Increased levels of surfactant protein D (SP-D) and lipid-laden foamy macrophages (FMs) are frequently found under oxidative stress conditions and/or in patients with chronic obstructive pulmonary disease (COPD) who a...Increased levels of surfactant protein D (SP-D) and lipid-laden foamy macrophages (FMs) are frequently found under oxidative stress conditions and/or in patients with chronic obstructive pulmonary disease (COPD) who are also chronically exposed to cigarette smoke (CS). However, the roles and molecular mechanisms of SP-D and FMs in COPD have not yet been determined. In this study, increased levels of SP-D were found in the bronchoalveolar lavage fluid (BALF) and sera of ozone- and CS-exposed mice. Furthermore, SP-D-knockout mice showed increased lipid-laden FMs and airway inflammation caused by ozone and CS exposure, similar to that exhibited by our study cohort of chronic smokers and COPD patients. We also showed that an exogenous recombinant fragment of human SP-D (rfhSP-D) prevented the formation of oxidized low-density lipoprotein (oxLDL)-induced FMs in vitro and reversed the airway inflammation and emphysematous changes caused by oxidative stress and CS exposure in vivo. SP-D upregulated bone marrow-derived macrophage (BMDM) expression of genes involved in countering the oxidative stress and lipid metabolism perturbations induced by CS and oxLDL. Our study demonstrates the crucial roles of SP-D in the lipid homeostasis of dysfunctional alveolar macrophages caused by ozone and CS exposure in experimental mouse emphysema, which may provide a novel opportunity for the clinical application of SP-D in patients with COPD.展开更多
目的观察血清表面活性蛋白D(surfactant protein D,SP-D)在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)急性加重期及缓解期的水平变化及其与ADO指数的相关性,研究血清SP-D水平对于COPD患者预后的指导意义。方法收集3...目的观察血清表面活性蛋白D(surfactant protein D,SP-D)在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)急性加重期及缓解期的水平变化及其与ADO指数的相关性,研究血清SP-D水平对于COPD患者预后的指导意义。方法收集33名COPD急性加重期患者年龄、性别、身高、体质量等基本资料,进行肺功能检查及改良版英国医学研究会呼吸问卷(modified version of the British medical research council respiratory questionnaire,mMRC),分别于入院第2天及经治疗急性加重症状缓解后取血用酶联免疫吸附法(ELISA)测定SP-D水平,并将血清SP-D水平与年龄等进行相关性分析。结果经过治疗后,缓解期COPD患者血清SP-D水平较急性加重期有显著下降(P<0.05);急性加重期血清SP-D水平与ADO指数无相关性,缓解期血清SP-D水平与ADO指数具有负相关性。结论血清SP-D水平可以反映COPD疾病的活动性,可以作为检测COPD是否处于急性加重期的标记物;COPD稳定期患者的血清SP-D水平可以作为评估预后的指标。展开更多
【目的】探讨血清涎液化糖链抗原(Krebs von den Lungen-6,KL-6)、肺表面活性物质-A(Surfactant protein A,SP-A)及肺表面活性物质-D(Surfactant protein D,SP-D)对儿童间质性肺疾病(Inter_(s)titial lung disease,ILD)早期诊断及预后...【目的】探讨血清涎液化糖链抗原(Krebs von den Lungen-6,KL-6)、肺表面活性物质-A(Surfactant protein A,SP-A)及肺表面活性物质-D(Surfactant protein D,SP-D)对儿童间质性肺疾病(Inter_(s)titial lung disease,ILD)早期诊断及预后的评估价值。【方法】选取2016年3月至2020年7月本院呼吸内科诊治的ILD患儿35例(ILD组),另外选取同期在本院诊治感染性肺炎患儿40例(肺炎组)以及40名健康体检儿童作为对照组,检测三组血清KL-6、SP-A、SP-D水平,并分析其对儿童ILD早期诊断及预后评估的价值。【结果】ILD组血清KL-6、SP-A、SP-D水平高于肺炎组和对照组(P<0.01)。ILD患儿血清KL-6水平与最大肺活量(VC_(max))、与一秒率(FEV1/FVC)呈正相关(r_(s)=0.339,P<0.05),SP-A水平与25%最大呼气流量(MEF25)呈负相关(r_(s)=-0.252,P<0.05);SP-D水平与VC_(max)呈负相关(r_(s)=-0.214,P<0.05);肺间质病变评分与KL-6、SP-A、SP-D呈正相关(r_(s)=0.542、0.618、0.515,P<0.01)。KL-6、SP-A、SP-D三者联合诊断ILD的AUC为0.89、敏感性为83.6%、特异性为97.2%、约登指数为0.81,联合检测对ILD的诊断价值显著高于单独检测(P<0.05)。KL-6、SP-A、SP-D为ILD患儿预后的影响因素(P<0.05)。【结论】KL-6、SP-A、SP-D可作为儿童ILD早期诊断、疾病严重程度及预后的评估指标,三者联合应用可提高诊断ILD的准确性。展开更多
基金supported by the Ministry of Science and Technology(MOST)of Taiwan(grant numbers 103-2321-B-006-030 and 104-2321-B-006-008),funding received in part from the Headquarters of University Advancement at the National Cheng Kung University,which is sponsored by the Ministry of Education in Taiwan,and a research grant(1JA8)from the Center for Allergy,Immunology,and Microbiome(A.I.M.),China Medical University Hospital,Taichung,Taiwan.
