin layer chromatography,TLC)鉴别高冠平胶囊中的葛根素和丹参酮ⅡA;采用高效液相色谱法(high performance liquid chromatography,HPLC)测定羟基红花黄色素A、葛根素和3’-甲氧基葛根素的含量。结果 高冠平胶囊中的葛根素和丹参酮ⅡA的TLC特征斑点清晰可见,分离效果好,且阴性样品无干扰。HPLC测定结果显示,羟基红花黄色素A、葛根素、3’-甲氧基葛根素分离度良好,质量浓度分别在相应范围内(1.63~32.61、5.94~118.79、4.90~97.90μg·mL^(-1))均与其峰面积线性关系良好(r≥0.999 8);平均加样回收率为98.85%~100.38%,相对标准偏差(relative standard deviation,RSD)均小于1.5%。结论 建立的高冠平胶囊TLC定性鉴别和HPLC定量测定方法简便、准确,专属性和重复性好,可用于提升高冠平胶囊的质量标准,加强其质量控制。 展开更多
关键词 高冠平胶囊 薄层色谱法 高效液相色谱法 葛根素 丹参酮ⅡA 羟基红花黄色素A 3’-甲氧基葛根素
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葛根素调控NLRP3/Caspase-1/GSDMD通路改善酒精性肝病的机制及其对细胞焦亡的影响
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作者 高靖雯 刘乐 +1 位作者 张艺馨 秦燕 《中医药信息》 2025年第1期19-24,共6页
目的:观察葛根素对酒精性肝病小鼠细胞焦亡的影响,同时基于NLRP3/Caspase-1/GSDMD信号通路初步探讨其作用机制。方法:60只雄性C57BL/6J小鼠,随机分为正常对照组、酒精性肝病模型组和葛根素低、中、高剂量组(100、200、400 mg/kg),除正... 目的:观察葛根素对酒精性肝病小鼠细胞焦亡的影响,同时基于NLRP3/Caspase-1/GSDMD信号通路初步探讨其作用机制。方法:60只雄性C57BL/6J小鼠,随机分为正常对照组、酒精性肝病模型组和葛根素低、中、高剂量组(100、200、400 mg/kg),除正常对照组外,其余各组采用酒精灌胃法构建小鼠酒精性肝病模型,1 h后正常对照组、酒精性肝病模型组灌胃给予生理盐水,葛根素低、中、高剂量组灌胃给予相应浓度的葛根素,持续干预8周。收集小鼠血清和肝组织,检测血清中ALT及AST水平,采用qRT-PCR和Western blot检测肝组织炎症因子、焦亡通路相关因子的mRNA和蛋白表达水平,HE染色观察小鼠肝组织病理变化。结果:与正常对照组比较,酒精性肝病模型组ALT、AST水平显著升高(P<0.01,P<0.001),TNF-α、NF-κB、IL-18、IL-1βmRNA表达水平均明显升高(P<0.01,P<0.001),NLRP3、Caspase-1、GSDMD mRNA和蛋白表达水平都明显升高(P<0.01,P<0.001);与酒精性肝病模型组比较,葛根素中、高剂量组小鼠血清ALT、AST水平,TNF-α、NF-κB、IL-18、IL-1βmRNA表达水平,及细胞焦亡相关因子NLRP3、Caspase-1、GSDMD mRNA和蛋白表达水平都有不同程度下降(P<0.05,P<0.01),HE染色肝组织病理变化与上述结果一致。结论:小鼠酒精性肝病的发生与细胞焦亡有关,而葛根素可以有效抑制小鼠酒精性肝病的细胞焦亡从而减轻肝脏损害,其作用可能是通过NLRP3/Caspase-1/GSDMD通路实现的。 展开更多
关键词 葛根素 酒精性肝病 NLRP3 CASPASE-1 GSDMD
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Inhibition of Excitatory Amino Acid Efflux Contributes to Protective Effects of Puerarin Against Cerebral Ischemia in Rats 被引量:30
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作者 XIAO-HONG XU XIAO-XIANG ZHENG +1 位作者 QIONG ZHOU HUI LI 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2007年第4期336-342,共7页
To investigate whether the protective effects of puerarine (Pur) against cerebral ischemia is associated with depressing the extracellular levels of amino acid transmitters in brain of rats. Methods Male Sprague-Daw... To investigate whether the protective effects of puerarine (Pur) against cerebral ischemia is associated with depressing the extracellular levels of amino acid transmitters in brain of rats. Methods Male Sprague-Dawley rats were subjected to transient middle cerebral artery occlusion (MCAO) for 60 min followed by 24 h reperfusion. Put (50, 100 mg/kg, i.p.) was administered at the onset of MCAO. The infarct rate and edema rate were detected on TTC (2,3,5-triphenyltetrazolium chloride)-stained coronal sections. The extracellular levels of amino acid transmitters were monitored in striatum of rats with ischemic/reperfusion injury using in vivo microdialysis technique. Furthermore, the protective effects of Pur against glutamate-induced neurotoxicity were detected. Glutamate-induced apoptotic and necrotic cells in hippocampus were estimated by flow cytometric analysis of Annexin-V and PI labeling cells. Results Pur (100 mg/kg) significantly decreased infarct size by 31.6% (P〈0.05), reduced edema volume (P〈0.05), and improved neurological functions (P〈0.05) following MCAO. In these rats, the ischemia-induced extracellular levels of aspartate (Asp), glutamate (Glu), γ-aminobutyric acid (GABA), and taurine (Tau) were significantly reduced in striatum of vehicle-treated animals by 54.7%, 56.7%, 75.8%, and 68.1% (P〈0.01 and P〈0.05). Pur reduced the peak values of Glu and Asp more obviously than those of GABA and Tau, and the rate of Glu/GABA during MCAO markedly decreased in Pur-treated MCAO rats, compared with the vehicle-treated MCAO rats. Meanwhile, apoptosis and necrosis induced by Glu in cultured hippocampal neurons were significantly reduced after Pur treatment. Conclusion Acute treatment with Put at the onset of occlusion significantly depresses ischemia-induced efflux of amino acids, especially, excitotoxicity in the striatum, a mechanism underlying the neuroprotective effect on cellular survival. 展开更多
关键词 Cerebral ischemia puerarin MICRODIALYSIS Amino acid transmitter NEUROPROTECTION
