目的探索Auto Play Menu Builder软件在多媒体课件制作过程中的应用。方法以《法医物证学》某些章节为例制作课件,展现借助Auto Play Menu Builder制作多媒体课件的过程。结果采用Auto Play Menu Builder软件可制作出含图片、音视频、...目的探索Auto Play Menu Builder软件在多媒体课件制作过程中的应用。方法以《法医物证学》某些章节为例制作课件,展现借助Auto Play Menu Builder制作多媒体课件的过程。结果采用Auto Play Menu Builder软件可制作出含图片、音视频、网页浏览和交互功能等多媒体元素的课件。结论 Auto Play Menu Builder软件可以作为一款制作多媒体课件的新方法之一。展开更多
The Chinese food is well-known in the world as one important part of traditional Chinese culture, which the foreign tourists would like very much to sample and enjoy whenever they visit China. Generally speaking, the ...The Chinese food is well-known in the world as one important part of traditional Chinese culture, which the foreign tourists would like very much to sample and enjoy whenever they visit China. Generally speaking, the foreign tourists also want to make sure, how and with what raw material the Chinese food is cooked while they enjoy it. So the English translation of Chinese menus plays a key role in spreading the tranditional Chinese culture. This paper attempts to explain to readers that: 1)the demands on the English translation of Chinese menus; 2)how to set about translating the Chinese menus; 3)the methods for the English translation of Chinese menus.展开更多
Reception theory stresses reader's activeness.Translation of Chinese Menu in English Version reveals the universal principles of transnational strategies and methods of menu translation.Through analysis of Chinese...Reception theory stresses reader's activeness.Translation of Chinese Menu in English Version reveals the universal principles of transnational strategies and methods of menu translation.Through analysis of Chinese Menu in English Version,the article studies its text and examines the linking mechanism between reception theory and menu translation.A comparative conclusion shows that in menu translation attention is paid to the accuracy of the target text by translators,who are simultaneously aware of the paradox between expectation and acceptance of individual readers.展开更多
文摘目的探索Auto Play Menu Builder软件在多媒体课件制作过程中的应用。方法以《法医物证学》某些章节为例制作课件,展现借助Auto Play Menu Builder制作多媒体课件的过程。结果采用Auto Play Menu Builder软件可制作出含图片、音视频、网页浏览和交互功能等多媒体元素的课件。结论 Auto Play Menu Builder软件可以作为一款制作多媒体课件的新方法之一。
文摘The Chinese food is well-known in the world as one important part of traditional Chinese culture, which the foreign tourists would like very much to sample and enjoy whenever they visit China. Generally speaking, the foreign tourists also want to make sure, how and with what raw material the Chinese food is cooked while they enjoy it. So the English translation of Chinese menus plays a key role in spreading the tranditional Chinese culture. This paper attempts to explain to readers that: 1)the demands on the English translation of Chinese menus; 2)how to set about translating the Chinese menus; 3)the methods for the English translation of Chinese menus.
文摘Reception theory stresses reader's activeness.Translation of Chinese Menu in English Version reveals the universal principles of transnational strategies and methods of menu translation.Through analysis of Chinese Menu in English Version,the article studies its text and examines the linking mechanism between reception theory and menu translation.A comparative conclusion shows that in menu translation attention is paid to the accuracy of the target text by translators,who are simultaneously aware of the paradox between expectation and acceptance of individual readers.
文摘【目的】含有VQ基序(VQ)的蛋白质是植物特异性蛋白质,具有保守的“FxxhVQxhTG”氨基酸序列,调节植物生长和发育。番茄SlMPK1在高温胁迫过程中发挥重要作用,酵母双杂交(Y2H)显示SlVQ6蛋白能够与SlMPK1相互作用,通过GST pull-down技术进一步验证SlMPK1与SlVQ6的互作以及SlVQ6的互作网络,为研究SlVQ6介导的高温应答信号通路奠定基础。【方法】通过pGEX-4T-1-PC-VQ6质粒构建,以含有目的基因SIVQ6的菌液为模板,设计特异性引物,扩增目的基因序列,将获得的目的基因VQ6克隆到载体pGEX-4T-1的BamHⅠ和NotⅠ的酶切位点之间,将获得的重组质粒pGEX-4T-1-PC-VQ6转入TOP10克隆菌株经IPTG(异丙基硫代半乳糖苷)诱导表达,通过GST柱亲和纯化获得目标蛋白PC-VQ6,进而获得预期GST-SlVQ6融合蛋白,通过体外磷酸化进一步确定SlVQ6是否是SlMPK1的下游底物;以GST-SlVQ6为诱饵蛋白固定于GST Sepharose Beads上,与番茄叶片总蛋白孵育后洗脱,收集洗脱液进行SDS-PAGE凝胶电泳验证,通过LC-MS/MS检测SlVQ6可能互作的候选蛋白,并对筛选的SIVQ6互作蛋白通过GO、KEGG和蛋白互作网络进行生物信息学分析。【结果】成功构建pGEX-4T-1-PC-SlVQ6基因重组表达质粒,并获得带有GST标签的GST-SlVQ6融合蛋白,其分子量大小约为54 kD;将GST-SlVQ6和His-SlMPK1进行体外磷酸化试验,SlMPK1能够磷酸化SlVQ6,而作为阴性对照的GST与SlMPK1无相互作用,且SlVQ6不存在自磷酸化的现象,表明GST-SlVQ6和His-SlMPK1具有相互作用,且SlVQ6是SlMPK1的下游底物;以GST-SlVQ6融合蛋白为诱饵蛋白(空GST为阴性对照),利用pull-down试验筛选番茄叶片组织蛋白中与SlVQ6蛋白结合的蛋白,经SDS-PAGE分离、液相色谱-串联质谱(LC-MS/MS)鉴定以及Mascot与蛋白数据库检索,共鉴定出37个与SlVQ6结合的蛋白,包括蛋白激酶Receptor for Activated C Kinase 1B(RACK1B),通过GO、KEGG和蛋白互作网络的生物信息学分析,表明这些蛋白参与多种信号通路,其中8个核糖体蛋白可能与高温胁迫密切相关。【结论】SlVQ6是SlMPK1的底物蛋白,且37个蛋白可能与SlVQ6互作,这些蛋白与胁迫反应有着密切的联系,可能会在提高番茄植株高温耐受性等方面发挥重要作用。