文摘Increased levels of surfactant protein D (SP-D) and lipid-laden foamy macrophages (FMs) are frequently found under oxidative stress conditions and/or in patients with chronic obstructive pulmonary disease (COPD) who are also chronically exposed to cigarette smoke (CS). However, the roles and molecular mechanisms of SP-D and FMs in COPD have not yet been determined. In this study, increased levels of SP-D were found in the bronchoalveolar lavage fluid (BALF) and sera of ozone- and CS-exposed mice. Furthermore, SP-D-knockout mice showed increased lipid-laden FMs and airway inflammation caused by ozone and CS exposure, similar to that exhibited by our study cohort of chronic smokers and COPD patients. We also showed that an exogenous recombinant fragment of human SP-D (rfhSP-D) prevented the formation of oxidized low-density lipoprotein (oxLDL)-induced FMs in vitro and reversed the airway inflammation and emphysematous changes caused by oxidative stress and CS exposure in vivo. SP-D upregulated bone marrow-derived macrophage (BMDM) expression of genes involved in countering the oxidative stress and lipid metabolism perturbations induced by CS and oxLDL. Our study demonstrates the crucial roles of SP-D in the lipid homeostasis of dysfunctional alveolar macrophages caused by ozone and CS exposure in experimental mouse emphysema, which may provide a novel opportunity for the clinical application of SP-D in patients with COPD.
文摘目的观察血清表面活性蛋白D(surfactant protein D,SP-D)在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)急性加重期及缓解期的水平变化及其与ADO指数的相关性,研究血清SP-D水平对于COPD患者预后的指导意义。方法收集33名COPD急性加重期患者年龄、性别、身高、体质量等基本资料,进行肺功能检查及改良版英国医学研究会呼吸问卷(modified version of the British medical research council respiratory questionnaire,mMRC),分别于入院第2天及经治疗急性加重症状缓解后取血用酶联免疫吸附法(ELISA)测定SP-D水平,并将血清SP-D水平与年龄等进行相关性分析。结果经过治疗后,缓解期COPD患者血清SP-D水平较急性加重期有显著下降(P<0.05);急性加重期血清SP-D水平与ADO指数无相关性,缓解期血清SP-D水平与ADO指数具有负相关性。结论血清SP-D水平可以反映COPD疾病的活动性,可以作为检测COPD是否处于急性加重期的标记物;COPD稳定期患者的血清SP-D水平可以作为评估预后的指标。
文摘【目的】探讨血清涎液化糖链抗原(Krebs von den Lungen-6,KL-6)、肺表面活性物质-A(Surfactant protein A,SP-A)及肺表面活性物质-D(Surfactant protein D,SP-D)对儿童间质性肺疾病(Inter_(s)titial lung disease,ILD)早期诊断及预后的评估价值。【方法】选取2016年3月至2020年7月本院呼吸内科诊治的ILD患儿35例(ILD组),另外选取同期在本院诊治感染性肺炎患儿40例(肺炎组)以及40名健康体检儿童作为对照组,检测三组血清KL-6、SP-A、SP-D水平,并分析其对儿童ILD早期诊断及预后评估的价值。【结果】ILD组血清KL-6、SP-A、SP-D水平高于肺炎组和对照组(P<0.01)。ILD患儿血清KL-6水平与最大肺活量(VC_(max))、与一秒率(FEV1/FVC)呈正相关(r_(s)=0.339,P<0.05),SP-A水平与25%最大呼气流量(MEF25)呈负相关(r_(s)=-0.252,P<0.05);SP-D水平与VC_(max)呈负相关(r_(s)=-0.214,P<0.05);肺间质病变评分与KL-6、SP-A、SP-D呈正相关(r_(s)=0.542、0.618、0.515,P<0.01)。KL-6、SP-A、SP-D三者联合诊断ILD的AUC为0.89、敏感性为83.6%、特异性为97.2%、约登指数为0.81,联合检测对ILD的诊断价值显著高于单独检测(P<0.05)。KL-6、SP-A、SP-D为ILD患儿预后的影响因素(P<0.05)。【结论】KL-6、SP-A、SP-D可作为儿童ILD早期诊断、疾病严重程度及预后的评估指标,三者联合应用可提高诊断ILD的准确性。
