Pharyngitis (an inflammation in the pharynx) is a commonly occurring symptom of upper respiratory tract infection in patients suffering from common cold. The high prevalence of upper respiratory tract infection necess...Pharyngitis (an inflammation in the pharynx) is a commonly occurring symptom of upper respiratory tract infection in patients suffering from common cold. The high prevalence of upper respiratory tract infection necessitates a safe and effective antiinflammatory agent for pharyngitis. Chinese herbal medicine, which has been clinically prescribed for thousands of years, may offer a basis for the treatment of common cold as well as the amelioration of pharyngitis. In the present study, we investigated the in vitro and in vivo anti-inflammatory activities of a Chinese herbal formula, namely GPC, which is comprised of Glycyrrhiza Radix, Platycodonis Radix, Citri Reticulatae Pericarpium, Phyllanthi Fructus and Taraxaci Herba. Incubation with GPC (30, 100 and 300 μg/mL) suppressed the releases of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and nitric oxide in lipopolysaccharide/peptidoglycanactivated RAW264.7 macrophages. In addition to the cell-based study, long-term treatment with GPC (0.35, 1.05 and 2.10 mL/kg/day × 30 doses) was found to reduce the extent of inflammation in animal models of carrageenan-induced paw edema (acute inflammation) as well as cotton pellet-induced granuloma formation (chronic inflammation) in mice. The ability of GPC to enhance the tracheobronchial expectorant action suggested its immunomodulatory activity in the respiratory tract. This postulation was supported by the observation that GPC reduced the degree of pharyngitis and reversed the changes in plasma TNF-α and IL-6 levels in capsaicininduced pharyngitis in rats. The ensemble of results suggests that GPC may offer a promising prospect for alleviating the extent of pharyngitis by virtue of antiinflammatory activities.展开更多
采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-Orbitrap MS)对蒲公英根70%乙醇提取物中的化学成分进行快速鉴定.色谱柱采用Hypersil GOLD aQ色谱柱(100 mm×2.1 mm,1.9μm),并以含0.1%甲酸的水溶液(A液)和含0...采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-Orbitrap MS)对蒲公英根70%乙醇提取物中的化学成分进行快速鉴定.色谱柱采用Hypersil GOLD aQ色谱柱(100 mm×2.1 mm,1.9μm),并以含0.1%甲酸的水溶液(A液)和含0.1%甲酸的乙腈(B液)作为流动相.采用以下梯度进行洗脱:0~2 min 5%B液;2~22 min 5%~95%B液;22~27 min 95%B液;27.1~30 min 5%B液.流速为0.3 mL/min,柱温40℃,进样量为5μL.从蒲公英根中鉴定92个化合物,包括6个氨基酸类、17个苯丙素类、3个核苷类、4个黄酮类、4个醛类、2个生物碱类、4个糖类、11个萜类、3个酰胺类、1个香豆素类、23个有机酸类、14个其他类.本研究对蒲公英根的化学成分进行快速鉴定,为进一步研究蒲公英的药效物质基础和资源开发提供依据.展开更多
文摘Pharyngitis (an inflammation in the pharynx) is a commonly occurring symptom of upper respiratory tract infection in patients suffering from common cold. The high prevalence of upper respiratory tract infection necessitates a safe and effective antiinflammatory agent for pharyngitis. Chinese herbal medicine, which has been clinically prescribed for thousands of years, may offer a basis for the treatment of common cold as well as the amelioration of pharyngitis. In the present study, we investigated the in vitro and in vivo anti-inflammatory activities of a Chinese herbal formula, namely GPC, which is comprised of Glycyrrhiza Radix, Platycodonis Radix, Citri Reticulatae Pericarpium, Phyllanthi Fructus and Taraxaci Herba. Incubation with GPC (30, 100 and 300 μg/mL) suppressed the releases of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6) and nitric oxide in lipopolysaccharide/peptidoglycanactivated RAW264.7 macrophages. In addition to the cell-based study, long-term treatment with GPC (0.35, 1.05 and 2.10 mL/kg/day × 30 doses) was found to reduce the extent of inflammation in animal models of carrageenan-induced paw edema (acute inflammation) as well as cotton pellet-induced granuloma formation (chronic inflammation) in mice. The ability of GPC to enhance the tracheobronchial expectorant action suggested its immunomodulatory activity in the respiratory tract. This postulation was supported by the observation that GPC reduced the degree of pharyngitis and reversed the changes in plasma TNF-α and IL-6 levels in capsaicininduced pharyngitis in rats. The ensemble of results suggests that GPC may offer a promising prospect for alleviating the extent of pharyngitis by virtue of antiinflammatory activities.
文摘采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-Orbitrap MS)对蒲公英根70%乙醇提取物中的化学成分进行快速鉴定.色谱柱采用Hypersil GOLD aQ色谱柱(100 mm×2.1 mm,1.9μm),并以含0.1%甲酸的水溶液(A液)和含0.1%甲酸的乙腈(B液)作为流动相.采用以下梯度进行洗脱:0~2 min 5%B液;2~22 min 5%~95%B液;22~27 min 95%B液;27.1~30 min 5%B液.流速为0.3 mL/min,柱温40℃,进样量为5μL.从蒲公英根中鉴定92个化合物,包括6个氨基酸类、17个苯丙素类、3个核苷类、4个黄酮类、4个醛类、2个生物碱类、4个糖类、11个萜类、3个酰胺类、1个香豆素类、23个有机酸类、14个其他类.本研究对蒲公英根的化学成分进行快速鉴定,为进一步研究蒲公英的药效物质基础和资源开发提供依据.