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Puerarin enhances superoxide dismutase activity and inhibits RAGE and VEGF expression in retinas of STZ-induced early diabetic rats 被引量:17
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作者 Fang Chen Hong-Quan Zhang +6 位作者 Jun Zhu Kai-Yang Liu Hong Cheng Guo-Li Li Shan Xu Wei-Hong Lv Zheng-Gao Xie 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2012年第11期891-896,共6页
Objective:To investigate the effects of puerarin on the activity of superoxide dismutase(SOD), and expressions of advanced glycation end-product(AGE) receptor(RAGE) and vascular endothelial growth factor(VEGF) in reti... Objective:To investigate the effects of puerarin on the activity of superoxide dismutase(SOD), and expressions of advanced glycation end-product(AGE) receptor(RAGE) and vascular endothelial growth factor(VEGF) in retinas of streptozotocin(STZ)-induced early diabetic rats. Methods:Diabetic rat models were established by inducing diabetes via intra-peritoneal injection of STZ.Rats were randomly divided into normal(control),diabetic(DM),and DM+ puerarin groups.After intra-gastric administration of puerarin(500 mg/kg/day for 4 weeks),levels of SOD and malondialdehyde(MDA) were determined in serum and retina.mRNA and protein expression levels of RAGE and VEGF in retinas were determined by real-lime polymerase chain reaction(RT-PCR)(mRNA) and Western blot analysis(protein levels).Results:There was significantly lower SOD activity and significantly higher MDA in serum and retinas of the DM group compared with the two other groups(P【0.05).After treatment with puerarin,SOD activity increased and MDA content decreased in this group(P【0.05).mRNA and protein expression levels of RACE and VECF in the DM group were significantly higher than those of the other groups (P【0.05),and decreased after puerarin treatment(P【0.05).Conclusions:Puerarin is able to enhance SOD activity,and inhibit RAGE and VEGF expressions in retinas of STZ-induced early diabetic ruts. 展开更多
关键词 DIABETIC RETINOPATHY puerarin Oxidative stress Receptor for advanced glycation end products VASCULAR ENDOTHELIAL growth factor
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Mechanism of combined use of vitamin D and puerarin in anti-hepatic fibrosis by regulating the Wnt/β-catenin signalling pathway 被引量:15
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作者 Gan-Rong Huang Si-Jun Wei +3 位作者 Yan-Qiang Huang Wei Xing Lu-Yao Wang Ling-Ling Liang 《World Journal of Gastroenterology》 SCIE CAS 2018年第36期4178-4185,共8页
AIM To reveal the protective mechanism of the combined use of vitamin D and puerarin in the progression of hepatic fibrosis induced by carbon tetrachloride(CCl4).METHODS Eight-week-old male Wistar rats were randomly d... AIM To reveal the protective mechanism of the combined use of vitamin D and puerarin in the progression of hepatic fibrosis induced by carbon tetrachloride(CCl4).METHODS Eight-week-old male Wistar rats were randomly divided into a normal control group(C group), a CCl4 group(CCl4 group), a vitamin D group(V group), a puerarin group(P group), and a combined group of vitamin D and puerarin(V + P group), each of which contained ten rats. In this way, we built a rat model of CCl4-induced hepatic fibrosis with intervention by vitamin D, puerarin, or a combination of the two. After eight weeks, the mice were sacrificed to collect serum and liver specimens. Blood was collected to detect the hyaluronic acid(HA). We also measured hydroxyproline(Hyp) and prepared paraffin sections of liver. After Sirius red staining, the liver specimens were observed under a microscope. RT-PCR and western blot analysis were adopted to detect the mRNA and the proteinlevels of Collagen I, Collagen III, Wnt1, and β-catenin in the liver tissues, respectively.RESULTS Hepatic fibrosis was observed in the CCl4 group. In comparison, hepatic fibrosis was attenuated in the V, P, and V + P groups: the HA level in blood and the Hyp level in liver were reduced, and the mRNA levels of Collagen I, Collagen III, Wnt, and β-catenin in liver were also decreased, as well as the protein levels of Wnt1 and β-catenin. Among these groups, the V + P group demonstrated the greatest amelioration of hepatic fibrosis.CONCLUSION The combined application of vitamin D and puerarin is capable of alleviating CCl4-induced hepatic fibrosis of rats. As to the mechanism, it is probably because the combined use is able to silence the Wnt1/β-catenin pathway, suppress the activation of hepatic stellate cells, and reduce the secretion of collagen fibers, therefore improving the anti-hepatic fibrosis effect. 展开更多
关键词 Carbon TETRACHLORIDE HEPATIC FIBROSIS VITAMIN D puerarin WNT/Β-CATENIN
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Pharmacokinetic and pharmacodynamic analysis of ferulic acidpuerarin-astragaloside in combination with neuroprotective in cerebral ischemia/reperfusion injury in rats 被引量:5
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作者 Li-Jun Ge Shou-Yan Fan +6 位作者 Jie-Hong Yang Yi Wei Zhen-Hong Zhu Yi-Jia Lou Ying Guo Hai-Tong Wan Yi-Qiang Xie 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2015年第4期299-304,共6页
Objective:To investigate the effects of the active ingredients combined therapy on inflammatory factors interleukin 1 beta(IL-1β)and neuropeptide Y(NPY)based on pharmacodynamics in rats.Methods:The animal model was b... Objective:To investigate the effects of the active ingredients combined therapy on inflammatory factors interleukin 1 beta(IL-1β)and neuropeptide Y(NPY)based on pharmacodynamics in rats.Methods:The animal model was built by transient middle cerebral artery occlusion(MCAO).The method for evaluating the concentrations of the FA-Pr-AI components in rat plasma was established by using HPLC and the expression levels of IL-1βand NPY were determined by ELISA.A new mathematics method of the trend of percentage rate of change(PRC)was used to assess the correlation between pharmacokinetics(PK)and pharmacodynamics(PD).Results:FA-Pr-Al in combination reduced neurological deficits,decreased infarct volume and inhibited the expression levels of IL-1βand NPY(all P<0.05)compared with the model group.FA,Pr and Al all displayed two compartment open models in rats.Clockwise hysteresis loops were obtained by time-concentration-effect curves.IL-1βand NPY level changes in the plasma followed an opposite trend to the plasma concentration tendency after C_(max)was reached.Astragaloside's PRC value was significantly higher than those of FA and puerarin between 120 to 180 min.Conclusions:The pharmacokinetics of FA-PrAl in combination were closely related its pharmacodynamics in treating ischemia/reperfusion injury,and the components of FA-Pr-Al may have a synergistic pharmacological effect.Astragaloside may play a more pronounced role in regulating IL-1βand NPY levels compared with puerarin or FA. 展开更多
关键词 Ferulic acid puerarin ASTRAGALOSIDE INTERLEUKIN-1Β NEUROPEPTIDE Y
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A Novel Conformation Investigation on Newly Synthesized Compound of Diethyl Puerarin-7-yl Phosphate 被引量:6
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作者 袁金伟 陈晓岚 +3 位作者 屈凌波 唐明生 梁瑞玲 赵玉芬 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2006年第1期78-84,共7页
A novel compound, diethyl puerarin-7-yl phosphate, was synthesized through a simplified Atheron-Todd reaction for the first time. The structure of this compound was elucidated by IR, ESI-MS and NMR. Two conformations ... A novel compound, diethyl puerarin-7-yl phosphate, was synthesized through a simplified Atheron-Todd reaction for the first time. The structure of this compound was elucidated by IR, ESI-MS and NMR. Two conformations of the compound were testified by 2D NMR (HSQC and HMBC) and dynamic NMR. Furthermore, we carried out the conformational analysis using chemical calculation by the Gaussian 03. Finally, we obtained two preferred conformations and energy values. 展开更多
关键词 puerarin synthesis chemical calculation conformation group
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Compound of icariin,astragalus,and puerarin mitigates iron overload in the cerebral cortex of Alzheimer's disease mice 被引量:13
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作者 Yu Zhang Wei-Na Kong Xi-Qing Chai 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第4期731-736,共6页
Increasing evidence indicates that disruption of normal iron homeostasis may contribute to pathological development of Alzheimer's disease.Icariin,astragalus,and puerarin have been shown to suppress iron overload in ... Increasing evidence indicates that disruption of normal iron homeostasis may contribute to pathological development of Alzheimer's disease.Icariin,astragalus,and puerarin have been shown to suppress iron overload in the cerebral cortex and improve spatial learning and memory disorders in Alzheimer's disease mice,although the underlying mechanism remains unclear.In the present study,APPswe/PS1ΔE9 transgenic mice were administered icariin,astragalus,and puerarin(120,80,and 80 mg/kg,respectively,once a day,for 3 months).Iron levels were detected by flame atomic absorption spectroscopy.Interleukin-1β,interleukin-6,and tumor necrosis factor-α levels were measured in the cerebral cortex by enzyme linked immunosorbent assay.Glutathione peroxidase and superoxide dismutase activity and malondialdehyde content were determined by colorimetry.Our results demonstrate that after treatment,iron levels and malondialdehyde content are decreased,while glutathione peroxidase and superoxide dismutase activities are increased.Further,interleukin-1β,interleukin-6,and tumor necrosis factor-α levels were reduced.These results confirm that compounds of icariin,astragalus,and puerarin may alleviate iron overload by reducing oxidative stress and the inflammatory response. 展开更多
关键词 nerve regeneration Alzheimer's disease iron overload ICARIIN ASTRAGALUS puerarin oxidative stress inflammatory response neural regeneration
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Restrictive Effect of Puerarin on Myocardial Infarct Area in Dogs and Its Possible Mechanism 被引量:5
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作者 刘启功 陆再英 王琳 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2000年第1期43-45,共3页
Summary: To evaluate the protective effect of puerarin on ischemic myocardium in dogs with acute myocardial infarction (AMI) and to reveal its possible mechanism, 10 dogs were randomly divided into puerarin group (gro... Summary: To evaluate the protective effect of puerarin on ischemic myocardium in dogs with acute myocardial infarction (AMI) and to reveal its possible mechanism, 10 dogs were randomly divided into puerarin group (group G) and control group (group C). AMI model was established in all dogs. Puerarin or saline was administered over a period of 21 days. Coronary angiography was performed before and after ligation of coronary artery. Eight hemorheological parameters were examined before and 22 days after the operation. The infarct area and vessel density of myocardium were assessed. The infarct area in group G was smaller than that in group C. Angiography 2 h and 22 d after ligation of coronary artery revealed significant augmentation of collateral vessels in group G as compared with control group. The platelet aggregation and the blood viscosity were in- creased during AMI when compared with control phase, and the increased indexes during AMI would be inhibited when puerarin were given. Capillaries and distribution vessel density in is- chemic zone on day 22 showed statistically significant augmentation in group G as compared with control group. Puerarin might improve the opening and formation of coronary collateral circula- tion, and might inhibit the increase of platelet aggregation and the blood viscosity during AMI, and thereby improve microcirculation and restrict myocardial infarct area. 展开更多
关键词 puerarin collateral circulation HEMORHEOLOGY myocardial infarction
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Experimental Study on the Protective Effect of Puerarin to Parkinson Disease 被引量:5
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作者 李雪莉 孙圣刚 童萼塘 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2003年第2期148-150,共3页
The protective effect of puerarin on the Parkinson disease (PD) mice with decreased estrogen level was investigated in order to develop a new potential medicine as a substitute for estrogen for preventing and treating... The protective effect of puerarin on the Parkinson disease (PD) mice with decreased estrogen level was investigated in order to develop a new potential medicine as a substitute for estrogen for preventing and treating PD. By using immunohistochemical method of avidinbiotin peroxidase complex (ABC), the distribution of the cells positive for tyrosine hydroxylase (TH) and fibres in the substantia nigra of the mouse were observed. These mice were divided into three groups randomly: group A , normal-female-mouse models; group B containing three subgroups, B1 (normal saline) , B2 (estrogen), B3 (puerarin); group C containing three sub groups, C1 (normal saline), C2 (estrogen), C3 (puerarin). By using TUNEL the numbers of apoptosis cells in every visual field was counted and the difference between the experimental group and control group was compared. The results showed the numbers of the cells positive for TH were more and the numbers of apoptosis cells were less in the normal-female-mouse models group than in the group of model made after ovar-iosteresis and the group of model made before ovariosteresis (P<0. 05), respectively. However, there was no significant difference, between the group given estrogen/puerarin and the controls, and between the group given estrogen and given puerarin. (P>0. 05). It was suggested that puerarin may have protective effect on the nigral neurons to PD. Moreover, the protective effect might serve as a surrogate of estrogen and be associated with the apoptosis. 展开更多
关键词 Parkinson disease puerarin protection i apoptosis
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Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii 被引量:8
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作者 Ying-Kun Liu E Yan +1 位作者 Han-Ying Zhan Zhi-Qi Zhang 《Journal of Pharmaceutical Analysis》 SCIE CAS 2011年第1期13-19,共7页
Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved b... Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol(1∶15,v/v)for 30 min,and then microwave irradiation for 11 min at a power of 600 W.Coupling the extraction process with HPLC-fluorescence presented good recovery,satisfactory precision,and good linear relation.Compared with a method from the Chinese Pharmacopoeia,the proposed method enables higher extraction efficiency and more accurate analytical results.It can be of potential value in quality assessment of Radix Puerariae thomsonii medicinal materials. 展开更多
关键词 microwave-assisted extraction(MAE) response surface methodology(RSM) puerarin DAIDZEIN HPLC-fluorescence detection Radix Puerariae thomsonii
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Toxicity of endogenous peroxynitrite and effects of puerarin on transplanted retinal pigment epithelial sheets in the subretinal space in mice 被引量:3
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作者 Li-Na Hao, Shou-Zhi He 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2011年第3期250-254,共5页
AIM: To evaluate the toxicity of endogeneous peroxynitrite on transplanted retinal pigment epithelial (RPE) sheets and the effect of puerarin on their survival in the C57BL/6 mice after RPE sheets have been transplant... AIM: To evaluate the toxicity of endogeneous peroxynitrite on transplanted retinal pigment epithelial (RPE) sheets and the effect of puerarin on their survival in the C57BL/6 mice after RPE sheets have been transplanted into SD rats' subretinal space. METHODS: C57BL/6 mice eyes were used to culture RPE cells. Ninety-six SD rats were involved in the experiment. They were divided into control( block control), streptozotocin (STZ, negative control), untransplanted RPE (positive control) and transplanted RPE groups respectively. Diabetes was induced in SD rats by intra-peritoneal STZ injection in the latter three groups. Saline was injected into the subretinal space of 24 SD rats in the untransplanted RPE group and primary RPE sheets were injected into the subretinal space of 24 SD rats in the transplanted RPE group. Puerarin (45 mg/kg) was administrated into both untransplanted RPE and transplanted RPE groups of diabetic rats through intra-peritoneal injection route after RPE sheets transplantation. At 20,40,60 days after surgery, Western blotting analysis, DNA ladder and RT-PCR were used for determining the differences in expression of nitrotyrosine (NT, the foot print of peroxynitrite), apoptosis and iNOS mRNA in the control, STZ, untransplanted RPE and transplanted RPE groups respectively. HE staining was used for determining the RPE survival in the subretinal space of the transplanted RPE group. RESULTS: Apoptosis and expression of NT and iNOS mRNA were observed in STZ, untransplanted RPE and transplanted RPE groups, but were delayed in untransplanted RPE and transplanted RPE groups in a time-dependent manner compared with control and STZ groups (P < 0.01). There were no differences between the two groups (P > 0.01). NT, DNA ladder, iNOS mRNA were down-regulated, which were associated with the decrease of expression of peroxynitrite. Numerous pigmented cells emerged and increased in number in the subretinal space during the 60-day observation period after transplantation. On day 20, heavily pigmented cells were visible at the transplant site; On day 40, monolayer and multilayered transplant was visible in the subretinal space; On day 60, heavily pigmented monolayer and multilayered transplants with round apical profile were present along Bruch's membrane. CONCLUSION: Puerarin increased the 60-day survival of C57BL/6 mice RPE xenografts in the SD rats' subretinal space, which may be related to its direct inhibition of apoptosis of RPE cells and antagnism of damage of peroxynitrite to RPE cells. 展开更多
关键词 retinal pigment epithelial sheets transpantation oxidative puerarin
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Effect of puerarin on myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome 被引量:3
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作者 Ling Niu Dongye Li Yong Xia Defeng Pan Xiaoping Wang Yan Yan Li Liang Tongda Xu Cardiovascular Institute of Xuzhou Medical College, Department of Cardiology, Affiliated Hospital, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2008年第3期155-158,共4页
Objective To investigate the effects of puerarin (Pur) on myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome (ACS).Methods Thirty-seven patients with ACS were randomly divided in... Objective To investigate the effects of puerarin (Pur) on myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome (ACS).Methods Thirty-seven patients with ACS were randomly divided into two groups:conventional treatment group (n= 17,11 males,range of age:32-80 years,average age:60.9±4.9 years) and Pur treatment group (n=20,12 males,range of age:40-76 years,average age:62.7±3.5 years).Patients in the conventional treatment group received standard treatment according to the current guidelines,while patients in the Pur treatment group received intravenous administration of Pur (500 mg/day) for 10 days plus conventional treatment.Real-time myocardial contrast echocardiography (RT-MCE) was performed to evaluate the change in myocardial perfusion index (MPI) and ventricular wall motion index (VWMI) at admission and 10 days after treatment.Results At 10 days after treatment,MPI was significantly higher (P【0.01) and VWMI significantly lower (P【0.01) in the Pur group comparing with those in the conventional group.Conclusions Puerarin might improve myocardial microcirculation perfusion and ventricular wall motion in patients with ACS. 展开更多
关键词 acute CORONARY syndrome MYOCARDIAL PERFUSION INDEX VENTRICULAR wall motion INDEX puerarin
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Synthesis and morphology of triangular pyramid-shaped puerarin microparticle with nanostructure 被引量:3
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作者 Lu, Chuan Hua Zhang, Qun Wei 《Chinese Chemical Letters》 SCIE CAS CSCD 2010年第4期484-487,共4页
A type of triangular pyramid-shaped microparticles of puerarin was synthesized by using oil-in-oil microemulsion approach which is simple and economical under the action of copper substrate.The pyramid-shaped micropar... A type of triangular pyramid-shaped microparticles of puerarin was synthesized by using oil-in-oil microemulsion approach which is simple and economical under the action of copper substrate.The pyramid-shaped microparticles would be made up of deposit of nanospheres or nanorods and have two significant characters.One is its complex surface morphology like coral reef.The other is a lot of nanopores in existence in the microparticle body.Two possible formation routes were speculated. 展开更多
关键词 Triangular pyramid-shaped puerarin microparticles SYNTHESIS NANOSPHERE Nanorod
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